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Molecular Basis Involved in the Formation of Plasmodesmata, Crucial Structures for Plant Multicellularity

Research Project

Project/Area Number 21H02516
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 44040:Morphology and anatomical structure-related
Research InstitutionHokkaido University

Principal Investigator

FUJITA Tomomichi  北海道大学, 理学研究院, 教授 (50322631)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2023: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2022: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Keywords植物細胞間コミュニケーション / アブシジン酸 / 原形質連絡密度 / 原形質連絡 / ストレプト植物 / 進化 / 化合物
Outline of Research at the Start

本研究では、(1)コケ植物において1次PD形成の分子基盤とその形成過程を明らかにする。(2)この分子基盤が被子植物や陸上植物の祖先群であるシャジクモ藻でも機能するのかを調べ、ストレプト植物全体におけるPD形成の分子基盤の普遍性を検討する。そしてPD形成と生物多細胞性の進化について分子レベルでの理解を前進させる。

Outline of Final Research Achievements

Plasmodesmata (PD) have long been known as essential structures for intercellular communication in plants. However, the molecular mechanisms underlying the formation of PD, as well as the regulation of their structure and density, remain largely unknown. In this study, we focused on the study that PD density is reduced by more than half in mutants of the moss Physcomitrium patens, which have lost multicellularity, and in brood cells induced by the plant hormone abscisic acid. We elucidated part of the molecular regulatory mechanism involved in this process. Additionally, we provided an insight whether this molecular basis is conserved across streptophytes, including both charophyte algae and land plants.

Academic Significance and Societal Importance of the Research Achievements

植物の細胞間コミュニケーションに不可欠な細胞壁にあるナノサイズの構造として原形質連絡(PD)の存在が19世紀後半に初めて提唱された。その後、1960年前後になりこの構造は電子顕微鏡で広く陸上植物等に存在することが確認されたが、PDがどのように形作られ、またその密度がどのように制御されるかなどに関わる分子制御基盤はまだ多くの点で解明されていなかった。本研究によりこの一端の仕組みを明らかにすることができた。したがって学術的意義は極めて高い。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • Research Products

    (17 results)

All 2024 2023 2022 Other

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Book (7 results) Remarks (2 results)

  • [Journal Article] ABA signaling converts stem cell fate by substantiating a tradeoff between cell polarity, growth and cell cycle progression and abiotic stress responses in the moss Physcomitrium patens2023

    • Author(s)
      Marcel Pascal Beier, Chiyo Jinno, Natsumi Noda, Kohei Nakamura, Sumio Sugano, Yutaka Suzuki, Tomomichi Fujita
    • Journal Title

      Frontiers in Plant Science

      Volume: 14 Pages: 1303195-1303195

    • DOI

      10.3389/fpls.2023.1303195

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Abscisic acid switches cell division modes of asymmetric cell division and symmetric cell division in stem cells of protonemal filaments in the moss <i>Physcomitrium patens</i>2022

    • Author(s)
      Hiroguchi, A., Nakamura, K. and Fujita, T.
    • Journal Title

      Plant Biotechnology

      Volume: 39 Issue: 1 Pages: 13-17

    • DOI

      10.5511/plantbiotechnology.22.0107a

    • ISSN
      1342-4580, 1347-6114
    • Year and Date
      2022-03-25
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Surface-localized glycoproteins act through class C ARFs to fine-tune gametophore initiation in Physcomitrium patens2022

    • Author(s)
      Teh Ooi Kock、Singh Prerna、Ren Junling、Huang Lin Tzu、Ariyarathne Menaka、Salamon Benjamin Prethiviraj、Wang Yu、Kotake Toshihisa、Fujita Tomomichi
    • Journal Title

      Development

      Volume: 149 Issue: 24

    • DOI

      10.1242/dev.200370

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Abscisic acid signaling controls density of primary plasmodesmata in the moss Physcomitrium patens2024

    • Author(s)
      Chiyo Jinno, Ken Fujisaki, Izumi Yotsui, Motoki Ouchi, Satoshi Naramoto, Daisuke Takezawa, Yoichi Sakata and Tomomichi Fujita
    • Organizer
      日本植物生理学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] アブシジン酸と細胞間コミュニケーション、細胞運命制御の出会い2023

    • Author(s)
      神野智世、楢本悟史、藤田知道
    • Organizer
      日本植物学会
    • Related Report
      2023 Annual Research Report 2022 Annual Research Report
    • Invited
  • [Presentation] Environmental fluctuation and regulation of intercellular communication in the moss, Physcomitrium patens2023

    • Author(s)
      Tomomichi Fujita, Munenori Kitagawa, Takumi Tomoi, Kensuke Kawade, Chiyo Jinno
    • Organizer
      The 33rd International Conference on Arabidopsis Research
    • Related Report
      2023 Annual Research Report 2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ヒメツリガネゴケにおけるアブシジン酸シグナル経路を介した原形質連絡の密度制御2023

    • Author(s)
      神野智世、楢本悟史、藤田知道
    • Organizer
      日本植物学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] アブシジン酸はヒメツリガネゴケにおいて原形質連絡の形成を抑制する2022

    • Author(s)
      神野智世、藤田知道
    • Organizer
      日本植物生理学会
    • Related Report
      2021 Annual Research Report
  • [Book] コケ分子生物学を用いたSDGsへの取り組み2023

    • Author(s)
      藤田知道
    • Total Pages
      94
    • Publisher
      北隆館
    • Related Report
      2023 Annual Research Report
  • [Book] 環境ストレスにより新生するコケ植物の幹細胞2022

    • Author(s)
      神野智世, 藤田知道
    • Total Pages
      92
    • Publisher
      北隆館
    • Related Report
      2022 Annual Research Report
  • [Book] コケ植物の幹細胞新生にみるアブシジン酸を用いたしなやかな生存戦略2022

    • Author(s)
      神野智世, 藤田知道
    • Total Pages
      66
    • Publisher
      ニューサイエンス社
    • Related Report
      2022 Annual Research Report
  • [Book] Tracking intercellular movement of fluorescent proteins in bryophytes2022

    • Author(s)
      Takumi Tomoi, Yoan Coudert, Tomomichi Fujita
    • Total Pages
      12
    • Publisher
      Humana
    • ISBN
      9781071621318
    • Related Report
      2022 Annual Research Report
  • [Book] Callose detection and quantification at plasmodesmata in bryophytes2022

    • Author(s)
      Arthur Muller, Tomomichi Fujita, Yoan Coudert
    • Total Pages
      11
    • Publisher
      Humana
    • ISBN
      9781071621318
    • Related Report
      2022 Annual Research Report
  • [Book] Plasmodesmata2022

    • Author(s)
      Takumi Tomoi, Yoan Coudert, Tomomichi Fujita
    • Publisher
      Springer Nature
    • ISBN
      9781071621318
    • Related Report
      2021 Annual Research Report
  • [Book] 月刊「細胞」2022年5月号2022

    • Author(s)
      神野 智世・藤田 知道
    • Total Pages
      66
    • Publisher
      ニュー・サイエンス社
    • Related Report
      2021 Annual Research Report
  • [Remarks] 百年の眠り 眠れる森の美女の教え

    • URL

      https://www.hokudai.ac.jp/news/2022/12/post-1140.html

    • Related Report
      2022 Annual Research Report
  • [Remarks]

    • URL

      https://www.sci.hokudai.ac.jp/PlantSUGOIne/

    • Related Report
      2021 Annual Research Report

URL: 

Published: 2021-04-28   Modified: 2025-01-30  

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