| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2023: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2022: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2021: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Outline of Final Research Achievements |
With the aim of verifying a “rotational model” of the stator PomAB complex of the bacterial flagellar motor, we have achieved the generation of mutants with glass substrate-anchoring and gold nanoparticle probe-binding tags that are applicable to single-molecule measurement under an optical microscope. Furthermore, by introducing a cysteine mutation in the plug region of PomB, reversible activation of Na ion transport by the PomAB complex was achieved by a low molecular weight compound. We believe that these results are an important step toward understanding how the stator complex rotates the bacterial flagellum to generate propulsive force.
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