遺伝子改変技術によるロタウイルス感染制御基盤の確立

• Project/Area Number: 21H02739
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 49060:Virology-related
• Research Institution: Osaka University
• Principal Investigator: 小林 剛 大阪大学, 微生物病研究所, 教授 (90324847)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Project Status: Completed (Fiscal Year 2023)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2023: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2022: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2021: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
• Keywords: ロタウイルス / ワクチン

Outline of Research at the Start

ロタウイルス（RV）は乳幼児に深刻な嘔吐・下痢症を引き起こす原因ウイルスであり、発展途上国を中心に年間約20万人の死亡例が報告されている。
本研究では、独自に開発したRV遺伝子改変技術を駆使することで、VP4およびNSP4遺伝子に変異を加えた組換えウイルスを作製し、RV感染における侵入機構ならびに下痢症発症機構の解明を行う。得られた知見を基に、RV感染制御プラットフォームの構築を目指す。

Outline of Annual Research Achievements

HT29細胞（ヒト結腸癌由来）とサルロタウイルスSA11株を使用したCRISPR-Cas9ゲノムワイドスクリーニングを実施した。この結果、ロタウイルス感染に関わる宿主因子の一つとしてTumor Associated Calcium Signal Transducer 2（TACSTD2）を同定した。TACSTD2は皮膚や肺などの上皮に発現する膜1回貫通型糖タンパク質である。TACSTD2遺伝子をノックアウトすると、ロタウイルスの感染性が抑制されることが観察された。さらに、ロタウイルス感染によってTACSTD2の開裂が認められ、この開裂がロタウイルス感染に重要な役割を果たしていることが示唆された。膜貫通型の糖タンパク質であるTACSTD2は、Matriptaseによって細胞膜上で開裂されることが知られている。ロタウイルス感染細胞におけるTACSTD2の経時的な開裂が観察されたが、MatriptaseのノックダウンまたはMatriptase阻害剤Nafamostatの存在下では、ロタウイルス感染時の開裂が抑制され、ロタウイルスの感染性が低下することが確認された。これらの結果より、細胞膜上のTACSTD2がMatriptaseによって開裂されることでロタウイルスの細胞侵入に寄与していることが示された。
サルロタウイルス（SA11株）をマウスで10回継代し、得られたマウス馴化ロタウイルスの性状を解析した。マウス馴化ロタウイルス（SA11m株）は、Balb/cマウスで親株と比較してウイルスの増殖性および下痢発症能が増強していた。3週齢のマウスでは、親株と比較して腸内で効率よく増殖することが観察された。SA11m株のシークエンス解析とリバースジェネティクスを用いた研究により、SA11m株の病原性増強に関与する分節遺伝子を同定した。

Research Progress Status

令和5年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和5年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Cytocidal Effect of Irradiation on Gastric Cancer Cells Infected with a Recombinant Mammalian Orthoreovirus Expressing a Membrane-Targeted KillerRed (2024)
  • Author(s): Shirasaka Yoshinori、Yamada Kentaro、Etoh Tsuyoshi、Noguchi Kazuko、Hasegawa Takumi、Ogawa Katsuhiro、Kobayashi Takeshi、Nishizono Akira、Inomata Masafumi
  • Journal Title: Pharmaceuticals Volume: 17 Issue: 1 Pages: 79-79
  • DOI: 10.3390/ph17010079
  • Peer Reviewed / Open Access
• [Journal Article] Vero cell-adapted SARS-CoV-2 strain shows increased viral growth through furin-mediated efficient spike cleavage (2024)
  • Author(s): Minami Shohei、Kotaki Tomohiro、Sakai Yusuke、Okamura Shinya、Torii Shiho、Ono Chikako、Motooka Daisuke、Hamajima Rina、Nouda Ryotaro、Nurdin Jeffery A.、Yamasaki Moeko、Kanai Yuta、Ebina Hirotaka、Maeda Yusuke、Okamoto Toru、Tachibana Taro、Matsuura Yoshiharu、Kobayashi Takeshi
  • Journal Title: Microbiology Spectrum Volume: 12 Issue: 4
  • DOI: 10.1128/spectrum.02859-23
  • Peer Reviewed / Open Access
• [Journal Article] Establishment of immortalized Egyptian Rousettus bat cell lines (2024)
  • Author(s): Bai Lanlan、Tani Tetsuya、Kobayashi Takeshi、Nouda Ryotaro、Kanai Yuta、Sano Yusuke、Takami Kazutoshi、Tomita Hiroshi、Sugano Eriko、Ozaki Taku、Kiyono Tohru、Fukuda Tomokazu
  • Journal Title: FEBS Open Bio Volume: - Issue: 4 Pages: 598-612
  • DOI: 10.1002/2211-5463.13781
  • Peer Reviewed / Open Access
• [Journal Article] Genotype Diversity of Enteric Viruses in Wastewater Amid the COVID-19 Pandemic (2023)
  • Author(s): Hoque Sheikh Ariful、Kotaki Tomohiro、Pham Ngan Thi Kim、Onda Yuko、Okitsu Shoko、Sato Shintaro、Yuki Yoshikazu、Kobayashi Takeshi、Maneekarn Niwat、Kiyono Hiroshi、Hayakawa Satoshi、Ushijima Hiroshi
  • Journal Title: Food and Environmental Virology Volume: 15 Issue: 2 Pages: 176-191
  • DOI: 10.1007/s12560-023-09553-4
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Effective SARS-CoV-2 replication of monolayers of intestinal epithelial cells differentiated from human induced pluripotent stem cells (2023)
  • Author(s): Minami Shohei、Matsumoto Naomi、Omori Hiroko、Nakamura Yutaka、Tamiya Shigeyuki、Nouda Ryotaro、Nurdin Jeffery A.、Yamasaki Moeko、Kotaki Tomohiro、Kanai Yuta、Okamoto Toru、Tachibana Taro、Ushijima Hiroshi、Kobayashi Takeshi、Sato Shintaro
  • Journal Title: Scientific Reports Volume: 13 Issue: 1 Pages: 11610-11610
  • DOI: 10.1038/s41598-023-38548-1
  • Peer Reviewed / Open Access
• [Journal Article] Efficient Selective Adsorption of SARS-CoV-2 via the Recognition of Spike Proteins Using an Affinity Spongy Monolith (2023)
  • Author(s): Takuya Kubo, Eisuke Kanao, Koki Ishida, Shohei Minami, Tetsuya Tanigawa, Ryosuke Mizuta, Yoshihiro Sasaki, Koji Otsuka, and Takeshi Kobayashi
  • Journal Title: Analytical Chemistry Volume: 95 Issue: 35 Pages: 13185-13190
  • DOI: 10.1021/acs.analchem.3c02097
  • Peer Reviewed
• [Journal Article] Genetic engineering strategy for generating a stable dsRNA virus vector using a virus-like codon-modified transgene (2023)
  • Author(s): Kanai Yuta、Onishi Misa、Yoshida Yukino、Kotaki Tomohiro、Minami Shohei、Nouda Ryotaro、Yamasaki Moeko、Enoki Yasutaka、Kobayashi Takeshi
  • Journal Title: Journal of Virology Volume: 97 Issue: 10
  • DOI: 10.1128/jvi.00492-23
  • Peer Reviewed
• [Journal Article] Electrolyzed hypochlorous acid water exhibits potent disinfectant activity against various viruses through irreversible protein aggregation (2023)
  • Author(s): Dianty Rahmi、Hirano Junki、Anzai Itsuki、Kanai Yuta、Hayashi Tsuyoshi、Morimoto Masae、Kataoka-Nakamura Chikako、Kobayashi Sakura、Uemura Kentaro、Ono Chikako、Watanabe Tokiko、Kobayashi Takeshi、Murakami Kosuke、Kikuchi Kenji、Hotta Kunimoto、Yoshikawa Toshikazu、Taguwa Shuhei、Matsuura Yoshiharu
  • Journal Title: Frontiers in Microbiology Volume: 14
  • DOI: 10.3389/fmicb.2023.1284274
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The Emergence and Widespread Circulation of Enteric Viruses Throughout the COVID-19 Pandemic: A Wastewater-Based Evidence (2023)
  • Author(s): Hoque Sheikh Ariful、Saito Hiroyuki、Akino Wakako、Kotaki Tomohiro、Okitsu Shoko、Onda Yuko、Kobayashi Takeshi、Hossian Tania、Khamrin Pattara、Motomura Kazushi、Maneekarn Niwat、Hayakawa Satoshi、Ushijima Hiroshi
  • Journal Title: Food and Environmental Virology Volume: 15 Issue: 4 Pages: 342-354
  • DOI: 10.1007/s12560-023-09566-z
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Characterization of Sialic Acid-Independent Simian Rotavirus Mutants in Viral Infection and Pathogenesis (2023)
  • Author(s): Yamasaki Moeko、Kanai Yuta、Wakamura Yoshiki、Kotaki Tomohiro、Minami Shohei、Nouda Ryotaro、Nurdin Jeffery A.、Kobayashi Takeshi
  • Journal Title: Journal of Virology Volume: 97 Issue: 1
  • DOI: 10.1128/jvi.01397-22
  • Peer Reviewed
• [Journal Article] N-Glycosylation of Rotavirus NSP4 Protein Affects Viral Replication and Pathogenesis. (2023)
  • Author(s): Nurdin JA, Kotaki T, Kawagishi T, Sato S, Yamasaki M, Nouda R, Minami S, Kanai Y, Kobayashi T.
  • Journal Title: Journal of Virology Volume: 97 Issue: 1
  • DOI: 10.1128/jvi.01861-22
  • Peer Reviewed
• [Journal Article] A reverse genetics system for human rotavirus G2P[4] (2022)
  • Author(s): Hamajima Rina、Lusiany Tina、Minami Shohei、Nouda Ryotaro、Nurdin Jeffery A.、Yamasaki Moeko、Kobayashi Nobumichi、Kanai Yuta、Kobayashi Takeshi
  • Journal Title: Journal of General Virology Volume: 103 Issue: 12 Pages: 001816-001816
  • DOI: 10.1099/jgv.0.001816
  • Peer Reviewed
• [Journal Article] Development of an oncolytic mammalian orthoreovirus expressing the near-infrared fluorescent protein iRFP720 (2022)
  • Author(s): Ogawa Katsuhiro、Yamada Kentaro、Etoh Tsuyoshi、Kitagawa Masahiro、Shirasaka Yoshinori、Noguchi Kazuko、Kobayashi Takeshi、Nishizono Akira、Inomata Masafumi
  • Journal Title: Journal of Virological Methods Volume: 308 Pages: 114574-114574
  • DOI: 10.1016/j.jviromet.2022.114574
  • Peer Reviewed
• [Journal Article] The nonstructural p17 protein of a fusogenic bat-borne reovirus regulates viral replication in virus species- and host-specific manners (2022)
  • Author(s): Nouda Ryotaro、Kawagishi Takahiro、Kanai Yuta、Shimojima Masayuki、Saijo Masayuki、Matsuura Yoshiharu、Kobayashi Takeshi
  • Journal Title: PLOS Pathogens Volume: 18 Issue: 6 Pages: e1010553-e1010553
  • DOI: 10.1371/journal.ppat.1010553
  • Peer Reviewed
• [Journal Article] Whole genome sequencing and evolutionary analysis of G8P [8] rotaviruses emerging in Japan (2022)
  • Author(s): Phan Tung、Kobayashi Masaaki、Nagasawa Koo、Hatazawa Riona、Thi Kim Pham Ngan、Miyashita Hideaki、Komoto Satoshi、Tajima Takeshi、Baba Tuneyoshi、Okitsu Shoko、Khamrin Pattara、Maneekarn Niwat、Kimura Hirokazu、Kobayashi Takeshi、Hayakawa Satoshi、Ushijima Hiroshi
  • Journal Title: VirusDisease Volume: 33 Issue: 2 Pages: 215-218
  • DOI: 10.1007/s13337-022-00765-z
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Whole genome sequence of an uncommon G9P[4] species A rotavirus containing DS-1-like (genotype 2) genes in Japan (2022)
  • Author(s): Phan Tung、Hatazawa Riona、Komoto Satoshi、Nishimura Shuichi、Khamrin Pattara、Pham Ngan Thi Kim、Okitsu Shoko、Kobayashi Takashi、Maneekarn Niwat、Hayakawa Satoshi、Ushijima Hiroshi
  • Journal Title: Archives of Virology Volume: 167 Issue: 7 Pages: 1603-1606
  • DOI: 10.1007/s00705-022-05462-2
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Emerging norovirus GII.4 Sydney[P31] causing acute gastroenteritis outbreak in children in Japan, during COVID-19, 2021 (2022)
  • Author(s): Pham Ngan Thi Kim、Nishimura Shuichi、Shimizu-Onda Yuko、Trinh Quang Duy、Komine-Aizawa Shihoko、Khamrin Pattara、Okitsu Shoko、Sato Shintaro、Kobayashi Takeshi、Maneekarn Niwat、Hayakawa Satoshi、Ushijima Hiroshi
  • Journal Title: Journal of Infection and Chemotherapy Volume: 28 Issue: 9 Pages: 1347-1351
  • DOI: 10.1016/j.jiac.2022.05.015
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An increasing trend of human sapovirus infection in Japan, 2009 to 2019: An emerging public health concern (2022)
  • Author(s): Hoque S. A., Nishimura K, Thongprachum A. , Khamrin P. ,Ngan Thi Kim Pham, Islam M. T. , Khandoker n. , Okitsu S, Onda-Shimizu Y , Dey S. K. ,Maneekarn N. , Kobayashi T, Hayakawa S, Ushijima H.
  • Journal Title: Journal of Infection and Public Health Volume: 15 Issue: 3 Pages: 315-320
  • DOI: 10.1016/j.jiph.2022.01.019
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] SARS-CoV-2 infection triggers paracrine senescence and leads to a sustained senescence-associated inflammatory response (2022)
  • Author(s): Tsuji Shunya、Minami Shohei、Hashimoto Rina、Konishi Yusuke、Suzuki Tatsuya、Kondo Tamae、Sasai Miwa、Torii Shiho、Ono Chikako、Shichinohe Shintaro、Sato Shintaro、et al.
  • Journal Title: Nature Aging Volume: 2 Issue: 2 Pages: 115-124
  • DOI: 10.1038/s43587-022-00170-7
  • Peer Reviewed / Open Access
• [Journal Article] Ultrasensitive detection of SARS-CoV-2 nucleocapsid protein using large gold nanoparticle-enhanced surface plasmon resonance (2022)
  • Author(s): Yano T, Kajisa T, Ono M, Miyasaka Y, Hasegawa Y, Saito A, Otsuka K, Sakane A, Sasaki T, Yasutomo K, Hamajima R, Kanai Y, Kobayashi T, Matsuura Y, Itonaga M, Yasui T.
  • Journal Title: Scientific Reports Volume: 12 Issue: 1 Pages: 1060-1060
  • DOI: 10.1038/s41598-022-05036-x
  • Peer Reviewed / Open Access
• [Journal Article] Species A rotavirus reverse genetics: Achievements and prospects (2021)
  • Author(s): Kobayashi T, Patton J.T., Desselberger U.
  • Journal Title: Virus Research Volume: 306 Pages: 198583-198583
  • DOI: 10.1016/j.virusres.2021.198583
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Development of an entirely plasmid-based reverse genetics system for 12-segmented double-stranded RNA viruses (2021)
  • Author(s): Nouda R, Minami S, Kanai Y, Kawagishi T, Nurdin JA, Yamasaki M, Kuwata R, Shimoda H, Maeda K, Kobayash T.
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 118 Issue: 42
  • DOI: 10.1073/pnas.2105334118
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Monoreassortant Rotaviruses of Multiple G Types Are Differentially Neutralized by Sera From Infants Vaccinated With ROTARIX and RotaTeq (2021)
  • Author(s): J. R. Diller, M. H. Carter, Kanai Y, S. V. Sanchez, Kobayashi T, K. M. Ogdena.
  • Journal Title: The Journal of Infectious Diseases Volume: - Issue: 10 Pages: 1720-1729
  • DOI: 10.1093/infdis/jiab479
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The Detection of Rotavirus Antigenemia by Immunochromatographic Kits: a Case Series. (2021)
  • Author(s): Ushijima H, Hikita T, Kobayashi M, Pham NTK, Onda-Shimizu Y, Kawagishi T, Okitsu S, Kanai Y, Kobayashi T, Phan T, Hoque SA, Takanashi S, Komoto S, Kumthip K, Taniguchi K, Maneekarn N, Hayakawa S, Khamrin P.
  • Journal Title: Clinical Laboratory Volume: 67 Issue: 10/2021 Pages: 2275-2282
  • DOI: 10.7754/clin.lab.2021.210125
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] FAST Proteins: Development and Use of Reverse Genetics Systems for Reoviridae Viruses (2021)
  • Author(s): Kanai Y, Kobayashi T.
  • Journal Title: Annual Review of Virology Volume: 8 Issue: 1 Pages: 515-536
  • DOI: 10.1146/annurev-virology-091919-070225
  • Peer Reviewed
• [Journal Article] Epidemiology and genetic diversity of group A rotavirus in pediatric patients with acute gastroenteritis in Thailand, 2018-2019 (2021)
  • Author(s): Nutthawadee J, Kattareeya K, Arpaporn Y, Kanai Y, Okitsu S, Kobayashi T, Nuthapong U, Ushijima H, Niwat M, Pattara K.
  • Journal Title: Infection, Genetics and Evolution Volume: 95 Pages: 104898-104898
  • DOI: 10.1016/j.meegid.2021.104898
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] DsRNA Sequencing for RNA Virus Surveillance Using Human Clinical Samples (2021)
  • Author(s): Izum T, Morioka Y, Urayama S, Motooka D, Tamura T, Kawagishi T, Kanai Y, Kobayashi T, Ono C, Morinaga A, Tomiyama T, Iseda N, Kosai Y, Inokuchi S, Nakamura S, Tanaka T, Moriishi K, Kariwa H, Yoshizumi T, Mori M, Matsuura Y, Fukuhara T.
  • Journal Title: Viruses Volume: 13 Issue: 7 Pages: 1310-1310
  • DOI: 10.3390/v13071310
  • Peer Reviewed / Open Access
• [Journal Article] Rotavirus reverse genetics systems: Development and application (2021)
  • Author(s): Kanai Y, Kobayashi T.
  • Journal Title: Virus Research Volume: 295 Pages: 198296-198296
  • DOI: 10.1016/j.virusres.2021.198296
  • Peer Reviewed
• [Journal Article] Combining machine learning and nanopore construction creates an artificial intelligence nanopore for coronavirus detection (2021)
  • Author(s): Taniguchi M, Minami S, Ono C, Hamajima R, Morimura A, Hamaguchi S, Akeda Y, Kanai Y, Kobayashi T, Kamitani W, Terada Y, Suzuki K, Hatori N, Yamagishi Y, Washizu N, Takei H, Sakamoto O, Naono N, Tatematsu K, Washio T, Matsuura Y, Tomono K.
  • Journal Title: Nature Communications Volume: 12 Issue: 1 Pages: 3726-3726
  • DOI: 10.1038/s41467-021-24001-2
  • Peer Reviewed / Open Access
• [Presentation] Development of a Single-Round Infectious Rotavirus as a Next-Generation Vaccine (2024)
  • Author(s): Kotaki T, Kanai Y, Onishi M, Minami S, Chen Z. , Nouda N, J. A. Nurdin, Yamasaki Y, Kobayashi T.
  • Organizer: The First Joint Symposium of AMED SCARDA Japan Initiative for World-leading Vaccine Research and Development Centers
  • Int'l Joint Research
• [Presentation] Rotavirus replication and pathogenesis (2024)
  • Author(s): Takeshi Kobayashi
  • Organizer: 2024 International Conference on Emerging Infectious Diseases (EID) in the Pacific Rim
  • Int'l Joint Research
• [Presentation] A virus-like codon modification strategy improves transgene genetic stability in double-stranded RNA virus vectors (2023)
  • Author(s): Kanai Y, Onishi M, Yoshida Y, Minami S, Kotaki T, Nouda R, Kobayashi T.
  • Organizer: 42nd Annual Meeting American Society for Virology
  • Int'l Joint Research
• [Presentation] Virological characterization of high-growth Vero cell-adapted SARS-CoV-2 strains (2023)
  • Author(s): Minami S, Kotaki T, Sakai Y, Okamura S, Torii S, Ono C, Motooka D, Nouda R, Yamasaki M, Kanai Y, Ebina H, Maeda Y, Okamoto T, Matsuura Y, Kobayashi T.
  • Organizer: 42nd Annual Meeting American Society for Virology
  • Int'l Joint Research
• [Presentation] Generation of the recombinant Colorado tick fever virus using a reverse genetics system (2023)
  • Author(s): Minami S, Nouda R, Sonoda S, Onishi M, Kotaki T, Kanai Y, Kobayashi T.
  • Organizer: 第21回あわじ感染と免疫国際フォーラム
• [Presentation] 分節型二本鎖RNAウイルスベクターの開発研究における最近の進展 (2023)
  • Author(s): 小林 剛
  • Organizer: 第29回日本遺伝子細胞治療学会
• [Presentation] VP6欠損による一回感染性ロタウイルスの作製 (2023)
  • Author(s): 小瀧将裕、金井祐太 、南昌平 、大西恵 、納田遼太郎 、山﨑萌子 、小林剛
  • Organizer: 第70回日本ウイルス学会学術集会
• [Presentation] コロラドダニ熱ウイルスにおけるリバースジェネティクス系の確立 (2023)
  • Author(s): 南 昌平、納田遼太郎、園田静夏、大西 恵、山﨑萌子、小瀧将裕、金井祐太、小林 剛
  • Organizer: 第70回日本ウイルス学会学術集会
• [Presentation] Whole Genome Characterization of HAstV5-HAstV8-HAstV1 Recombinant Strains of ORF1a-ORF1b-ORF2 Regions (2022)
  • Author(s): Pattara Khamrin, Kattareeya Kumthip, Shintaro Sato, Shoko Okitsu, Takeshi Kobayashi, Satoshi Hayakawa, Hiroshi Ushijima, Niwat Maneekarn
  • Organizer: 第68回日本ウイルス学会学術集会
• [Presentation] Recent advances in rotavirus reverse genetics research (2022)
  • Author(s): 小林剛
  • Organizer: 第68回日本ウイルス学会学術集会
  • Invited
• [Presentation] ロタウイルス感染に関与する宿主因子Tumor Associated Calcium Signal Transducer 2の機能解析 (2022)
  • Author(s): 山崎萌子、金井祐太、浜島りな、小瀧将裕、南昌平、納田遼太郎、Jeffery A. Nurdin、小林剛
  • Organizer: 第68回日本ウイルス学会学術集会
• [Presentation] ロタウイルスNSP2タンパク質の機能解析 (2022)
  • Author(s): 小瀧 将裕、金井 祐太、南 昌平、納田 遼太郎、Jeffery A. Nurdin、山﨑 萌子、小林 剛
  • Organizer: 第68回日本ウイルス学会学術集会
• [Presentation] マウス順化サルロタウイルスの作出 (2022)
  • Author(s): 金井 祐太、小瀧 将裕、南 昌平、納田 遼太郎、山﨑 萌子、小林 剛
  • Organizer: 第68回日本ウイルス学会学術集会
• [Presentation] ヒトロタウイルスHN126株におけるリバースジェネティクス系の確立 (2022)
  • Author(s): 浜島 りな、Tina Lusiany、南 昌平、納田 遼太郎、Jeffery A. Nurdin、山﨑 萌子、小林 宣道、金井 祐太、小林 剛
  • Organizer: 第68回日本ウイルス学会学術集会
• [Presentation] Single amino acid substitution in VP7 promotes the pathogenicity of simian rotavirus in mice (2022)
  • Author(s): Yuta Kanai, Eriko Hirai, Tomohiro Kotaki and Takeshi Kobayashi
  • Organizer: 14th International dsRNA Virus Symposium
  • Int'l Joint Research
• [Presentation] N-glycosylation of rotavirus NSP4 protein affects viral replication and pathogenesis (2022)
  • Author(s): Tomohiro Kotaki, Jeffery A. Nurdin, Takahiro Kawagishi, Shintaro Sato, Moeko Yamasaki, Ryotaro Nouda, Shohei Minami, Yuta Kanai, Takeshi Kobayashi
  • Organizer: 14th International dsRNA Virus Symposium
  • Int'l Joint Research
• [Presentation] Analysis of the role of rotavirus NSP4 protein in viral replication and pathogenicity (2022)
  • Author(s): Tomohiro Kotaki, Nurdin Jeffery A., Kawagishi Takahiro, Sato Shintaro, Yamasaki Moeko, Nouda Ryotaro, Minami Shohei, Kanai Yuta, Kobayashi Takeshi
  • Organizer: 第20回 あわじ感染と免疫国際フォーラム
  • Int'l Joint Research
• [Presentation] Identification of TACSTD2 as a host factor involved in rotavirus infection (2022)
  • Author(s): Moeko Yamasaki, Yuta Kanai, Rina Hamajima, Tomohiro Kotaki, Shohei Minami, Ryotaro Nouda, Takeshi Kobayashi.
  • Organizer: 第20回 あわじ感染と免疫国際フォーラム
  • Int'l Joint Research
• [Presentation] A genome-wide CRISPR-Cas9 screening approach to identify host factors for rotavirus infection CRISPR-Cas9法によるロタウイル ス感染に関与する宿主因子のゲノムワイドスクリーニング (2021)
  • Author(s): 山﨑 萌子、浜島 りな、金井 祐太、小瀧 将裕、南 昌平、納田 遼太郎、Nurdin Jeffery、小林 剛
  • Organizer: 第68回ウイルス学会学術集会
• [Presentation] Altered infectivity of rotaviruses in mice treated with antibiotics 抗生物質を投与したマウスにおけるロタウイルスの増殖能 (2021)
  • Author(s): 金井 祐太、Jeffery Nurdin、南 昌平、納田 遼太郎、山崎 萌子、小林 剛
  • Organizer: 第68回ウイルス学会学術集会
• [Presentation] Development of an entirely plasmid-based reverse genetics system for 12-segmented double-stranded RNA virus. (2021)
  • Author(s): Ryotaro Nouda, Shohei Minami, Yuta Kanai, Takahiro Kawagishi, Jeffery A. Nurdin, Moeko Yamasaki, Ryusei Kuwata, Hiroshi Shimoda, Ken Maeda, Takeshi Kobayashi.
  • Organizer: 第68回ウイルス学会学術集会
• [Presentation] Determining the role of rotavirus NSP4 N-glycosylation sites using reverse genetics approach (2021)
  • Author(s): Jeffery A. Nurdin, Yuta Kanai, Tomohiro Kotaki, Takahiro Kawagishi, Shohei Minami, Ryotaro Nouda, Moeko Yamasaki, Takeshi Kobayashi.
  • Organizer: 第68回ウイルス学会学術集会
• [Presentation] Analysis of intestinal epithelial cells differentiated from human induced pluripotent stem cells as a more suitable tool for the study of SARS-CoV-2 (2021)
  • Author(s): Shohei Minami, Naomi Matsumoto, Ryotaro Nouda, Jeffery Nurdin, Moeko Yamasaki, Tomohiro Kotaki, Yuta Kanai, Shintaro Sato, Takeshi Kobayashi.
  • Organizer: 第68回ウイルス学会学術集会
• [Book] "医学のあゆみ 285巻95 mRNAワクチンやゲノム編集で注目が集まる遺伝子治療 " (2023)
  • Author(s): 小瀧将裕、金井祐太、小林 剛
  • Total Pages: 6
  • Publisher: 医歯薬出版株式会社
• [Book] 医学のあゆみ280巻9号 ウイルスを創る，ウイルスを視る (2021)
  • Author(s): 金井祐太、納田遼太郎、小林 剛
  • Publisher: 医歯薬出版株式会社
• [Book] 創薬研究者がこれだけは知っておきたい最新のウイルス学 (2021)
  • Author(s): 金井祐太、納田遼太郎、山﨑萌子、小林 剛
  • Publisher: 技術情報協会
• [Remarks] 大阪大学微生物病研究所ウイルス免疫分野 小林研究室
  • URL: https://virology.biken.osaka-u.ac.jp/