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Comprehensive and precise classification and characterization of Coding/Non-coding RNA

Research Project

Project/Area Number 21H02404
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 43010:Molecular biology-related
Research InstitutionNagoya University (2023)
Kyushu University (2021-2022)

Principal Investigator

Matsumoto Akinobu  名古屋大学, 理学研究科, 教授 (60741519)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2023: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2022: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2021: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
KeywordsLong non-coding RNA / Coding RNA / 翻訳プロファイリング / 機械学習
Outline of Research at the Start

Long non-coding RNA(lncRNA)の定義は「タンパク質をコードしない200塩基以上のRNA」とされているが、われわれはこれまでに、一部のlncRNAがポリペプチドを翻訳することを明らかにしてきた。しかしこの発見により、どのようなRNAが翻訳され、どのようなRNAが翻訳されないのかということを規定する原理が不明であるという問題点が明らかとなった。そこで本課題では、翻訳プロファイリング法という新技術を駆使してcoding RNA/non-coding RNAの正確な分類を行い、さらに機械学習を用いてそれらの性質を解明することを目標とする。

Outline of Final Research Achievements

Having shown that a fraction of long non-coding RNAs (lncRNAs) translate polypeptides, we next set out to identify the true lncRNAs. Using various translation profiling methods, we were able to accurately classify coding/non-coding RNAs and identified a group of RNAs without 5'caps. Although these RNAs do not have a 5'cap structure, they were unexpectedly observed to interact with the ribosome, suggesting that they may be translated. We have constructed a reporter system to artificially express these RNAs and will be able to study the properties of these RNAs in detail.

Academic Significance and Societal Importance of the Research Achievements

新種のRNAを同定したことはRNAの研究領域において非常に意義が高い。またそれら新種のRNAを解析するための実験系を構築したことから、今後これらRNAの意義がさらに明らかになることが期待される。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • Research Products

    (4 results)

All 2023 2022 2021

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (2 results) (of which Invited: 1 results)

  • [Journal Article] Identification of unannotated coding sequences and their physiological functions2022

    • Author(s)
      Ichihara Kazuya、Nakayama Keiichi I、Matsumoto Akinobu
    • Journal Title

      The Journal of Biochemistry

      Volume: 173 Issue: 4 Pages: 237-242

    • DOI

      10.1093/jb/mvac064

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Combinatorial analysis of translation dynamics reveals eIF2 dependence of translation initiation at near-cognate codons2021

    • Author(s)
      Ichihara Kazuya、Matsumoto Akinobu、Nishida Hiroshi、Kito Yuki、Shimizu Hideyuki、Shichino Yuichi、Iwasaki Shintaro、Imami Koshi、Ishihama Yasushi、Nakayama Keiichi I
    • Journal Title

      Nucleic Acids Research

      Volume: 49 Issue: 13 Pages: 7298-7317

    • DOI

      10.1093/nar/gkab549

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 5’キャップを持たず安定的に存在するmRNAの機能解析2023

    • Author(s)
      市原知哉、平田実奈、野島孝之、中山敬一、松本有樹修
    • Organizer
      第46回日本分子生物学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Hidden ORFの同定と機能解析 およびその発現制御機構の解明2022

    • Author(s)
      松本 有樹修
    • Organizer
      タンパク質研究シンポジウム
    • Related Report
      2022 Annual Research Report
    • Invited

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Published: 2021-04-28   Modified: 2025-01-30  

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