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Elucidation of microflora associated with fruits and vegetables during postharvest stage and its application for quality evaluation

Research Project

Project/Area Number 21H02320
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 41040:Agricultural environmental engineering and agricultural information engineering-related
Research InstitutionKagoshima University

Principal Investigator

Hamanaka Daisuke  鹿児島大学, 農水産獣医学域農学系, 准教授 (60399095)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2023: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2022: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2021: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Keywords農産物の保存 / 食の安全 / 品質保持 / 微生物群集 / 殺菌 / 収穫後農産物 / 微生物叢 / 品質評価 / 安全性 / 多様性 / 鮮度 / 品質 / 多様性解析 / 安全性確保 / 農産物保存
Outline of Research at the Start

本研究では、収穫後農産物の品質管理に関して、生きているが培養が困難である難培養微生物群を含む全ての微生物をターゲットとし、これらが形成する群集構造の全容解明と、その相互作用に影響を及ぼす細胞間ネットワーク情報伝達物質の同定、さらには品質と生理活性に影響を及ぼす微生物群集を多様性解析によって網羅系統的に把握し、影響要因評価に基づいた、真の品質保持期間を高精度に評価することを目的とする。既往手法である分析学的に示される品質や生理活性評価に加えて、微生物の動態を収穫後農産物のシェルフライフ評価のための新たなツールとして確立することを目指す。

Outline of Final Research Achievements

The dynamics of bacterial communities, including those of viable but non-culturable bacteria, co-existing in agricultural products during storage were examined. Changes in the quality and freshness of the produce and expression analysis of related genes were also examined, as well as damage to the physically stressed microorganisms and their growth characteristics after recovery. It was revealed that the genera and species of bacterial communities varied greatly even when the number of viable bacteria detected in the medium was the similar, depending on the storage environment of the produce. Furthermore, the recovery of microbial damage caused by physical stress was influenced by the presence of surfactants. Various polymer groups caused by such damage could be measured by near-infrared spectroscopy, suggesting the possibility of a simple and rapid understanding of the state of damage to microorganisms.

Academic Significance and Societal Importance of the Research Achievements

食品の保存性や微生物学的な安全性の精確な把握は、食の安全の確保ならびに食品ロスの低減にも大きく貢献するものである。本研究では、培地を用いた評価が困難な微生物群を含む全ての微生物を対象として、農産物の保存や流通中に遭遇すると予測される環境条件での精確な動態把握を試みたものである。これに加えて、ストレスを受けた場合に生じる損傷状態について、その増殖特徴について迅速な評価を試み、その可能性を示すことができた。これらの成果は、農産物の微生物学的安全性の精確な評価に加えて、保存性、すなわち市場商品価値の正確な把握において重要な基礎データとなることが期待できる。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • Research Products

    (34 results)

All 2024 2023 2022 2021

All Journal Article (8 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 8 results) Presentation (26 results) (of which Int'l Joint Research: 8 results,  Invited: 2 results)

  • [Journal Article] Effects of ultraviolet irradiation on the quality and bacterial diversity of fresh-cut cabbage during storage2024

    • Author(s)
      Sijie Zhang, Risa Kuramoto, Weiguo Wu, Daisuke Hamanaka
    • Journal Title

      Food Science and Technology Research

      Volume: 印刷中

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Effect of electric field treatment on postharvest quality of green bell pepper2024

    • Author(s)
      Yuko Hamaguchi, Riichiro Yoshida, Daisuke Hamanaka
    • Journal Title

      Acta Horticulturae

      Volume: 印刷中

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of High Hydrostatic Pressure Treatment Combined with Alkaline Electrolyzed Water on the Injury and Growth Characteristics of Bacterial Spores2022

    • Author(s)
      HAMANAKA DAISUKE、MORITA KOKI
    • Journal Title

      Biocontrol Science

      Volume: 27 Issue: 3 Pages: 131-138

    • DOI

      10.4265/bio.27.131

    • ISSN
      1342-4815, 1884-0205
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bacillus cereus芽胞の耐熱性と抗生物質感受性に及ぼす高圧とアルカリ性電解水の併用処理の影響2022

    • Author(s)
      濱中大介, 藤崎洋
    • Journal Title

      日本防菌防黴学会誌

      Volume: 50 Pages: 359-365

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Improving postharvest quality of tomato with the application of electric field treatment2022

    • Author(s)
      Siti Aisyah A.、Hamaguchi Y.、Yoshida R.、Hamanaka D.
    • Journal Title

      Acta Horticulturae

      Volume: 1336 Issue: 1336 Pages: 167-172

    • DOI

      10.17660/actahortic.2022.1336.22

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of the Combination of High Hydrostatic Pressure and Alkaline Electrolyzed Water on the Reduction of Heat Resistance of Bacterial Spores2021

    • Author(s)
      HAMANAKA DAISUKE、NAKA MASAKI、ARIMURA KYOHEI
    • Journal Title

      Biocontrol Science

      Volume: 26 Issue: 4 Pages: 193-199

    • DOI

      10.4265/bio.26.193

    • NAID

      130008140121

    • ISSN
      1342-4815, 1884-0205
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of electric field on the physiological activity and quality of cherry tomato fruit2021

    • Author(s)
      Ramnesh Ramneel Kishore, Hinako Masumoto, Riichiro Yoshida, Daisuke Hamanaka
    • Journal Title

      Journal of Kyushu Branch of the Japanese Society of Agricultural Machinery and Food Engineers

      Volume: 71 Pages: 16-20

    • NAID

      40022768575

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 保存条件の違いが細菌叢変化に及ぼす影響2021

    • Author(s)
      山崎晃介, 崎谷玲那, 鶴丸博人, 濱中大介
    • Journal Title

      九州農業食料工学会誌

      Volume: 71 Pages: 21-25

    • NAID

      40022767561

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Presentation] 近赤外分光法による細菌芽胞の耐熱性低下メカニズム評価の検討2023

    • Author(s)
      吉田知花, 有吉成志朗, 濱中大介
    • Organizer
      第77回九州農業食料工学会例会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 保存中のトマト果実の細菌叢に及ぼす紫外線照射の影響とその要因の評価2023

    • Author(s)
      鮫島陽佳, 藏元理彩, 濱中大介
    • Organizer
      第77回九州農業食料工学会例会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 保存中の非定常温度条件がレタス細菌叢の多様性変化に及ぼす影響2023

    • Author(s)
      藏元理彩, 濱中大介
    • Organizer
      農業環境工学関連学会2023合同大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 食品用乳化剤が高圧処理による細菌芽胞の耐熱性低下に及ぼす影響2023

    • Author(s)
      有吉成志朗, 濱中大介
    • Organizer
      農業環境工学関連学会2023合同大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] ピーマン果実の細胞壁強度に及ぼす電場環境の影響と定量PCRによる関連遺伝子群の発現解析2023

    • Author(s)
      濵口侑子, 吉田理一郎, 濱中大介
    • Organizer
      農業環境工学関連学会2023合同大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Effect of Electric Field Treatment on Postharvest Quality of Green Bell Pepper2023

    • Author(s)
      Yuko Hamaguchi, Riichiro Yoshida, Daisuke Hamanaka
    • Organizer
      The 4th Asian Horticultural Congress AHC2023
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effects of temperature fluctuation on microbial flora of cut lettuce2023

    • Author(s)
      Risa Kuramoto, Daisuke Hamanaka
    • Organizer
      The 4th Asian Horticultural Congress AHC2023
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 乳化剤の添加が高圧処理によるAlicyclobacillus acidoterrestris芽胞の耐熱性低下に及ぼす影響2023

    • Author(s)
      有吉成志朗, 濱中大介
    • Organizer
      日本防菌防黴学会第50回年次大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Electric fields: A potential technology for the shelf-life extension of fresh fruits and vegetables at postharvest stage2022

    • Author(s)
      Daisuke Hamanaka
    • Organizer
      International Conference on Agricultural and Agro-Industry-ICAAI2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Effect of the high hydrostatic pressure and food emulsifiers on the resistance of bacterial spores2022

    • Author(s)
      Masakaze Imamura, Taiga Kuhara, Daisuke Hamanaka
    • Organizer
      International Conference on Agricultural and Agro-Industry-ICAAI2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effect of the combination of high hydrostatic pressure and food emulsifiers on the reduction of heat resistance of bacterial spores2022

    • Author(s)
      Masakaze Imamura, Daisuke Hamanaka
    • Organizer
      21st World Congress of Food Science and Technology
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Application of high hydrostatic pressure processing under 100MPa for bacterial inactivation and shelf-life extension of food products2022

    • Author(s)
      Daisuke Hamanaka, Masakaze Imamura, Taiga Kuhara
    • Organizer
      21st World Congress of Food Science and Technology
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 乳化剤を用いた高圧処理がAllicyclobacillus acidoterrestris芽胞の耐熱性に及ぼす影響2022

    • Author(s)
      有吉成志朗, 今村仁風, 濱中大介
    • Organizer
      第76回九州農業食料工学会例会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 保存中の紫外線照射が農産物の品質に及ぼす影響2022

    • Author(s)
      藏元理彩, 濱中大介
    • Organizer
      第76回九州農業食料工学会例会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 細菌芽胞の水分と表面疎水性に及ぼすアルカリ性電解水と静水圧併用処理の影響2022

    • Author(s)
      濱中大介, 友松愛, 猪八重保志
    • Organizer
      日本防菌防黴学会第49回年次大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] カット青果物の細菌叢変化に及ぼす保存条件の影響2022

    • Author(s)
      濱中大介, 山﨑晃介
    • Organizer
      第80回農業食料工学会年次大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Influence of electric field treatment on quality attributes and microbial flora of tomato fruit stored at 20℃2022

    • Author(s)
      Abdullah Siti Aisyah, Daisuke Hamanaka
    • Organizer
      第80回農業食料工学会年次大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 乳化剤存在下における高圧処理が細菌芽胞の耐熱性低下に与える影響2022

    • Author(s)
      今村仁風, 久原大雅, 矢野大吉, 濱中大介
    • Organizer
      日本食品保蔵科学会第71回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 静水圧処理による食品の消費期限の延長効果2022

    • Author(s)
      濱中大介, 久原大雅, 今村仁風, 矢野大吉
    • Organizer
      日本食品保蔵科学会第71回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 洗浄殺菌が青果物の微生物叢に及ぼす影響2021

    • Author(s)
      小屋敷日菜子, 山崎晃介, 鶴丸博人, 濱中大介
    • Organizer
      第75回九州農業食料工学会例会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 乳化剤を用いた高圧処理がBacillus cereus芽胞の耐熱性に及ぼす影響2021

    • Author(s)
      矢野大吉, 今村仁風, 久原大雅, 濱中大介
    • Organizer
      第75回九州農業食料工学会例会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 異なる電場環境がトマト果実に及ぼす影響2021

    • Author(s)
      濵口侑子, 吉田理一郎, 濱中大介, Abdullah Siti Aisyah
    • Organizer
      第75回九州農業食料工学会例会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Improving postharvest quality of tomato with the application of electric field treatment2021

    • Author(s)
      Siti Aisyah Abdullah, Daisuke Hamanaka, Yuko Hamaguchi, Riichiro Yoshida
    • Organizer
      ISHS Asia Symposium on Quality Management in Postharvest Systems 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Application of electric field in food supply chain2021

    • Author(s)
      Daisuke Hamanaka
    • Organizer
      ISHS Asia Symposium on Quality Management in Postharvest Systems 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 青果物に与えるストレスが細菌叢の変化に及ぼす影響2021

    • Author(s)
      山崎晃介, 崎谷玲那, 鶴丸博人, 濱中大介
    • Organizer
      第79回農業食料工学会年次大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 食品用乳化剤添加が高圧処理による細菌芽胞の耐熱性低下に及ぼす影響2021

    • Author(s)
      今村仁風, 久原大雅, 濱中大介
    • Organizer
      第79回農業食料工学会年次大会
    • Related Report
      2021 Annual Research Report

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Published: 2021-04-28   Modified: 2025-01-30  

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