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Elucidation of rhizospheric consortium responses to two gradients of climate and soil acidity

Research Project

Project/Area Number 21H02232
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 40010:Forest science-related
Research InstitutionMie University

Principal Investigator

Matsuda Yosuke  三重大学, 生物資源学研究科, 教授 (30324552)

Co-Investigator(Kenkyū-buntansha) 谷川 東子  名古屋大学, 生命農学研究科, 准教授 (10353765)
北上 雄大  三重大学, 生物資源学研究科, 助教 (40882684)
小長谷 啓介  国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (90612739)
Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2023: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2022: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2021: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Keywordsスギ / 細根 / 真菌 / 細菌 / 線虫 / 窒素 / 人工林 / 細根系 / 真菌類 / 細菌類 / 内生菌 / アーバスキュラー菌根菌
Outline of Research at the Start

気候変動や山火事,土壌の酸性化などによる環境かく乱が,世界各地の森林の生態系機能の低下を引き起こしている.私たちは,「樹木根」,「細根に共生する菌根菌」,「根圏に生息する細菌」,「微生物を食する土壌動物(線虫)」を根圏コンソーシアム(共同体)と捉え,土壌酸性化が本州中部のスギ人工林の根圏コンソーシアムを変遷させることを明らかにした.次の段階として,気候変動に対する根圏コンソーシアムの応答,そのメンバーに加えて新規に発見された「機能が不明な根内内生菌」の理解をする必要が生じた.そこで本研究では,気候の変化が樹木の根圏微生物コンソーシアムの枠組みや働きに及ぼす影響を明らかにしようとしている.

Outline of Final Research Achievements

Environmental disturbances due to climate change and soil acidification are causing a decline in the functioning of forest ecosystems worldwide. In this study, tree roots, mycorrhizal fungi, bacteria and nematodes were considered as a rhizosphere consortium, and the aim was to clarify the effects of different climatic zones and soil acidification on this consortium. The rhizosphere fungal and bacterial communities and soil nematodes of cedar plantations in cool-temperate to subtropical regions were significantly affected by the soil environment inhabited. A 2.5-year incubation experiment using cedar roots and leaves as substrates showed that nitrate was the predominant nitrogen form in the root leachate, with a cumulative amount 22.6 times higher than in the leaf leachate. Bacterial communities were associated with nitrate concentration and leachate pH, whereas changes in fungal community structure were associated with leachate pH.

Academic Significance and Societal Importance of the Research Achievements

本研究は,スギ人工林における樹木細根を取り巻く土壌生物の動態を包括的に解き明かし,見えない地下部の生物多様性に関して有用な視点を提供した.さらに器官特異的なリターの窒素動態と関わる微生物を理解することで、窒素循環を予測するための視点を提供した.以上の情報は,局所的および地球規模の環境変化に対する森林生態系の応答,健全性を予測するための基礎情報を提供する.

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • Research Products

    (38 results)

All 2024 2023 2022 2021 Other

All Int'l Joint Research (2 results) Journal Article (11 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 11 results) Presentation (25 results) (of which Int'l Joint Research: 5 results,  Invited: 2 results)

  • [Int'l Joint Research] 台湾林業試験場(その他の国・地域)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] 台湾林業試験場(その他の国・地域)

    • Related Report
      2021 Annual Research Report
  • [Journal Article] Distribution and characterization of nematodes in above-ground microhabitats in a natural pristine cedar forest in Yakushima Island, Japan2024

    • Author(s)
      Kitagami Yudai、Matsuda Yosuke
    • Journal Title

      Canadian Journal of Zoology

      Volume: 102 Issue: 3 Pages: 264-271

    • DOI

      10.1139/cjz-2023-0107

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fungi and bacteria in the rhizosphere of Cryptomeria japonica exhibited different community assembly patterns at regional scales in East Asia2023

    • Author(s)
      Obase Keisuke、Kitagami Yudai、Tanikawa Toko、Chen Chien-Fan、Matsuda Yosuke
    • Journal Title

      Rhizosphere

      Volume: 28 Pages: 100807-100807

    • DOI

      10.1016/j.rhisph.2023.100807

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Contrasting patterns of nitrogen release from fine roots and leaves driven by microbial communities during decomposition2023

    • Author(s)
      Tanikawa Toko、Maie Nagamitsu、Fujii Saori、Sun Lijuan、Hirano Yasuhiro、Mizoguchi Takeo、Matsuda Yosuke
    • Journal Title

      Science of The Total Environment

      Volume: 855 Pages: 158809-158809

    • DOI

      10.1016/j.scitotenv.2022.158809

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Archaeal community structures associated with fine root systems of <i>Cryptomeria japonica</i> (Cupressaceae) in central Japan2022

    • Author(s)
      Mine Taichiro、Kitagami Yudai、Tanikawa Toko、Matsuda Yosuke
    • Journal Title

      Journal of Forest Research

      Volume: 28 Issue: 1 Pages: 33-41

    • DOI

      10.1080/13416979.2022.2108657

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High-throughput sequencing covers greater nematode diversity than conventional morphotyping on natural cedar forests in Yakushima Island, Japan2022

    • Author(s)
      Kitagami Yudai、Matsuda Yosuke
    • Journal Title

      European Journal of Soil Biology

      Volume: 112 Pages: 103432-103432

    • DOI

      10.1016/j.ejsobi.2022.103432

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of climatic and edaphic conditions on structuring patterns of soil nematode communities in Japanese cedar (Cryptomeria japonica) plantations2022

    • Author(s)
      Kitagami Yudai、Obase Keisuke、Chen Chien-Fan、Matsuda Yosuke
    • Journal Title

      Forest Ecology and Management

      Volume: 524 Pages: 120518-120518

    • DOI

      10.1016/j.foreco.2022.120518

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] High-throughput sequencing and conventional morphotyping show different soil nematode assemblages but similar community responses to altitudinal gradients on Mt. Ibuki, Japan2022

    • Author(s)
      Kitagami Yudai、Obase Keisuke、Matsuda Yosuke
    • Journal Title

      Pedobiologia

      Volume: 90 Pages: 150788-150788

    • DOI

      10.1016/j.pedobi.2021.150788

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of ectomycorrhizal fungal species on population growth and food preference of a fungivorous nematode2022

    • Author(s)
      Kitagami Yudai、Matsuda Yosuke
    • Journal Title

      Mycorrhiza

      Volume: 32 Issue: 1 Pages: 95-104

    • DOI

      10.1007/s00572-021-01063-0

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Soil spore bank communities of ectomycorrhizal fungi in Pseudotsuga japonica forests and neighboring plantations2022

    • Author(s)
      Okada Keita Henry、Matsuda Yosuke
    • Journal Title

      Mycorrhiza

      Volume: 32 Issue: 1 Pages: 83-93

    • DOI

      10.1007/s00572-021-01065-y

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spatial distribution of spore banks of ectomycorrhizal fungus, <i>Rhizopogon togasawarius</i>, at <i>Pseudotsuga japonica</i> forest boundaries2022

    • Author(s)
      Okada Keita Henry、Abe Hiroshi、Matsuda Yosuke、Nara Kazuhide
    • Journal Title

      Journal of Forest Research

      Volume: 印刷中 Issue: 4 Pages: 1-7

    • DOI

      10.1080/13416979.2021.2023386

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Colonization status and community structure of arbuscular mycorrhizal fungi in the coniferous tree, Cryptomeria japonica, with special reference to root orders2021

    • Author(s)
      Matsuda Yosuke、Kita Kohei、Kitagami Yudai、Tanikawa Toko
    • Journal Title

      Plant and Soil

      Volume: 468 Issue: 1-2 Pages: 423-438

    • DOI

      10.1007/s11104-021-05147-w

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Presentation] 針葉樹林と広葉樹林の土壌細菌・真菌・線虫群集構造と共起ネットワーク構造.2024

    • Author(s)
      北上雄大・松田陽介
    • Organizer
      第135回日本森林学会大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 樹木根圏に関わる微生物2024

    • Author(s)
      松田陽介
    • Organizer
      日本樹木医会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] 針葉樹人工林と広葉樹二次林の土壌と樹種の入れ替えが土壌線虫群集に与える影響2023

    • Author(s)
      北上雄大・松田陽介
    • Organizer
      第45回日本土壌動物学会大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 根内内生菌の種内変異が有機物分解能と植物の生育に及ぼす影響2023

    • Author(s)
      戸田やよい・大塚理香子・北上雄大・山中豪・松田陽介
    • Organizer
      第13回中部森林学会大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Proton and nitrogen releases from woody fine roots during their decomposition processes.2023

    • Author(s)
      Tanikawa T, Maie N, Fujii S, Sun L, Hirano Y, Mizoguchi T, Matsuda Y
    • Organizer
      10th International Conference on Acid Deposition
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effects of climatic and edaphic conditions on structuring patterns of soil nematode communities in Japanese cedar (Cryptomeria japonica) plantations2023

    • Author(s)
      Kitagami Y・Obase K・Chen CF・Matsuda Y
    • Organizer
      The 10th East Asian Federation of Ecological Societies International Congress
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Diversity and community structure of culturable root endophytic fungi isolated from Cryptomeria japonica grown at artificial forests along a latitudinal gradient2023

    • Author(s)
      Matsuda Y・Kitagami Y・Tanikawa T・Chen CF・Obase K
    • Organizer
      Asian Mycological Congress 2023
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 植物の根に関わる微生物~菌根菌の働きとその利用~.2023

    • Author(s)
      松田陽介
    • Organizer
      日本樹木医会三重県支部
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] スギ林の異なる基質が根圏におけるアンモニア酸化アーキア群集に与える影響2023

    • Author(s)
      大上楽・北上雄大・松田陽介
    • Organizer
      第134回日本森林学会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] スギ細根由来の内生菌のプテアーゼ活性とスギ実生の生育に及ぼす影響2023

    • Author(s)
      大塚理香子・北上雄大・山中豪・松田陽介
    • Organizer
      第134回日本森林学会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] スギ細根に由来する内生菌の接種がスギ実生の生育に及ぼす影響2022

    • Author(s)
      大塚理香子・北上雄大・山中豪・松田陽介
    • Organizer
      第12回中部森林学会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 真菌食性線虫の増殖に関わるスギ細根由来の内生菌の餌資源特性2022

    • Author(s)
      河村実紀・北上雄大・松田陽介
    • Organizer
      第12回中部森林学会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] スギ人工林の異なる基質がアンモニア酸化アーキアと細菌の分布に及ぼす影響2022

    • Author(s)
      大上楽・北上雄大・松田陽介
    • Organizer
      第12回中部森林学会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] スギ細根から分離された内生菌の成長量と植物体化学成分の分解能力2022

    • Author(s)
      千葉尚哉・松田陽介・平野恭弘・谷川東子
    • Organizer
      第12回中部森林学会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Genetic diversity of arbuscular mycorrhizal fungi associated with planted Japanese cedar trees along a latitudinal gradient2022

    • Author(s)
      Linda Y, Kitagami Y, Tanikawa T, Obase K, Chen CF, Matsuda Y
    • Organizer
      Eleventh International Conference on Mycorrhiza
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Community assemblages of rhizosphere bacteria and fungi along with environmental gradients in Cryptomeria japonica plantations, in Japan and Taiwan.2022

    • Author(s)
      Obase K, Mine T, Kitagami Y, Tanikawa T, Chen CF, Matsuda Y
    • Organizer
      Asian Mycological Congress 2021
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Diversity and assemblage patterns of soil nematodes in Cryptomeria japonica plantations.2022

    • Author(s)
      Kitagami Y, Obase K, Tanikawa T, Chen CF, Matsuda Y
    • Organizer
      第133回日本森林学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Community assembly of rhizosphere fungi and bacteria along environmental gradients in Cryptomeria japonica plantations2022

    • Author(s)
      Obase K, Mine T, Kitagami Y, Tanikawa T, Chen CF, Matsuda Y
    • Organizer
      第133回日本森林学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Culturable endophytic fungi of Cryptomeria japonica roots among man-made forests along a latitudinal gradient2022

    • Author(s)
      Matsuda Y, Kitagami Y, Tanikawa T, Chen CF, Obase K
    • Organizer
      第133回日本森林学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Species richness of arbuscular mycorrhizal fungal associated with Japanese cedar forest along a latitudinal gradient.2022

    • Author(s)
      Linda Y, Kitagami Y, Tanikawa T, Obase K, Chen CF, Matsuda Y
    • Organizer
      第133回日本森林学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 異なる生育環境がスギの細根形態とAM菌感染に及ぼす影響2022

    • Author(s)
      小島直也・Yustikasari Linda・北上雄大・松田陽介
    • Organizer
      第133回日本森林学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 外生菌根菌4種が真菌食性線虫Aphelenchoides sp.の個体数と餌選択性に及ぼす影響2021

    • Author(s)
      北上雄大・松田陽介
    • Organizer
      2021年度日本線虫学会 第28回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] スギ地上部の異なる器官に分布する線虫の群集構造と餌資源量の推定2021

    • Author(s)
      北上雄大・鈴木康平・松田陽介
    • Organizer
      第11回中部森林学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 異なる生育環境がスギ人工林における細根系の形態に及ぼす影響の解明2021

    • Author(s)
      小島直也・北上雄大・松田陽介
    • Organizer
      第11回中部森林学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 環境傾度に沿ったスギ根圏のアーキア群集構造2021

    • Author(s)
      峰太一郎・北上雄大・小長谷啓介・Chien-Fan Chen・松田陽介
    • Organizer
      生態学会2021年度中部地区大会
    • Related Report
      2021 Annual Research Report

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Published: 2021-04-28   Modified: 2025-01-30  

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