• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Comprehensive search for mechanisms of resistance to molecularly targeted therapies

Research Project

Project/Area Number 21H02795
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 50020:Tumor diagnostics and therapeutics-related
Research InstitutionNational Cancer Center Japan

Principal Investigator

Kohsaka Shinji  国立研究開発法人国立がん研究センター, 研究所, 分野長 (00627119)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2023: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2022: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2021: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Keywordsゲノム医学 / 薬剤耐性 / がん遺伝子 / ハイスループット機能解析
Outline of Research at the Start

保険医療によるがん遺伝子パネル検査が普及しつつあるが, 機能不明のバリアントの生物学的・臨床的意味づけを行うことが, 個別化医療の実現に向けた大きな課題となっている. 我々はハイスループットがん遺伝子機能解析法 (mixed-all-nominated-mutants-in-one method: MANO法)を開発した. 本研究開発においてはがん遺伝子の網羅的機能解析, MANO法を用いた分子標的薬耐性化メカニズムの解明, 併用療法による耐性化解除の検討, リキッドバイオプシーを用いた薬剤耐性化メカニズムの解明をマイルストーンとし, 薬剤耐性を克服する新たな治療法の開発を目指す.

Outline of Final Research Achievements

As a comprehensive functional analysis of oncogenes, we evaluated the oncogenic potential of 68 genes and 243 variants. Furthermore, we evaluated the drug sensitivity of the 243 variants to molecularly targeted drugs and identified several resistance markers to molecularly targeted drugs. In addition, mRNA and protein phosphorylation were analyzed to evaluate the mechanism of resistance. To overcome resistance by combination therapy, we evaluated the optimal combination therapy using a carcinogenesis model in which a combination of MAP2K1 mutations caused carcinogenesis. To elucidate the mechanism of drug resistance using liquid biopsy, target sequencing analysis was performed using cfDNA present in plasma to identify biomarkers associated with resistance and response.

Academic Significance and Societal Importance of the Research Achievements

本研究開発において構築されたナレッジデータベースはゲノム医療に活用され、本国における臨床試験の促進につながると共に、新規治療標的およびバイオマーカーの同定が進むことが期待される。特に複数のドライバー遺伝子ががん化や腫瘍の進展に寄与することは単剤での治療反応性の低下に寄与していることが考えられるため、本研究で開発した網羅的機能解析プラットフォームを用いて、高精度な大規模データを取得していくことが、がんゲノム知識データベースの充実ならびに真の個別化医療の実践につながると考えられる。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • Research Products

    (88 results)

All 2024 2023 2022 2021

All Journal Article (49 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 49 results,  Open Access: 44 results) Presentation (38 results) (of which Int'l Joint Research: 4 results,  Invited: 9 results) Book (1 results)

  • [Journal Article] Analysis of quality metrics in comprehensive cancer genomic profiling using a dual DNA-RNA panel2024

    • Author(s)
      Watanabe K, Kohsaka S, Tatsuno K, Shinozaki-Ushiku A, Isago H, Kage H, Ushiku T, Aburatani H, Mano H, Oda K
    • Journal Title

      Pract Lab Med

      Volume: 39 Pages: 368-368

    • DOI

      10.1016/j.plabm.2024.e00368

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The development of a custom RNA-sequencing panel for the identification of predictive and diagnostic biomarkers in glioma2024

    • Author(s)
      Shirai Y, Ueno T, Kojima S, Ikeuchi H, Kitada R, Koyama T, Takahashi F, Takahashi K, Ichimura K, Yoshida A, Sugino H, Mano H, Narita Y, Takahashi M, Kohsaka S
    • Journal Title

      Journal of Neuro-Oncology

      Volume: 167 Issue: 1 Pages: 75-88

    • DOI

      10.1007/s11060-024-04563-z

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A Learning Program for Treatment Recommendations by Molecular Tumor Boards and Artificial Intelligence2024

    • Author(s)
      Sunami Kuniko、Naito Yoichi、Koyama Takafumi et.al
    • Journal Title

      JAMA Oncology

      Volume: 10 Issue: 1 Pages: 95-95

    • DOI

      10.1001/jamaoncol.2023.5120

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Inflammatory Rhabdomyoblastic Tumor: Clinicopathologic and Molecular Analysis of 13 Cases2024

    • Author(s)
      Odate T, Satomi K, Kubo T, Matsushita Y, Ueno T, Kurose A, Shomori K, Nakai T, Watanabe R, Segawa K, Ohshika S, Miyake N, Kudo S, Shimoi T, Kobayashi E, Komiyama M, Yoshimoto S, Nakatani F, Kawai, A, Yatabe Y, Kohsaka S, Ichimura K, Ichikawa H, Yoshida Akihiko
    • Journal Title

      Modern Pathology

      Volume: 37 Issue: 1 Pages: 100359-100359

    • DOI

      10.1016/j.modpat.2023.100359

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Multiomic molecular characterization of the response to combination immunotherapy in MSS/pMMR metastatic colorectal cancer2024

    • Author(s)
      Takei S、Tanaka Y、Lin Yi-Tzu、Koyama S、Fukuoka S、Hara H、Nakamura Y、Kuboki Y、Kotani D、Kojima T、Bando H、Mishima S、Ueno T、Kojima S、Wakabayashi M、Sakamoto N、Kojima M、Kuwata T、Yoshino T、Nishikawa H、Mano H、Endo I、Shitara K、Kawazoe A
    • Journal Title

      Journal for ImmunoTherapy of Cancer

      Volume: 12 Issue: 2 Pages: 8210-8210

    • DOI

      10.1136/jitc-2023-008210

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Case report and literature review: exploration of molecular therapeutic targets in recurrent malignant meningioma through comprehensive genetic analysis with Todai OncoPanel2023

    • Author(s)
      Ohara K, Miyawaki S, Nakatomi H, Okano A, Teranishi Y, Shinya Y, Ishigami D, Hongo H, Takayanagi S, Tanaka S, Shinozaki-Ushiku A, Kohsaka S, Kage H, Oda K, Miyagawa K, Aburatani H, Mano H, Tatsuno K, Saito N
    • Journal Title

      Front Neurol

      Volume: 14 Pages: 1270046-1270046

    • DOI

      10.3389/fneur.2023.1270046

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Promoter swapping of truncated PDGFRB drives Ph-like acute lymphoblastic leukemia2023

    • Author(s)
      Miyazaki Bunpei、Ueno Toshihide、Sugiyama Masanaka、Kojima Shinya、Arakawa Ayumu、Tao Kayoko、Tanimura Kazuki、Shiraishi Kouya、Yagishita Shigehiro、Kohsaka Shinji、Kato Mamoru、Kiyokawa Nobutaka、Goto Yasushi、Yatabe Yasushi、Hamada Akinobu、Mano Hiroyuki、Ogawa Chitose、Tanaka Yosuke
    • Journal Title

      npj Precision Oncology

      Volume: 7 Issue: 1 Pages: 132-132

    • DOI

      10.1038/s41698-023-00485-7

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Detection of Novel Tyrosine Kinase Fusion Genes as Potential Therapeutic Targets in Bone and Soft Tissue Sarcomas Using DNA/RNA-based Clinical Sequencing2023

    • Author(s)
      Hasegawa Nobuhiko、Hayashi Takuo、Niizuma Hidetaka、Kikuta Kazutaka、Imanishi Jungo、Endo Makoto、Ikeuchi Hiroshi、Sasa Keita、Sano Kei、Hirabayashi Kaoru、Takagi Tatsuya、Ishijima Muneaki、Kato Shunsuke、Kohsaka Shinji、Saito Tsuyoshi、Suehara Yoshiyuki
    • Journal Title

      Clinical Orthopaedics & Related Research

      Volume: 482 Issue: 3 Pages: 549

    • DOI

      10.1097/corr.0000000000002901

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Subclonal accumulation of immune escape mechanisms in microsatellite instability-high colorectal cancers2023

    • Author(s)
      Kobayashi Y, Niida A, Nagayama S, Saeki K, (中略), Mizuno S, Kawazu M, Kohsaka S, Ueno T, Mano H, Ishihara S, Uemura M, Mori M, Doki Y, Eguchi H, Oshima M, Suzuki Y, Shibata T, Mimori K.
    • Journal Title

      Br J Cancer

      Volume: 129 Issue: 7 Pages: 1105-1118

    • DOI

      10.1038/s41416-023-02395-8

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Extensive analysis of 59 sarcoma‐related fusion genes identified pazopanib as a potential inhibitor to <scp><i>COL1A1‐PDGFB</i></scp> fusion gene2023

    • Author(s)
      Hirose Takeshi、Ikegami Masachika、Kojima Shinya、Yoshida Akihiko、Endo Makoto、Shimada Eijiro、Kanahori Masaya、Oyama Ryunosuke、Matsumoto Yoshihiro、Nakashima Yasuharu、Kawai Akira、Mano Hiroyuki、Kohsaka Shinji
    • Journal Title

      Cancer Science

      Volume: 114 Issue: 10 Pages: 4089-4100

    • DOI

      10.1111/cas.15915

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Clinical Activity and Exploratory Resistance Mechanism of Milademetan, an MDM2 Inhibitor, in Intimal Sarcoma with <i>MDM2</i> Amplification: An Open-Label Phase Ib/II Study2023

    • Author(s)
      Koyama Takafumi、Shimizu Toshio、Kojima Yuki、Sudo Kazuki、Okuma Hitomi Sumiyoshi、Shimoi Tatsunori、Ichikawa Hitoshi、Kohsaka Shinji、Sadachi Ryo、Hirakawa Akihiro、Yoshida Akihiko、Ando Reiko Makihara、Ueno Toshihide、Yanagaki Mitsuru、Matsui Naoko、Nakamura Kenichi、Yamamoto Noboru、Yonemori Kan
    • Journal Title

      Cancer Discovery

      Volume: 13 Issue: 8 Pages: 1814-1825

    • DOI

      10.1158/2159-8290.cd-23-0419

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The pathogenic role of the BRCA2 c.7847C>T (p.Ser2616Phe) variant in breast and ovarian cancer predisposition2023

    • Author(s)
      Yamazawa Kazuki、Sugano Kokichi、Tanakaya Kohji、Inoue Satomi、Murakami Haruka、Nakashima Moeko、Adachi Masataka、Oki Shinya、Makabe Takeshi、Yamashita Hiroshi、Ueki Arisa、Sasaoka Ayako、Nakashoji Ayako、Kinoshita Takayuki、Matsunaga Tatsuo、Arai Masami、Nakamura Seigo、Miyata Hiroaki、Ikegami Masachika, et al.
    • Journal Title

      Cancer Science

      Volume: - Issue: 7 Pages: 2993-3002

    • DOI

      10.1111/cas.15799

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular pathology quality control in Southeast Asia: Results of a multiregional quality assurance study from MASTER KEY Asia2023

    • Author(s)
      Okuma Hitomi Sumiyoshi、Yoshida Hiroshi、Kobayashi Yoshihisa、Arakaki Motoko、Mizoguchi Chiharu、Inagaki Lina、Voon Pei Jye、Malik Bin Ismail Adam、Fen Soo Hoo Hwoei、Yusak Suhana、Severino B. Imasa Marcelo、Nguyen Huy Thinh、Thai Anh Tu、Kohsaka Shinji、Mano Hiroyuki、Yonemori Kan、Nakamura Kenichi、Yatabe Yasushi
    • Journal Title

      Cancer Science

      Volume: in press Issue: 6 Pages: 2664-2673

    • DOI

      10.1111/cas.15790

    • Related Report
      2023 Annual Research Report 2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Clinical utility of Todai OncoPanel in the setting of approved comprehensive cancer genomic profiling tests in Japan2023

    • Author(s)
      Kage Hidenori、Shinozaki‐Ushiku Aya、Tanikawa Michihiro、Aburatani Hiroyuki、Mano Hiroyuki、Miyagawa Kiyoshi、Oda Katsutoshi
    • Journal Title

      Cancer Science

      Volume: 114 Issue: 4 Pages: 1710

    • DOI

      10.1111/cas.15717

    • Related Report
      2023 Annual Research Report 2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Expression of paired box 9 defines an aggressive subset of lung adenocarcinoma preferentially occurring in smokers2023

    • Author(s)
      Hayashi Takuo、Kishi Monami、Takamochi Kazuya、Hosoya Masaki、Kohsaka Shinji、Kishikawa Satsuki、Ura Ayako、Sano Kei、Sasahara Noriko、Suehara Yoshiyuki、Takahashi Fumiyuki、Saito Tsuyoshi、Suzuki Kenji、Yao Takashi、the Tokyo Metropolitan Innovative Oncology Research Group (TMIG)
    • Journal Title

      Histopathology

      Volume: 82 Issue: 5 Pages: 672-683

    • DOI

      10.1111/his.14853

    • Related Report
      2023 Annual Research Report 2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genome Doubling Shapes High-Grade Transformation and Novel EWSR1::LARP4 Fusion Shows SOX10 Immunostaining in Hyalinizing Clear Cell Carcinoma of Salivary Gland2023

    • Author(s)
      Kobayashi Kenya、Kawazu Masahito、Yoshimoto Seiichi、Ueno Toshihide、Omura Go、Saito Yuki、Ando Mizuo、Ryo Eigitsu、Sakyo Airi、Yoshida Akihiko、Yatabe Yasushi、Mano Hiroyuki、Mori Taisuke
    • Journal Title

      Laboratory Investigation

      Volume: 103 Issue: 10 Pages: 100213-100213

    • DOI

      10.1016/j.labinv.2023.100213

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Strategic Approach to Heterogeneity Analysis of Cutaneous Adnexal Carcinomas Using Computational Pathology and Genomics2023

    • Author(s)
      Nishimura Yuuki、Ryo Eijitsu、Inoue Satoshi、Kawazu Masahito、Ueno Toshihide、Namikawa Kenjiro、Takahashi Akira、Ogata Dai、Yoshida Akihiko、Yamazaki Naoya、Mano Hiroyuki、Yatabe Yasushi、Mori Taisuke
    • Journal Title

      JID Innovations

      Volume: 3 Issue: 6 Pages: 100229-100229

    • DOI

      10.1016/j.xjidi.2023.100229

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dramatic response to entrectinib in a patient with malignant peripheral nerve sheath tumor harboring novel SNRNP70-NTRK3 fusion gene2023

    • Author(s)
      Kobayashi H, Makise N, Shinozaki-Ushiku A, Zhang L, Ishibashi Y, Ikegami M, Tsuda Y, Kohsaka S, Ushiku T, Oda K, Miyagawa K, Aburatani H, Mano H, Tanaka S.
    • Journal Title

      Genes Chromosomes Cancer.

      Volume: 62 Issue: 1 Pages: 47-51

    • DOI

      10.1002/gcc.23089

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Concordance Between Recommendations From Multidisciplinary Molecular Tumor Boards and Central Consensus for Cancer Treatment in Japan.2022

    • Author(s)
      Naito Y, Sunami K, Kage H, Komine K, Amano T, Imai M, Koyama T, Ennishi D, Kanai M, Kenmotsu H, Maeda T, Morita S, Sakai D, Watanabe K, Shirota H, Kinoshita I, Yoshioka M, Mamesaya N, Ito M, Kohsaka S, Saigusa Y, Yamamoto K, Hirata M, Tsuchihara K, Yoshino T.
    • Journal Title

      JAMA Netw Open.

      Volume: 5 Issue: 12 Pages: 2245081-2245081

    • DOI

      10.1001/jamanetworkopen.2022.45081

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] High-Throughput Functional Evaluation of MAP2K1 Variants in Cancer2022

    • Author(s)
      Mizuno Sho、Ikegami Masachika、Koyama Takafumi、Sunami Kuniko、Ogata Dai、Kage Hidenori、Yanagaki Mitsuru、Ikeuchi Hiroshi、Ueno Toshihide、Tanikawa Michihiro、Oda Katsutoshi、Osuga Yutaka、Mano Hiroyuki、Kohsaka Shinji
    • Journal Title

      Molecular Cancer Therapeutics

      Volume: 22 Issue: 2 Pages: 227-239

    • DOI

      10.1158/1535-7163.mct-22-0302

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of novel prognostic and predictive biomarkers in salivary duct carcinoma via comprehensive molecular profiling2022

    • Author(s)
      Kohsaka S, Tada Y, Okami K, et al.
    • Journal Title

      NPJ Precis Oncol.

      Volume: 6 Issue: 1 Pages: 82-82

    • DOI

      10.1038/s41698-022-00324-1

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] C-CAT: The National Datacenter for Cancer Genomic Medicine in Japan2022

    • Author(s)
      Kohno T, Kato M, Kohsaka S, Sudo T, Tamai I, Shiraishi Y, Okuma Y, Ogasawara D, Suzuki T, Yoshida T, Mano H
    • Journal Title

      Cancer Discov

      Volume: 12 Issue: 11 Pages: 2509-2515

    • DOI

      10.1158/2159-8290.cd-22-0417

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Primary Intracranial Spindle Cell Sarcoma, DICER1-Mutant with MDM2 Amplification Diagnosed on the Basis of Extensive Molecular Profiling2022

    • Author(s)
      Nejo Takahide、Takayanagi Shunsaku、Tanaka Shota、Shinozaki-Ushiku Aya、Kohsaka Shinji、Nagata Keisuke、Yokoyama Munehiro、Sora Shigeo、Ushiku Tetsuo、Mukasa Akitake、Aburatani Hiroyuki、Mano Hiroyuki、Saito Nobuhito
    • Journal Title

      Clinical Medicine Insights: Case Reports

      Volume: 15 Pages: 117954762211311-117954762211311

    • DOI

      10.1177/11795476221131189

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Novel Calcium-Binding Ablating Mutations Induce Constitutive RET Activity and Drive Tumorigenesis2022

    • Author(s)
      Tabata Junya、Nakaoku Takashi、Araki Mitsugu、Yoshino Ryunosuke、Kohsaka Shinji、Otsuka Ayaka、Ikegami Masachika、Ui Ayako、Kanno Shin-ichiro、Miyoshi Keiko、Matsumoto Shigeyuki、Sagae Yukari、Yasui Akira、Sekijima Masakazu、Mano Hiroyuki、Okuno Yasushi、Okamoto Aikou、Kohno Takashi
    • Journal Title

      Cancer Research

      Volume: 82 Issue: 20 Pages: 3751-3762

    • DOI

      10.1158/0008-5472.can-22-0834

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Survival benefit of HER2-targeted or androgen deprivation therapy in salivary duct carcinoma2022

    • Author(s)
      Kawakita Daisuke、Nagao Toshitaka、Takahashi Hideaki、Kano Satoshi、Honma Yoshitaka、Hirai Hideaki、Saigusa Natsuki、Akazawa Kohei、Tani Kaori、Ojiri Hiroya、Tsukahara Kiyoaki、Ozawa Hiroyuki、Okami Kenji、Kondo Takahito、Togashi Takafumi、Fushimi Chihiro、Shimura Tomotaka、Matsuki Takashi、Tada Yuichiro
    • Journal Title

      Therapeutic Advances in Medical Oncology

      Volume: 14 Pages: 175883592211195-175883592211195

    • DOI

      10.1177/17588359221119538

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Chronological improvement in precision oncology implementation in Japan2022

    • Author(s)
      Sunami K, Naito Y, Komine K, Amano T, Ennishi D, Imai M, Kage H, Kanai M, Kenmotsu H, Koyama T, Maeda T, Morita S, Sakai D, Kohsaka S, Tsuchihara K, Saigusa Y, Yoshino T
    • Journal Title

      Cancer Sci

      Volume: 113 Issue: 11 Pages: 3995-4000

    • DOI

      10.1111/cas.15517

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A multicenter investigator-initiated Phase 2 trial of E7090 in patients with advanced or recurrent solid tumor with fibroblast growth factor receptor (FGFR) gene alteration: FORTUNE trial2022

    • Author(s)
      Chiba Y, Sudo K, Kojima Y, Okuma H, Kohsaka S, Machida R, Ichimura M, Anjo K, Kurishita K, Okita N, Nakamura K, Kinoshita I, Takahashi M, Matsubara J, Kusaba H, Yonemori K, Takahashi M
    • Journal Title

      BMC Cancer

      Volume: 22 Issue: 1 Pages: 869-869

    • DOI

      10.1186/s12885-022-09949-8

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Recommendations related to the analytical equivalence assessment of gene panel testing2022

    • Author(s)
      Nagai Sumimasa、Nishihara Hiroshi、Suzuki Takayoshi、Nishio Kazuto、Taniguchi Hiroya、Tsuchihara Katsuya、Nakamura Kohei、Takamatsu Reika、Ueno Toshihide、Aburatani Hiroyuki、Kohno Takashi、Kohsaka Shinji
    • Journal Title

      Cancer Science

      Volume: 113 Issue: 10 Pages: 3282-3290

    • DOI

      10.1111/cas.15513

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Expert panel consensus recommendations on the use of circulating tumor DNA assays for patients with advanced solid tumors2022

    • Author(s)
      Imai M, Nakamura Y, Sunami K, Kage H, Komine K, Koyama T, Amano T, Ennishi D, Kanai M, Kenmotsu H, Maeda T, Morita S, Sakai D, Bando H, Makiyama A, Suzuki T, Hirata M, Kohsaka S, Tsuchihara K, Naito Y, Yoshino T
    • Journal Title

      Cancer Sci

      Volume: 113 Issue: 11 Pages: 3646-3656

    • DOI

      10.1111/cas.15504

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Feasibility and clinical utility of comprehensive genomic profiling of hematological malignancies2022

    • Author(s)
      Fukuhara S, Oshikawa-Kumade Y, Kohsaka S et al.
    • Journal Title

      Cancer Sci

      Volume: 113 Issue: 8 Pages: 2763-2777

    • DOI

      10.1111/cas.15427

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Clinical relevance of PD-L2 expression in surgically resected lung adenocarcinoma.2022

    • Author(s)
      Takamochi K, Hara K, Hayashi T, Kohsaka S, Takahashi F, Suehara Y, Shimokawa M, Suzuki K.
    • Journal Title

      Lung Cancer

      Volume: 168 Pages: 50-58

    • DOI

      10.1016/j.lungcan.2022.04.011

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Tumor mutational burden measurement using comprehensive genomic profiling assay2022

    • Author(s)
      Kage Hidenori、Kohsaka Shinji、Tatsuno Kenji、Ueno Toshihide、Ikegami Masachika、Zokumasu Koichi、Shinozaki-Ushiku Aya、Nagai Sumimasa、Aburatani Hiroyuki、Mano Hiroyuki、Oda Katsutoshi
    • Journal Title

      Japanese Journal of Clinical Oncology

      Volume: 52 Issue: 8 Pages: 925-929

    • DOI

      10.1093/jjco/hyac063

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic diagnosis of pseudomyxoma peritonei originating from mucinous borderline tumor inside an ovarian teratoma2022

    • Author(s)
      Taguchi Ayumi、Rokutan Hirofumi、Oda Katsutoshi、Tanikawa Michihiro、Tanimoto Saki、Sone Kenbun、Mori Mayuyo、Tsuruga Tetsushi、Kohsaka Shinji、Tatsuno Kenji、Shinozaki-Ushiku Aya、Miyagawa Kiyoshi、Mano Hiroyuki、Aburatani Hiroyuki、Ushiku Tetsuo、Osuga Yutaka
    • Journal Title

      BMC Medical Genomics

      Volume: 15 Issue: 1 Pages: 51-51

    • DOI

      10.1186/s12920-022-01188-x

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Composite Clonal Analysis Reveals Transition of NSCLC Subtypes Through Accumulation of Gene Mutations: A Case Report2022

    • Author(s)
      Ando T, Kage H, Shinozaki-Ushiku A, Tatsuno K, Tsutsumi S, Nagayama K, Nakajima J, Kohsaka S, Miyagawa K, Aburatani H, Mano H, Nagase T.
    • Journal Title

      JTO Clin Res Rep

      Volume: 3 Issue: 2 Pages: 100277-100277

    • DOI

      10.1016/j.jtocrr.2022.100277

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Role of the EZH2 and H3K27me3 Expression as a Predictor of Clinical Outcomes in Salivary Duct Carcinoma Patients: A Large-Series Study With Emphasis on the Relevance to the Combined Androgen Blockade and HER2-Targeted Therapy2022

    • Author(s)
      Saigusa N, Hirai H, Kohsaka S, et al.
    • Journal Title

      Front Oncol

      Volume: 11 Pages: 779882-779882

    • DOI

      10.3389/fonc.2021.779882

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] CIC-Mediated Modulation of MAPK Signaling Opposes Receptor Tyrosine Kinase Inhibitor Response in Kinase-Addicted Sarcoma2022

    • Author(s)
      Odintsov I, Ortiz MV, Khodos I, Mattar MS, Lui AJW, Kohsaka S, de Stanchina E, Bender JLG, Ladanyi M, Somwar R.
    • Journal Title

      Cancer Res

      Volume: 82 Issue: 6 Pages: 1110-1127

    • DOI

      10.1158/0008-5472.can-21-1397

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Distinct properties of pure- and mixed-type high-grade fetal lung adenocarcinomas by genetic profiling and transcription factor expression.2022

    • Author(s)
      Kishikawa S, Hayashi T, Saito T, Takamochi K, Sasa K, Suehara Y, Takahashi F, Sasahara N, Kohsaka S, Suzuki K, Yao T
    • Journal Title

      Virchows Arch

      Volume: 480 Issue: 3 Pages: 609-619

    • DOI

      10.1007/s00428-021-03247-7

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preclinical assessment of combination therapy of EGFR tyrosine kinase inhibitors in a highly heterogeneous tumor model2022

    • Author(s)
      Ikeuchi H, Hirose T, Ikegami M, Takamochi K, Suzuki K, Mano H, Kohsaka S.
    • Journal Title

      Oncogene

      Volume: 41 Issue: 17 Pages: 2470-2479

    • DOI

      10.1038/s41388-022-02263-4

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] MicroSEC; Sequence error filtering pipeline for formalin-fixed and paraffin-embedded samples.2021

    • Author(s)
      Ikegami M, Kohsaka S, Hirose T, Ueno T, Inoue S, Konomata N, Yamauchi H, Mori T, Sekine S, Inamoto Y, Yatabe Y, Kobayashi H, Tanaka S, Mano H.
    • Journal Title

      Commun Biol

      Volume: 4 Issue: 1 Pages: 1396-1396

    • DOI

      10.1038/s42003-021-02930-4

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comparative Study on the Efficacy and Exposure of Molecular Target Agents in Non?small Cell Lung Cancer PDX Models with Driver Genetic Alterations2021

    • Author(s)
      Jo Hitomi、Yagishita Shigehiro、Hayashi Yoshiharu、Ryu Shoraku、Suzuki Mikiko、Kohsaka Shinji、Ueno Toshihide、Matsumoto Yuji、Horinouchi Hidehito、Ohe Yuichiro、Watanabe Shun-ichi、Motoi Noriko、Yatabe Yasushi、Mano Hiroyuki、Takahashi Kazuhisa、Hamada Akinobu
    • Journal Title

      Molecular Cancer Therapeutics

      Volume: 21 Issue: 2 Pages: 359-370

    • DOI

      10.1158/1535-7163.mct-21-0371

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Scapular Angiomatoid Fibrous Histiocytoma with EWSR1-CREB1 Fusion in an Adult Patient2021

    • Author(s)
      Kobayashi H, Makise N, Shinozaki-Ushiku A, Ishibashi Y, Ikegami M, Kohsaka S, Ushiku T, Oda K, Miyagawa K, Aburatani H, Mano H, Tanaka S.
    • Journal Title

      Case Rep Orthop

      Volume: 2021 Pages: 9434222-9434222

    • DOI

      10.1155/2021/9434222

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Programmed death-ligand 1 expression and its associations with clinicopathological features, prognosis, and driver oncogene alterations in surgically resected lung adenocarcinoma2021

    • Author(s)
      Takamochi K, Hara K, Hayashi T, Kohsaka S, Takahashi F, Suehara Y, Suzuki K.
    • Journal Title

      Lung Cancer

      Volume: 161 Pages: 163-170

    • DOI

      10.1016/j.lungcan.2021.09.011

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Highly sensitive fusion detection using plasma cell-free RNA in non-small-cell lung cancers.2021

    • Author(s)
      Hasegawa N, Kohsaka S, Kurokawa K, Shinno Y, Takeda Nakamura I, Ueno T, Kojima S, Kawazu M, Suehara Y, Ishijima M, Goto Y, Kojima Y, Yonemori K, Hayashi T, Saito T, Shukuya T, Takahashi F, Takahashi K, Mano H.
    • Journal Title

      Cancer Sci

      Volume: 112 Issue: 10 Pages: 4393-4403

    • DOI

      10.1111/cas.15084

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comprehensive functional evaluation of variants of fibroblast growth factor receptor genes in cancer.2021

    • Author(s)
      Takeda Nakamura I, Kohsaka S, Ikegami M, Ueno T, Li K, Beyett T, Koyama T, Shimizu T, Yamamoto N, Takahashi F, Takahashi K, Eck M, Mano H.
    • Journal Title

      NPJ Precision Oncology

      Volume: 16 Issue: 1 Pages: 66-66

    • DOI

      10.1038/s41698-021-00204-0

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Comprehensive molecular profiling of pulmonary pleomorphic carcinoma2021

    • Author(s)
      Nagano Masaaki、Kohsaka Shinji、Hayashi Takuo、Ueno Toshihide、Kojima Shinya、Shinozaki-Ushiku Aya、Morita Shigeki、Tsuda Masumi、Tanaka Shinya、Shinohara Toshiya、Omori Yuko、Sugaya Fumiko、Kato Hiroaki、Narita Yoshiaki、Nakajima Jun、Suzuki Kenji、Takamochi Kazuya、Mano Hiroyuki
    • Journal Title

      npj Precision Oncology

      Volume: 5 Issue: 1 Pages: 57-57

    • DOI

      10.1038/s41698-021-00201-3

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Appropriate use of cancer comprehensive genome profiling assay using circulating tumor DNA.2021

    • Author(s)
      Sunami K, Bando H, Yatabe Y, Naito Y, Takahashi H, Tsuchihara K, Toyooka S, Mimori K, Kohsaka S, Uetake H, Kinoshita I, Komine K, Takeda M, Hayashida T, Tamura K, Nishio K, Yamamoto N; Working Group of a Joint Task Force of Three Academic Societies for the Promotion of Cancer Genomic Medicine.
    • Journal Title

      Cancer Sci.

      Volume: 112(9) Issue: 9 Pages: 3911-3917

    • DOI

      10.1111/cas.15022

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Transcription start site-level expression of thyroid transcription factor 1 isoforms in lung adenocarcinoma and its clinicopathological significance.2021

    • Author(s)
      Sano K, Hayashi T, Suehara Y, Hosoya M, Takamochi K, Kohsaka S, Kishikawa S, Kishi M, Saito S, Takahashi F, Kaneko K, Suzuki K, Yao T, Ishijima M, Saito T.
    • Journal Title

      J Pathol Clin Res

      Volume: 7 Issue: 4 Pages: 361-374

    • DOI

      10.1002/cjp2.213

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Correction to: The initial assessment of expert panel performance in core hospitals for cancer genomic medicine in Japan2021

    • Author(s)
      Sunami K, Naito Y, Aimono E, Amano T, Ennishi D, Kage H, Kanai M, Komine K, Koyama T, Maeda T, Morita S, Sakai D, Kohsaka S, Tsuchihara K, Yoshino T.
    • Journal Title

      Int J Clin Oncol

      Volume: 26 Issue: 5 Pages: 1007-1007

    • DOI

      10.1007/s10147-021-01897-w

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of an optimal protocol for molecular profiling of tumor cells in pleural effusions at single-cell level2021

    • Author(s)
      Takeda Nakamura I, Ikegami M, Hasegawa N, Hayashi T, Ueno T, Kawazu M, Yagishita S, Goto Y, Shinno Y, Kojima Y, Takamochi K, Takahashi F, Takahashi K, Mano H, Kohsaka S
    • Journal Title

      Cancer Sci

      Volume: - Issue: 5 Pages: 2006-2019

    • DOI

      10.1111/cas.14821

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 大規模データ解析が拓くがんゲノム医療のNext stage2023

    • Author(s)
      高阪 真路
    • Organizer
      第113 回日本病理学会総会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 肺腺癌におけるRNA解析の臨床的妥当性・有用性の検討2023

    • Author(s)
      寺島 祐樹、池内 洋史、朴 秀煥、上野 敏秀、小島 進也、高持 一矢、高阪 真路
    • Organizer
      第113 回日本病理学会総会
    • Related Report
      2023 Annual Research Report
  • [Presentation] TOP-Lパネルの開発2023

    • Author(s)
      高阪 真路
    • Organizer
      第8回Liquid Biopsy 研究会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 日本国内固形癌患者における NTRK 融合遺伝子の保有率を調査する後方視的観察研究2023

    • Author(s)
      高阪 真路
    • Organizer
      第61回日本癌治療学会学術総会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 次世代 CGP 検査の幕開け―GenMineTOP への期待―2023

    • Author(s)
      高阪 真路
    • Organizer
      第61回日本癌治療学会学術総会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Opening a new era of omics-based medicine2023

    • Author(s)
      高阪 真路
    • Organizer
      第14 回 国際ゲノム会議
    • Related Report
      2023 Annual Research Report
  • [Presentation] Liquid Biopsy from bench to bed side2023

    • Author(s)
      高阪 真路
    • Organizer
      第82日本癌学会学術総会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 次世代CGP検査が拓く新たながんゲノム医療2023

    • Author(s)
      高阪 真路
    • Organizer
      第82日本癌学会学術総会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 次世代CGP検査の幕開け2023

    • Author(s)
      高阪 真路
    • Organizer
      第82日本癌学会学術総会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 膵癌患者の組織とリキッドバイオプシー検体を用いた分子プロファイリングの有用性2023

    • Author(s)
      柳垣 充、宇和川 匡、上野 敏秀、今井 健太、高橋 数冴、新井田厚司、恒松 雅、白井 祥睦、春木 孝一郎、古川 賢英、後町 武志、池上 徹、間野 博行、高阪 真路
    • Organizer
      第82日本癌学会学術総会
    • Related Report
      2023 Annual Research Report
  • [Presentation] J-PDX ライブラリーにおける Short Tandem Repeat 解析による品質管理の有用性検討2023

    • Author(s)
      吉野 友美、柳下 薫寛、牛江 千明、大藤 千里、高橋 亜子、高阪 真路、上野 敏秀、濱田 哲暢
    • Organizer
      第82日本癌学会学術総会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 我が国におけるがんゲノム医療の現状と展望2023

    • Author(s)
      高阪真路
    • Organizer
      日本頭頸部外科学会第12回教育セミナー
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] ゲノム研究で新しいがん治療法を創る2023

    • Author(s)
      高阪真路
    • Organizer
      WCRW2023
    • Related Report
      2023 Annual Research Report
  • [Presentation] ゲノム研究で新しいがん治療法を創る2023

    • Author(s)
      高阪真路
    • Organizer
      第40回分子病理学研究会 クラークシンポジウム
    • Related Report
      2023 Annual Research Report
  • [Presentation] 日米におけるがん遺伝子プロファイリング検査開発の展望2023

    • Author(s)
      高阪真路
    • Organizer
      第56回日本整形外科学会骨・軟部腫瘍学術集会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] がんゲノム研究が拓く新しい医療2023

    • Author(s)
      高阪真路
    • Organizer
      第29回日本遺伝性腫瘍学会学術集会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] 我が国におけるがんゲノム医療の現状と展望2023

    • Author(s)
      高阪真路
    • Organizer
      第35回日本内分泌外科学会総会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] Exploratory novel biomarker and resistance mechanism of milademetan, an MDM2 inhibitor, in amplified MDM2 intimal sarcoma from an open-label phase 1b/2 trial (NCCH1806/MK004).2023

    • Author(s)
      Takafumi Koyama、Toshio Shimizu、Yuki Kojima、Kazuki Sudo、Hitomi Sumiyoshi Okuma、Tatsunori Shimoi、Hitoshi Ichikawa、Shinji Kohsaka、Akihiko Yoshida、Naoko Matsui、Kenichi Nakamura、Noboru Yamamoto、Kan Yonemori
    • Organizer
      2023 ASCO Annual Meeting
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] RNAシークエンスが拓く新たながんゲノム医療と将来展望2023

    • Author(s)
      高阪真路
    • Organizer
      第112回日本病理学会総会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Development of a comprehensive assay for the molecular profiling of cancer by target enrichment from FFPE specimens2023

    • Author(s)
      Shinji Kohsaka
    • Organizer
      2023 Joint Winter Meeting with RSM
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] RNA解析が拓く新たながんゲノム医療2022

    • Author(s)
      高阪真路
    • Organizer
      第60回日本癌治療学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 東大オンコパネル(TOP)を用いたプレシジョンメディシン(先進医療 B)2022

    • Author(s)
      鹿毛 秀宣、牛久 綾、石垣 和祥、佐藤 悠佑、田辺 真彦、田中 將太、谷川 道洋、渡邊 広祐、高阪 真路、辰野 健二、牛久 哲男、油谷 浩幸、間野 博行、宮川 清、織田 克利
    • Organizer
      第81日本癌学会学術総会
    • Related Report
      2022 Annual Research Report
  • [Presentation] FGFR 遺伝子変異のがん化能および薬剤感受性に対する網羅的解析2022

    • Author(s)
      中村 育子、高阪 真路、上野 敏秀、高橋 史行、高橋 和久、間野博行
    • Organizer
      第81日本癌学会学術総会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 網羅的薬効予測マーカー探索プラットフォームの構築2022

    • Author(s)
      池内 洋史、松野 悠介、池上 政周、小島 進也、大熊 ひとみ、小島 勇貴、米盛 勧、谷田部 恭、吉岡 研一、間野 博行、高阪 真路
    • Organizer
      第81日本癌学会学術総会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 小児固形がんにおける循環腫瘍 DNA を用いたリキッドバイオプシーの臨床的有用性の検討2022

    • Author(s)
      柳垣 充、荒川 歩、上野 敏秀、大熊 ひとみ、池上 徹、間野 博行、高阪 真路
    • Organizer
      第81日本癌学会学術総会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 遺伝子プロファイリングを目指した Ghost Cytometry によるcirculating tumor cell 分取実験2022

    • Author(s)
      伊田 英恵、河村 踊子、長谷川 延彦、上野 敏秀、小山 隆文、米盛 勧、大江 裕一郎、山本 昇、太田 禎生、間野 博行、高阪 真路
    • Organizer
      第81日本癌学会学術総会
    • Related Report
      2022 Annual Research Report
  • [Presentation] ユーイング肉腫における診断・治療モニタリングツールとしての血漿中の cell-free RNA の可能性2022

    • Author(s)
      長谷川 延彦、窪田 大介、高木 辰哉、齋藤 剛、岩田 慎太郎、川井 章、高阪 真路、間野 博行
    • Organizer
      第81日本癌学会学術総会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 人工微小環境ポリマーアレイによる新規セルフェノミクスの創出とがん層別化法の開発2022

    • Author(s)
      椨 康一、高阪 真路、秀 拓一郎、田賀 哲也
    • Organizer
      第81日本癌学会学術総会
    • Related Report
      2022 Annual Research Report
  • [Presentation] がんゲノム医療におけるRNAシークエンスの活用法2022

    • Author(s)
      高阪真路
    • Organizer
      第81日本癌学会学術総会
    • Related Report
      2022 Annual Research Report
  • [Presentation] シングルセル解析の臨床応用2022

    • Author(s)
      高阪真路
    • Organizer
      第7回クリニカルバイオバンク学会シンポジウム
    • Related Report
      2022 Annual Research Report
  • [Presentation] がんゲノム医療が加速する研究開発2022

    • Author(s)
      高阪真路
    • Organizer
      第73回日本電気泳動学会総会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] リキッドバイオプシーの実践2022

    • Author(s)
      高阪真路
    • Organizer
      第19回日本婦人科がん会議
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] がん遺伝子プロファイリング検査におけるリキッドバイオプシーの最適化2022

    • Author(s)
      高阪真路
    • Organizer
      第 63 回日本臨床細胞学会総会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 循環腫瘍細胞を用いた遺伝子プロファイリング検査の構築2022

    • Author(s)
      高阪真路
    • Organizer
      第 63 回日本臨床細胞学会総会
    • Related Report
      2022 Annual Research Report
  • [Presentation] BRCA機能的スクリーニングとHBOC診療の個別化2022

    • Author(s)
      高阪真路
    • Organizer
      第 2 回 JOHBOC 学術総会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] がんゲノム医療におけるリキッドバイオプシーの有用性と現状2021

    • Author(s)
      高阪真路
    • Organizer
      第80日本癌学会学術総会
    • Related Report
      2021 Annual Research Report
  • [Presentation] High throughput functional analysis for the clinical annotation in cancer genome medicine2021

    • Author(s)
      Shinji Kohsaka
    • Organizer
      1stJCA-AACR Precision Cancer Medicine International Conference
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] がんゲノム医療におけるリキッドバイオプシーの有用性と現状2021

    • Author(s)
      高阪真路
    • Organizer
      第62回日本臨床細胞学会総会春期大会
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Book] がんゲノム病理学2021

    • Author(s)
      高阪真路
    • Total Pages
      234
    • Publisher
      文光堂
    • ISBN
      9784830604850
    • Related Report
      2021 Annual Research Report

URL: 

Published: 2021-04-28   Modified: 2025-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi