• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Diversity of coenzyme Q biosynthetic pathways

Research Project

Project/Area Number 21H02117
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 38030:Applied biochemistry-related
Research InstitutionShimane University

Principal Investigator

KAWAMUKAI MAKOTO  島根大学, 学術研究院農生命科学系, 教授 (70186138)

Co-Investigator(Kenkyū-buntansha) 西田 郁久  新潟大学, 日本酒学センター, 特任助教 (20784531)
中澤 昌美  大阪公立大学, 大学院農学研究科, 講師 (90343417)
戒能 智宏  島根大学, 学術研究院農生命科学系, 准教授 (90541706)
Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2023: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2022: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2021: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
KeywordsコエンザイムQ / ユビキノン / 分裂酵母 / ミトコンドリア / 電子伝達系 / 抗酸化物質 / 補酵素 / 生合成 / coenzyme Q / ubiquinone / fission yeast / S. pombe / 酵母 / 硫化水素 / ロドキノン / PHB
Outline of Research at the Start

コエンザイムQは生体内において電子伝達系の必須成分として重要な機能を果たしていると同時に抗酸化物質としての機能が注目されている。CoQが生体で重要な物質であることは、ヒトの遺伝病とCoQの関連性が蓄積していることからも明らかである。一方で、実際はCoQ生合成経路が未だ完全解明されていない。むしろ我々のこれまでの解析により、PABAを介した経路など複数の経路が合成系に関わるという多様性を示す知見を得ている。未知の反応の解明、とりわけキノン骨格の上流の反応とキノンの形成反応に焦点をあて、その知見をヒトのCoQ生合成経路への理解へと拡張することを目標としている。

Outline of Final Research Achievements

Coenzyme Q (ubiquinone, CoQ) plays a function as an essential component of the electron transport system in a wide range of organisms, and at the same time, it functions as a cofactor for various enzymes and an antioxidant. It gains an attracted attention as a food supplement. Although CoQ is a substance that plays an essential function in mitochondria for ATP synthesis in the electron transport system, its biosynthetic pathway has not been fully elucidated. Recent studies have gradually revealed that numerous enzymes are involved in the CoQ synthetic pathway, rather than we initially thought. In this study, fission yeast Schizosaccharomyces pombe, which biosynthesizes CoQ10, was used to conduct research to elucidate the full scope of biosynthesis and to link the findings to an understanding of human CoQ biosynthesis.

Academic Significance and Societal Importance of the Research Achievements

分裂酵母は、人と同じCoQ10を合成する酵母として、かつ遺伝学的な解析が容易な微生物として、CoQ10生産を考える上でも、生合成経路を理解する面においても重要な研究対象である。CoQ生合成の上流に位置する共通の初発化合物であるPHBは、Coq2タンパク質によって、イソプレニル鎖と縮合される際の基質となるが、PHBの合成経路に関しては、著しく知見が不足しており、チロシンから何段階を経て合成されるのかも解明されていない。今回の我々の発見において、CoQ生合成の上流合成経路に関わるCoq12を発見することができたことは、この成果が人を含めた他の生物でのCoQ生合成の理解に広がる意義を有する。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • Research Products

    (25 results)

All 2024 2023 2022 2021

All Journal Article (10 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 10 results,  Open Access: 7 results) Presentation (14 results) (of which Int'l Joint Research: 1 results,  Invited: 5 results) Book (1 results)

  • [Journal Article] Identification of novel coenzyme Q10 biosynthetic proteins Coq11 and Coq12 in Schizosaccharomyces pombe2023

    • Author(s)
      Ikuhisa Nishida, Yuki Ohmori , Ryota Yanai, Shogo Nishihara, Yasuhiro Matsuo, Tomohiro Kaino , Dai Hirata , Makoto Kawamukai
    • Journal Title

      J. Biol. Chem

      Volume: - Issue: 6 Pages: 104797-104797

    • DOI

      10.1016/j.jbc.2023.104797

    • Related Report
      2023 Annual Research Report 2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Tfs1, transcription elongation factor TFIIS, has an impact on chromosome segregation affected by pka1 deletion in Schizosaccharomyces pombe2023

    • Author(s)
      Takenaka Kouhei、Nishioka Shiho、Nishida Yuki、Kawamukai Makoto、Matsuo Yasuhiro
    • Journal Title

      Current Genetics

      Volume: 69 Issue: 2-3 Pages: 115-125

    • DOI

      10.1007/s00294-023-01268-0

    • Related Report
      2023 Annual Research Report 2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Direct Visualization of Structurally Similar Polysaccharides in Single Yeast Cells <i>in Vivo</i> by Multivariate Analysis Assisted Raman Microspectroscopy2023

    • Author(s)
      Hossain Imrul M.、Mazumder Nirmal、Kaino Tomohiro、Kawamukai Makoto、Noothalapati Hemanth、Yamamoto Tatsuyuki
    • Journal Title

      The Journal of Physical Chemistry B

      Volume: 127 Issue: 23 Pages: 5249-5256

    • DOI

      10.1021/acs.jpcb.3c00632

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A dominant negative 14-3-3 mutant in Schizosaccharomyces pombe distinguishes the binding proteins involved in sexual differentiation and check point2023

    • Author(s)
      Ohshima Tomohito、Jiajun Zhang、Fukamachi Takuki、Ohno Yuko、Senoo Hiroko、Matsuo Yasuhiro、Kawamukai Makoto
    • Journal Title

      PLOS ONE

      Volume: 18 Issue: 10 Pages: e0291524-e0291524

    • DOI

      10.1371/journal.pone.0291524

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Whole-Genome Sequencing of <i>Pseudoalteromonas</i> sp. Strain KAN5, an Agar-Degrading Bacterium Isolated from the Gut of an Alga-Eating Fish2022

    • Author(s)
      Kawase Yuka、Tanabe Takuma、Kawamukai Makoto、Yoshida Masa-aki
    • Journal Title

      Microbiology Resource Announcements

      Volume: 11 Issue: 8

    • DOI

      10.1128/mra.00398-22

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Heptaprenyl Diphosphate Synthase (Coq1) Is the Target of a Lipophilic Bisphosphonate That Protects Mice against Toxoplasma gondii Infection2022

    • Author(s)
      Sleda Melissa A.、Li Zhu-Hong、Behera Ranjan、Baierna Baihetiya、Li Catherine、Jumpathong Jomkwan、Malwal Satish R.、Kawamukai Makoto、Oldfield Eric、Moreno Silvia N. J.
    • Journal Title

      mBio

      Volume: 13 Issue: 5

    • DOI

      10.1128/mbio.01966-22

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Synergistic roles of the phospholipase B homolog Plb1 and the cAMP-dependent protein kinase Pka1 in the hypertonic stress response of Schizosaccharomyces pombe2022

    • Author(s)
      Matsuo Yasuhiro、Marcus Stevan、Kawamukai Makoto
    • Journal Title

      Current Genetics

      Volume: 68 Issue: 5-6 Pages: 661-674

    • DOI

      10.1007/s00294-022-01253-z

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gut microbiota analysis of Blenniidae fishes including an algae-eating fish and clear boundary formation among isolated Vibrio strains2022

    • Author(s)
      Yoshida Masa-aki、Tanabe Takuma、Akiyoshi Hideo、Kawamukai Makoto
    • Journal Title

      Scientific Reports

      Volume: 12 Issue: 1

    • DOI

      10.1038/s41598-022-08511-7

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Expression of Mug14 is regulated by the transcription factor Rst2 through the cAMP-dependent protein kinase pathway in Schizosaccharomyces pombe2021

    • Author(s)
      Inamura Shin-ich、Tanabe Takuma、Kawamukai Makoto、Matsuo Yasuhiro
    • Journal Title

      Current Genetics

      Volume: 67 Issue: 5 Pages: 807-821

    • DOI

      10.1007/s00294-021-01194-z

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genome-wide Screening of Genes Associated with Momilactone B Sensitivity in the Fission Yeast2021

    • Author(s)
      Keisuke Tomita, Yoko Yashiroda, Yasuhiro Matsuo, Jeff S Piotrowski, Sheena C Li, Reika Okamoto, Mami Yoshimura, Hiromi Kimura, Yumi Kawamura, Makoto Kawamukai, Charles Boone, Minoru Yoshida, Hideaki Nojiri, Kazunori Okada
    • Journal Title

      G3 (Bethesda)

      Volume: 11 Issue: 8

    • DOI

      10.1093/g3journal/jkab156

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] cAMPとCoQ10に惹かれて2024

    • Author(s)
      川向 誠
    • Organizer
      日本農芸化学会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] 分裂酵母のミトコンドリア局在型NAD(H) kinase Pos5はCoQ合成に関与する2024

    • Author(s)
      西原昇瑚 西田郁久 柳井良太 松尾安浩 戒能智宏 川向 誠
    • Organizer
      日本農芸化学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 分裂酵母を基盤とした増殖制御機構とCoQ10生合成の研究2023

    • Author(s)
      川向 誠
    • Organizer
      日本農芸化学会中四国西日本合同大会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] コエンザイムQ10の新機能、生合成と生産2023

    • Author(s)
      川向 誠
    • Organizer
      第24回酵母合同シンポジウム
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] cAMPからCoQ10まで2023

    • Author(s)
      川向 誠
    • Organizer
      第40回イーストワークショップ
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] Biosynthesis and bioproduction of Coenzyme Q in yeasts2023

    • Author(s)
      Makoto Kawamukai
    • Organizer
      Cold Sring Harbor Asia
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] S. pombeのCoQ10生合成に関与するCoq11及びCoq12の解析2023

    • Author(s)
      西田 郁久、大森 夕貴、柳井 良太、西野 耕平、西原 昇瑚、松尾 安浩、戒能 智宏、 平田 大、川向 誠
    • Organizer
      日本農芸化学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 分裂酵母S. japonicusのCoQ生合成酵素遺伝子Sjcoq3~Sjcoq9の機能性の解析2023

    • Author(s)
      石神 夏萌、楢原 拓之、戒能 智宏、川向 誠
    • Organizer
      日本農芸化学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 分裂酵母S.pombeのcoq6およびcoq7破壊株へのキノン骨格類縁体添加によるCoQ合成への影響2022

    • Author(s)
      上西倫太郎、堀知葉、松本早代、戒能智宏、川向 誠
    • Organizer
      日本農芸化学会中四国支部例会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 分裂酵母S. japonicusのCoQ生合成のキノン骨格修飾に関与する遺伝子の相補性の解析2022

    • Author(s)
      石神 夏萌、楢原 拓之、戒能 智宏、川向 誠
    • Organizer
      日本農芸化学会中四国支部例会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 分裂酵母の Php 複合体が,CoQ 合成に関わる 4-ヒドロキシ安息香酸(4-HB)合成経路に及ぼす影響2022

    • Author(s)
      高塚こまち,松浦啓太,柳井良太,西原昇瑚 ,戒能智宏, 川向 誠
    • Organizer
      日本農芸化学会中四国支部例会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 分裂酵母の転写因子の高発現とキノン骨格基質の添加によるCoQ生合成への影響の解析2022

    • Author(s)
      高塚 こまち、角 陽香、松浦 啓太、柳井 良太、西原 昇瑚、戒能 智宏、川向 誠
    • Organizer
      日本農芸化学会2022大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] NADHキナーゼPoS5は分裂酵母と出芽酵母のCoQ生合成に関与する2021

    • Author(s)
      西原 昇瑚、西田郁久、柳井 良太、松尾安浩、戒能 智宏、川向 誠
    • Organizer
      第18回日本コエンザイムQ協会研究会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 分裂酵母S. pombe および出芽酵母S. cerevisiae の NADH キナーゼ Pos5 と CoQ 生合成との関連性の解析2021

    • Author(s)
      西原 昇瑚、西田郁久、柳井 良太、松尾安浩、戒能 智宏、川向 誠
    • Organizer
      第31回イソプレノイド研究会
    • Related Report
      2021 Annual Research Report
  • [Book] ビタミン・バイオファクター総合事典 分担「コエンザイムQの生合成と欠損症」2021

    • Author(s)
      川向誠
    • Total Pages
      672
    • Publisher
      朝倉書店
    • ISBN
      9784254102925
    • Related Report
      2021 Annual Research Report

URL: 

Published: 2021-04-28   Modified: 2025-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi