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Why can microorganisms withstand drying? Elucidation of drying adaptation mechanism by glass transition of microbial cells

Research Project

Project/Area Number 21H04710
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Review Section Medium-sized Section 38:Agricultural chemistry and related fields
Research InstitutionHokkaido University

Principal Investigator

Koseki Shigenobu  北海道大学, 農学研究院, 教授 (70414498)

Co-Investigator(Kenkyū-buntansha) 川井 清司  広島大学, 統合生命科学研究科(生), 教授 (00454140)
中川 洋  国立研究開発法人日本原子力研究開発機構, 原子力科学研究部門 原子力科学研究所 物質科学研究センター, 研究主幹 (20379598)
Project Period (FY) 2021-04-05 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥42,770,000 (Direct Cost: ¥32,900,000、Indirect Cost: ¥9,870,000)
Fiscal Year 2023: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2022: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2021: ¥20,800,000 (Direct Cost: ¥16,000,000、Indirect Cost: ¥4,800,000)
Keywordsガラス転移 / 乾燥 / 水分活性 / 凍結乾燥 / 含水率 / 適合溶質 / 中性子散乱法 / 乾燥耐性 / 熱分析 / 水分 / 微生物
Outline of Research at the Start

乾燥食品(環境)において微生物が長期間にわたり生残するための乾燥ストレス耐性を獲得するメカニズムを,微生物細胞のガラス転移現象に焦点を絞り,その物理的状態の変化の観点から明らかにする。食中毒細菌の制御から有用細菌の安定保存までを可能とする科学的な基盤を構築することを目的とする。具体的には以下の3つの研究課題について取り組む。
①各種微生物の乾燥ストレス応答に関する統一的解釈を可能とする微生物細胞のガラス化理論を構築する。
②微生物のガラス化に至る過程が乾燥微生物のガラス転移温度に与える影響を解明する。
③乾燥食品中の有害微生物の制御あるいは有用微生物の安定保存のための新たな技術戦略を構築する。

Outline of Final Research Achievements

The glass transition temperature of bacterial cells was affected by the drying method, water activity during storage and drying temperature. Furthermore, by using the difference between the glass transition temperature and the temperature at the time of storage of the bacteria as an indicator, it was clarified that the survival state of bacterial cells placed in a drying environment could be predicted and judged regardless of differences in drying conditions and storage conditions after drying. The analysis of the behaviour of water molecules inside the cell using neutron elastic scattering measurements also revealed that glass transition is not a critical requirement but a trigger when bacteria resume activity from the static state of glass transition due to desiccation.

Academic Significance and Societal Importance of the Research Achievements

これまで乾燥環境下で細菌が長期間にわたり生存し続ける現象は捉えられてきたが、そのメカニズムは不明であった。本研究では細菌が乾燥状態においても長期間にわたり生存し続けることができる要因を、細胞の物理的な変化であるガラス転移現象の観点から明らかにした。乾燥食品の保存温度や湿度を適切に制御することで、仮に有害な食中毒細菌が付着していたとしても、その生存数が予測可能とする技術へと本研究成果は活用されうる。さらに、本研究成果は有用細菌の長期間保存といった面でもガラス転移現象と影響を及ぼす環境因子の制御によって、より長期間にわたり有用細菌を保存可能とするものである。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Comments on the Screening Results   Annual Research Report
  • Research Products

    (11 results)

All 2024 2023 2022 2021

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 2 results) Presentation (5 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results)

  • [Journal Article] Effect of water activity on the mechanical glass transition and dynamical transition of bacteria-solute systems2024

    • Author(s)
      Kawai Kiyoshi、Sogabe Tomochika、Nakagawa Hiroshi、Yamada Takeshi、Koseki Shigenobu
    • Journal Title

      Journal of Food Engineering

      Volume: 375 Pages: 112066-112066

    • DOI

      10.1016/j.jfoodeng.2024.112066

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Incoherent Neutron Scattering and Terahertz Time-Domain Spectroscopy on Protein and Hydration Water2023

    • Author(s)
      Nakagawa Hiroshi、Yamamoto Naoki
    • Journal Title

      Life

      Volume: 13 Issue: 2 Pages: 318-318

    • DOI

      10.3390/life13020318

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of water activity on the mechanical glass transition and dynamical transition of bacteria2022

    • Author(s)
      Sogabe Tomochika、Nakagawa Hiroshi、Yamada Takeshi、Koseki Shigenobu、Kawai Kiyoshi
    • Journal Title

      Biophysical Journal

      Volume: 121 Issue: 20 Pages: 3874-3882

    • DOI

      10.1016/j.bpj.2022.09.001

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Characteristics of Desiccation Tolerant Microorganisms and Its Survival Mechanisms2022

    • Author(s)
      小関 成樹, 川井 清司
    • Journal Title

      Journal of Environmental Biotechnology

      Volume: 22 Issue: 1 Pages: 33-38

    • DOI

      10.50963/jenvbio.22.1_33

    • ISSN
      1347-1856, 2436-5041
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of NaCl on water activity and dynamics of protein hydration water dynamics in proteins by quasi-elastic neutron scattering2022

    • Author(s)
      Hiroshi Nakagawa
    • Journal Title

      Macromolecular Symposia

      Volume: 408 Issue: 1 Pages: 2200071-2200071

    • DOI

      10.1002/masy.202200071

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Why Does Cronobacter sakazakii Survive for a Long Time in Dry Environments? Contribution of the Glass Transition of Dried Bacterial Cells2021

    • Author(s)
      Lee Kyeongmin、Koyama Kento、Kawai Kiyoshi、Koseki Shigenobu
    • Journal Title

      Microbiology Spectrum

      Volume: 9 Issue: 3

    • DOI

      10.1128/spectrum.01384-21

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 水分活性変化による細菌の活動停止-再開とガラス-ラバー転移2023

    • Author(s)
      川丼清司、曽我部知史、中川洋、山田式、小関成樹
    • Organizer
      日本食品工学会第24回(2023年度)年次大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Desiccation tolerance of Bacillus cereus: Contribution of glass transition phenomena of the bacterial cells2023

    • Author(s)
      Tatsuya INOMATA, Kiyoshi KAWAI, Kento KOYAMA, Shige KOSEKI
    • Organizer
      37th EFFoST International Conference 2023
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effect of the glass transition of dried bacterial cells on the survival under desiccation environment2022

    • Author(s)
      Kyeongmin Lee, Saki Tanaka, Tatsuya Inomata, Kiyoshi Kawai, Kento Koyama, Shige Koseki
    • Organizer
      FOODMICRO 2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 吸湿による乾燥微生物の再活性メカニズム2022

    • Author(s)
      川井 清司, 曽我部 知史、中川 洋、山田 武、小関 成樹
    • Organizer
      2022年度量子ビームサイエンスフェスタ 第14回 MLFシンポジウム, 第40回PFシンポジウム
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 中性子非干渉性散乱による生体系および食品中の水のダイナミクスの研究2022

    • Author(s)
      中川 洋
    • Organizer
      水素科学技術連携研究会物理学分科会
    • Related Report
      2022 Annual Research Report
    • Invited

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Published: 2021-04-28   Modified: 2025-01-30  

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