Epigenetic regulation of lymphocyte cell division and oncogenesis

• Project/Area Number: 21H02751
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 49070:Immunology-related
• Research Institution: Hiroshima University
• Principal Investigator: Yasuda Tomoharu 広島大学, 医系科学研究科(医), 教授 (40334429)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Project Status: Completed (Fiscal Year 2023)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2023: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2022: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2021: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
• Keywords: B細胞 / T細胞 / PRC2 / 胚中心 / リンパ腫 / NKT細胞 / エピゲノム制御 / 免疫応答 / 細胞増殖 / B細胞分化 / T細胞分化 / 細胞分裂 / 細胞不死化 / 細胞老化 / 体細胞変異 / H3K27me3

Outline of Research at the Start

免疫細胞の分裂増殖は抗原特異的なクローンを増幅するだけでなく分化方向の決定とも連動することが知られており、免疫応答の特性を決定する重要要素である。免疫応答では抗原に反応したリンパ球が適切に細胞分裂を開始し適切なタイミングで停止されなければならないが、その制御メカニズムについては十分に解明されていない。本研究課題では癌遺伝子やウイルス抗原によって活性化したB細胞の不死化前後で発現が顕著に制御される因子として新規に見出した細胞老化や癌関連遺伝子群を足掛かりとしてリンパ腫発症の新たなメカニズムに迫るとともに、リンパ球分化を細胞分裂と連動して制御する分子基盤と免疫応答特性における役割の解明を目指す。

Outline of Final Research Achievements

The cell proliferation of immune cells is linked to the determination of their developmental direction and the characteristics of immune responses. We discovered the Eed gene, which constitutes the polycomb repressive complex PRC2, as an important regulator. We generated mice in which the Eed gene is deleted in T cells or B cells. We found that the thymic differentiation of iNKT cells is completely blocked and identified its specific molecular mechanisms. In germinal center B cell-specific Eed-deficient mice, we discovered that germinal center B cells, which are important for antibody affinity maturation, arrest at the early stage of differentiation, and the mechanism behind this was revealed.

Academic Significance and Societal Importance of the Research Achievements

本申請課題では免疫系の正常と病理に直結するリンパ球分裂増殖と分化誘導の制御機構に関し、相互に関連する細胞増殖、細胞死、不死化の分岐点となる分子実態の解明を目指し研究を行った。本研究成果をさらに発展させることで、将来的にがん治療、自己免疫疾患治療、新興感染症に対するワクチン開発など様々な医療分野で役立つ可能性がある。

Research Products

• [Journal Article] Precise CRISPR-Cas9 gene repair in autologous memory T cells to treat familial hemophagocytic lymphohistiocytosis (2024)
  • Author(s): Li Xun、Wirtz Tristan、Weber Timm、Lebedin Mikhail、Lowenstein Elijah D.、Sommermann Thomas、Zach Andreas、Yasuda Tomoharu、de la Rosa Kathrin、Chu Van Trung、Schulte Johannes H.、Muller Ingo、Kocks Christine、Rajewsky Klaus
  • Journal Title: Science Immunology Volume: 9 Issue: 92
  • DOI: 10.1126/sciimmunol.adi0042
  • Peer Reviewed / Open Access
• [Journal Article] Mouse models of human multiple myeloma subgroups (2023)
  • Author(s): Winkler Wiebke、Farre Diaz Carlota、Blanc Eric、Napieczynska Hanna、Langner Patrick、Werner Marvin、Walter Barbara、Wollert-Wulf Brigitte、Yasuda Tomoharu、Heuser Arnd、Beule Dieter、Mathas Stephan、Anagnostopoulos Ioannis、Rosenwald Andreas、Rajewsky Klaus、Janz Martin
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 120 Issue: 10
  • DOI: 10.1073/pnas.2219439120
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A senolytic immunotoxin eliminates p16-positive T cells and ameliorates age-associated phenotypes of CD4+ T cells in a surface marker knock-in mouse (2023)
  • Author(s): Sugiyama Yuma、Harada Tanenobu、Kamei Yuka、Yasuda Tomoharu、Mashimo Tomoji、Nishikimi Akihiko、Maruyama Mitsuo
  • Journal Title: Experimental Gerontology Volume: 174 Pages: 112130-112130
  • DOI: 10.1016/j.exger.2023.112130
  • Peer Reviewed / Open Access
• [Journal Article] Vaccination with the Omicron spike RBD boosts broadly neutralizing antibody levels and confers sustained protection even after acquiring immunity to the original antigen (2023)
  • Author(s): Hitoshi Azuma, Yohei Kawano, Kiyomi Shitaoka, Takahiro Kawahara, Ayano Ito, Akifumi Higashiura, Yasuo Kitajima, Shun Ohki, Tomoharu Yasuda
  • Journal Title: International Immunology Volume: 35 Issue: 4 Pages: 197-207
  • DOI: 10.1093/intimm/dxac055
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structural basis of spike RBM-specific human antibodies counteracting broad SARS-CoV-2 variants (2023)
  • Author(s): Kiyomi Shitaoka, Akifumi Higashiura, Yohei Kawano, Akima Yamamoto, Yoko Mizoguchi, Takao Hashiguchi , Norihisa Nishimichi, Shiyu Huang, Ayano Ito, Shun Ohki, Miyuki Kanda, Tomohiro Taniguchi, Rin Yoshizato, Hitoshi Azuma, Yasuo Kitajima, Yasuyuki Yokosaki, Satoshi Okada, Takemasa Sakaguchi, Tomoharu Yasuda
  • Journal Title: Communications Biology Volume: 6 Issue: 1 Pages: 1-14
  • DOI: 10.1038/s42003-023-04782-6
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] How Should We Deal with Neoplastic Disease and Serious Infections Caused by Epstein-Barr Virus ? (2023)
  • Author(s): Yoshiyama Hironori、Nanbo Asuka、Yasuda Tomoharu
  • Journal Title: Cancers Volume: 15 Issue: 11 Pages: 2889-2889
  • DOI: 10.3390/cancers15112889
  • Peer Reviewed / Open Access
• [Journal Article] Regulation mechanisms of <scp>CARMA1</scp> ?Bcl10? <scp>MALT1</scp> complex assembly inferred from the analysis of <scp>TRAF6</scp> ‐deficient cells (2023)
  • Author(s): Inoue Kentaro、Yasuda Tomoharu、Baba Yoshihiro、Yamamoto Tadashi、Kurosaki Tomohiro、Shinohara Hisaaki
  • Journal Title: Genes to Cells Volume: - Issue: 6 Pages: 411-421
  • DOI: 10.1111/gtc.13022
  • Peer Reviewed / Open Access
• [Journal Article] Concomitant Cytotoxic Effector Differentiation of CD4+ and CD8+ T Cells in Response to EBV-Infected B Cells (2022)
  • Author(s): Tamura Yumi、Yamane Keita、Kawano Yohei、Bullinger Lars、Wirtz Tristan、Weber Timm、Sander Sandrine、Ohki Shun、Kitajima Yasuo、Okada Satoshi、Rajewsky Klaus、Yasuda Tomoharu
  • Journal Title: Cancers Volume: 14 Issue: 17 Pages: 4118-4118
  • DOI: 10.3390/cancers14174118
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Efficient Isolation of Lymphocytes and Myogenic Cells from the Tissue of Muscle Regeneration (2022)
  • Author(s): Kitajima Yasuo、Tsukahara Ryoka、Nakamoto Shohei、Yasuda Tomoharu
  • Journal Title: Cells Volume: 11 Issue: 11 Pages: 1754-1754
  • DOI: 10.3390/cells11111754
  • Peer Reviewed / Open Access
• [Journal Article] Kastor and Polluks polypeptides encoded by a single gene locus cooperatively regulate VDAC and spermatogenesis. (2022)
  • Author(s): Mise S#, Matsumoto A#*, Shimada K, Hosaka T, Takahashi M, Ichihara K, Shimizu H, Shiraishi C, Saito D, Suyama M, Yasuda T, Ide T, Izumi Y, Bamba T, Kimura-Someya T, Shirouzu M, Miyata H, Ikawa M, Nakayama KI*.
  • Journal Title: Nat. Commun. Volume: 13(1) Issue: 1 Pages: 1071-1071
  • DOI: 10.1038/s41467-022-28677-y
  • Peer Reviewed / Open Access
• [Journal Article] Potential role of inducible GPR3 expression under stimulated T cell conditions (2022)
  • Author(s): Shiraki, H., Tanaka, S., Guo, Y., Harada, K., Hide, I, Yasuda, T. and Sakai, N
  • Journal Title: J. Pharmacol. Sci. Volume: 148 Issue: 3 Pages: 307-314
  • DOI: 10.1016/j.jphs.2022.01.005
  • Peer Reviewed / Open Access
• [Journal Article] A ubiquitin-like protein encoded by the “noncoding” RNA TINCR promotes keratinocyte proliferation and wound healing (2021)
  • Author(s): Nita Akihiro、Matsumoto Akinobu、Tang Ronghao、Shiraishi Chisa、Ichihara Kazuya、Saito Daisuke、Suyama Mikita、Yasuda Tomoharu、Tsuji Gaku、Furue Masutaka、Katayama Bumpei、Ozawa Toshiyuki、Murata Teruasa、Dainichi Teruki、Kabashima Kenji、Hatano Atsushi、Matsumoto Masaki、Nakayama Keiichi I.
  • Journal Title: PLOS Genetics Volume: 17 Issue: 8 Pages: 1009686-1009686
  • DOI: 10.1371/journal.pgen.1009686
  • Peer Reviewed / Open Access
• [Journal Article] Generation and characterization of CD19-iCre mice as a tool for efficient and specific conditional gene targeting in B cells. (2021)
  • Author(s): Yasuda T, Saito Y, Ono C, Kawata K, Baba A, Baba Y.
  • Journal Title: Sci. Rep. Volume: 11 Issue: 1 Pages: 5524-5524
  • DOI: 10.1038/s41598-021-84786-6
  • Peer Reviewed / Open Access
• [Journal Article] A conserved PI(4,5)P2-binding domain is critical for immune regulatory function of DOCK8 (2021)
  • Author(s): Sakurai Tetsuya、Kukimoto-Niino Mutsuko、Kunimura Kazufumi、Yamane Nana、Sakata Daiji、Aihara Ryosuke、Yasuda Tomoharu、Yokoyama Shigeyuki、Shirouzu Mikako、Fukui Yoshinori、Uruno Takehito
  • Journal Title: Life Science Alliance Volume: 4 Issue: 4 Pages: e202000873-e202000873
  • DOI: 10.26508/lsa.202000873
  • Peer Reviewed / Open Access
• [Presentation] 疾患関連バリアント特定のためのEBウイルスゲノム大規模解析による分類方法の検討 (2024)
  • Author(s): 福島大誠, 大木駿, 吉里倫, 保田朋波流
  • Organizer: 第32回EBV感染症研究会
• [Presentation] Immune Surveillance of Spontaneous Tumors Triggered by Latent Epstein-Barr Virus Infection (2024)
  • Author(s): Yuqi Jin, Yun Guo, Megumi Sasatani, Kenji Kamiya, Tomoharu Yasuda.
  • Organizer: The 8th International Symposium of the Network-type Joint Usage/Research Center for Radiation Disaster Medical Science
  • Int'l Joint Research
• [Presentation] Large-scale comparative analysis of Epstein-Barr virus genome to unveil geography- and disease-associated variations (2024)
  • Author(s): Taisei Fukushima, Shun Ohki, Rin Yoshizato, Tomoharu Yasuda.
  • Organizer: The 8th International Symposium of the Network-type Joint Usage/Research Center for Radiation Disaster Medical Science
  • Int'l Joint Research
• [Presentation] The characterization of cultured common lymphoid progenitor-derived monocytic cells (cCLP-M) (2024)
  • Author(s): Yohei Kawano, Mizuki Moriyama, Shun Ohki, Yasuo Kitajima, Tomoharu Yasuda
  • Organizer: The 52nd Annual Meeting of The Japanese Society for Immunology
  • Int'l Joint Research
• [Presentation] Sequential exposure to different SARS-CoV-2 antigens induces broadly neutralizing antibodies in mice. (2024)
  • Author(s): Hitoshi Azuma, Yohei Kawano, Akifumi Higashiura, Yasuo Kitajima, Shun Ohki, Tomoharu Yasuda
  • Organizer: The 52nd Annual Meeting of The Japanese Society for Immunology
  • Int'l Joint Research
• [Presentation] Immune Surveillance of Radiation-Induced Tumors Elicited by Latent Epstein-Barr Virus Oncogenes (2024)
  • Author(s): Yuqi Jin, Yun Guo, Tomoharu Yasuda.
  • Organizer: The 52nd Annual Meeting of The Japanese Society for Immunology
  • Int'l Joint Research
• [Presentation] The role of Polycomb group gene Eed in invariant NKT cell development and liver protection (2024)
  • Author(s): Yun Guo, Yohei Kawano, Masamoto Kanno, Tomoharu Yasuda
  • Organizer: The 52nd Annual Meeting of The Japanese Society for Immunology
  • Int'l Joint Research
• [Presentation] Acquisition of tumor-specific T cell immunity by wide-spread viral molecule (2024)
  • Author(s): Keita Yamane, Yohei Kawano, Shun Ohki, Yuri Matsuoka, Rin Yoshizato, Yumi Tamura, Yasuo Kitajima, Ryoka Tsukahara, Tomoharu Yasuda
  • Organizer: The 52nd Annual Meeting of The Japanese Society for Immunology
  • Int'l Joint Research
• [Presentation] Define The Direct Activation of Mycophenolate mofetil in Non-Hepatic Cells. (2023)
  • Author(s): Yusei Ota, Yuki Fujii, Hirotaka Kanoh, Rina Okihara, Mika Sasaki, Aki Ogawa-Iio, Sei Kondo, Keita Yamane, Yumi Tamura, Hirotoshi Ochi, Miki Senda, Kari Kopra, Harri Harma, Toshiya Senda, Koh Takeuchi, Tomoharu Yasuda, Atsuo Sasaki
  • Organizer: The 46th Annual Meeting of the Molecular Biology Society of Japan
  • Int'l Joint Research
• [Presentation] 培養共通リンパ球系前駆細胞由来単球／マクロファージ(cCLP-M)の性状及び機能解析 (2023)
  • Author(s): 河野 洋平, 森山 瑞月, 大木 駿, 北嶋 康雄, 保田 朋波流
  • Organizer: 第46回日本分子生物学会
  • Int'l Joint Research
• [Presentation] 大規模なEBV遺伝子配列の比較解析による地域・疾患関連変異の特定 (2023)
  • Author(s): 福島大誠, 大木駿, 吉里倫, 保田朋波流
  • Organizer: 第46回日本分子生物学会
  • Int'l Joint Research
• [Presentation] 次世代シーケンスによるアナフィラキシー誘発性IgE抗体産生経路の可視化 (2023)
  • Author(s): 大木駿, 河原隆浩, 河野洋平, 保田朋波流
  • Organizer: 第46回日本分子生物学会
  • Int'l Joint Research
• [Presentation] 骨格筋再生におけるB細胞の役割解明 (2023)
  • Author(s): 北嶋康雄, 塚原涼火, 河野洋平, 保田朋波流
  • Organizer: 第46回日本分子生物学会
  • Int'l Joint Research
• [Presentation] 骨格筋はCaMK2を介して運動負荷に適応する (2023)
  • Author(s): 塚原涼火, 北嶋康雄, 河野洋平, 保田朋波流
  • Organizer: 第46回日本分子生物学会
  • Int'l Joint Research
• [Presentation] Epstein-Barrウイルス分子LMP1を用いた固形がん特異的細胞傷害性T細胞の誘導 (2023)
  • Author(s): 山根慶大, 河野洋平, 大木駿, 松岡祐里, 吉里倫, 田村結実, 北嶋康雄, 塚原涼火, 福島大誠, 保田朋波流
  • Organizer: 第46回日本分子生物学会
  • Int'l Joint Research
• [Presentation] 回復者由来SARS-CoV-2中和抗体-スパイクタンパク質複合体の単粒子解析 (2023)
  • Author(s): 野間井 智, 安楽 佑樹, 山本 旭麻, 東浦 彰史, 下岡 清美, 河野 洋平, 喜多 俊介, 橋口 隆生, 保田 朋波流, 坂口 剛正, 前仲 勝実
  • Organizer: 生命分子科学研究会
• [Presentation] SARS-CoV-2スパイク蛋白質と広域中和抗体の複合体構造解析 (2023)
  • Author(s): 山本旭麻, 東浦彰史, 下岡清美, 河野洋平, 喜多俊介, 安楽佑樹, 野間井智, 橋口隆生, 前仲勝実, 保田朋波流, 坂口剛正
  • Organizer: 日本結晶学会
• [Presentation] SARS-CoV-2のNタンパク質を中心とした構造生物学研究 (2023)
  • Author(s): 東浦彰史, 山本旭麻, 伊藤遼樹, 吉里倫, 保田朋波流, 坂口剛正
  • Organizer: 日本結晶学会
• [Presentation] SARS CoV-2スパイク蛋白質と各種中和抗体の複合体構造解析より明らかとなった中和活性メカニズム (2023)
  • Author(s): 山本旭麻, 東浦彰史, 下岡清美, 河野洋平, 喜多俊介, 安楽佑樹, 橋口隆生, 前仲勝実, 保田朋波流, 坂口剛正
  • Organizer: 第70回日本ウイルス学会
• [Presentation] pstein-Barr ウイルス分子LMP1による固形がん免疫監視誘導と治療応用 (2023)
  • Author(s): 山根慶大, 河野洋平, 松岡祐里, 吉里倫, 田村結美, 北嶋康雄, 保田朋波流
  • Organizer: 第82回日本癌学会学術総会
  • Int'l Joint Research
• [Presentation] Epstein-Barrウイルス分子LMP1による固形がん免疫原性上昇作用の解明 (2023)
  • Author(s): 山根慶大, 河野洋平, 松岡祐里, 吉里倫, 田村結美, 北嶋康雄, 保田朋波流
  • Organizer: 第43回阿蘇シンポジウム
• [Presentation] 新型コロナウイルス広域中和抗体の構造生物学的研究 (2023)
  • Author(s): 24.東浦彰史, 山本旭麻, 下岡清美, 河野洋平, 保田朋波流, 坂口剛正
  • Organizer: 第36回 インフルエンザ研究者交流の会シンポジウム
• [Presentation] 抗腫瘍細胞傷害活性を有するCD4陽性T細胞の性状と制御機構 (2023)
  • Author(s): 田村結実，山根慶大, 森萌花，河野洋平, 大木駿, 北嶋康雄, 保田朋波流
  • Organizer: 第20回日本免疫治療学会
• [Presentation] 新型コロナウイルスに対する広域中和抗体 (2023)
  • Author(s): 保田朋波流
  • Organizer: 第20回日本免疫治療学会
  • Invited
• [Presentation] CAEBVに対する細胞表面抗原を標的とした治療法の開発 (2023)
  • Author(s): 吉里 倫, 山根 慶大, 保田朋波流
  • Organizer: 第31回EBV感染症研究会
• [Presentation] ゲノム編集技術を用いた免疫原性制御によるがん免疫療法の開発 (2022)
  • Author(s): 山根慶大, 河野洋平, 保田朋波流
  • Organizer: 第7回日本ゲノム編集学会
• [Presentation] Efficient CRISPR/Cas9-mediated precise gene repair in T cells to treat EBV-triggered primary HLH in mice and human (2022)
  • Author(s): Li, X., Wirtz, T., Weber, T., Lowenstein, E.D., Sommermann, T., Tran, N.T., Danner, E., Graf, R., Zach, A., Lebedin, M., Yasuda, T., Kocks, C., de la Rosa, K., Kuhn, R., Chu, V.T., and Rajewsky, K.
  • Organizer: International LIT Symposium
  • Int'l Joint Research
• [Presentation] 立体構造解析から明らかとなったSARS CoV-2中和抗体とスパイク蛋白質の相互作用様式 (2022)
  • Author(s): 東浦彰史、下岡清美、河野洋平、山本旭麻、保田朋波流、坂口剛正
  • Organizer: 第36回中四国ウイルス研究会
• [Presentation] SARS CoV-2スパイク蛋白質と中和抗体の複合体構造解析 (2022)
  • Author(s): 東浦彰史、下岡清美、河野洋平、山本旭麻、保田朋波流、坂口剛正
  • Organizer: 第69回日本ウイルス学会
• [Presentation] SARS-CoV-2構造タンパク質の構造生物学的研究 (2022)
  • Author(s): 山本旭麻、東浦彰史、伊藤遼樹、上岡史生子、下岡清美、河野洋平、保田朋波流、坂口剛正
  • Organizer: 日本結晶学会
• [Presentation] EBウイルス感染細胞により活性化されるヒト末梢血CD4 T細胞の解析 (2022)
  • Author(s): 田村結実，森萌花，北嶋康雄，河野洋平，保田朋波流
  • Organizer: 第45回日本分子生物学会
• [Presentation] マウスEBウイルス感染モデルでのCD4+T, CD8+T細胞の細胞傷害性分化の類似性 (2022)
  • Author(s): 山根慶大, 河野洋平，大木駿, 保田朋波流
  • Organizer: 第45回日本分子生物学会
• [Presentation] ゲノム縮約ライブラリーを対象にしたSNP検出におけるシーケンサー特異性の比較 (2022)
  • Author(s): 大木駿、北ノ坊誠也、松本悠貴、赤司亜弥子、守田昌哉、保田朋波流
  • Organizer: 第45回日本分子生物学会
• [Presentation] 骨格筋再生組織からのリンパ球および筋細胞の効率的な単離方法の確立 (2022)
  • Author(s): 北嶋康雄、塚原涼火、河野洋平、保田朋波流
  • Organizer: 第45回日本分子生物学会
• [Presentation] 培養共通リンパ系前駆細胞由来ミエロイド系細胞(cCLP-M)の性状及び機能解析 (2022)
  • Author(s): 河野洋平、森山瑞月、大木駿、北嶋康雄、保田朋波流
  • Organizer: 第45回日本分子生物学会
• [Presentation] Polycomb group gene Eed is required for invariant NKT development and maturation. (2022)
  • Author(s): 郭 芸, 菅野 雅元, 保田 朋波流
  • Organizer: 第51回日本免疫学会学術集会
• [Presentation] Concomitant cytotoxic effector differentiation of CD4+ and CD8+ T cells in response to EBV infected B cells. (2022)
  • Author(s): Yumi Tamura, Keita Yamane, Moeka Mori, Yohei Kawano, Shun Ohki, Yasuo Kitajima, Tomoharu Yasuda.
  • Organizer: The 51st Annual Meeting of the Japanese Society for Immunology
• [Presentation] Properties and functional analysis of cultured common lymphoid progenitor-derived monocytic cells (cCLP-M). (2022)
  • Author(s): Yohei Kawano, Mizuki Moriyama, Shun Ohki, Yasuo Kitajima, Tomoharu Yasuda.
  • Organizer: The 51th Annual Meeting of The Japanese Society for Immunology
• [Presentation] 感染症治療薬として期待される人工中和抗体 (2021)
  • Author(s): 保田朋波流
  • Organizer: 第72回コロイドおよび界面化学討論会「新型コロナ感染症対策とコロイド・界面化学」
  • Invited
• [Presentation] 免疫グロブリンの構造と生理的役割 (2021)
  • Author(s): 保田朋波流
  • Organizer: 第 29 回日本組織適合性学会大会
  • Invited
• [Presentation] 新型コロナウイルス感染症治療における中和抗体の活用 (2021)
  • Author(s): 保田朋波流、下岡清美、河野洋平
  • Organizer: 第12回日本RNAi研究会
  • Invited
• [Book] EBウイルス関連胃癌 改訂第2版, 「EBウイルス関連腫瘍における免疫治療」 (2022)
  • Author(s): 田村結実, 保田朋波流
  • Total Pages: 109
  • Publisher: 診断と治療社
  • ISBN: 9784787825698
• [Patent(Industrial Property Rights)] 抗SARS-CoV-2抗体及びその取得方法 (2021)
  • Inventor(s): 保田朋波流・下岡清美・他4名
  • Industrial Property Rights Holder: 広島大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2021-118204
  • Filing Date: 2021