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Estimation of the relationship between fish communities and environmental transition in inland sea lakes using sediment core DNA

Research Project

Project/Area Number 21H03649
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 64040:Social-ecological systems-related
Research InstitutionShimane University

Principal Investigator

Takahara Teruhiko  島根大学, 学術研究院農生命科学系, 教授 (10536048)

Co-Investigator(Kenkyū-buntansha) 土居 秀幸  京都大学, 情報学研究科, 教授 (80608505)
Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2023: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2022: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2021: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Keywords環境DNA / 堆積物コア / 汽水湖 / 堆積物コアDNA / 環境DNAメタバーコーディング / 魚類相 / 古環境 / 神西湖 / 東郷湖 / 湖山池 / 海跡湖 / 魚類メタバーコーディング / 山陰 / 環境復元 / 種特異的検出 / 宍道湖 / 魚介類 / 水草 / メタバーコーディング / 魚類群集
Outline of Research at the Start

海跡湖の湖底に堆積したコアには、自然変動や社会・経済的な影響による環境変遷の履歴が保存されている。この堆積物コアの情報を読み解くことで、過去の環境変遷に伴って海跡湖がどのような影響を受けてきたのかが明らかになる。本研究では、環境DNA分析(環境中のDNA断片から生物の生息情報を簡便に推定できる技術)を用いて、堆積物コアDNAから高次消費者及び水産有用種を含む魚類群集の復元を行い、復元された魚類群集と環境変遷の関係を解明する。その際、異なる地域性をもつ山陰の海跡湖5つをモデルケースにしてすることで、海跡湖の近過去魚類群集と環境変遷の関係の一般則と地域固有性の両側面を明らかにすることが期待できる。

Outline of Final Research Achievements

Our goal was to clarify the relationship between recent environmental changes and fish communities by analyzing environmental DNA (eDNA) with sediment cores collected from five lagoon lakes in the San'in region (Lakes Shinji, Nakaumi, Jinzai, Togo, and Koyama). eDNA metabarcoding (MB analysis) revealed less fish eDNA in the core samples compared with the water samples in the lakes. Additionally, fish eDNA was detected in relatively deep layers of Lake Nakaumi, in comparison with Lake Shinji and others, suggesting that the preservation of fish eDNA in the sediment cores was influenced by the salinity of each brackish lake. On the other hand, the results of species-specific eDNA analysis showed that plant eDNA was preserved to much deeper cores than fish eDNA. Furthermore, it was suggested that core eDNA derived from aquatic plants in brackish water lakes would be useful in elucidating historical environmental changes.

Academic Significance and Societal Importance of the Research Achievements

海跡湖の堆積物コアから検出できる魚類eDNAは微量であったため、湖の環境変遷を復元する際には水草eDNAも有用であることを明らかにできた学術的意義は大きいと考えている。さらに、水草種の塩分耐性の違いなどに着目することで近過去環境の大規模転換期(例えば、淡水化や汽水化等)などを解明できる可能性も見出すことができた。一方で、海跡湖5つの結果を比較することで、各湖の塩分の高さは堆積物コアにおける魚類eDNAの保存状況に影響している可能性も明らかになってきた。したがって今後は、宍道湖などの塩分が低い海跡湖において、堆積物コアから微量な魚類eDNAを効果的に検出できる手法の開発が必要であると考えられた。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • Research Products

    (34 results)

All 2024 2023 2022 2021

All Journal Article (19 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 16 results,  Open Access: 7 results) Presentation (15 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] 島根県飯南町来島湖における巨大なニホンウナギの発見報告2024

    • Author(s)
      高原輝彦・山岸聖
    • Journal Title

      ホシザキグリーン財団研究報告

      Volume: 27 Pages: 241-244

    • Related Report
      2023 Annual Research Report
  • [Journal Article] Comparing environmental DNA with whole pond survey to estimate the total biomass of fish species in ponds2023

    • Author(s)
      Souma Rio、Katano Izumi、Doi Hideyuki、Takahara Teruhiko、Minamoto Toshifumi
    • Journal Title

      Freshwater Biology

      Volume: 68 Issue: 5 Pages: 727-736

    • DOI

      10.1111/fwb.14059

    • Related Report
      2023 Annual Research Report 2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Species traits and ecosystem characteristics affect species detection by eDNA metabarcoding in lake fish communities2023

    • Author(s)
      Doi Hideyuki、以下20名
    • Journal Title

      Freshwater Biology

      Volume: 68 Issue: 8 Pages: 1346-1358

    • DOI

      10.1111/fwb.14107

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Environmental DNA extraction method from water for a high and consistent DNA yield2023

    • Author(s)
      Fukuzawa Takashi、Shirakura Hiromi、Nishizawa Naofumi、Nagata Hisao、Kameda Yuichi、Doi Hideyuki
    • Journal Title

      Environmental DNA

      Volume: 5 Issue: 4 Pages: 627-633

    • DOI

      10.1002/edn3.406

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sedimentary DNA can reveal the past population dynamics of a pelagic copepod2023

    • Author(s)
      Kai Nakane, Xin Liu, Hideyuki Doi, Gael Dur, Michinobu Kuwae, Syuhei Ban, Narumi Tsugeki
    • Journal Title

      Freshwater Biology

      Volume: - Issue: 11 Pages: 1917-1928

    • DOI

      10.1111/fwb.14096

    • Related Report
      2023 Annual Research Report 2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Discovery of unknown new ponds occupied by the endangered giant water bug Kirkaldyia deyrolli (Hemiptera: Heteroptera: Belostomatidae) by combining environmental DNA and capture surveys2023

    • Author(s)
      Ogata Shigeki、Nishiwaki Atsuhiro、Yamazoe Kanji、Sugai Kyoko、Takahara Teruhiko
    • Journal Title

      Entomological Science

      Volume: 26 Issue: 1

    • DOI

      10.1111/ens.12540

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 環境DNAとDNAバーコーディングを用いたニホンウナギの稚魚期に関する予備的調査2023

    • Author(s)
      山岸聖、吉田真明、福井克也、高原輝彦
    • Journal Title

      ホシザキグリーン財団研究報告

      Volume: 26 Pages: 215-228

    • Related Report
      2022 Annual Research Report
  • [Journal Article] Effective environmental DNA collection for an endangered catfish species: testing for habitat and daily periodicity2022

    • Author(s)
      Takahara Teruhiko、Doi Hideyuki、Kosuge Toshihiro、Nomura Nanae、Maki Nobutaka、Minamoto Toshifumi、Watanabe Katsutoshi
    • Journal Title

      Ichthyological Research

      Volume: - Issue: 4 Pages: 000-000

    • DOI

      10.1007/s10228-022-00900-2

    • Related Report
      2023 Annual Research Report 2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of primer?probe sets for environmental DNA-based monitoring of pond smelt Hypomesus nipponensis and Japanese icefish Salangichthys microdon2022

    • Author(s)
      Takahara Teruhiko、Fukui Katsuya、Hiramatsu Daisuke、Doi Hideyuki、Fujii Masato、Minamoto Toshifumi
    • Journal Title

      Landscape and Ecological Engineering

      Volume: 00 Issue: 1 Pages: 000-000

    • DOI

      10.1007/s11355-022-00507-9

    • Related Report
      2022 Annual Research Report 2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Environmental DNA methods for detecting two invasive alien species (American bullfrog and red swamp crayfish) in Japanese ponds2022

    • Author(s)
      Ogata Shigeki、Doi Hideyuki、Igawa Takeshi、Komaki Shohei、Takahara Teruhiko
    • Journal Title

      Ecological Research

      Volume: 37 Issue: 6 Pages: 701-710

    • DOI

      10.1111/1440-1703.12341

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Filtration extraction method using a microfluidic channel for measuring environmental DNA2022

    • Author(s)
      Fukuzawa Takashi、Kameda Yuichi、Nagata Hisao、Nishizawa Naofumi、Doi Hideyuki
    • Journal Title

      Molecular Ecology Resources

      Volume: 22 Issue: 7 Pages: 2651-2661

    • DOI

      10.1111/1755-0998.13657

    • Related Report
      2022 Annual Research Report
  • [Journal Article] Reconstruction of 100-year dynamics in Daphnia spawning activity revealed by sedimentary DNA.2022

    • Author(s)
      Narumi Tsugeki, Kai Nakane, Hideyuki Doi, Natsuki Ochi and Michinobu Kuwae
    • Journal Title

      Scientific Reports

      Volume: 12 Issue: 1 Pages: 1741-1741

    • DOI

      10.1038/s41598-021-03899-0

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Compilation of real-time PCR conditions toward the standardization of eDNA methods2021

    • Author(s)
      Doi H, Minamoto T, Takahara T, Tsuji S, Uchii K, Yamamoto S, Katano I, Yamanaka H
    • Journal Title

      Ecological Research

      Volume: online first Issue: 3 Pages: 1-10

    • DOI

      10.1111/1440-1703.12217

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Utility of environmental DNA analysis for effective monitoring of invasive fish species in reservoirs.2021

    • Author(s)
      Jo T, Ikeda S, Fukuoka A, Inagawa T, Okitsu J, Katano I, Doi H, Nakai K, Ichiyanagi H, Minamoto T
    • Journal Title

      Ecosphere

      Volume: 12 Issue: 6 Pages: 0000-0000

    • DOI

      10.1002/ecs2.3643

    • NAID

      120007119350

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Simultaneous absolute quantification and sequencing of fish environmental DNA in a mesocosm by quantitative sequencing technique.2021

    • Author(s)
      Hoshino T, Nakao R, Doi H, Minamoto T
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 4372-4372

    • DOI

      10.1038/s41598-021-83318-6

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Degradation modeling of water environmental DNA: Experiments on multiple DNA sources in pond and seawater2021

    • Author(s)
      Saito Tatsuya、Doi Hideyuki
    • Journal Title

      Environmental DNA

      Volume: 3 Issue: 4 Pages: 850-860

    • DOI

      10.1002/edn3.192

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Estimation of biodiversity metrics by environmental DNA metabarcoding compared with visual and capture surveys of river fish communities2021

    • Author(s)
      Doi Hideyuki、Inui Ryutei、Matsuoka Shunsuke、Akamatsu Yoshihisa、Goto Masuji、Kono Takanori
    • Journal Title

      Freshwater Biology

      Volume: ‐ Issue: 7 Pages: 1257-1266

    • DOI

      10.1111/fwb.13714

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] On‐site environmental DNA detection of species using ultrarapid mobile PCR2021

    • Author(s)
      Doi Hideyuki、Watanabe Takeshi、Nishizawa Naofumi、Saito Tatsuya、Nagata Hisao、Kameda Yuichi、Maki Nobutaka、Ikeda Kousuke、Fukuzawa Takashi
    • Journal Title

      Molecular Ecology Resources

      Volume: 21 Issue: 7 Pages: 2364-2368

    • DOI

      10.1111/1755-0998.13448

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Illumina iSeq 100 and MiSeq exhibit similar performance in freshwater fish environmental DNA metabarcoding2021

    • Author(s)
      Nakao Ryohei、Inui Ryutei、Akamatsu Yoshihisa、Goto Masuji、Doi Hideyuki、Matsuoka Shunsuke
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 15763-15763

    • DOI

      10.1038/s41598-021-95360-5

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 環境DNAを用いた宍道湖・中海におけるニホンウナギの中・長期的モニタリングから見えてきたこと2023

    • Author(s)
      永田晃弘・山岸聖・辻井彩花・稲岡悠樹・高原輝彦
    • Organizer
      生物系三学会中国四国地区合同大会(2023年度 徳島大会)
    • Related Report
      2023 Annual Research Report
  • [Presentation] Estimation of paleoenvironment in Lake Shinji through aquatic plant eDNA and CNS analyses using brackish lake sediment cores2023

    • Author(s)
      Satoshi Yamagishi, Koji Seto, Yasuhide Nakamura, Teruhiko Takahara
    • Organizer
      The eDNA Society International Meeting 2023
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 宍道湖堆積物コアを用いた水草DNA解析とCNS元素分析による古環境の推定2023

    • Author(s)
      山岸聖・仲村康秀・瀬戸浩二・高原輝彦
    • Organizer
      島根大学 エスチュアリー研究センター(EsReC) 第30回汽水域研究発表会 汽水域研究会第10回例会 汽水域合同研究発表会2023(ハイブリッド)
    • Related Report
      2022 Annual Research Report
  • [Presentation] 中長期的な環境DNA観測が実現する水草2種の年変動や季節変動の推定2023

    • Author(s)
      高原輝彦・山岸聖 ・下田莉奈・永田晃弘
    • Organizer
      島根大学 エスチュアリー研究センター(EsReC) 第30回汽水域研究発表会 汽水域研究会第10回例会 汽水域合同研究発表会2023(ハイブリッド)
    • Related Report
      2022 Annual Research Report
  • [Presentation] 宍道湖における過去1000年の環境変化と水草の繁茂履歴2023

    • Author(s)
      瀬戸浩二・香月興太・仲村康秀・齋藤文紀・辻本彰・高原輝彦・橋口亜由未・安藤卓人・入月俊明
    • Organizer
      島根大学 エスチュアリー研究センター(EsReC) 第30回汽水域研究発表会 汽水域研究会第10回例会 汽水域合同研究発表会2023(ハイブリッド)
    • Related Report
      2022 Annual Research Report
  • [Presentation] 環境DNAおよびDNA標識を用いたモクズガニ種苗の放流後の追跡調査2022

    • Author(s)
      増成伸文・竹本浩之・草加耕司・野口大毅・山中裕樹・高原輝彦
    • Organizer
      日本甲殻類学会第60回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] DNAメタバーコーディング等を応用した生態系構造の長期的変遷解明2022

    • Author(s)
      仲村康秀・瀬戸浩二・安藤卓人・香月興太・齋藤文紀・小木曽映里・山岸聖・高原輝彦
    • Organizer
      日本海洋学会(2022年度秋季大会)
    • Related Report
      2022 Annual Research Report
  • [Presentation] 堆積物コアDNAを用いた宍道湖におけるマクロ生物の近過去生息状況の推定2022

    • Author(s)
      山岸聖・小室隆・神門利之・引野愛子・坂田雅之・源利文・下田莉奈・高原輝彦
    • Organizer
      生物系三学会中国四国地区合同大会(2022年度島根大会)
    • Related Report
      2022 Annual Research Report
  • [Presentation] 環境DNAを用いた宍道湖・中海におけるニホンウナギの5年間の長期モニタリング2022

    • Author(s)
      辻井彩花・永田晃弘・山岸聖・高原輝彦
    • Organizer
      島根大学エスチュアリー研究センター(EsReC) 第29回汽水域研究発表会 汽水域研究会第10回例会 汽水域合同研究発表会2022
    • Related Report
      2021 Annual Research Report
  • [Presentation] 宍道湖における堆積物コアDNAを指標にした水生動植物の過去の時系列変動の解明2022

    • Author(s)
      山岸聖・下田莉奈・小室隆・神門利之・引野愛子・坂田雅之・源利文・高原輝彦
    • Organizer
      島根大学エスチュアリー研究センター(EsReC) 第29回汽水域研究発表会 汽水域研究会第10回例会 汽水域合同研究発表会2022
    • Related Report
      2021 Annual Research Report
  • [Presentation] Seda DNAを用いた宍道湖における過去の車軸藻類の復元2022

    • Author(s)
      小室隆・神門利之・加藤季晋・引野愛子・山岸聖・高原輝彦・後藤益滋・坂田雅之・源利文
    • Organizer
      2022年日本地理学会春季学術大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 中長期的な環境DNA観測からみえてきた宍道湖・中海の動植物個体群動態2022

    • Author(s)
      高原輝彦
    • Organizer
      3大学連携キックオフシンポジウム(新潟大・金沢大・島根大 環境シンポジウム)
    • Related Report
      2021 Annual Research Report
  • [Presentation] 環境DNAを用いた宍道湖のヤマトシジミとモクズガニの調査事例2021

    • Author(s)
      高原輝彦
    • Organizer
      2021年日本ベントス学会・日本プランクトン学会合同大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 環境DNAを用いたヤマトシジミ資源量推定の試み2021

    • Author(s)
      楠田聡・山﨑哲也・安藤大成・真野修一・神戸崇・荒木仁志・高原輝彦
    • Organizer
      第4回環境DNA学会オンライン大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 環境DNAを用いた宍道湖・中海の流出入河川8本における回遊魚4種の生息実態の推定2021

    • Author(s)
      山岸 聖・土居秀幸・高原輝彦
    • Organizer
      第4回環境DNA学会オンライン大会
    • Related Report
      2021 Annual Research Report

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Published: 2021-04-28   Modified: 2025-01-30  

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