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Elucidating the pathophysiological factors for the clinical application using an artificial intelligence analysis to the snRNA transcriptome data of canine tumors

Research Project

Project/Area Number 21H02366
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 42020:Veterinary medical science-related
Research InstitutionKagoshima University

Principal Investigator

Miura Naoki  鹿児島大学, 農水産獣医学域獣医学系, 教授 (80508036)

Co-Investigator(Kenkyū-buntansha) 川原 幸一  大阪工業大学, 工学部, 教授 (10381170)
福島 隆治  東京農工大学, (連合)農学研究科(研究院), 教授 (10466922)
丸山 征郎  鹿児島大学, 医歯学総合研究科, 客員研究員 (20082282)
大和田 勇人  東京理科大学, 創域理工学部経営システム工学科, 教授 (30203954)
中川 貴之  東京大学, 大学院農学生命科学研究科(農学部), 准教授 (40447363)
畑井 仁  岩手大学, 農学部, 特任教授 (40566535)
後藤 章暢  兵庫医科大学, 医学部, 教授 (70283885)
Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2023: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2022: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2021: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Keywords犬 / 人工知能(AI) / ノンコーディングRNA / 腫瘍 / トランスクリプトーム / Y RNA / microRNA / sncRNA / AI / non-coding RNA / micro vesicles
Outline of Research at the Start

獣医療と人医療で問題である腫瘍病態に対して,新しい診断・治療ターゲットを選出し,病態への関与の解明と,実臨床応用を行う.犬の腫瘍組織,樹立腫瘍細胞株,さらに腫瘍分泌エクソソームの網羅的遺伝子発現(トランスクリプトーム)データをmRNAとnon-coding small RNAに分けて,腫瘍の病態解明を行う.この新規診断・治療ターゲットの発見解析に人工知能(AI)手法を導入する.さらに,新規ターゲットの機能解析を細胞株で行い,同時に臨床マーカーとしての有用性を調査する.

Outline of Final Research Achievements

This project aimed to select new tumor diagnostic and therapeutic targets and elucidate their involvement in pathogenesis. Transcriptional analysis of canine tumor tissues, established tumor cell lines, and tumor secretory endoplasmic reticulum has been done by NGS-RNA-Seq. Artificial intelligence (AI) methods were also introduced for target discovery analysis. Functional analysis of the novel targets was performed in cell lines. As a result, novel targets for microRNAs, which are problematic in many tumors, were identified. At the same time, we confirmed tumor-specific changes in Y RNA and tRNA fragments, which have not been reported in veterinary oncology. We also noted that microRNAs-450b and 301a contribute to tumor malignancy in a cell line model.

Academic Significance and Societal Importance of the Research Achievements

本申請課題では,犬の腫瘍で発現するノンコーディングRNAの網羅的解析を複数の犬の腫瘍で行い,複数の国際専門誌に発表した.特に,獣医腫瘍学では例のないY RNAやtRNAのフラグメントが腫瘍で発現が著しく発現が低下すること,血液中でも減少することを報告した.特にY RNAは人医療でもほとんど報告されていない.これらの新発見は今後の臨床的にも意義が高い.また,犬のメラノーマ細胞外小胞体のノンコーディングRNAの発現が,転移病巣と関連することを世界で初めて確認した.さらに,マイクロRNA-450bや301aが腫瘍の悪性化に寄与することを観察した.これらは今後の治療標的となる可能性がある.

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • Research Products

    (28 results)

All 2024 2023 2022 2021 Other

All Int'l Joint Research (5 results) Journal Article (10 results) (of which Int'l Joint Research: 8 results,  Peer Reviewed: 10 results,  Open Access: 5 results) Presentation (13 results) (of which Int'l Joint Research: 8 results,  Invited: 2 results)

  • [Int'l Joint Research] ベルリン自由大学(ドイツ)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] リトアニア保健科学大学(リトアニア)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] ベルリン自由大学(ドイツ)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] リトアニア保健科学大学(リトアニア)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] ベルリン自由大学(ドイツ)

    • Related Report
      2021 Annual Research Report
  • [Journal Article] YRNA and tRNA fragments can differentiate benign from malignant canine mammary gland tumors2024

    • Author(s)
      Hasan MD Nazmul、Rahman Md Mahfuzur、Husna Al Asmaul、Nozaki Nobuhiro、Yamato Osamu、Miura Naoki
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 691 Pages: 149336-149336

    • DOI

      10.1016/j.bbrc.2023.149336

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Hypoxia-related Y RNA fragments as a novel potential biomarker for distinguishing metastatic oral melanoma from non-metastatic oral melanoma in dogs2024

    • Author(s)
      Hasan MD Nazmul、Rahman MD Mahfuzur、Husna Al Asmaul、Kato Daiki、Nakagawa Takayuki、Arif Mohammad、Miura Naoki
    • Journal Title

      Veterinary Quarterly

      Volume: 44 Issue: 1 Pages: 1-8

    • DOI

      10.1080/01652176.2023.2300943

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Upregulation and functional roles of miR-450b in canine oral melanoma2024

    • Author(s)
      Hasan MD Nazmul、Rahman Md. Mahfuzur、Husna Al Asmaul、Arif Mohammad、Jasineviciute Indre、Kato Daiki、Nakagawa Takayuki、Miura Naoki
    • Journal Title

      Non-coding RNA Research

      Volume: 9 Issue: 2 Pages: 376-387

    • DOI

      10.1016/j.ncrna.2024.01.017

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Novel Y RNA-Derived Fragments Can Differentiate Canine Hepatocellular Carcinoma from Hepatocellular Adenoma2023

    • Author(s)
      Ushio Norio、Hasan Md Nazmul、Arif Mohammad、Miura Naoki
    • Journal Title

      Animals

      Volume: 13 Issue: 19 Pages: 3054-3054

    • DOI

      10.3390/ani13193054

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Elevated expression of miR-301a and its functional roles in canine oral melanoma2023

    • Author(s)
      Hasan MD Nazmul、Rahman Md. Mahfuzur、Husna Al Asmaul、Arif Mohammad、Iwanaga Tomoko、Tsukiyama‐Kohara Kyoko、Jasineviciute Indre、Kato Daiki、Nakagawa Takayuki、Miura Naoki
    • Journal Title

      Veterinary and Comparative Oncology

      Volume: 22 Issue: 1 Pages: 78-88

    • DOI

      10.1111/vco.12954

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] microRNAs Are Abundant and Stable in Platelet-Rich Fibrin and Other Autologous Blood Products of Canines2023

    • Author(s)
      Jasineviciute Indre、Hasan Md Nazmul、Grigas Juozas、Pautienius Arnoldas、Stankevicius Arunas、Zymantiene Judita、Miura Naoki
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 24 Issue: 1 Pages: 770-770

    • DOI

      10.3390/ijms24010770

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] NGS-identified miRNAs in Canine Mammary Gland Tumors Show Unexpected Expression Alterations in qPCR Analysis2022

    • Author(s)
      CHEN HUI-WEN、LAI YU-CHANG、RAHMAN MD MAHFUZUR、HUSNA AL ASMAUL、HASAN MD NAZMUL、HATAI HITOSHI、MIYOSHI NORIAKI、YAMATO OSAMU、MIURA NAOKI
    • Journal Title

      In Vivo

      Volume: 36 Issue: 4 Pages: 1628-1636

    • DOI

      10.21873/invivo.12873

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Micro RNA differential expression profile in canine mammary gland tumor by next generation sequencing2022

    • Author(s)
      Chen Hui-Wen、Lai Yu-Chang、Rahman Md Mahfuzur、Husna Al Asmaul、Hasan MD Nazmul、Miura Naoki
    • Journal Title

      Gene

      Volume: 818 Pages: 146237-146237

    • DOI

      10.1016/j.gene.2022.146237

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Long non‐coding RNA and transfer RNA‐derived small fragments in exosomes are potential biomarkers for canine oral melanoma2022

    • Author(s)
      Husna Al Asmaul、Rahman Md Mahfuzur、Chen Hui‐Wen、Hasan Md Nazmul、Nakagawa Takayuki、Miura Naoki
    • Journal Title

      Veterinary and Comparative Oncology

      Volume: in press Issue: 3 Pages: 653-663

    • DOI

      10.1111/vco.12818

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Identification of melanoma‐specific exosomal miRNAs as the potential biomarker for canine oral melanoma2021

    • Author(s)
      Husna Al Asmaul、Rahman Md Mahfuzur、Lai Yu‐Chang、Chen Hui‐Wen、Hasan Md Nazmul、Nakagawa Takayuki、Miura Naoki
    • Journal Title

      Pigment Cell & Melanoma Research

      Volume: 34 Issue: 6 Pages: 1062-1073

    • DOI

      10.1111/pcmr.13000

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Upregulation of miR-450b promotes cell proliferation, migration, and clonogenicity, inhibits apoptosis, and alters the expression of PAX9, BMP4, and MMP9 transcripts in canine oral melanoma cell lines2024

    • Author(s)
      MD Nazmul Hasan, Mohammad Arif, Daiki Kato, Takayuki Nakagawa, Naoki Miura
    • Organizer
      WVCC2024Tokyo
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Hypoxia-related Y RNA fragments as a novel potential biomarker for distinguishing metastatic oral melanoma from non-metastatic oral melanoma in dogs2024

    • Author(s)
      Mohammad Arif, MD Nazmul Hasan, Daiki Kato, Takayuki Nakagawa, Naoki Miura
    • Organizer
      WVCC2024Tokyo
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 炎症の惹起と遷延化メカニズムと関連疾患2024

    • Author(s)
      三浦直樹
    • Organizer
      獣医アトピー・アレルギー・免疫学会 第18回シンポジウム
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] Upregulation of mir-301a and its functional roles in canine oral melanoma2023

    • Author(s)
      MD Nazmul Hasan, Nobuhiro Nozaki, Mohammad Arif, Naoki Miura
    • Organizer
      SaSSOH 2023
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification of novel Y RNA and tRNA fragments as a potential biomarker for differentiating benign from malignant canine mammary gland tumors using next-generation sequencing2023

    • Author(s)
      Mohammad Arif, MD Nazmul Hasan, Nobuhiro Nozaki, Naoki Miura
    • Organizer
      SaSSOH 2023
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 退形成性乏突起膠細胞腫に対して手術を行った犬の3例2022

    • Author(s)
      杉山慶樹、杉山伸樹、三浦直樹
    • Organizer
      令和4年度日本小動物獣医学会(九州地区)
    • Related Report
      2022 Annual Research Report
  • [Presentation] 肋骨に発生した血管肉腫に対し、拡大外科切除と術後化学療法を行い長期生存している若齢犬の1例2022

    • Author(s)
      橋本砂輝、森田真央、島袋泰治、亀田景子、三浦直樹
    • Organizer
      令和4年度日本小動物獣医学会(九州地区)
    • Related Report
      2022 Annual Research Report
  • [Presentation] Differential expression microRNAs in canine hepatocellular carcinoma with some similarities to human equivalents2022

    • Author(s)
      三浦直樹、橋本砂輝
    • Organizer
      第81回日本癌学会学術総会
    • Related Report
      2022 Annual Research Report
  • [Presentation] The Bio-Functional Aspect of Non-Coding Small RNA for Canine Melanoma2022

    • Author(s)
      Naoki Miura
    • Organizer
      II. INTERNATIONAL VII. NATIONAL VETERINARY PHARMACOLOGY AND TOXICOLOGY CONGRESS
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Expression of MicroRNA-301a and its Functional Roles in two Canine Oral Melanoma Cell lines2022

    • Author(s)
      Md. Nazmul Hasan, Md Mahfuzur Rahman, Naoki Miura
    • Organizer
      The 6th, AJIVE
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 猫の鼻腔内リンパ腫20例における治療法と予後の検討2021

    • Author(s)
      橋本砂輝、森田真央、島袋泰治、亀田景子、猪狩和明、高橋義明、三浦直樹
    • Organizer
      令和3年度日本小動物獣医学会(九州地区)
    • Related Report
      2021 Annual Research Report
  • [Presentation] Long non-coding RNA derived small RNA fragments in canine mammary malignant tumor2021

    • Author(s)
      Md. Nazmul Hasan, Al Asmaul Husna, Md Mahfuzur Rahman, Hui-Wen Chen, Naoki Miura
    • Organizer
      SaSSOH 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Hypoxic non-coding RNA signature in canine oral melanoma2021

    • Author(s)
      Yasunori Hino, Md. Mahfuzur Rahman, Al Asmaul Husna, Hui-Wen Chen, Md. Nazmul Hasan, Takayuki Nakagawa and Naoki Miura
    • Organizer
      SaSSOH 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research

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Published: 2021-04-28   Modified: 2025-01-30  

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