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Characterization of the biotrophic infection process of Rhizoctonia solani in plants

Research Project

Project/Area Number 21H02197
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 39040:Plant protection science-related
Research InstitutionOkayama University

Principal Investigator

Noutoshi Yoshiteru  岡山大学, 環境生命自然科学学域, 教授 (70332278)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2023: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2022: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2021: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Keywords紋枯病 / リゾクトニア病 / 感染生理 / ミナトカモジグサ / 殺生寄生 / 活物寄生 / エフェクター / 殺生菌 / 活物寄生菌 / 紋枯病菌 / イネ / シロイヌナズナ
Outline of Research at the Start

紋枯病は糸状菌Rhizoctonia solaniが引き起こす植物病害であり、イネやトウモロコシなどに重大な被害を及ぼす。病害防除には原因菌の感染生理の理解が不可欠だが、本菌の研究は進んでいない。従来、紋枯病菌は宿主細胞を殺して栄養を摂取すると考えられてきたが、我々はこれまでの研究により、紋枯病菌がその感染初期に生きた宿主から栄養を摂取する活物寄生というステージを経るという状況証拠を得た。そこで本研究では、紋枯病菌が宿主を殺す前に宿主内に侵入していること、宿主免疫を撹乱するためのエフェクターと呼ばれる分泌タンパク質を使用していること、を明らかにすることで本菌の真の感染機構の解明を目指す。

Outline of Final Research Achievements

Rhizoctonia solani, a phytopathogenic fungus, infects a wide range of plant species and causes severe agricultural damage. To develop effective control measures, a thorough understanding of its infection strategies is essential. Our previous findings suggest that R. solani may undergo a biotrophic phase during its early infection process on leaves of monocotyledonous plants, in which it obtains nutrients from a living host. In this study, we found that this fungus employs small secreted proteins, known as effectors, that function to suppress host immunity. We also observed that R. solani can infect Arabidopsis thaliana, a model dicotyledonous plant, and that it induces resistance with phytohormones, suggesting that it infects necrotrophically, with minimal or no biotrophic interactions. Our results suggest that R. solani can discriminate between hosts and select different infection mechanisms as part of its infection strategy.

Academic Significance and Societal Importance of the Research Achievements

R. solaniは菌糸融合反応や培養菌叢から分類される様々な系統を含む種複合体だが、それら分類と病原性に相関がないことが、本研究からも示された。おそらく気温や土壌成分などの環境要因が適合した場所で増えた菌株が病気を引き起こしていると考えられる。ただし宿主や組織特異性も存在するため、それらは生理学的・ゲノム科学的な比較解析で明らかにする必要がある。少なくともイネなどでは活物寄生を行うことから、そこを標的とした防除法を検討する道が拓かれた。同じ菌株でも宿主により感染機構を使い分ける事実は本菌の感染機構の複雑性を示しており、その背景の解明は植物病理学・微生物学・進化学における学術的価値を有する。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • Research Products

    (21 results)

All 2023 2022 2021 Other

All Int'l Joint Research (3 results) Journal Article (5 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 4 results) Presentation (13 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results)

  • [Int'l Joint Research] Sichuan Agricultural University(中国)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] Sichuan Agricultural University(中国)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] Sichuan Agricultural University(中国)

    • Related Report
      2021 Annual Research Report
  • [Journal Article] Mitochondrial‐targeting effector RsIA_CtaG/Cox11 in <i>Rhizoctonia solani</i>?<scp>AG</scp>‐1 <scp>IA</scp> has two functions: plant immunity suppression and cell death induction mediated by a rice cytochrome <i>c</i> oxidase subunit2023

    • Author(s)
      Zhang Danhua、Lin Runmao、Yamamoto Naoki、Wang Zhaoyilin、Lin Hui、Okada Kazunori、Liu Yao、Xiang Xing、Zheng Tengda、Zheng Haoxi、Yi Xiaoqun、Noutoshi Yoshiteru、Zheng Aiping
    • Journal Title

      Molecular Plant Pathology

      Volume: 25 Issue: 1

    • DOI

      10.1111/mpp.13397

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Secreted Glycosyltransferase RsIA_GT of Rhizoctonia solani AG-1 IA Inhibits Defense Responses in Nicotiana benthamiana.2022

    • Author(s)
      Zhang D, Wang Z, Yamamoto N, Wang M, Yi X, Li P, Lin R, Nasimi Z, Okada K, Mochida K, Noutoshi Y, Zheng A.
    • Journal Title

      Pathogens

      Volume: 11(9) Issue: 9 Pages: 1026-1026

    • DOI

      10.3390/pathogens11091026

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Time-series transcriptome of Brachypodium distachyon during bacterial flagellin-induced pattern-triggered immunity2022

    • Author(s)
      Ogasahara Tsubasa, Kouzai Yusuke, Watanabe Megumi, Takahashi Akihiro, Takahagi Kotaro, Kim June-Sik, Matsui Hidenori, Yamamoto Mikihiro, Toyoda Kazuhiro, Ichinose Yuki, Mochida Keiichi, Noutoshi Yoshiteru
    • Journal Title

      Frontiers in Plant Science

      Volume: 13 Pages: 1004184-1004184

    • DOI

      10.3389/fpls.2022.1004184

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Surveillance of pathogenicity of Rhizoctonia solani Japanese isolates with varied anastomosis groups and subgroups on Arabidopsis thaliana2022

    • Author(s)
      Mai Mohsen Ahmed Abdelghany, Maria Kurikawa, Megumi Watanabe, Hidenori Matsui, Mikihiro Yamamoto, Yuki Ichinose, Kazuhiro Toyoda, Yusuke Kouzai, Yoshiteru Noutoshi
    • Journal Title

      Life

      Volume: 12 Issue: 1 Pages: 76-76

    • DOI

      10.3390/life12010076

    • NAID

      120007188353

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 植物の病害抵抗性を誘導する環状ペプチド2021

    • Author(s)
      能年義輝
    • Journal Title

      アグリバイオ

      Volume: 5 Pages: 412-416

    • Related Report
      2021 Annual Research Report
  • [Presentation] Small cyclic peptides that enhance disease resistance in Arabidopsis thaliana and Brachypodium distachyon2023

    • Author(s)
      Noutoshi, Y., Watanabe, M., Moriya, A., Kouzai, Y., Yamanaka, Y., Kimura, M., Abdelghany, M.M.A., Shoji, N., Kim, H., Saito, T., Kitamatsu, M.
    • Organizer
      The 33rd International Conference on Arabidopsis Research (ICAR2023)
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Virulence of Japanese isolates of Rhizoctonia solani on Brachypodium distachyon and barley in leaf and soil inoculation, and resistance induced by N-hydroxypipecolic acid in leaves2023

    • Author(s)
      Mahadevan, N., Kohno, N., Nagao, R., Nyein, K.T., Matsui, H., Yamamoto, M., Ichinose, Y., Toyoda, K., Kouzai, Y., Hisano, H., Okamoto, M., and Noutoshi, Y.
    • Organizer
      2023 IS-MPMI Congress
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Small cyclic peptides enhance resistance to diseases caused by Rhizoctonia solani in Arabidopsis thaliana and Brachypodium distachyon2023

    • Author(s)
      Noutoshi, Y., Watanabe, M., Moriya, A., Kouzai, Y., Yamanaka, Y., Kimura, M., Abdelghany, M.M.A., Shoji, N., Kim, H., Saito, T., Kitamatsu, M.
    • Organizer
      2023 IS-MPMI Congress
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 紋枯病菌のミナトカミジグサ葉への感染過程の菌糸の時系列観察2023

    • Author(s)
      山田悠生・前田直輝・渡邉恵・松井英譲・一瀬勇規・豊田和弘・能年義輝
    • Organizer
      令和5年度日本植物病理学会関西部会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 紋枯病菌のミナトカミジグサ葉への感染過程の菌糸の時系列観察2023

    • Author(s)
      山田悠生・前田直輝・渡邉恵・松井英譲・一瀬勇規・豊田和弘・能年義輝
    • Organizer
      令和5年度感染生理談話会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 群馬県のキャベツ病害から分離されたRhizoctonia sp. RhiCa-2株の種同定と防除法の検討2023

    • Author(s)
      本間大暉・添田萌子・渡邉恵・守屋綾子・松井英譲・一瀬勇規・豊田和弘・池田健太郎・能年義輝
    • Organizer
      令和6年度日本植物病理学会大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] ミナトカミジグサ葉上の紋枯病菌の時系列観察で見出された感染行動の役割2023

    • Author(s)
      山田悠生・前田直輝・渡邉恵・松井英譲・一瀬勇規・豊田和弘・能年義輝
    • Organizer
      令和6年度日本植物病理学会大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Infectivity of Rhizoctonia solani isolates on Brachypodium distachyon and barley, and chemical-induced resistance2023

    • Author(s)
      Mahadevan, N., Fernanda, R., Matsui, H., Ichinose, Y., Toyoda, K., Hisano, H., Kouzai, Y., Okamoto, M., Noutoshi, Y.
    • Organizer
      令和6年度日本植物病理学会大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] N-hydroxypipecolic acid confers resistance to Rhizoctonia solani in barley and Brachypodium distachyon.2023

    • Author(s)
      Mahadevan, N., Kohno, N., Nagao, R., Nyein, K.T., Matsui, H., Yamamoto, M., Ichinose, Y., Toyoda, K., Kouzai, Y., Hisano, H., Okamoto, M., Noutoshi, Y.
    • Organizer
      令和5年度日本植物病理学会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] The pathogenicity of Rhizoctonia solani Japanese isolates on Brachypodium distachyon and barley (Hordeum vulgare L. ssp. vulgare).2022

    • Author(s)
      Mahadevan, N., Kohno, N., Nagao, R., Nyein, K.T., Matsui, H., Yamamoto, M., Ichinose, Y., Toyoda, K., Hisano, H., Kouzai, Y., Noutoshi, Y.
    • Organizer
      Plant Microbe Research Network(PMRN) 第2回オンラインシンポジウム
    • Related Report
      2022 Annual Research Report
  • [Presentation] The pathogenicity of Rhizoctonia solani Japanese isolates on Brachypodium distachyon and barley (Hordeum vulgare L. ssp. vulgare).2022

    • Author(s)
      Mahadevan, N., Kohno, N., Nagao, R., Nyein, K.T., Matsui, H., Yamamoto, M., Ichinose, Y., Toyoda, K., Hisano, H., Kouzai, Y., Noutoshi, Y.
    • Organizer
      令和4年度日本植物病理学会関西部会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Infection assay of Rhizoctonia solani Japanese isolates to above- and below-ground tissues of Arabidopsis thaliana, Brachypodium distachyon, barley, and tomato.2022

    • Author(s)
      Mahadevan, N., Watanabe, M., Kohno, N., Nagao, R., Nyein, K.T., Matsui, H., Yamamoto, M., Ichinose, Y., Toyoda, K., Hisano, H., Kouzai, Y., Noutoshi, Y.
    • Organizer
      JSOL2022
    • Related Report
      2022 Annual Research Report
  • [Presentation] 抵抗性誘導効果を有する環状ペプチド2022

    • Author(s)
      渡邉恵, 守屋綾子, 香西雄介, 山中由理恵, 木村麻美子, Mai Mohsen Ahmed Abdelghany, 庄司直史, 金亨振, 斉藤太香雄, 北松瑞生, 能年義輝
    • Organizer
      日本農薬学会第47回大会シンポジウム「持続可能な食料システムに資する抵抗性誘導関連技術」
    • Related Report
      2021 Annual Research Report
    • Invited

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Published: 2021-04-28   Modified: 2025-01-30  

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