| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2023: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2022: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2021: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
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| Outline of Final Research Achievements |
In this study, we have examined the mechanisms of long-term potentiation (LTP) at rodent hippocampal mossy fiber synapses, in order to understand how the synaptic strengths are regulated in an activity-dependent manner. We have opto-genetically stimulated mossy fibers to induce LTP, and applied direct presynaptic patch clamp afterwards to elucidate presynaptic changes after LTP. Electrical properties such as action potential waveform and Ca currents were unchanged. Capacitance measurements showed that the release probability of synaptic vesicles was increased after the induction of LTP. Super-resolution microscopy has shown the accumulation of Munc13-1 and RIM1 at the active zones. Taken together, we have shown that the distribution of active zone scaffold proteins dynamically changed upon LTP, which might underlie the presynaptic plasticity at mossy fiber synapses.
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