Design principles of neural circuits that regulate adaptive animal behaviors

• Project/Area Number: 21H02576
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 46010:Neuroscience-general-related
• Research Institution: The University of Tokyo
• Principal Investigator: Nose Akinao 東京大学, 大学院新領域創成科学研究科, 教授 (30260037)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Project Status: Completed (Fiscal Year 2023)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2023: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2022: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2021: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: ショウジョウバエ / 行動制御 / 神経回路発達 / 軸索誘導 / 行動進化 / Wntシグナル / 軸策誘導

Outline of Research at the Start

環境中からもたらされる様々な感覚情報を適切な行動の実行につなげることは脳神経系の最も基本的な機能のひとつである。本研究ではこれを可能とする神経回路の分子発生機構をショウジョウバエ幼虫をモデルとして探る。申請者はこれまでに幼虫の前進、後退運動の選択において決定的な役割を果たす司令ニューロンWaveを発見し、その軸索走行制御において決定的な役割を果たす候補分子としてWnt受容体Fz2を同定している。本申請研究では、Fz2によるWaveの軸索走行制御が感覚―運動回路の入出力パターン、行動、神経結合に与える影響を包括的に解析することにより、適応的な行動制御を可能とする神経回路の構築原理を解読する。

Outline of Final Research Achievements

Linking various sensory stimuli to the execution of appropriate behaviors is a fundamental function of the nervous system, yet the underlying molecular developmental mechanisms remain unknown. Here, we showed that command-like Wave neurons match their somatosensory receptive field to appropriate motor outputs by changing their axon extension along the A-P axis in segment-specific manner via the Wnt4/Fz2 axon guidance molecules.

Academic Significance and Societal Importance of the Research Achievements

動物の行動が遺伝子にどのようにして書き込まれているのかを明らかにすることは人類の基本的な問いのひとつである。本研究ではショウジョウバエをモデルとした研究により、その一端を初めて明らかにするものである。Wnt/Fzシグナルは種を越えて体の前後軸に沿った軸索誘導に関与することが知られていることから、本研究で明らかとなった分子機構はヒトも含め一般性をもつものと期待される。

Research Products

• [Int'l Joint Research] University of Cambridge/University of St Andrews(英国)
• [Int'l Joint Research] University of Cambridge/University of St Andrews(英国)
• [Int'l Joint Research] University of Cambridge/University of St Andrews(英国)
• [Journal Article] A vacuum-actuated soft robot inspired by Drosophila larvae to study kinetics of crawling behaviour (2023)
  • Author(s): Sun Xiyang、Nose Akinao、Kohsaka Hiroshi
  • Journal Title: PLOS ONE Volume: 18 Issue: 4 Pages: 1-18
  • DOI: 10.1371/journal.pone.0283316
  • Peer Reviewed / Open Access
• [Journal Article] Synchronous multi-segmental activity between metachronal waves controls locomotion speed in Drosophila larvae (2023)
  • Author(s): Liu Yingtao、Hasegawa Eri、Nose Akinao、Zwart Maarten F、Kohsaka Hiroshi
  • Journal Title: eLife Volume: 12
  • DOI: 10.7554/elife.83328
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Segment-specific axon guidance by Wnt/Fz signaling diversifies motor commands in Drosophila larvae (2023)
  • Author(s): Takagi Suguru、Takano Shiina、Hashimoto Yusaku、Morise Shu、Zeng Xiangsunze、Nose Akinao
  • Journal Title: bioRxiv Volume: -
  • DOI: 10.1101/2023.09.05.555126
  • Open Access
• [Journal Article] Electrophysiological Validation of Monosynaptic Connectivity between Premotor Interneurons and the aCC Motoneuron in the Drosophila Larval CNS (2022)
  • Author(s): Giachello Carlo N. G.、Hunter Iain、Pettini Tom、Coulson Bramwell、Knufer Athene、Cachero Sebastian、Winding Michael、Arzan Zarin Aref、Kohsaka Hiroshi、Fan Yuen Ngan、Nose Akinao、Landgraf Matthias、Baines Richard A.
  • Journal Title: The Journal of Neuroscience Volume: 42 Issue: 35 Pages: 6724-6738
  • DOI: 10.1523/jneurosci.2463-21.2022
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A neuromechanical model for Drosophila larval crawling based on physical measurements (2022)
  • Author(s): Sun Xiyang、Liu Yingtao、Liu Chang、Mayumi Koichi、Ito Kohzo、Nose Akinao、Kohsaka Hiroshi
  • Journal Title: BMC Biology Volume: 20 Issue: 1 Pages: 130-130
  • DOI: 10.1186/s12915-022-01336-w
  • Peer Reviewed / Open Access
• [Journal Article] Comparative analysis of high- and low-level deep learning approaches in microsatellite instability prediction (2022)
  • Author(s): Park Jeonghyuk、Chung Yul Ri、Nose Akinao
  • Journal Title: Scientific Reports Volume: 12 Issue: 1 Pages: 12218-12218
  • DOI: 10.1038/s41598-022-16283-3
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Extraction of bouton-like structures from neuropil calcium imaging data (2022)
  • Author(s): Fukumasu Kazushi、Nose Akinao、Kohsaka Hiroshi
  • Journal Title: Neural Networks Volume: 156 Pages: 218-238
  • DOI: 10.1016/j.neunet.2022.09.033
  • Peer Reviewed / Open Access
• [Presentation] Transient suppression of tonically active neurons gates motor output in Drosophila larval crawling (2024)
  • Author(s): Date Takahisa、Liu Yingtao、Yamaguchi Akihiro、Rui Wu、Paul McNulty、Marc Gershow、Maarten Zwart、Nose Akinao、Kohsaka Hiroshi
  • Organizer: Asia Pacific Drosophila Neurobiology Conference 3
  • Int'l Joint Research
• [Presentation] Identification of a novel interneuron that regulates forward wave propagation in Drosophila larvae (2024)
  • Author(s): Seki Takaki、Kohsaka Hiroshi、Nose Akinao
  • Organizer: Asia Pacific Drosophila Neurobiology Conference 3
  • Int'l Joint Research
• [Presentation] A tonically active neuron implicated in the maintenance of muscle relaxation in Drosophila larvae (2023)
  • Author(s): Date Takahisa、Liu Yingtao、Nose Akinao、Kohsaka Hiroshi
  • Organizer: Neurobiology of Drosophila 2023
  • Int'l Joint Research
• [Presentation] Emergence of oscillatory motor activities in developing Drosophila embryos (2023)
  • Author(s): Zeng XiangSunZe、Komanome Yuko、Seki Takaki、Kazama Hokuto、Nose Akinao
  • Organizer: The 56th Annual Meeting of the Japanese Society of Developmental Biologists
  • Int'l Joint Research / Invited
• [Presentation] Two perpendicular stripe-wise structures in the neuropil involved in generation of Drosophila larval crawling behaviors (2023)
  • Author(s): Fukumasu Kazushi、Nose Akinao、Kohsaka Hiroshi
  • Organizer: 第46 回日本神経科学大会
• [Presentation] Revealing behavior-specific neuronal populations in Drosophila larvae with a photoconvertible calcium sensor (2023)
  • Author(s): Date Takahisa、Nose Akinao、Kohsaka Hiroshi
  • Organizer: 第46 回日本神経科学大会
• [Presentation] Mechanisms of neural circuit reorganization that enable coordinated movement (2023)
  • Author(s): Seki Takaki、Komanome Yuko、 Zeng XiangSunZe、Kohsaka Hiroshi、Nose Akinao
  • Organizer: 第46 回日本神経科学大会
• [Presentation] Spatiotemporal structure of synapse population activity in the motor circuits for crawling (2023)
  • Author(s): Fukumasu Kazushi、Nose Akinao、Kohsaka Hiroshi
  • Organizer: 第75回日本細胞生物学会大会
  • Invited
• [Presentation] Comprehensive characterization of behavior specific neurons in fruit fly larvae with a photoconvertible calcium sensor (2023)
  • Author(s): Takahisa Date, Akinao Nose, Hiroshi Kohsaka
  • Organizer: Adaptive Circuit Census (ACC) International Symposium 2023
  • Int'l Joint Research
• [Presentation] Molecular and cellular mechanisms underlying the emergence of spontaneous patterned activity crucial for motor development in Drosophila (2022)
  • Author(s): XiangSunZe Zeng, Yuko Komanome, Hokto Kazama, Akinao Nose
  • Organizer: NEURO2022
  • Int'l Joint Research / Invited
• [Presentation] Functional development of neural circuits that regulate animal behaviors (2022)
  • Author(s): Akinao Nose
  • Organizer: The Neurophysics of Locomotion
  • Int'l Joint Research / Invited
• [Presentation] Synchronous multi-segmental activity controls the speed of axial locomotion by modulating the interval between peristaltic waves in Drosophila larvae (2022)
  • Author(s): Yingtao Liu, Akinao Nose, Maarten Frans Zwart, Hiroshi Kohsaka
  • Organizer: SfN Neuroscience meeting 2022
  • Int'l Joint Research
• [Presentation] Synchronous multi-segmental activity between metachronal waves controls the crawling speed in fly larvae (2022)
  • Author(s): Yingtao Liu, Akinao Nose, Maarten F Zwart, Hiroshi Kohsaka
  • Organizer: 第45 回日本分子生物学会年会(MBSJ2022)
  • Invited
• [Remarks] 能瀬研究室
  • URL: http://bio.phys.s.u-tokyo.ac.jp/