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Programming somitogenesis with synthetic cell-cell signaling

Research Project

Project/Area Number 21H03540
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 62010:Life, health and medical informatics-related
Research InstitutionKyoto University

Principal Investigator

Isomura Akihiro  京都大学, 医生物学研究所, 特定准教授 (70512466)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2023: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2022: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2021: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Keywords合成生物学 / 人工遺伝子回路 / オルガノイド / 分節時計 / 光遺伝学
Outline of Research at the Start

本研究では、細胞自律的または非自律的に働く人工遺伝子回路を構築し、その動的特性を遺伝子発現の光制御・光計測技術によって定量的に測定・評価する。そして、体節組織形成に必要な遺伝子回路を破壊した変異細胞集団の組織構築を、人工遺伝子回路によって回復させることを試みる。これらの再構成実験によって 人工遺伝子回路の機能性を実証し、哺乳動物細胞の3次元組織構築を操作する技術基盤を確立する。

Outline of Final Research Achievements

The objective of this research was to establish a fundamental technology for quantitatively measuring and evaluating the dynamic characteristics of synthetic gene circuits to demonstrate the functionality in morphogenesis. The dynamic properties of the newly developed synthetic signaling pathway were further clarified, which further applied to the manipulation of the segmentation clock in mutant organoids.

Academic Significance and Societal Importance of the Research Achievements

本研究によって、効率的な動的情報伝達を可能とする人工リガンド・人工受容体の選別・機能評価プラットフォームが、オルガノイド技術の導入を経て深化した。この技術の更なる進展によって、哺乳動物細胞の組織構築を人工遺伝子回路を使って制御・再構築することが可能になり、再生医療に資する人工多細胞組織の作製などの応用に繋がることが期待される。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • Research Products

    (4 results)

All 2023

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (2 results) (of which Invited: 1 results)

  • [Journal Article] Differential cell-cycle control by oscillatory versus sustained Hes1 expression via p212023

    • Author(s)
      Maeda Yuki、Isomura Akihiro、Masaki Taimu、Kageyama Ryoichiro
    • Journal Title

      Cell Reports

      Volume: 42 Issue: 5 Pages: 112520-112520

    • DOI

      10.1016/j.celrep.2023.112520

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Biological Significance of the Coupling Delay in Synchronized Oscillations2023

    • Author(s)
      Kageyama Ryoichiro、Isomura Akihiro、Shimojo Hiromi
    • Journal Title

      Physiology

      Volume: 38 Issue: 2 Pages: 63-72

    • DOI

      10.1152/physiol.00023.2022

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 分節時計における遺伝子発現振動波の制御機構2023

    • Author(s)
      磯村彰宏
    • Organizer
      第75 回日本細胞生物学会大会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] 人工遺伝子回路による分節時計の再構成と操作2023

    • Author(s)
      磯村彰宏
    • Organizer
      第46 回日本分子生物学会大会
    • Related Report
      2023 Annual Research Report

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Published: 2021-04-28   Modified: 2025-01-30  

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