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Using a symbiotic system between endophytes and native microorganisms can stably suppress soil diseases.

Research Project

Project/Area Number 21H02191
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 39040:Plant protection science-related
Research InstitutionIbaraki University

Principal Investigator

Narisawa Kazuhiko  茨城大学, 農学部, 教授 (90431650)

Co-Investigator(Kenkyū-buntansha) 坂上 伸生  茨城大学, 農学部, 准教授 (00564709)
晝間 敬  東京大学, 大学院総合文化研究科, 准教授 (20714504)
清水 将文  岐阜大学, 応用生物科学部, 准教授 (60378320)
Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2023: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2022: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2021: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Keywordsエンドファイト / 土着微生物 / 共生 / 土壌病害防除
Outline of Research at the Start

植物に内生する根部エンドファイト(DSE) と土着微生物との共生系により圃場でも安定して土壌病害を抑制出来る新技術を提案する。まず環境中などからDSEと相互作用を示す有用なバクテリアを釣餌し、土壌病害に対して安定して抑制効果を示すDSE-バクテリア共生系を選抜する。次に共生や病害抑制など植物-DSE-バクテリア間の相互作用メカニズムを解明する。さらに、野外で植物の病害防除に最適な同共生系をコアとした土着微生物叢を把握することで実用化の基盤技術を構築する。

Outline of Final Research Achievements

A DSE-bacteria symbiosis that enhances the effectiveness of DSE was selected. Furthermore, it was found that the inoculation of DSE and its compatible bacteria during seedling cultivation is effective. It was estimated that the inoculation of DSE and its compatible bacteria significantly promoted plant growth and increased the production of metabolic substances such as flavonoids in the plant roots.
In the seedling roots, the fungal flora in the rhizosphere was changed by treatment with C. chaetospira. Furthermore, during seedling cultivation, Cc showed a higher colonization rate than V. simplex, but in the field, Vs maintained a higher colonization rate. Fungi that grew with both DSEs, and fungi and bacteria that grew only with Vs or only with Cc were identified and listed.

Academic Significance and Societal Importance of the Research Achievements

単独生物に注目するのではなく、植物-DSE-バクテリアを1つの系としてとらえ、その相互作用を明らかにし、利用することで、これまで圃場での効果が不安定であった有用微生物の利用技術にブレークスルーをもたらすことが出来る。さらに、我々が菌類として認識していた生物が、生態系では、いわば菌類とバクテリアの共生体として振る舞うという、これまでの菌類学の概念を覆し、新たな生物共生系の存在を示すことになる。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • Research Products

    (14 results)

All 2024 2023 2022 2021

All Journal Article (6 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results) Presentation (7 results) Book (1 results)

  • [Journal Article] Isolation, structural elucidation, and biological activity of a novel isocoumarin from the dark septate endophytic fungus <i>Phialocephala fortinii</i>2024

    • Author(s)
      Bando Kei、Kushibe Ryoga、Kitaoka Naoki、Tamai Yutaka、Narisawa Kazuhiko、Matsuura Hideyuki
    • Journal Title

      Zeitschrift f&#252;r Naturforschung C

      Volume: 79 Issue: 3-4 Pages: 89-92

    • DOI

      10.1515/znc-2023-0139

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dark septate endophytic fungi associated with pioneer grass inhabiting volcanic deposits and their functions in promoting plant growth2023

    • Author(s)
      Sun Han、Nishizawa Tomoyasu、Ohta Hiroyuki、Narisawa Kazuhiko
    • Journal Title

      Biogeosciences

      Volume: 20 Issue: 23 Pages: 4737-4749

    • DOI

      10.5194/bg-20-4737-2023

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] First Report of Tripartite Symbiosis Potential among Soybean, Bradyrhizobium japonicum, and Dark Septate Endophytes2023

    • Author(s)
      Innosensia Ni Luh Putu Citra、Suputra I Putu Wirya、Wirya Gusti Ngurah Alit Susanta、Narisawa Kazuhiko
    • Journal Title

      Agronomy

      Volume: 13 Issue: 7 Pages: 1788-1788

    • DOI

      10.3390/agronomy13071788

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Dark Septate Endophyte, Phialocephala fortiniiJ2PC4, Mitigating Southern Rice Black-Streaked Dwarf Disease and Lethal to White Back Planthopper.2022

    • Author(s)
      YanZhang, BixiaQin, LingXie, KazuhikoNarisawa, QianNong, LipingQin
    • Journal Title

      Biological Control

      Volume: 170 Pages: 104911-104911

    • DOI

      10.1016/j.biocontrol.2022.104911

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Biocontrol of Pythium root rot on lisianthus using a new dark septate endophytic fungus Hyaloscypha variabilis J1PC12022

    • Author(s)
      Marian Malek、Takashima Yusuke、Harsonowati Wiwiek、Murota Haruhiko、Narisawa Kazuhiko
    • Journal Title

      European Journal of Plant Pathology

      Volume: 163 Issue: 1 Pages: 97-112

    • DOI

      10.1007/s10658-022-02459-0

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Prospecting the unpredicted potential traits of Cladophialophora chaetospira SK51 to alter photoperiodic flowering in strawberry, a perennial SD plant2022

    • Author(s)
      Harsonowati Wiwiek、Masrukhin、Narisawa Kazuhiko
    • Journal Title

      Scientia Horticulturae

      Volume: 295 Pages: 110835-110835

    • DOI

      10.1016/j.scienta.2021.110835

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] ゴボウの土壌病害には種子由来の菌類が影響する!-エンドファイトでの防除の取り組み-2023

    • Author(s)
      佐藤佑飛、成澤才彦
    • Organizer
      日本有機農業学会 第24回大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 農作物への重金属の蓄積をエンドファイトは制御できるのか?2023

    • Author(s)
      穂積由樹、成澤才彦
    • Organizer
      日本有機農業学会 第24回大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 茨城県におけるテンサイの生育と共生菌類の接種による耐暑性付与.2022

    • Author(s)
      沼倉千紘, 野口愛, 浅木直美, 坂上伸生, 成澤才彦
    • Organizer
      日本土壌微生物学会 2022年度大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Cladophialophora sp.13nは乾燥ストレス条件下でトウモロコシの生育を促進する2022

    • Author(s)
      森田翔央、成澤才彦
    • Organizer
      日本微生物生態学会 第35回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] トマト苗への根部エンドファイトVeronaeopsis simplex接種が土壌化学性および植物体元素組成に及ぼす影響2021

    • Author(s)
      大好優華・助川歩乃佳・野口 愛・Wibawa IGKS・松浦江里・高田圭太・成澤才彦・坂上伸生
    • Organizer
      日本土壌微生物学会2021年度大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ロボット芝刈機と根部エンドファイトを利用したミニトマトの新しい不耕起草生・有機栽培技術の検討2021

    • Author(s)
      福田 真丈, 高嶋 尚哉, 野口 愛, 成澤 才彦, 坂上 伸生, 小松﨑 将一
    • Organizer
      第79回農業食料工学会(令和3年度)年次大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Dark Septate Endophytesのイネ生育への影響について2021

    • Author(s)
      関根直人,成澤才彦
    • Organizer
      日本有機農業学会 2021年度大会
    • Related Report
      2021 Annual Research Report
  • [Book] 「菌根菌ではないけれど植物と切っても切れない関係のDSE」もっと菌根の世界 : 知られざる根圏のパートナーシップ2023

    • Author(s)
      成澤才彦(齋藤雅典編)
    • Total Pages
      352
    • Publisher
      築地書館
    • ISBN
      9784806716556
    • Related Report
      2023 Annual Research Report

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Published: 2021-04-28   Modified: 2025-01-30  

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