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Molecular analysis of the extremely rare and high-needs cytoplasmic male sterile rice plant with sporophytically-controlled indehiscent anthers

Research Project

Project/Area Number 21H02161
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 39010:Science in plant genetics and breeding-related
Research InstitutionTohoku University

Principal Investigator

Toriyama Kinya  東北大学, 農学研究科, 教授 (20183882)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2023: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2022: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2021: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Keywords育種学 / 遺伝学 / 遺伝子 / ゲノム / 植物 / イネ
Outline of Research at the Start

ハイブリッドライスの育種に胞子体型の細胞質雄性不稔性/稔性回復システム(CMS/RF)の利用が望まれているが、現在日本型のイネに利用できるシステムがない。本研究では我々がインディカ品種Tadukan から見出した胞子体型のCMS /RF系統について、ミトコンドリアのCMS原因遺伝子の同定とゲノム編集を用いた証明、及び、稔性回復遺伝子のマップベースクローニングを行い、雄性不稔・稔性回復の分子基盤を明らかにする。

Outline of Final Research Achievements

Cytoplasmic male sterility (CMS; the phenomenon of pollen death due to incompatibility between the mitochondrial and nuclear genomes) is used in the breeding of F1 hybrid varieties. In this study, we found that a japonica rice cultivar carrying the mitochondria of an indica rice variety ‘Tadukan’ shows CMS and fertility restoration. We have identified a CMS-causing gene in the mitochondrial genome and proved by employing genome-editing. We have also identified a nuclear gene responsible for fertility restoration.

Academic Significance and Societal Importance of the Research Achievements

一代雑種品種は、F1が両親に比べ旺盛な生育を示す性質を利用したもので、市販されているほとんどの野菜、トウモロコシ、ソルガムなどは一代雑種品種であり、その育種に細胞質雄性不稔性(CMS;ミトコンドリアゲノムと核ゲノムの相性が悪く花粉が死滅する現象)が使われている。我々が発見したインディカ品種Tadukanに由来するCMS系統は胞子体型の稔性回復様式を示す極めて稀なタイプであり、しかも育種の現場ニーズがあるCMS /RFシステムである。花粉の発育不良ではなく、葯の裂開不良が原因のCMSイネであり、ジャポニカイネの一代雑種品種の育種に利用できる。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • Research Products

    (33 results)

All 2024 2023 2022 2021

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 5 results) Presentation (27 results) (of which Int'l Joint Research: 5 results,  Invited: 3 results) Book (1 results)

  • [Journal Article] The mitochondrial and plastid genomes of <i>Oryza sativa</i> L. cv. Taichung 652023

    • Author(s)
      Ichida Hiroyuki、Kazama Tomohiko、Arimura Shin-ichi、Toriyama Kinya
    • Journal Title

      Plant Biotechnology

      Volume: 40 Issue: 1 Pages: 109-112

    • DOI

      10.5511/plantbiotechnology.22.1213a

    • ISSN
      1342-4580, 1347-6114
    • Year and Date
      2023-03-25
    • Related Report
      2023 Annual Research Report 2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] <scp>TALEN</scp> ‐mediated depletion of the mitochondrial gene <i>orf312</i> proves that it is a Tadukan‐type cytoplasmic male sterility‐causative gene in rice2022

    • Author(s)
      Takatsuka Ayumu、Kazama Tomohiko、Arimura Shin‐ichi、Toriyama Kinya
    • Journal Title

      The Plant Journal

      Volume: 未定 Issue: 4 Pages: 1-11

    • DOI

      10.1111/tpj.15715

    • Related Report
      2022 Annual Research Report 2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular basis of cytoplasmic male sterility and fertility restoration in rice2021

    • Author(s)
      Toriyama K
    • Journal Title

      Plant Biotechnology

      Volume: 38 Issue: 3 Pages: 285-295

    • DOI

      10.5511/plantbiotechnology.21.0607a

    • NAID

      130008091610

    • ISSN
      1342-4580, 1347-6114
    • Year and Date
      2021-09-25
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cytoplasmic male sterility-associated mitochondrial gene orf312 derived from rice (Oryza sativa L.) cultivar Tadukan2021

    • Author(s)
      Takatsuka A, Kazama T and Toriyama K
    • Journal Title

      Rice

      Volume: 14 Issue: 1 Pages: 1-11

    • DOI

      10.1186/s12284-021-00488-7

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Disruption of mitochondrial open reading frame 352 partially restores pollen development in cytoplasmic male sterile rice2021

    • Author(s)
      Shiho Omukai, Shin-ich Arimura, Kinya Toriyama, Tomohiko Kazama
    • Journal Title

      Plant Physiology

      Volume: 187 Issue: 1 Pages: 236-246

    • DOI

      10.1093/plphys/kiab236

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Mitochondria-targeted TALEN-mediated gene knockout and reorganization of mitochondrial genome in Tadukan-type cytoplasmic male sterile rice2024

    • Author(s)
      Ayumu Takatsuka, Tomohiko Kazama, Shin-ichi Arimura, Kinya Toriyama
    • Organizer
      第65回日本植物生理学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] ミトコンドリア遺伝子orf312により雄性生殖器官の機能が阻害されるイネの形態観察とトランスクリプトーム解析2023

    • Author(s)
      髙塚歩, 風間智彦, 鳥山欽哉
    • Organizer
      日本生化学会東北支部 第89回例会・シンポジウム
    • Related Report
      2023 Annual Research Report
  • [Presentation] インディカイネTadukanに由来するミトコンドリア遺伝子が葯の裂開を阻害するメカニズムの解析2023

    • Author(s)
      髙塚歩, 風間智彦, 鳥山欽哉
    • Organizer
      イネ遺伝学・分子生物学ワークショップ2023
    • Related Report
      2023 Annual Research Report
  • [Presentation] イネ品種Tadukanに由来する細胞質雄性不稔性イネの形態観察およびトランスクリプトーム解析2023

    • Author(s)
      髙塚歩, 風間智彦, 鳥山欽哉
    • Organizer
      第40回日本植物バイオテクノロジー学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 形態観察とトランスクリプトーム解析によるTadukan型細胞質雄性不稔性の分子機構考察2023

    • Author(s)
      髙塚歩, 風間智彦, 鳥山欽哉
    • Organizer
      第144回日本育種学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] MitoTALEN-mediated mitochondrial gene knockout and mechanistic analysis of Tadukan-type cytoplasmic male sterile rice2023

    • Author(s)
      Takatsuka A, Kazama T, Arimura S, Toriyama K
    • Organizer
      JSPS core-to-core meeting in France
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Gene identification and mechanistic analysis of cytoplasmic male sterility derived from Oryza sativa cv. Taduka2023

    • Author(s)
      Takatsuka A, Kazama T, Arimura S, Toriyama K
    • Organizer
      6th Plants and People Conference Exploring Plants
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification of a mitochondrial gene causing Tadukan-type cytoplasmic male sterility by the mitochondrial genome reorganization mediated by TALEN2023

    • Author(s)
      Takatsuka A, Kazama T, Arimura S, Toriyama K
    • Organizer
      15th International Association for Plant Biotechnology Congress
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Mitochondrial gene knockout and mechanistic analysis of Tadukan-type cytoplasmic male sterile rice2023

    • Author(s)
      Takatsuka A, Kazama T, Arimura S, Toriyama K
    • Organizer
      6th International Rice Congress
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ミトコンドリア遺伝子 orf312 が葯の裂開を阻害する Tadukan 型細胞質雄性不稔性イネの組織学的解析2023

    • Author(s)
      高塚歩,風間智彦,鳥山欽哉
    • Organizer
      第64回日本植物生理学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] イネの雄性生殖器官の発育不全を引き起こすミトコンドリア遺伝子のゲノム編集2022

    • Author(s)
      髙塚歩, 風間智彦, 有村慎一, 鳥山欽哉
    • Organizer
      日本生化学会東北支部第88回例会・シンポジウム
    • Related Report
      2022 Annual Research Report
  • [Presentation] Tadukanに由来する細胞質雄性不稔性イネの稔性回復候補遺伝子2022

    • Author(s)
      髙塚歩, 風間智彦, 市田裕之, 阿部知子, 鳥山欽哉
    • Organizer
      第39回日本植物バイオテクノロジー学会(堺)大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Tadukan型細胞質雄性不稔性イネを稔性回復させる遺伝子の探索2022

    • Author(s)
      髙塚歩, 風間智彦, 市田裕之, 阿部知子, 鳥山欽哉
    • Organizer
      日本育種学会第142回講演会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Exploration for a restorer of fertility gene for cytoplasmic male sterility derived from Oryza sativa cv. Tadukan2022

    • Author(s)
      Ayumu Takatsuka, Tomohiko Kazama, Hiroyuki Ichida,Tomoko Abe, Kinya Toriyama
    • Organizer
      第17回東北育種研究集会
    • Related Report
      2022 Annual Research Report
  • [Presentation] イネのミトコンドリア遺伝性の雄性生殖器官発育不全と核のレスキュー遺伝子2022

    • Author(s)
      髙塚歩, 風間智彦, 市田裕之, 阿部知子, 鳥山欽哉
    • Organizer
      第45回日本分子生物学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Tadukan型細胞質雄性不稔性イネのミトコンドリアRNAプロセッシングに関わる稔性回復遺伝子の推定2022

    • Author(s)
      髙塚歩, 風間智彦, 市田裕之, 阿部知子, 鳥山欽哉
    • Organizer
      第10回植物RNA研究ネットワークシンポジウム
    • Related Report
      2022 Annual Research Report
  • [Presentation] Tadukan型細胞質雄性不稔性イネの花粉発達は正常だが葯の裂開が阻害され、稔性回復は胞子体型に行なわれる2022

    • Author(s)
      髙塚歩, 風間智彦, 鳥山欽哉
    • Organizer
      日本育種学会第141回講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ミトコンドリア移行TALENを用いたイネ品種Tadukanにおける雄性不稔遺伝子の機能証明2021

    • Author(s)
      高塚歩, 風間智彦, 有村慎一, 鳥山欽哉
    • Organizer
      日本ゲノム編集学会第6回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 私もできる植物ゲノム編集―CRISPR-Cas9 と TALEN を用いた遺伝子破壊の研究例―2021

    • Author(s)
      鳥山欽哉
    • Organizer
      園芸学会東北支部令和3年度大会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] イネの細胞質雄性不稔性と稔性回復の分子基盤2021

    • Author(s)
      鳥山欽哉
    • Organizer
      日本植物バイオテクノロジー学会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 植物ミトコンドリアゲノム編集技術の開発と細胞質雄性不稔原因遺伝子の同定2021

    • Author(s)
      風間智彦・肥塚信也・鳥山欽哉・堤伸浩・有村慎一
    • Organizer
      日本植物バイオテクノロジー学会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] mitoTALEN導入によるTadukan型細胞質雄性不稔性イネにおけるゲノム再構成の誘発2021

    • Author(s)
      高塚歩, 風間智彦, 有村慎一, 鳥山欽哉
    • Organizer
      日本植物バイオテクノロジー学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ミトコンドリア移行TALENによるCW型細胞質雄性不稔イネ原因遺伝子の同定2021

    • Author(s)
      木下諄美, 有村慎一, 鳥山 欽哉, 風間智彦
    • Organizer
      日本植物バイオテクノロジー学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] イネTA-CMSにおいてミトコンドリア移行TALENが引き起こす相同組換え修復およびゲノム構成の変化の解析2021

    • Author(s)
      高塚歩, 風間智彦, 有村慎一, 鳥山欽哉
    • Organizer
      日本育種学会第140回講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ミトコンドリア移行TALEN導入で再構成されたイネミトコンドリアゲノムの次世代シーケンス解析2021

    • Author(s)
      髙塚歩, 風間智彦, 有村慎一, 鳥山欽哉
    • Organizer
      第16回東北育種研究集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] イネ品種Tadukanに由来するミトコンドリア雄性不稔遺伝子のmitoTALENによるノックアウトと後代の安定性2021

    • Author(s)
      髙塚歩, 風間智彦, 有村慎一, 鳥山 欽哉
    • Organizer
      第44回日本分子生物学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Identification of a cytoplasmic male sterility causative gene in Oryza sativa cv. Tadukan with mitoTALEN-mediated mitochondrial gene-knockout2021

    • Author(s)
      Takatsuka A, Kazama T, Arimura S, Toriyama K
    • Organizer
      Plant Biology 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Book] 植物バイオテクノロジーの基礎知識2021

    • Author(s)
      伊藤幸博、鳥山欽哉
    • Total Pages
      142
    • Publisher
      東北大学出版会
    • ISBN
      9784861633607
    • Related Report
      2021 Annual Research Report

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Published: 2021-04-28   Modified: 2025-01-30  

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