| Project/Area Number |
21H02490
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 44020:Developmental biology-related
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| Research Institution | Kumamoto University |
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Principal Investigator |
Sheng Guojun 熊本大学, 国際先端医学研究機構, 特別招聘教授 (20399439)
|
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| Co-Investigator(Kenkyū-buntansha) |
永井 宏樹 熊本大学, 国際先端医学研究機構, リサーチ・スペシャリスト (80772508)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2023: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2022: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2021: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Keywords | chicken / hemagioblast / hematopoiesis / vasculogenesis / ETV2 / NPAS4L / hemangioblast / EMT / Embryo |
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| Outline of Research at the Start |
EMT is an important morphogenetic process. Its mis-regulation is associated with cancers. EMT also plays a role in hematopoietic development and disease. In this project, we will use the avian model and investigate the role of partial EMT during primitive hematopoiesis. We will characterize partial epithelial and mesenchymal features of hemangioblasts from stages HH4-10; analyze functional involvement of two EMT transcription factors (Snai2, Zeb2); investigate cross-talk between EMT TFs and hemangioblast TFs in partial EMT regulation and promote blood/vessel specification via EMT modulation.
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| Outline of Final Research Achievements |
We show that ancestral amniotes contain both ETV2 and NPAS4L genes and that ETV2, although absent in avian genomes, is able to initiate endothelial differentiation program, but not hematopoietic differentiation program.
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| Academic Significance and Societal Importance of the Research Achievements |
We offer an evo-devo example of how critical master regulators of blood and
vessel development can still change and evolve. Socially, our work
highlighted the importance of domestic and international collaborations.
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