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Assessing the effects of sunscreens on bleaching of hermatypic corals using advanced molecular analysing techniques

Research Project

Project/Area Number 21H03619
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 64010:Environmental load and risk assessment-related
Research InstitutionEhime University

Principal Investigator

TAKEUCHI ICHIRO  愛媛大学, 農学研究科, 教授 (30212020)

Co-Investigator(Kenkyū-buntansha) 治多 伸介  愛媛大学, 農学研究科, 教授 (60218659)
石橋 弘志  愛媛大学, 農学研究科, 准教授 (90403857)
田代 豊  名桜大学, 国際学部, 教授 (20441959)
山城 秀之  琉球大学, 熱帯生物圏研究センター, 名誉教授 (80341676)
Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2023: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2021: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Keywordsサンゴ / 日焼け止め剤 / 白化 / 毒性実験 / 分子生物学的解析 / 網羅的解析 / 網羅的遺伝子発現解析 / 毒性評価実験 / 毒性影響評価
Outline of Research at the Start

日焼け止め剤は皮膚ガン防止のために不可欠であるが、日焼け止め剤に含まれる紫外線吸収剤の主成分であるオキシベンゾンは数10 μg/Lレベルでサンゴが白化すること等が報告されている。しかし、近年、同レベルの濃度に曝露してもサンゴは白化しないとの報告もあり、同物質によるサンゴ白化のメカニズムは未解明であるのが現状である。そこで、本研究では、遺伝子発現解析やサンゴ体色の変動解析等により、オキシベンゾン等のサンゴへの毒性影響評価を行う。

Outline of Final Research Achievements

The usage of sunscreens is essential for the prevention of skin cancer. In this study, we established a long-term rearing method for Acropora spp. of hermatypic corals in the laboratory, conducted rearing experiments with oxybenzone and titanium oxide contained in sunscreens on Acropora spp. and analysed coral colour succession and gene expression. Coral body colour did not change after 1-week of exposure to higher concentrations of oxybenzone and titanium oxide than in coastal waters. While the effects of oxybenzone exposure on gene expression in coral and its symbiotic algae were extremely limited, several effects on coral gene expression were observed with titanium oxide exposure.

Academic Significance and Societal Importance of the Research Achievements

本研究により、小型ガラス水槽等を用いたミドリイシ属サンゴの長期飼育方法を確立することができた。日焼け止め剤の各主要成分のミドリイシ属サンゴへの毒性評価実験を実施し、サンゴ及びその共生藻の遺伝子発現等のへ影響評価をおこなった。その結果、特に、オキシベンゾン(日焼け止め剤の主要な紫外線吸収剤)のミドリイシ属サンゴへの影響は極めて限定的であった。本方法によりサンゴ礁域から離れた地域に位置する大学の実験室でもミドリイシ属サンゴを用いた毒性実験が可能になった。よって、サンゴ礁生態系保全に配慮した新たな日焼け止め剤等の開発に貢献することができるであろう。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • Research Products

    (10 results)

All 2024 2023 2022

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (5 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Journal Article] Transcriptome analysis reveals limited toxic effects of the UV-filter benzophenone-3 (BP-3) on the hermatypic coral Acropora tenuis and its symbiotic dinoflagellates2024

    • Author(s)
      Ishibashi H, Nishimura S, Tanaka K, Haruta S, Takayama K, Yamashiro H, Takeuchi I
    • Journal Title

      Marine Pollution Bulletin

      Volume: 201 Pages: 116260-116260

    • DOI

      10.1016/j.marpolbul.2024.116260

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression analyses of stress-responsive genes in the hermatypic coral Acropora tenuis and its symbiotic dinoflagellates after exposure to the herbicide Diuron2023

    • Author(s)
      Ishibashi H, Minamide S, Takeuchi I
    • Journal Title

      Marine Life Science & Technology

      Volume: 5 Issue: 3 Pages: 289-299

    • DOI

      10.1007/s42995-023-00183-0

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of anthropogenic chemicals on hermatypic corals with special reference to gene expression2023

    • Author(s)
      Ishibashi H, Takeuchi I
    • Journal Title

      In, Takeuchi I, Yamashiro H (eds) Coral Reefs of Eastern Asia under Anthropogenic Impacts. Coral Reefs of the World

      Volume: 17 Pages: 153-166

    • DOI

      10.1007/978-3-031-27560-9_9

    • ISBN
      9783031275593, 9783031275609
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anthropogenic stresses in coral reefs and adjacent ecosystems of the East China Sea2023

    • Author(s)
      Takeuchi I
    • Journal Title

      In, Takeuchi I, Yamashiro H (eds) Coral Reefs of Eastern Asia under Anthropogenic Impacts. Coral Reefs of the World

      Volume: 17 Pages: 113-137

    • DOI

      10.1007/978-3-031-27560-9_7

    • ISBN
      9783031275593, 9783031275609
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a compact experimental system for ecotoxicological experiments on Acropora spp.2023

    • Author(s)
      Takeuchi I, Takayama K
    • Journal Title

      In, Takeuchi I, Yamashiro H (eds) Coral Reefs of Eastern Asia under Anthropogenic Impacts. Coral Reefs of the World

      Volume: 17 Pages: 139-151

    • DOI

      10.1007/978-3-031-27560-9_8

    • ISBN
      9783031275593, 9783031275609
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Presentation] Transcriptome analysis of hermatypic coral Acropora tenuis and its symbiotic dinoflagellates exposed to anthropogenic chemicals2024

    • Author(s)
      Takeuchi I, Ishibashi H
    • Organizer
      10th International Conference on Marine Pollution and Ecotoxicology
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 日焼け止め成分オキシベンゾンのミドリイシ属サンゴ/褐虫藻に対する毒性影響2023

    • Author(s)
      石橋弘志・西村紗織里・田中こころ・治多伸介・高山弘太郎・山城秀之・竹内一郎
    • Organizer
      第2回環境化学物質3学会合同大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Transcriptome analysis of the hermatypic coral Acropora tenuis and its symbiotic dinoflagellates exposed to the UV absorber oxybenzone (BP-3) included in sunscreens2023

    • Author(s)
      Ishibashi H, Nishimura S, Tanaka K, Haruta S, Takayama K, Yamashiro H, Takeuchi I
    • Organizer
      SETAC Australasia 2023 Conference
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 除草剤ジウロンを曝露したウスエダミドリイシとその共生藻におけるストレス応答遺伝子の発現解析2023

    • Author(s)
      石橋弘志・南出成梧・竹内一郎
    • Organizer
      日本サンゴ礁学会第26回大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 環境中で検出される日焼け止め成分オキシベンゾンはウスエダミドリイシAcropora tenuis の光合成収率や遺伝子発現に影響するのか?2022

    • Author(s)
      石橋弘志・西村紗織里・田中こころ・治多伸介・高山弘太郎・山城秀之・竹内一郎
    • Organizer
      日本サンゴ礁学会第25回大会
    • Related Report
      2022 Annual Research Report

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Published: 2021-04-28   Modified: 2025-01-30  

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