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Development of ISFET array sensor for pH measurement of pore water in marine sediment

Research Project

Project/Area Number 21H03578
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 63010:Environmental dynamic analysis-related
Research InstitutionTokyo Metropolitan University

Principal Investigator

Nakajima Hizuru  東京都立大学, 都市環境科学研究科, 准教授 (10432858)

Co-Investigator(Kenkyū-buntansha) 茅根 創  東京大学, 大学院理学系研究科(理学部), 教授 (60192548)
森岡 和大  東京薬科大学, 薬学部, 助教 (70794056)
Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2023: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2022: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2021: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
KeywordspHセンサー / イオン感応性電界効果トランジスタ / ISFET / 海洋酸性化 / センサー / pH
Outline of Research at the Start

海洋酸性化の影響が顕著である海底堆積物中の間隙水のpHをモニタリングすることは,海洋酸性化の進行度を評価する上で極めて需要である。しかし,海底堆積物中の間隙水のpHを連続的に計測する技術は未だ確立されていない。そこで本研究では,微小な拡張ゲート電極をアレイ化したイオン感応性電界効果トランジスタ(ISFET)センサーを開発し,これを海底堆積物中に設置することにより,周囲の環境を維持した状態で,海底堆積物中の間隙水の深度方向のpHを連続的に計測する技術を開発する。

Outline of Final Research Achievements

If the pH profile of porewater in marine sediments can be measured, the progress of ocean acidification and CO2 absorption by dissolution of calcium carbonate can be evaluated. However, analytical techniques that can continuously measure the pH profile of pore water in marine sediments have not yet been established. In this study, we developed an ion-sensitive field-effect transistor (ISFET) sensor with an array of small extended gate electrodes and succeeded in continuous measurement of the pH profile of pore water in marine sediments using the developed sensor. It was found that the pH of seawater was about 0.1-0.3 higher than that of pore water in marine sediments, and that the pH of seawater during the night was about 0.3 lower than that during the day.

Academic Significance and Societal Importance of the Research Achievements

本研究で開発したISFETアレイセンサーは,海底堆積物中の間隙水のpHプロファイルを高い分解能で連続的に計測することができるセンサーである。したがって,本センサーが実用化されれば,「海底堆積物中の間隙水のpHプロファイルが短期あるいは長期的にどのように変動するか?」という未知の事象が明らかになり,サンゴ礁の砂地に棲息する海洋生物に対する酸性化の影響や,砂地の溶解速度などに関する新たな知見が得られる。したがって,本研究で開発したセンサーは,海洋での計測技術を飛躍的に発展させるとともに,海洋酸性化に関する研究の進歩に大きく貢献する可能性が高いと考えられる。

Report

(3 results)
  • 2023 Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • Research Products

    (38 results)

All 2023 2022 2021

All Journal Article (19 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 19 results,  Open Access: 3 results) Presentation (16 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] Hydrophilic interaction chromatography-type sorbent prepared by the modification of methacrylate-base resin with polyethyleneimine for solid-phase extraction of polar compounds2023

    • Author(s)
      Hiroya Murakami, Keisuke Iida, Yuki Oda, Tomonari Umemura, Hizuru Nakajima, Yukihiro Esaka, Yoshinori Inoue, Norio Teshima
    • Journal Title

      Analytical Sciences

      Volume: 39 Pages: 375-381

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of Portable Fluorescence Microplate Reader Equipped with Indium Tin Oxide Glass Heater for Loop-mediated Isothermal Amplification2022

    • Author(s)
      Ryo Ishii, Kazuhiro Morioka, Takuya Mizumoto, Natsumi Yamasaki, Akihide Hemmi, Atsushi Shoji, Hiroya Murakami, Norio Teshima, Tomonari Umemura, Katsumi Uchiyama, Hizuru Nakajima
    • Journal Title

      Sensors and Materials

      Volume: 34 Issue: 3 Pages: 971

    • DOI

      10.18494/SAM.2022.3618

    • ISSN
      0914-4935, 2435-0869
    • Year and Date
      2022-03-03
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A Measurement Method for Cytochrome P450 3A4 (CYP3A4)-mediated Oxidation of Cholesterol in Lipid Membranes2022

    • Author(s)
      A. Shoji, H. Miki, M. Kikkawa, M. Yamamoto, Y. Moriiwa, K. Morioka, A. Yanagida
    • Journal Title

      Sensors and Materials

      Volume: 34 Issue: 3 Pages: 951

    • DOI

      10.18494/SAM3598

    • ISSN
      0914-4935, 2435-0869
    • Year and Date
      2022-03-03
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a Rapid Screening Method for Detecting Drugs-metal Ions Interaction Using Ion Selective Electrode2022

    • Author(s)
      守岩友紀子,木村ももこ,小田彩夏,森岡和大,東海林敦,柳田顕郎
    • Journal Title

      BUNSEKI KAGAKU

      Volume: 71 Issue: 1.2 Pages: 69-75

    • DOI

      10.2116/bunsekikagaku.71.69

    • ISSN
      0525-1931
    • Year and Date
      2022-01-05
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Localized hydrodynamic flow confinement assisted nanowire sensor for ultrasensitive protein detection2022

    • Author(s)
      Lin Haifeng、Kasai Nahoko、Xu Ning、Nakajima Hizuru、Kato Shungo、Zeng Hulie、Lin Jin-Ming、Mao Sifeng、Uchiyama Katsumi
    • Journal Title

      Biosensors and Bioelectronics

      Volume: 218 Pages: 114788-114788

    • DOI

      10.1016/j.bios.2022.114788

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Microsphere amplified fluorescence and its application in sensing2022

    • Author(s)
      Si Shuhui、Kaneko Tsuguhiro、Xu Lingrui、Luo Huan、Nakajima Hizuru、Kasai Nahoko、Uchiyama Katsumi、Wu Danhong、Zeng Hulie
    • Journal Title

      Biosensors and Bioelectronics

      Volume: 218 Pages: 114791-114791

    • DOI

      10.1016/j.bios.2022.114791

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Regioselective fabrication of gold nanowires using open-space laminar flow for attomolar protein detection2022

    • Author(s)
      Nishitani Yuki、Kasai Nahoko、Nakajima Hizuru、Kato Shungo、Mao Sifeng、Uchiyama Katsumi
    • Journal Title

      Chemical Communications

      Volume: 58 Issue: 27 Pages: 4308-4311

    • DOI

      10.1039/d2cc00507g

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of a surface plasmon resonance sensor using an optical fiber prepared by electroless displacement gold plating and its application to immunoassay2022

    • Author(s)
      Atsushi Shoji, Miyu Nakajima, Kazuhiro Morioka, Eiji Fujimori, Tomonari Umemura, Akio Yanagida, Akihide Hemmi, Katsumi Uchiyama, Hizuru Nakajima
    • Journal Title

      Talanta

      Volume: 240 Pages: 123162-123162

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a colorimetric assay for quantification of favipiravir in human serum using ferrihydrite2022

    • Author(s)
      Yukiko Moriiwa, Natsu Oyama, Ryo Otsuka, Kazuhiro Morioka, Atsushi Shoji, Akio Yanagida
    • Journal Title

      Talanta

      Volume: 252 Pages: 123827-123827

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Trajectory to local extinction of an isolated dugong population near Okinawa Island, Japan2022

    • Author(s)
      Hajime Kayanne, Takeshi Hara, Nobuaki Arai, Hiroya Yamano, Hiroyuki Matsuda
    • Journal Title

      SCIENTIFIC REPORTS

      Volume: 12 Issue: 1 Pages: 6151-6151

    • DOI

      10.1038/s41598-022-09992-2

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a fluorescence microplate reader using an organic photodiode array with a large light receiving area2022

    • Author(s)
      Morioka Kazuhiro、Osashima Moeko、Azuma Nao、Qu Kuizhi、Hemmi Akihide、Shoji Atsushi、Murakami Hiroya、Teshima Norio、Umemura Tomonari、Uchiyama Katsumi、Nakajima Hizuru
    • Journal Title

      Talanta

      Volume: 238 Pages: 122994-122994

    • DOI

      10.1016/j.talanta.2021.122994

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a Simple ELISA System Using a Jungle Gym Structure as an Antibody-Immobilization Substrate2021

    • Author(s)
      熊谷直也, 森岡和大, 中村好花, 千明大悟, 北谷菜津美, 加藤祐史, 東海林敦
    • Journal Title

      BUNSEKI KAGAKU

      Volume: 70 Issue: 12 Pages: 721-728

    • DOI

      10.2116/bunsekikagaku.70.721

    • NAID

      130008162172

    • ISSN
      0525-1931
    • Year and Date
      2021-12-05
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Measurements of Carbonate System in the Ocean2021

    • Author(s)
      茅根創,山本将史,朝海敏昭
    • Journal Title

      BUNSEKI KAGAKU

      Volume: 70 Issue: 6 Pages: 301-308

    • DOI

      10.2116/bunsekikagaku.70.301

    • NAID

      130008068563

    • ISSN
      0525-1931
    • Year and Date
      2021-06-05
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Film-Thickness-Controllable System for Preparing Silver Nanofilms through Absorbance Monitoring of the Thickness during a Silver-Mirror Reaction2021

    • Author(s)
      K. Morita, K. Morioka, H. Nakajima, K. Uchiyama, A. Yanagida, A. Shoji
    • Journal Title

      Analytical Sciences

      Volume: 37 Issue: 4 Pages: 625-631

    • DOI

      10.2116/analsci.20P400

    • NAID

      130008023547

    • ISSN
      0910-6340, 1348-2246
    • Year and Date
      2021-04-10
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of an automated transportable continuous system to measure the total alkalinity of seawater2021

    • Author(s)
      Yamamoto Shoji、Kayanne Hajime、Fujita Nori、Sato Yukari、Kurihara Haruko、Harii Saki、Hemmi Akihide、Dickson Andrew G.
    • Journal Title

      Talanta

      Volume: 221 Pages: 121666-121666

    • DOI

      10.1016/j.talanta.2020.121666

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] マイクロ化学分析システム(μTAS)2021

    • Author(s)
      森岡和大, 中嶋秀
    • Journal Title

      ぶんせき

      Volume: 557 Pages: 208-214

    • NAID

      40022647150

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] オンサイトでの定量分析を指向した小型分析装置2021

    • Author(s)
      森岡和大, 中嶋秀
    • Journal Title

      化学と教育

      Volume: 69 Pages: 210-213

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] <i>In Situ</i> Single-Cell Stimulation and Real-Time Electrochemical Detection of Lactate Response Using a Microfluidic Probe2021

    • Author(s)
      Zhou Lin、Kasai Nahoko、Nakajima Hizuru、Kato Shungo、Mao Sifeng、Uchiyama Katsumi
    • Journal Title

      Analytical Chemistry

      Volume: 93 Issue: 24 Pages: 8680-8686

    • DOI

      10.1021/acs.analchem.1c01054

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A simple and efficient approach to sensitize the fluorescence detection to microwell plate2021

    • Author(s)
      X. He, T. Kaneko, H. Luo, H. Cong, H. Nakajima, H. Zeng
    • Journal Title

      Sensors and Actuators B

      Volume: 343 Pages: 130070-130070

    • DOI

      10.1016/j.snb.2021.130070

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] エクソソーム膜タンパク質計測のための人工生体膜チップの作製方法の検討2022

    • Author(s)
      伊藤真奈,東海林敦,森岡和大,辺見彰秀,山本将史,中嶋秀
    • Organizer
      第3回生体膜デザインコンファレンス
    • Related Report
      2022 Annual Research Report
  • [Presentation] 脂質二分子膜に包埋させたグラミシジンのマルチチャネル活性評価2022

    • Author(s)
      伊藤真奈,東海林敦,森岡和大,辺見彰秀,山本将史,中嶋秀
    • Organizer
      日本分析化学会第71年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 4-ノズル化学ペンを用いる単一細胞分化法の開発2022

    • Author(s)
      遠山万理乃,河西奈保子,中嶋秀,加藤俊吾,毛思鋒,内山一美
    • Organizer
      日本分析化学会第71年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] マルチチャンネル型ISFET pHセンサーの開発及び砂地堆積物での鉛直方向pH分布の観測結果2022

    • Author(s)
      小川良太,中嶋秀,山本将史,鈴木拳太,森岡和大,茅根創,武田智子,辺見彰秀
    • Organizer
      日本サンゴ礁学会第25回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] LAMP法を用いる手のひらサイズの遺伝子検査装置の開発2022

    • Author(s)
      廣瀬颯太,石井領,森岡和大,山本将史,中嶋秀
    • Organizer
      令和4年度日本分析化学会関東支部若手交流会
    • Related Report
      2022 Annual Research Report
  • [Presentation] CD型電気化学分析システムの開発とmiRNA分析への応用2022

    • Author(s)
      村野唯人,森岡和大,山本将史,中嶋秀
    • Organizer
      令和4年度日本分析化学会関東支部若手交流会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 2次元SPRセンサーを用いるエクソソーム分析法の開発2022

    • Author(s)
      小山大輝,伊藤真奈,東海林敦,森岡和大,辺見彰秀,山本将史,中嶋秀
    • Organizer
      第4回 生体膜デザインコンファレンス
    • Related Report
      2022 Annual Research Report
  • [Presentation] オンサイト測定を指向したポータブル分析装置の開発2022

    • Author(s)
      石井領,香川雅和,伊藤真奈,山中俊介,吉田貴裕,曲奎智,中嶋秀
    • Organizer
      令和3年度分析イノベーション交流会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 3D プリンティングを基軸とする分析技術・装置の開発2022

    • Author(s)
      森岡和大
    • Organizer
      令和3年度分析イノベーション交流会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] ピペットチップを用いる酵素免疫測定法の開発2021

    • Author(s)
      香川雅和,長嶋萌子,東奈穂,森岡和大,東海林敦,辺見彰秀,村上博哉,手嶋紀雄,梅村知也,内山一美,中嶋秀
    • Organizer
      第57回フローインジェクション分析講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] キャピラリーを用いる表面プラズモン共鳴センサーの開発2021

    • Author(s)
      山中俊介,中島美優,森岡和大,東海林敦,辺見彰秀,村上博哉,手嶋紀雄,梅村知也,内山一美,中嶋秀
    • Organizer
      第57回フローインジェクション分析講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 脂質二分子膜に包埋させたグラミシジンのマルチチャネル活性評価2021

    • Author(s)
      伊藤真奈,森岡和大,辺見彰秀,村上博哉,手嶋紀雄,梅村知也,内山一美,中嶋秀,東海林敦
    • Organizer
      第57回フローインジェクション分析講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] オンチップ流量計測に基づく新規定量分析法の開発2021

    • Author(s)
      曲奎智,森岡和大,東海林敦,辺見彰秀,村上博哉,手嶋紀雄,梅村知也,内山一美,中嶋秀
    • Organizer
      第57回フローインジェクション分析講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] CD型マイクロチップを用いる遺伝子検査のための温度制御方法の検討2021

    • Author(s)
      吉田貴裕,森岡和大,辺見彰秀,東海林敦,村上博哉,手嶋紀雄,梅村知也,内山一美,中嶋秀
    • Organizer
      第57回フローインジェクション分析講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ITOガラスヒーターを用いる携帯型遺伝子検査装置の開発2021

    • Author(s)
      石井領,森岡和,水本拓哉,山崎夏実,辺見彰秀,東海林敦,村上博哉,手嶋紀雄,梅村知也,内山一美,中嶋秀
    • Organizer
      第57回フローインジェクション分析講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] On-chip sample injection system with a small-sized 6-port valve for micro-flow-injection analysis2021

    • Author(s)
      K. Morioka, H. Sato, K. Morita, A. Hemmi, H. Nakajima, A. Shoji, A. Yanagida
    • Organizer
      The International Chemical Congress of Pacific Basin Societies (Pacifichem 2021)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Patent(Industrial Property Rights)] 固体半導体イオンセンサー2021

    • Inventor(s)
      茅根創,辺見彰秀
    • Industrial Property Rights Holder
      茅根創,辺見彰秀
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2021-144727
    • Filing Date
      2021
    • Related Report
      2021 Annual Research Report
  • [Patent(Industrial Property Rights)] マイクロノズル装置2021

    • Inventor(s)
      内山一美,毛思鋒,河西奈保子,周琳,中嶋秀,加藤俊吾
    • Industrial Property Rights Holder
      内山一美,毛思鋒,河西奈保子,周琳,中嶋秀,加藤俊吾
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2021-103735
    • Filing Date
      2021
    • Related Report
      2021 Annual Research Report
  • [Patent(Industrial Property Rights)] マイクロノズル装置2021

    • Inventor(s)
      内山一美,毛思鋒,河西奈保子,林海鋒,中嶋秀,加藤俊吾
    • Industrial Property Rights Holder
      内山一美,毛思鋒,河西奈保子,林海鋒,中嶋秀,加藤俊吾
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2021-137979
    • Filing Date
      2021
    • Related Report
      2021 Annual Research Report

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Published: 2021-04-28   Modified: 2025-01-30  

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