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The role of a nonpolar transport-type auxin in plant defense mechanism

Research Project

Project/Area Number 21H02501
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

Kasahara Hiroyuki  東京農工大学, (連合)農学研究科(研究院), 教授 (00342767)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2023: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2022: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2021: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Keywordsオーキシン / 植物ホルモン / 生体防御 / 生合成 / 不活性化 / 代謝 / フェニル酢酸 / スベリン / アブシシン酸 / 成長制御 / 生体防御機構 / インドール酢酸
Outline of Research at the Start

オーキシンは植物の成長や分化、環境応答の制御に関わる重要な植物ホルモンである。最近我々は、非極性移動型オーキシンのフェニル酢酸(PAA)をシロイヌナズナに処理すると、スベリン層の形成を促進したり、生体防御関連遺伝子の発現を誘導したりすることを見出した。これによりPAAが生体防御の調節に関与していることが示唆された。これを検証するにはPAA欠損変異体の解析が重要であるが、まだこの欠損変異体は得られていない。本研究では、シロイヌナズナのPAA生合成遺伝子を同定し、その欠損変異体のスベリン層やその形成に関連する遺伝子を調べることなどにより、PAAの生体防御における役割を明らかにする。

Outline of Final Research Achievements

The plant hormone auxin plays an important role in the regulation of plant growth and development. In this study, we aimed to identify the biosynthetic genes of phenylacetic acid (PAA), a nonpolar transport-type auxin, in Arabidopsis and elucidate the role of PAA in plant defense using its biosynthetic mutants. Through analysis of candidate genes for PAA biosynthesis, we obtained results suggesting that the aldehyde oxidase family contributes to PAA biosynthesis. Moreover, it was suggested that aromatic aldehyde synthase, which synthesizes phenylacetaldehyde from phenylalanine, is involved in PAA biosynthesis.

Academic Significance and Societal Importance of the Research Achievements

本研究は、ユニークな移動特性を持つ天然オーキシンのフェニル酢酸(PAA)が、シロイヌナズナにおいてフェニルアセトアルデヒドを経由して合成されている可能性を示した。このPAAは植物の生体防御に関与することがこれまでの研究で示されていることから、本研究の成果は植物の基本的な生体防御メカニズムの解明や、PAAの生合成酵素を標的とした新たな農薬の開発に繋がる可能性がある。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • Research Products

    (10 results)

All 2023 2022 Other

All Int'l Joint Research (2 results) Journal Article (3 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (5 results) (of which Int'l Joint Research: 4 results,  Invited: 2 results)

  • [Int'l Joint Research] Washington University in St. Louis/University of California San Diego(米国)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] Washington University in St. Louis/University of California San Diego(米国)

    • Related Report
      2022 Annual Research Report
  • [Journal Article] Genetic Interaction between Arabidopsis SUR2/CYP83B1 and GNOM Indicates the Importance of Stabilizing Local Auxin Accumulation in Lateral Root Initiation2023

    • Author(s)
      Chieko Goto, Akira Ikegami, Tatsuaki Goh, Kaisei Maruyama, Hiroyuki Kasahara, Yumiko Takebayashi, Yuji Kamiya, Koichi Toyokura, Yuki Kondo, Kimitsune Ishizaki, Tetsuro Mimura, Hidehiro Fukaki
    • Journal Title

      Plant and Cell Physiology

      Volume: 64 Issue: 10 Pages: 1178-1188

    • DOI

      10.1093/pcp/pcad084

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 4-Phenylbutyric acid promotes plant regeneration as an auxin by being converted to phenylacetic acid via an IBR3-independent pathway2022

    • Author(s)
      Iwase Akira、Takebayashi Arika、Aoi Yuki、Favero David S、Watanabe Shunsuke、Seo Mitsunori、Kasahara Hiroyuki、Sugimoto Keiko
    • Journal Title

      Plant Biotechnology

      Volume: 39 Issue: 1 Pages: 51-58

    • DOI

      10.5511/plantbiotechnology.21.1224b

    • NAID

      130008161269

    • ISSN
      1342-4580, 1347-6114
    • Year and Date
      2022-03-25
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Chemical inhibition of the auxin inactivation pathway uncovers the roles of metabolic turnover in auxin homeostasis2022

    • Author(s)
      Fukui K, Arai K, Tanaka Y, Aoi Y, Kukshal V, Jez JM, Kubes MF, Napier R, Zhao Y, Kasahara H, Hayashi KI.
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 119 Issue: 32

    • DOI

      10.1073/pnas.2206869119

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Metabolic regulation of two natural auxins in plants2023

    • Author(s)
      Kasahara Hiroyuki
    • Organizer
      Auxin and cytokinin in plant development 2023
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Metabolic regulation of two naturally occurring auxins in plants2023

    • Author(s)
      Kasahara Hiroyuki
    • Organizer
      The 24th International Conference on Plant Growth Substances (IPGSA)
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Distinct regulation of two natural auxins in seed formation and thermomorphogenesis2023

    • Author(s)
      Maruyama Kaisei, Hirai Shoko, Kasahara Hiroyuki
    • Organizer
      The 24th International Conference on Plant Growth Substances (IPGSA)
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Auxin homeostasis is coordinately regulated by GH3 and AUX12023

    • Author(s)
      Hayashi Ken-ichiro, Arai Kazushi, Watahiki Masaaki, Napier Richard, Zhao Yunde, Fukui Kosuke, Kasahara Hiroyuki
    • Organizer
      The 24th International Conference on Plant Growth Substances (IPGSA)
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 種子形成過程及び温度形態形成における2種の天然オーキシンの量的制御2023

    • Author(s)
      丸山海成、平井晶子、笠原博幸
    • Organizer
      植物科学調節学会第58回大会
    • Related Report
      2023 Annual Research Report

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Published: 2021-04-28   Modified: 2025-01-30  

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