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Molecular dissection of matorisome in a high-regenerative capability animal, salamanders

Research Project

Project/Area Number 21H03829
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 90120:Biomaterials-related
Research InstitutionNational Institute for Basic Biology

Principal Investigator

Suzuki Ken-ichi  基礎生物学研究所, 超階層生物学センター, 特任准教授 (90363043)

Co-Investigator(Kenkyū-buntansha) 遠藤 哲也  愛知学院大学, 教養部, 准教授 (90399816)
Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2023: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2022: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2021: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Keywords器官再生 / 両生類 / 四肢再生 / マトリソーム / ゲノム / オミクス解析 / イモリ / オミクス / ECM / 再生芽 / イベリアトゲイモリ / トランスオミックス / マウス / プロテオミクス
Outline of Research at the Start

本申請課題は、オミックス解析と逆遺伝学的解析が可能となった有尾両生類であるイベリアトゲイモリをモデルとして、高度な器官再生を可能とするマトリソーム因子を徹底的に同定し、器官再生におけるそれらの機能や役割を理解することである。さらに、この再生マトリソームが高等生物に及ぼす効果を評価し、組織工学や再生医療へ革新的な生体材料の開発に繋がる知見を供することを最終目標とする。

Outline of Final Research Achievements

It is believed that regenerating organs contain matrisomes, which are composed of "extracellular matrix" and "secretory growth factors" that ensure cell dedifferentiation and stem cell proliferation. In this study, we performed comparative omics analysis of the matrisomes of newt and frog limb regenerating buds. We knocked out matrisome factors specific to newt regeneration and analyzed their functions in limb regeneration. In addition, we conducted a genome project with the support of the Platform for Advanced Genome Science (PAGS) to improve the protein database required for the advancement of newt matrisome proteomics. In addition, we experimented with transplanting decellularized matrices to induce mouse limb regeneration and evaluated the results.

Academic Significance and Societal Importance of the Research Achievements

本申請課題によって得られる成果は、以下のような先進的な生体材料の開発に繋がることが期待できる。(1)創傷治癒や器官再生を促進する有用な治療用生体材料の開発。(2)より生体に近い機能と構造を持つオルガノイドの創生技術の開発。(3)幹細胞を生体に近い状態で大量に産生可能な培養技術の開発。
生体材料工学に新機軸を打ち出し、イモリの高い器官再生能力についてマトリソームという新しい視点から解明を行う本申請課題は、これまでに前例のないユニークな研究例となることが期待できる。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • Research Products

    (8 results)

All 2024 2023 2022 Other

All Int'l Joint Research (3 results) Journal Article (5 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 4 results)

  • [Int'l Joint Research] California Institute of Technology(米国)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] Karolinska Ins.(スウェーデン)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] MaxPlanck Ins. CBG(ドイツ)

    • Related Report
      2022 Annual Research Report
  • [Journal Article] Fgf10 mutant newts regenerate normal hindlimbs despite severe developmental defects2024

    • Author(s)
      Suzuki M, Okumura A, Chihara A, Shibata Y, Endo T, Teramoto M, Agata K, Bronner ME, Suzuki K
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 121 Issue: 11

    • DOI

      10.1073/pnas.2314911121

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A CRISPR-Cas9-mediated versatile method for targeted integration of a fluorescent protein gene to visualize endogenous gene expression in Xenopus laevis2023

    • Author(s)
      Mochii M, Akizuki K, Ossaka H, Kagawa K, Umesono M, Suzuki K
    • Journal Title

      Developmental Biology

      Volume: 506 Pages: 42-51

    • DOI

      10.1016/j.ydbio.2023.11.010

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CRISPR/Cas9-based simple transgenesis in Xenopus laevis2022

    • Author(s)
      Shibata Y, Suzuki M, Hirose N, Takayama A, Sanbo C, Inoue T, Umesono Y, Agata K, Ueno N, Suzuki K, Mochii M
    • Journal Title

      Developmental Biology

      Volume: 489 Pages: 76-83

    • DOI

      10.1016/j.ydbio.2022.06.001

    • Related Report
      2023 Annual Research Report 2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sequencing and chromosome-scale assembly of the giant Pleurodeles waltl genome2022

    • Author(s)
      Brown T, Elewa A, Iarovenko S, Subramanian E, Araus AJ, Petzold A, Susuki M, Suzuki K, Hayashi T, Toyoda A, Oliveira C, Osipova E, Leigh D, Simon A, Yun M
    • Journal Title

      Biorxiv

      Volume: -

    • DOI

      10.1101/2022.10.19.512763

    • Related Report
      2022 Annual Research Report
    • Open Access / Int'l Joint Research
  • [Journal Article] Newt Hoxa13 has an essential and predominant role in digit formation during development and regeneration2022

    • Author(s)
      Takeuchi Takashi、Matsubara Haruka、Minamitani Fumina、Satoh Yukio、Tozawa Sayo、Moriyama Tomoki、Maruyama Kohei、Suzuki Ken-ichi T.、Shigenobu Shuji、Inoue Takeshi、Tamura Koji、Agata Kiyokazu、Hayashi Toshinori
    • Journal Title

      Development

      Volume: 149 Issue: 5

    • DOI

      10.1242/dev.200282

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access

URL: 

Published: 2021-04-28   Modified: 2025-01-30  

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