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Diazotrophic nitrogen fixation through aerial roots occurs in mangroves: implications for adaptation of mangrove plant growth to low-nitrogen tidal flats

Research Project

Project/Area Number 21H02239
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 40010:Forest science-related
Research InstitutionNational Institute for Environmental Studies

Principal Investigator

Inoue Tomomi  国立研究開発法人国立環境研究所, 生物多様性領域, 主幹研究員 (80435578)

Co-Investigator(Kenkyū-buntansha) 下野 綾子  東邦大学, 理学部, 准教授 (30401194)
高津 文人  国立研究開発法人国立環境研究所, 地域環境保全領域, 室長 (30514327)
Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2023: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2022: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2021: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Keywordsマングローブ / 通気組織 / 窒素固定 / 植物バクテリア相利共生 / マングローブ植物 / 窒素固定バクテリア / 呼吸根 / 相利共生
Outline of Research at the Start

課題代表者らは、マングローブ植物の地上根の通気組織を通じたガス輸送によって大気中の窒素ガスが土中の窒素固定バクテリアに供給されていることを明らかにした。このことは、マングローブ植物と窒素固定バクテリアとの間に相利共生関係が働いており、満潮時に土壌が冠水しても、通気組織が安定的な窒素供給経路として機能している可能性を示唆している。本研究では、マングローブ植物の根圏可給態窒素量が通気組織の発達の規定因子となっているかどうかを確かめる。以上によって、窒素不足になりやすい干潟土壌に生育するマングローブ植物の窒素獲得機構について、通気組織を介したガス輸送とバクテリア共生の側面から新たな知見を提供する。

Outline of Final Research Achievements

Mangrove trees maintain high levels of nitrogen fixation around their roots. The interior aerial space of mangrove roots, in which atmospheric gas is supplied through lenticels, could be efficient sites for nitrogen fixation. We measured tidal variations of partial pressure of N2 in root aerenchyma and conducted experiments using 15N2 as a tracer to track N2 movement through aerial roots of Avicennia marina.The nitrogenase activity and estimated nitrogen fixation through aerenchyma was higher in pneumatophores and absorbing roots than in cable roots. Positive correlations between root nitrogen contents and turnover rates of root nitrogen derived from N2 through aerenchyma suggested that the internal supply of N2 to diazotrophs could be the main source for nitrogen assimilation by roots. The aerial root structures could be an adaptation to survival in not only low-oxygen environments but also tidal flats with little plant-available nitrogen.

Academic Significance and Societal Importance of the Research Achievements

マングローブ植物に特徴的に見られる地上根の形態は、多くの植物学者の興味の対象である。本研究の成果は、窒素不足になりやすい干潟に生育するマングローブ植物の窒素獲得機構を明らかするだけではなく、マングローブ植物の地上根の構造がもつ進化適応の背景を理解することにも貢献する。さらに、温暖化による海水面変動や気温の季節性の変化といった、将来起こり得る環境変化によって大きな影響を受ける可能性が高い、マングローブ植物と土壌環境との関係について、植物の生理生態学的反応の理解を深めることに貢献する。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • Research Products

    (27 results)

All 2024 2023 2022 2021

All Journal Article (12 results) (of which Peer Reviewed: 12 results,  Open Access: 7 results) Presentation (15 results) (of which Int'l Joint Research: 4 results,  Invited: 3 results)

  • [Journal Article] Bruguiera gymnorhiza forms mycorrhizal associations but Rhizophora stylosa does not: A pot experiment using mangrove soil and Rhizophoraceae seedlings2024

    • Author(s)
      Akaji Yasuaki、Inoue Tomomi、Kohzu Ayato、Taniguchi Takeshi、Baba Shigeyuki
    • Journal Title

      Aquatic Botany

      Volume: 192 Pages: 103748-103748

    • DOI

      10.1016/j.aquabot.2023.103748

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diazotrophic nitrogen fixation through aerial roots occurs in <i>Avicennia marina</i>: implications for adaptation of mangrove plant growth to low‐nitrogen tidal flats2023

    • Author(s)
      Inoue Tomomi、Kohzu Ayato、Akaji Yasuaki、Miura Shingo、Baba Shigeyuki
    • Journal Title

      New Phytologist

      Volume: 241 Issue: 4 Pages: 1464-1475

    • DOI

      10.1111/nph.19442

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of soil temperature on the morphological and physiological traits of Kandelia obovata seedlings2023

    • Author(s)
      Akaji Yasuaki、Inoue Tomomi
    • Journal Title

      Plant Ecology

      Volume: 224 Issue: 6 Pages: 579-589

    • DOI

      10.1007/s11258-023-01325-1

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Temperature dependence of <scp> O <sub>2</sub> </scp> respiration in mangrove leaves and roots: implications for seedling dispersal phenology2022

    • Author(s)
      Inoue Tomomi、Akaji Yasuaki、Baba Shigeyuki、Noguchi Ko
    • Journal Title

      New Phytologist

      Volume: 237 Issue: 1 Pages: 100-112

    • DOI

      10.1111/nph.18513

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Relationship between plant growth and soil chemical properties in a mangrove afforestation stand, Kiribati2022

    • Author(s)
      Inoue Tomomi、Akaji Yasuaki、Kohzu Ayato、Hinokidani Ko、Adachi Hiroshi、Kezuka Mio、Baba Shigeyuki
    • Journal Title

      Plant and Soil

      Volume: 479 Issue: 1-2 Pages: 559-571

    • DOI

      10.1007/s11104-022-05545-8

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Temporal and interspecific dietary variation in wintering ducks in agricultural landscapes2022

    • Author(s)
      Ando Haruko、Ikeno Susumu、Narita Ayu、Komura Taketo、Takada Atsushi、Isagi Yuji、Oguma Hiroyuki、Inoue Tomomi、Takenaka Akio
    • Journal Title

      Molecular Ecology

      Volume: 00 Issue: 23 Pages: 1-13

    • DOI

      10.1111/mec.16584

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Parameterization of Mangrove Root Structure of Rhizophora stylosa in Coastal Hydrodynamic Model2022

    • Author(s)
      Mori Nobuhito、Chang Che-Wei、Inoue Tomomi、Akaji Yasuaki、Hinokidani Ko、Baba Shigeyuki、Takagi Masashi、Mori Sotaro、Koike Hironoshin、Miyauchi Miho、Suganuma Ryosuke、Sabunas Audrius、Miyashita Takuya、Shimura Tomoya
    • Journal Title

      Frontiers in Built Environment

      Volume: 7 Pages: 782219-782219

    • DOI

      10.3389/fbuil.2021.782219

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Distinct responses of growth and respiration to growth temperatures in two mangrove species2021

    • Author(s)
      Inoue Tomomi、Akaji Yasuaki、Noguchi Ko
    • Journal Title

      Annals of Botany

      Volume: 129 Issue: 1 Pages: 15-28

    • DOI

      10.1093/aob/mcab117

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Growth temperature affects O2 consumption rates and plasticity of respiratory flux to support shoot growth at various growth temperatures2021

    • Author(s)
      Inoue Tomomi、Yamada Yusuke、Noguchi Ko
    • Journal Title

      Plant, Cell &amp; Environment

      Volume: 45 Issue: 1 Pages: 133-146

    • DOI

      10.1111/pce.14217

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Arbuscular mycorrhizal fungal communities of a mangrove forest along a salinity gradient on Iriomote Island2021

    • Author(s)
      Akaji Yasuaki、Inoue Tomomi、Taniguchi Takeshi、Baba Shigeyuki
    • Journal Title

      Plant and Soil

      Volume: 472 Issue: 1-2 Pages: 145-159

    • DOI

      10.1007/s11104-021-05193-4

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Estimation of probable annual fine-root production and missing dead roots associated with the ingrowth core method: attempt with major mangrove species on Iriomote Island, southwestern Japan, locates in the subtropics2021

    • Author(s)
      Fujimoto K, Ono K, Watanabe S, Taniguchi S, Inoue T, Kanayama K, Ogawa T.
    • Journal Title

      Mangrove Science

      Volume: 12 Pages: 11-24

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Estimating the cost of coastal adaptation using mangrove forests against sea level rise2021

    • Author(s)
      Kumano Naoko、Tamura Makoto、Inoue Tomomi、Yokoki Hiromune
    • Journal Title

      Coastal Engineering Journal

      Volume: 63 Issue: 3 Pages: 263-274

    • DOI

      10.1080/21664250.2021.1892968

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Presentation] マングローブ植物の呼吸と成長の塩分に対する応答は生育温度によって異なる2024

    • Author(s)
      井上智美, 藤村朋子, 野口航
    • Organizer
      日本生態学会第71回全国大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 都市化に伴う植物の急速な進化に関する研究2024

    • Author(s)
      藤田知弘, 深野祐也, 小出大, 井上智美
    • Organizer
      日本生態学会第71回全国大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 生育温度と土壌養分条件がヤエヤマヒルギの成長とガス交換特性に与える影響2024

    • Author(s)
      河合清定, 赤路康朗, 井上智美, 小林正樹
    • Organizer
      日本生態学会第71回全国大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 大気二酸化炭素濃度と菌根共生がブナ実生の光合成能力に及ぼす影響2024

    • Author(s)
      赤路康朗, 鳥丸猛, 青日菜子, 山尾幸夫, 井上智美, 谷口武士
    • Organizer
      第135回日本森林学会大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Evolutionary consequences of urbanization on plant reproductive traits2023

    • Author(s)
      Fujita T., Koide D., Inoue T., Fukano Y.
    • Organizer
      BES Annual Meeting 2023
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The flower doesn’t flower in the city: Evolution of the plant reproductive traits in an urban population2023

    • Author(s)
      Fujita T., Koide D., Fukano.Y, Inoue T.
    • Organizer
      ESA 108th Annual meeting
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マングローブ植物の地上根通気組織と窒素固定2023

    • Author(s)
      井上智美、高津文人、赤路康朗、下野綾子
    • Organizer
      日本生態学会大会第70回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 都市環境における植物形質の進化2023

    • Author(s)
      藤田知弘、深野祐也、小出大、井上智美
    • Organizer
      日本生態学会大会第70回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 基礎研究と生物多様性保全:マングローブ生態系研究の魅力2023

    • Author(s)
      井上智美
    • Organizer
      日本生態学会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Carbon sequestration of mangroves at a global scale.2022

    • Author(s)
      Inoue T
    • Organizer
      International Conference on Blue Carbon Ecosystems for Sustainable Development with Special Emphasis to Mangrove Ecosystems
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] センシング分野の技術革新がもたらす新しいマングローブ研究2022

    • Author(s)
      宮城豊彦、井上智美、柳沢英明、渡辺信、馬場繁幸
    • Organizer
      第27回日本マングローブ学会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] マングローブ植物呼吸根の通気組織を介したガス輸送は低窒素環境への適応なのか?2021

    • Author(s)
      井上智美、高津文人、下野綾子
    • Organizer
      日本生態学会大会第68回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] マングローブ植物の窒素源としての硝酸態窒2021

    • Author(s)
      小山里奈、井上智美、松尾奈緒子
    • Organizer
      日本生態学会大会第68回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ニッチが異なるヒルギ科二種の菌根共生と細根形態様式2021

    • Author(s)
      赤路康朗、井上智美、谷口武士、馬場繁幸
    • Organizer
      日本生態学会大会第68回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Facilitation of soil nitrate production by mangrove roots in hypoxic soil condition.2021

    • Author(s)
      Morioka T, Koyama L, Inoue T, Kuroiwa M, Yokobe T, Tateno R.
    • Organizer
      AGU fall meeting
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research

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Published: 2021-04-28   Modified: 2025-01-30  

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