Analysis of the mechanism of ncRNA-mediated carcinogenesis using the DICER1 syndrome model

• Project/Area Number: 21H02706
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 49020:Human pathology-related
• Research Institution: Tokyo Medical University
• Principal Investigator: Kuroda Masahiko 東京医科大学, 医学部, 主任教授 (80251304)
• Co-Investigator(Kenkyū-buntansha): 梅津 知宏 東京医科大学, 医学部, 講師 (40385547) ; 須藤 カツ子 東京医科大学, 医学部, 兼任講師 (50126091) ; 大野 慎一郎 東京医科大学, 医学部, 講師 (90513680)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Project Status: Completed (Fiscal Year 2023)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2023: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2022: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2021: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: Dicer1症候群 / miRNA / 肝間葉性過誤腫 / 一次繊毛 / PKD1 / Prkcsh / 胆管癌 / 一次線毛 / Docer1症候群 / 肝腫瘍 / DICER1症候群 / miR-5p / miR-122 / lncRNA / non-coding RNA / miRNA-5p / miRNA-122 / DICER1

Outline of Research at the Start

microRNA (miRNA) やlncRNAなどの、non-coding RNA (ncRNA) の異常は、がんをはじめとした様々な疾患の発生に深く関与する。これらのRNAを標的とする治療戦略は、次世代の医薬品として期待されている。しかし、単一のncRNAの解析は行われているものの、その制御異常と疾患のメカニズムは不明な点が多い。そこで、本研究は、DICER1症候群の発がんメカニズムを解明することで、ncRNAが関与する未知の腫瘍発生のメカニズムの解明を目指し、臨床応用につなげていく。

Outline of Final Research Achievements

DICER1 syndrome is a familial cancer susceptibility syndrome in which germline mutations in the DICER1 gene cause a variety of malignant and benign tumours. However, the mechanism of tumourigenesis caused by hot spot mutations in the DICER1 gene remains unclear. Therefore, in this study, we attempted to generate genetically engineered mice that reproduce the genotype of DICER1 syndrome patients in a liver-specific manner. As a result, the model mice developed bile duct hyperplasia with fibrosis similar to congenital liver fibrosis, cystic liver tumours similar to Caroli syndrome, intrahepatic cholangiocarcinoma and hepatocellular carcinoma. This mouse model provides a useful tool for elucidating the potential mechanisms of tumourigenesis induced by DICER1 mutants.

Academic Significance and Societal Importance of the Research Achievements

これまで、生理的なDICER1の機能解析は、DICER1ノックアウトマウスによって行われてきた。その結果、少しずつ成熟miRNAの生合成の分子メカニズムは明らかになってきた。しかしながら、腫瘍化や発がん機構におけるmiRNAの生合成の破綻は、未だに完全に解明されていない。興味深いことに、DICER1症候群モデルマウスでは、胆管過形成や嚢胞形成の原因として知られている胆管上皮の一次繊毛の形成異常が認められた。これらの結果は、DICER1変異が欠損した一次繊毛を誘導することによって嚢胞性肝腫瘍に寄与することを示している。本研究成果は、DICER1症候群の包括的な理解を得るために有用である。

Research Products

• [Int'l Joint Research] Children's Minnesota(米国)
• [Int'l Joint Research] University of Toronto(カナダ)
• [Journal Article] A Cerebral Embolism Caused by a Malignant Peripheral Nerve Sheath Tumor in a Patient with Neurofibromatosis Type 1 (2024)
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  • Journal Title: Internal Medicine Volume: 63 Issue: 22 Pages: 3087-3091
  • DOI: 10.2169/internalmedicine.2996-23
  • ISSN: 0918-2918, 1349-7235
  • Year and Date: 2024-11-15
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• [Journal Article] Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches (2024)
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  • Journal Title: Journal of Extracellular Vesicles Volume: 13 Issue: 2
  • DOI: 10.1002/jev2.12404
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• [Journal Article] Plasma extracellular vesicle microRNAs reflecting the therapeutic effect of the CBP/β-catenin inhibitor PRI-724 in patients with liver cirrhosis. (2024)
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• [Journal Article] Pediatric hypertrophic cardiomyopathy caused by a novel TNNI3 variant (2024)
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  • Journal Title: Human Genome Variation Volume: 11 Issue: 1 Pages: 1-4
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• [Journal Article] Efficacy of interstitial photodynamic therapy using talaporfin sodium and a semiconductor laser for a mouse allograft glioma model (2024)
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• [Journal Article] Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges (2023)
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• [Journal Article] Suppression of antitumor cytokine IL-24 by PRG4 and PAI-1 may promote myxoid liposarcoma cell survival. (2023)
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• [Journal Article] Recurrence prediction in clear cell renal cell carcinoma using machine learning of quantitative nuclear features (2023)
  • Author(s): Matsubara Shuya、Saito Akira、Tokuyama Naoto、Muraoka Ryu、Hashimoto Takeshi、Satake Naoya、Nagao Toshitaka、Kuroda Masahiko、Ohno Yoshio
  • Journal Title: Scientific Reports Volume: 13 Issue: 1 Pages: 11035-11035
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• [Journal Article] Characterization of circulating <scp>miRNAs</scp> in the treatment of primary liver tumors (2023)
  • Author(s): Umezu Tomohiro、Tanaka Shogo、Kubo Shoji、Enomoto Masaru、Tamori Akihiro、Ochiya Takahiro、Taguchi Y.‐H.、Kuroda Masahiko、Murakami Yoshiki
  • Journal Title: Cancer Reports Volume: 7 Issue: 2 Pages: 1-9
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• [Journal Article] Smooth muscle differentiation of coronary intima in autopsy tissues after sirolimus-eluting stent implantation (2023)
  • Author(s): Kurata Atsushi、Harada Yuichiro、Fujita Koji、Ohno Shin-ichiro、Takanashi Masakatsu、Yoshizawa Saeko、Nagashima Yoji、Nagao Toshitaka、Yamaguchi Junichi、Kuroda Masahiko
  • Journal Title: Cardiovascular Pathology Volume: 66 Pages: 107554-107554
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  • Peer Reviewed / Open Access
• [Journal Article] miR-146a-5p and miR-191-5p as novel diagnostic marker candidates for ovarian clear cell carcinoma. (2023)
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• [Journal Article] Hypoxia-induced paclitaxel resistance in cervical cancer modulated by miR-100 targeting of USP15 (2023)
  • Author(s): Nishi Hirotaka、Ono Masanori、Ohno Shinichiro、Yamanaka Zenta、Sasaki Toru、Ohyashiki Kazuma、Ohyashiki Junko H.、Kuroda Masahiko
  • Journal Title: Gynecologic Oncology Reports Volume: 45 Pages: 101138-101138
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• [Journal Article] MiR-34a induces myofibroblast differentiation from renal fibroblasts. (2023)
  • Author(s): Suguru Saito, Shin-ichiro Ohno, Yuichirou Harada, Yoshihiko Kanno & Masahiko Kuroda.
  • Journal Title: Clinical and Experimental Nephrology (Clin Exp Nephrol) Volume: 27 Issue: 5 Pages: 411-418
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• [Journal Article] Development of multiple AI pipeline that predict NAC response of breast cancer using H&E-stained tissues. (2023)
  • Author(s): Bin Shen, Akira Saito, Ai Ueda, Koji Fujita, Mikihiro Hashimoto, Masaharu Kobayashi, Aashiq H. Mirza, Hans Peter Graf, Eric Cosatto, Shoichi Hazama, Hiroaki Nagano, Toshitaka Nagao, Eiichi Sato, Esther Cheng, Syed AF Hoda, Takashi Ishikawa, Masahiko Kuroda.
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• [Journal Article] Protective effects of conditioned media of immortalized stem cells from human exfoliated deciduous teeth on pressure ulcer formation (2023)
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  • DOI: 10.3389/fimmu.2022.1010700
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• [Journal Article] Prevalence of Merkel Cell Polyomavirus in Primary Eyelid Merkel Cell Carcinomas and Association With Clinicopathological Features. (2023)
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  • Journal Title: American Journal of Ophthalmology Volume: 249 Pages: 49-56
  • DOI: 10.1016/j.ajo.2022.12.001
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• [Journal Article] Chemically oligomerizable TDP-43: a novel chemogenetic tool for studying the pathophysiology of amyotrophic lateral sclerosis (2022)
  • Author(s): Kanekura Kohsuke、Yamanaka Yoshiaki、Miyagi Tamami、Kuroda Masahiko
  • Journal Title: Neural Regeneration Research Volume: 17 Issue: 11 Pages: 2434-2434
  • DOI: 10.4103/1673-5374.335803
  • Peer Reviewed / Open Access
• [Journal Article] How can we interpret the relationship between liquid-liquid phase separation and amyotrophic lateral sclerosis? (2022)
  • Author(s): Kanekura Kohsuke、Kuroda Masahiko
  • Journal Title: Laboratory Investigation Volume: 102 Issue: 9 Pages: 912-918
  • DOI: 10.1038/s41374-022-00791-x
  • Peer Reviewed / Open Access
• [Journal Article] Nuclear microRNAs release paused Pol II via the DDX21-CDK9 complex (2022)
  • Author(s): Shin-Ichiro Ohno, Keiki Oikawa, Toshiaki Tsurui, Yuichirou Harada, Kana Ono, Mizumo Tateishi, Aashiq Mirza, Masakatsu Takanashi, Kosuke Kanekura, Kumiko Nagase, Yoshihisa Shimada, Yujin Kudo, Norihiko Ikeda, Takahiro Ochiya, Xiaozhong Wang , Masahiko Kuroda
  • Journal Title: Cell Reports Volume: 12;39(2) Issue: 2 Pages: 110673-110673
  • DOI: 10.1016/j.celrep.2022.110673
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The prevalence of Merkel cell polyomavirus in primary eyelid Merkel cell carcinomas and association with clinical and histopathological characteristics. (2022)
  • Author(s): 小松 紘之, 臼井 嘉彦, 後藤 浩, 助田 葵, 山本 善也, 長尾 俊孝, 大野 慎一郎, 黒田 雅彦.
  • Journal Title: 東京医科大学雑誌 Volume: 80 Pages: 139-139
• [Journal Article] サルコイドーシス患者の硝子体液を用いた網羅的miRNA解析による検討. (2022)
  • Author(s): 朝蔭 正樹, 臼井 嘉彦, 禰津 直也, 清水 広之, 山川 直之, 坪田 欣也, 高梨 正勝, 黒田 雅彦, 後藤 浩.
  • Journal Title: 日本眼科学会雑誌 Volume: 126 Pages: 209-209
• [Journal Article] Order controls disordered droplets: structure-function relationships in C9ORF72-derived poly(PR) (2022)
  • Author(s): Kanekura Kohsuke、Hayamizu Yuhei、Kuroda Masahiko
  • Journal Title: American Journal of Physiology-Cell Physiology Volume: 322 Issue: 2 Pages: C197-C204
  • DOI: 10.1152/ajpcell.00372.2021
  • Peer Reviewed / Open Access
• [Journal Article] SORT1/LAMP2-mediated extracellular vesicle secretion and cell adhesion are linked to lenalidomide resistance in multiple myeloma (2022)
  • Author(s): Yamamoto Tomofumi、Nakayama Jun、Yamamoto Yusuke、Kuroda Masahiko、Hattori Yutaka、Ochiya Takahiro
  • Journal Title: Blood Advances Volume: 6 Issue: 8 Pages: 2480-2495
  • DOI: 10.1182/bloodadvances.2021005772
  • Peer Reviewed / Open Access
• [Journal Article] Multiple cancer type classification by small RNA expression profiles with plasma samples from multiple facilities (2022)
  • Author(s): Suzuki K, Igata H, Abe M, Yamamoto Y; small RNA based cancer classification project.
  • Journal Title: Cancer Science Volume: - Issue: 6 Pages: 2144-2166
  • DOI: 10.1111/cas.15309
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• [Journal Article] Acerola exosome-like nanovesicles to systemically deliver nucleic acid medicine via oral administration. (2021)
  • Author(s): Umezu T, Takanashi M, Murakami Y, Ohno SI, Kanekura K, Sudo K, Nagamine K, Takeuchi S, Ochiya T, Kuroda M.
  • Journal Title: Molecular therapy. Methods & clinical development. Volume: 10;21 Pages: 199-208
  • DOI: 10.1016/j.omtm.2021.03.006
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• [Journal Article] Kana Miyahara, Naoharu Takano, Yumiko Yamada, Hiromi Kazama, Mayumi Tokuhisa, Hirotsugu Hino, Koji Fujita, Edward Barroga, Masaki Hiramoto, Hiroshi Handa, Masahiko Kuroda, Takashi Ishikawa & Keisuke Miyazawa BRCA1 degradation in response to mitochondrial damage in breast cancer cells (2021)
  • Author(s): Kana Miyahara, Naoharu Takano, Yumiko Yamada, Hiromi Kazama, Mayumi Tokuhisa, Hirotsugu Hino, Koji Fujita, Edward Barroga, Masaki Hiramoto, Hiroshi Handa, Masahiko Kuroda, Takashi Ishikawa, Keisuke Miyazawa
  • Journal Title: Scientific Reports Volume: 11 Issue: 1 Pages: 8735-8735
  • DOI: 10.1038/s41598-021-87698-7
  • Peer Reviewed / Open Access
• [Journal Article] A case report on death from acute bacterial cholangitis accompanied by von Meyenburg complexes (2021)
  • Author(s): Watanabe Noriko、Ohno Shin-ichiro、Sakuma Moe、Kuriwaki Mayo、Miura Mai、Kuroda Masahiko
  • Journal Title: Medicine Volume: 100 Issue: 15 Pages: e25526-e25526
  • DOI: 10.1097/md.0000000000025526
  • Peer Reviewed / Open Access
• [Journal Article] WAPL induces cervical intraepithelial neoplasia modulated with estrogen signaling without HPV E6/E7. Oncogene. (2021)
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  • Journal Title: Oncogene Volume: 40(21) Issue: 21 Pages: 3695-3706
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• [Journal Article] Cisplatin inducesdifferentiation in teratomas derived from pluripotent stem cells. (2021)
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• [Journal Article] Machine Learning Approach for Intraocular Disease Prediction Based on Aqueous Humor Immune Mediator Profiles (2021)
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  • Journal Title: Ophthalmology Volume: 128 Issue: 8 Pages: 1197-1208
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• [Journal Article] Fascin as a useful marker for cancer-associated fibroblasts in invasive lung adenocarcinoma (2021)
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• [Journal Article] Phase separation and toxicity of C9orf72 poly(PR) depends on alternate distribution of arginine. (2021)
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• [Journal Article] Establishment of chemically oligomerizable TAR DNA-binding protein-43 which mimics amyotrophic lateral sclerosis pathology in mammalian cells (2021)
  • Author(s): Yamanaka Yoshiaki、Miyagi Tamami、Harada Yuichiro、Kuroda Masahiko、Kanekura Kohsuke
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• [Journal Article] The membrane-linked adaptor FRS2β fashions a cytokine-rich inflammatory microenvironment that promotes breast cancer carcinogenesis (2021)
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  • Journal Title: Proceedings of the National Academy of Sciences Volume: 118 Issue: 43 Pages: 1-12
  • DOI: 10.1073/pnas.2103658118
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• [Journal Article] An improved macromolecular crowding sensor CRONOS for detection of crowding changes in membrane-less organelles under stressed conditions. (2021)
  • Author(s): Miyagi T, Yamanaka Y, Harada Y, Narumi S, Hayamizu Y, Kuroda M, Kanekura K.
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• [Journal Article] Prediction of non-muscle invasive bladder cancer recurrence using machine learning of quantitative nuclear features (2021)
  • Author(s): Tokuyama Naoto、Saito Akira、Muraoka Ryu、Matsubara Shuya、Hashimoto Takeshi、Satake Naoya、Matsubayashi Jun、Nagao Toshitaka、Mirza Aashiq H.、Graf Hans-Peter、Cosatto Eric、Wu Chin-Lee、Kuroda Masahiko、Ohno Yoshio
  • Journal Title: Modern Pathology Volume: 35 Issue: 4 Pages: 533-538
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  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Expression of L-type amino acid transporter 1 is a poor prognostic factor for Non-Hodgkin's lymphoma. (2021)
  • Author(s): Jigjidkhorloo N, Kanekura K, Matsubayashi J, Akahane D, Fujita K, Oikawa K, Kurata A, Takanashi M, Endou H, Nagao T, Gotoh A, Norov O, Kuroda M.
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  • DOI: 10.1038/s41598-021-00811-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Edible plant-derived nanovesicles to systemic deliver nucleic acid medicine for oral administration (2024)
  • Author(s): Tomohiro Umezu, Mamoru Yanagimachi, Masakatsu Takanashi, Yoshiki Murakami, Masahiko Kuroda.
  • Organizer: ISEV2024（第13回国際細胞外小胞学会）
  • Int'l Joint Research
• [Presentation] 子宮頸部液状細胞診のAIによる自動分類システムの開発 (2024)
  • Author(s): 堀内 啓、齋藤 彰、沈 彬、黒田 雅彦
  • Organizer: 第113回日本病理学会総会
• [Presentation] 肝臓特異的DICER1症候群モデルマウスは一次繊毛の形態異常を伴う嚢胞性肝腫瘍を発症する (2024)
  • Author(s): 大野慎一郎、原田裕一郎、黒田雅彦
  • Organizer: 第113回日本病理学会総会
• [Presentation] Trousseau症候群剖検例における血栓形成機序の免疫組織学的解析 (2024)
  • Author(s): 山田 侑子、倉田 厚、藤田 浩司、高橋達彦、高木央、荻原優衣、高江洲央、黒田雅彦
  • Organizer: 第113回 日本病 理学会総会
• [Presentation] 髄芽腫細胞株でのYM155と細胞分化促進剤の相乗効果および 病理検体でのアポトーシス関連蛋白発現の検討 (2024)
  • Author(s): 永松由衣、石倉礼央、齋藤峻、田中桐悟、味元寛貴、渡辺凜汰朗、福岡講平、中澤温子、渡辺紀子、黒田雅彦
  • Organizer: 第113回日本病理学会総会
• [Presentation] AIによるがん治療後の再発予測‐腫瘍形態学におけるAIの応用‐ (2024)
  • Author(s): 黒田 雅彦
  • Organizer: 第101回日本病理学会関東支部学術集会
  • Invited
• [Presentation] がんにおけるパッセンジャー変異の意義と臨床応用 (2024)
  • Author(s): 黒田 雅彦、梅津知宏
  • Organizer: 第8回Liquid Biopsy研究会
  • Invited
• [Presentation] Case Series of Pediatric Cardiomyopathy with Both Autopsy and Postmortem Genetic Testing: A Utility of Postmortem Genetic Testing for Pediatric Autopsy. (2024)
  • Author(s): Noriko Watanabe, Kayoko Ichimura, Atsuko Nakazawa, Kenji Hoshino, Daiju Oba, Hirofumi Ohashi, Koji Fujita, Masahiko Kuroda
  • Organizer: USCAP (United States and Canadian Academy of Pathology) 113th Annual Meeting
  • Int'l Joint Research
• [Presentation] ハスカップ果実抽出物の原発性胆汁性胆管炎モデルマウスに対する効果 (2024)
  • Author(s): ノミン トール・アリウンボルド、エルデネツォグト・ドゥングバト、梅村昌宏、川田一仁、沈 彬、黒田雅彦、有働恵美子、古里文吾、福里利夫、高橋芳久
  • Organizer: 第113回日本病理学会総会
• [Presentation] Abcb4-/-マウスの肝病変の経時的変化とそれに対するハスカップ果実抽出物の効果 (2024)
  • Author(s): アマルサナー・ナランバヤル、エルデネツォグト・ドゥングバト、草野広行、沈 彬、黒田雅彦、早田正和、古里文吾、福里利夫、高橋芳久
  • Organizer: 第113回日本病理学会総会
• [Presentation] 新しい老化の概念 -ゲノムからみた老化現象とその予防- (2023)
  • Author(s): 黒田 雅彦
  • Organizer: 高齢者の生活習慣病マネジメント講演会
  • Invited
• [Presentation] 植物由来エクソソーム様小胞を担体とした経口RNA医薬品の開発 (2023)
  • Author(s): 黒田 雅彦
  • Organizer: 第10回日本細胞外小胞学会学術集会
  • Invited
• [Presentation] Adventitial collagen fibrils in mouse model for Vascular Ehlers-Danlos syndrome (2023)
  • Author(s): Kazuyo Kiribayashi, Shinichiro Ohno, Yuichiro Harada, Natsuko Inagaki, Masahiko Kuroda
  • Organizer: Human Genetics Asia 2023
  • Int'l Joint Research
• [Presentation] ファブリー病におけるGb3の細胞内動態と ERストレスについての検討 (2023)
  • Author(s): 桐林和代, 梅津知宏, 稲垣夏子, 黒田雅彦
  • Organizer: 第47回日本遺伝カウンセリング学会学術集会
• [Presentation] 肝臓特異的DICER1症候群モデルマウスは先天性肝線維症、カロリ症候群、肝内胆管癌を発症する (2023)
  • Author(s): 大野慎一郎、原田裕一郎、黒田雅彦
  • Organizer: 第82回日本癌学会学術総会
• [Presentation] HCVをクリアランスしたのちに発癌した症例の末梢血miRNA解析 (2023)
  • Author(s): 村上 善基、梅津 知宏、田中 肖吾、久保 正二、榎本 大、田守 昭博、落谷 孝広、田口 善弘、黒田 雅彦
  • Organizer: 第82回日本癌学会学術総会
• [Presentation] 肺癌患者由来cfDNAから検出されたde novo mutational signatureの同定と生物学的意義の解明 (2023)
  • Author(s): 梅津 知宏、工藤 勇人、嶋田 義久、大平 達夫、村上 善基、池田 徳彦、黒田 雅彦
  • Organizer: 第82回日本癌学会学術総会
• [Presentation] キッドバイオプシーを用いたがんゲノム医療の新しい展開 －パッセンジャー異変の意義と臨床応用－ (2023)
  • Author(s): 黒田 雅彦
  • Organizer: 第131回東京脳腫瘍研究会
  • Invited
• [Presentation] AIによるがん治療後の再発予測 －腫瘍形態学におけるAIの応用－ (2023)
  • Author(s): 黒田 雅彦
  • Organizer: 第56回日本整形外科学会 骨・軟部腫瘍学術集会
• [Presentation] Edible plant-derived nanovesicles to systemic deliver nucleic acid medicine for oral administration (2023)
  • Author(s): Tomohiro Umezu, Mamoru Yanagimachi, Masakatsu Takanashi, Yoshiki Murakami, Kenichi Nagamine, Shin Takeuchi, Masahiko Kuroda
  • Organizer: ASGCT 26th Annual meeting
  • Int'l Joint Research
• [Presentation] 肝臓特異的DICER1症候群モデルマウスは嚢胞性肝腫瘍を発症する (2023)
  • Author(s): 大野慎一郎、原田裕一郎、黒田雅彦
  • Organizer: 第112回日本病理学会総会
• [Presentation] 大腸癌予後に関与するMutation Signatureの解析 (2023)
  • Author(s): 梅津知宏、齋藤彰、黒田雅彦
  • Organizer: 第112回日本病理学会総会
• [Presentation] 理解剖例における網羅的遺伝子検索を目指した核酸品質の検討-追加報告- (2023)
  • Author(s): 渡辺紀子、市村香代子、村上仁彦、中澤温子、梅津知宏、黒田雅彦
  • Organizer: 第112回日本病理学会総会
• [Presentation] 人工知能パイプラインによる乳癌の NAC 療法の効果予測モデルの開発. (2023)
  • Author(s): 沈彬、齋藤彰、黒田雅彦
  • Organizer: 第112回日本病理学会総会
• [Presentation] 剖検によりG-CSF産生肺多形癌と診断した原発不明がんの一例 (2023)
  • Author(s): 山田侑子、赤木舞、藤田浩司、助田葵、荒井勇弥、若松昇平、吉澤成一郎、長尾俊孝、黒田雅彦
  • Organizer: 第112回日本病理学会総会
• [Presentation] Structural decoding of C9ORF72-poly(PR) (2022)
  • Author(s): 金蔵孝介、宮城碧水、黒田雅彦
  • Organizer: 第41回日本認知症学会
• [Presentation] A novel molecular mechanism for inhibition of anti-tumor cytokine IL-24 expression in myxoid liposarcoma cells (2022)
  • Author(s): Kosuke Oikawa, Fuyuki Sato1, Masahiko Kuroda, Shogo Ehata
  • Organizer: 第81回日本癌学会学術総会
• [Presentation] A mouse model of DICER1 syndrome develops cirrhosis, bile duct hyperplasia and hepatic tumors (2022)
  • Author(s): Shinichiro Ohno, Yuichirou Harada, Masahiko Kuroda
  • Organizer: 第81回日本癌学会学術総会
• [Presentation] Integration of AI-based histopathology and genomic mutation analysis (2022)
  • Author(s): Akira Saito, Tomohiro Umezu, Masahiko Kuroda
  • Organizer: 第81回日本癌学会学術総会
• [Presentation] Identification of novel mutational signature of precancerous lesions by liquid biopsy using cell-free DNA. (2022)
  • Author(s): Tomohiro Umez, Ryohei Kudo, Yoshiki Murakami, Shinichiro Ohno, Yujin Kudo, Norihiko Ikeda, Masahiko Kuroda
  • Organizer: 第81回日本癌学会学術総会
• [Presentation] Suprression of the canonical TGF-βsignaling via SMAD3/4 renders EGFR-mutant lung sdenocarcinoma resistant to EGFR-TKIs. (2022)
  • Author(s): Eunjin Bae, Jeongwan Yoon, Yasuo Nagafuchi, Yojiro Makino, Atsumi Tamura, Inkyu Lee, Jinsoo Han, Jihyun Ju, Aoi Sukeda, Toshitaka Nagao, Mitsuyasu Kato, Keishi Fujio, Masahiko Kuroda, Tatsuo Ohira, Notihiko Ikeda, Mizuko Mamura
  • Organizer: 第81回日本癌学会学術総会
• [Presentation] Alternating Arg distribution controls phase separation and toxicity of poly(PR). (2022)
  • Author(s): Kohsuke Kanekura, Yuhei Hayamizu, Masahiko Kuroda.
  • Organizer: Neuro2022
• [Presentation] 経口投与によるアセロラ果汁由来小胞のDDS担体としての有用性 (2021)
  • Author(s): 梅津知宏、髙梨正勝、大野慎一郎、原田裕一郎、黒田雅彦
  • Organizer: 第8回日本細胞外小胞学会 (JSEV)
• [Presentation] 樹状細胞由来エクソソームは内包因子をT細胞に伝達することで免疫系を活性化する (2021)
  • Author(s): 髙梨正勝、須藤カツ子、上田しのぶ、黒田雅彦
  • Organizer: 第8回日本細胞外小胞学会 (JSEV)
• [Presentation] 腫瘍形態学におけるAIの応用 (2021)
  • Author(s): 黒田 雅彦
  • Organizer: 第38回日本眼腫瘍学会
  • Invited
• [Presentation] Cisplatin induces differentiation in pluripotent stem cell-derived immature teratoma (2021)
  • Author(s): Atsushi Kurata, Masakatsu Takanashi, Shin-ichiro Ohno, Masahiko Kuroda
  • Organizer: 第80回日本癌学会学術総会
• [Presentation] Potent ALK5 inhibition facilitates progression and metastasis of mouse melanoma (2021)
  • Author(s): Jeong-Hwan Yoon, Eunjin Bae, Inkyu Lee, Jin Soo Han, Ji-Hyun Ju, Masahiko Kuroda, and Mizuko Mamura
  • Organizer: 第80回日本癌学会学術総会
• [Presentation] Canonical TGF-β signaling via SMAD3 and SMAD4 suppresses Th1 and Th17 differentiation in psoriasis (2021)
  • Author(s): Jeong-Hwan Yoon, Eunjin Bae, Inkyu Lee, Jin Soo Han, Ji-Hyun Ju, Masahiko Kuroda, Yukari Okubo and Mizuko Mamura. Canonical TGF-β signaling via SMAD3 and SMAD4 suppresses Th1 and Th17 differentiation in psoriasis
  • Organizer: 第85回日本インターフェロン・サイトカイン学会学術集会
• [Presentation] Linker-phosphorylated SMAD2 induces resistance to tyrosine kinase inhibition in EGFR-mutated lung adenocarcinoma. (2021)
  • Author(s): Eunjin Bae Jeong-Hwan Yoon, Inkyu Lee, Jin Soo Han, Ji-Hyun Ju, Aoi Sukeda, Toshitaka Nagao, Tatsuo Ohira, Norihiko Ikeda, Masahiko Kuroda and Mizuko Mamura
  • Organizer: 第85回日本インターフェロン・サイトカイン学会学術集会
• [Presentation] Model mice that mimic the genotype of DICER1 syndrome develop hepatic mesenchymal hamartoma. (2021)
  • Author(s): 大野慎一郎、原田裕一郞、黒田雅彦
  • Organizer: 第110回日本病理学会総会
• [Presentation] シスプラチンによるマウス多能性幹細胞由来未熟奇形腫の分化誘導 (2021)
  • Author(s): 倉田 厚、高梨 正勝、藤田 浩司、黒田 雅彦.
  • Organizer: 第110回日本病理学会総会
• [Presentation] 肝膿瘍の剖検FFPE検体から16S rDNAシークエンスにて緑膿菌を同定しえた一例 (2021)
  • Author(s): 佐久間萌、栗脇真世、三浦舞、渡辺紀子、黒田雅彦
  • Organizer: 第110回 日本病理学会総会