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Development of coherent Raman scattering microendoscope and application to unstained in-situ diagnosis

Research Project

Project/Area Number 21H04950
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Review Section Medium-sized Section 90:Biomedical engineering and related fields
Research InstitutionHokkaido University

Principal Investigator

Hashimoto Mamoru  北海道大学, 情報科学研究院, 教授 (70237949)

Co-Investigator(Kenkyū-buntansha) 七戸 俊明  北海道大学, 医学研究院, 准教授 (70374353)
新岡 宏彦  大阪大学, データビリティフロンティア機構, 特任准教授(常勤) (70552074)
Project Period (FY) 2021-04-05 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥42,640,000 (Direct Cost: ¥32,800,000、Indirect Cost: ¥9,840,000)
Fiscal Year 2023: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2022: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2021: ¥21,190,000 (Direct Cost: ¥16,300,000、Indirect Cost: ¥4,890,000)
Keywordsラマンイメージング / 内視鏡 / 非線形光学 / 非線形ラマン散乱 / 無標識イメージング / 顕微内視鏡
Outline of Research at the Start

色素の静脈投与なしに癌を内視鏡検査で確定診断するために,上部消化管内視鏡(胃カメラ)の 鉗子孔に挿入可能な非線形ラマン散乱プローブ顕微内視鏡を開発する.非線形ラマン散乱を用いることで,無染色・高空間分解能・高速な細胞,細胞核の形状観測を可能とし,内視鏡検査時の 確定診断を実現する.プローブヘッドを受動光学素子のみで構成することで直径3 mm 長さ 20 mm 以下に小型化して,曲がった胃カメラの鉗子孔にも挿入可能とする.さらに,光ファイバーを用いた光源を開発し,持ち運び可能な非線形ラマン散乱プローブ顕微内視鏡を実現する.

Outline of Final Research Achievements

An endoscopic optical system using coherent anti-Stokes Raman scattering (CARS) was investigated to realise an endoscope that enables in situ diagnosis without staining. We proposed a method to avoid light emission from the optical fiber itself, which is a problem when transmitting and imaging excitation light using an optical fiber bundle. We showed that CARS observation is possible by reducing the emission from the optical fiber to 1/3289. We also investigated methods for scanning and controlling the excitation beam's polarisation to improve CARS light emission efficiency. Furthermore, we have studied an image reconstruction method using deep learning to remove the structure of the optical fiber bundle to obtain higher-resolution images.

Academic Significance and Societal Importance of the Research Achievements

消化管内視鏡での検査では,その場で確定診断することは難しく,生検により組織の一部を取り出して確定診断を行う.もし生検を行わずに,しかも安全のために無染色にその場で確定診断を行うことができれば,安全性の向上,患者の負担の軽減,医療コストの削減を達成することが可能となる.このような顕微内視鏡を実現するための光学系システムの検討を行い,イメージングが可能であることを示し,さらにより大きな信号を取得する手法,高精細な画像を取得する方法の開発を行なった.

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Comments on the Screening Results   Annual Research Report
  • Research Products

    (26 results)

All 2023 2022 2021

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 1 results) Presentation (21 results) (of which Int'l Joint Research: 8 results,  Invited: 6 results)

  • [Journal Article] A CMOS Double-Demodulation Lock-In Amplifier for Stimulated Raman Scattering Signal Detection2022

    • Author(s)
      Korakkottil Kunhi Mohd Shukri Bin, L-X. Lioe, K. Yasutomi, K. Kagawa, M. Hashimoto, S. Kawahito
    • Journal Title

      Electronics

      Volume: 12 Issue: 1 Pages: 4-4

    • DOI

      10.3390/electronics12010004

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 深層学習を用いた非線形ラマン散乱硬性内視鏡 による神経イメージングの高速化と神経抽出2022

    • Author(s)
      大和尚記,新岡宏彦,三宅淳,橋本守
    • Journal Title

      光学

      Volume: 41 Pages: 231-237

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Near real-time nerve visualization using coherent Raman scattering rigid endoscope and deep learning-based image processing for nerve-sparing surgery2022

    • Author(s)
      Yamato Naoki、Niioka Hirohiko、Miyake Jun、Hashimoto Mamoru
    • Journal Title

      Proceedings of SPIE

      Volume: 11957 Pages: 46-46

    • DOI

      10.1117/12.2609483

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ultrahigh-speed multiplex coherent anti-Stokes Raman scattering microspectroscopy using scanning elliptical focal spot2021

    • Author(s)
      Kizawa Shun、Hashimoto Mamoru
    • Journal Title

      The Journal of Chemical Physics

      Volume: 155 Issue: 14 Pages: 144201-144201

    • DOI

      10.1063/5.0063987

    • NAID

      120007164559

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Avoidance of four-wave mixing in optical fiber bundle for coherent anti-Stokes Raman scattering endomicroscopy2021

    • Author(s)
      Ogawa Hiroki、Hashimoto Mamoru
    • Journal Title

      Optics Letters

      Volume: 46 Issue: 14 Pages: 3356-3356

    • DOI

      10.1364/ol.425644

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Presentation] Label-free imaging by coherent Raman scattering endoscopy2023

    • Author(s)
      Mamoru Hashimoto
    • Organizer
      2023 International Workshop on the New Frontiers in Convergence Science and Technology of Hokkaido University - Seoul National Universty 26th Joint Symposium, Hokkaido University
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Lipid metabolism imaging by elliptical spots scanning multiplex coherent anti-stokes raman scattering spectromicroscopy2023

    • Author(s)
      Mamoru Hashimoto
    • Organizer
      8th Asian Spectroscopy Conference 2023
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 光ファイバーバンドル非線形ラマン散乱顕微内視鏡における励起光の個別走査による信号増強2023

    • Author(s)
      川崎優花, 大和尚記, 橋本守
    • Organizer
      Optics & Photonics Japan 2023 (OPJ2023)
    • Related Report
      2023 Annual Research Report
  • [Presentation] 光ファイバーバンドルを用いたコヒーレントラマン散乱顕微内視鏡のための偏光補償法の検討2023

    • Author(s)
      五十崎凜太郎, 本間宗一郎, 大和尚記, 橋本守
    • Organizer
      Optics & Photonics Japan 2023 (OPJ2023)
    • Related Report
      2023 Annual Research Report
  • [Presentation] Imaging of trans lipid-mobilization by coherent Raman microspectroscopy2023

    • Author(s)
      Soichiro Homma and Naoki Yamato and Mamoru Hashimoto
    • Organizer
      OSJ-JSAP Joint Symposia on Optics, Optics & Photonics Japan 2023
    • Related Report
      2023 Annual Research Report
  • [Presentation] 無染色に細胞核を可視化する顕微内視鏡実現のための偏光補償法の検討2023

    • Author(s)
      五十崎凜太郎, 川崎優花, 大和尚記, 橋本守
    • Organizer
      第62回日本生体医工学会北海道支部大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Multiplex coherent anti-Stokes Raman 散乱分光顕微鏡を用いた脂肪様細胞のトランス脂肪動員観察2023

    • Author(s)
      本間 宗一郎,大和 尚記,橋本 守
    • Organizer
      第84回応用物理学会秋季学術講演会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 二台の電気光学偏光素子を用いた偏光補償法の検討2023

    • Author(s)
      五十﨑 凜太郎,川﨑 優花,大和 尚紀,橋本 守
    • Organizer
      第58回応用物理学会北海道支部/第19回日本光学会北海道支部合同学術講演会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 楕円スポット走査マルチプレックスCARS分光イメージング2022

    • Author(s)
      橋本守,本間宗一郎 ,木澤駿
    • Organizer
      医用分光学研究会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Fast peripheral nerve imaging with coherent Raman scattering rigid endoscope by noise reduction utilizing deep learning2022

    • Author(s)
      N. Yamato, H. Niioka, J. Miyake, and M. Hashimoto
    • Organizer
      25th Congress of the International Commission for Optics
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Multiplex coherent anti-Stokes Raman scattering microscpectroscopy using elliptical focus spot2022

    • Author(s)
      M. Hashimoto, R. Abe, and S. Kizawa
    • Organizer
      25th Congress of the International Commission for Optics
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Label free isomeric metabolism measurement with multiplex coherent anti-stokes raman scattering microspectroscopy2022

    • Author(s)
      S. Homma and M. Hashimoto,
    • Organizer
      Conference on Lasers and Electro-Optics PacificRim2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Second-harmonic generation arthroscope with integrated femtosecond Yb fiber laser2022

    • Author(s)
      Y. Kashimura, R. Matsuda, N. Yamato, and M. Hashimoto
    • Organizer
      Conference on Lasers and Electro-Optics PacificRim2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Design of dual-wavelength waveplate made of single crystal for coherent anti-Stokes Raman endoscopy2022

    • Author(s)
      Y. Kawasaki and M. Hashimoto
    • Organizer
      Conference on Lasers and Electro-Optics PacificRim2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 非線形ラマン散乱硬性内視鏡と深層学習による神経イメージング装置の開発2022

    • Author(s)
      大和尚記,新岡宏彦,三宅淳,橋本守
    • Organizer
      レーザー学会学術講演会第42回年次大会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Yb ファイバーレーザーを用いた光源一体型第二高調波発生関節鏡の開発2022

    • Author(s)
      松田陸, 橋本守
    • Organizer
      第57回応用物理学会北海道支部/第 18 回日本光学会北海道支部合同学術講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Coherent Raman endoscopy for label-free imaging during surgery2021

    • Author(s)
      M. Hashimoto
    • Organizer
      The 22nd RIES-Hokudai International Symposium
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 非線形ラマン散乱を用いた顕微内視鏡の開発2021

    • Author(s)
      橋本守
    • Organizer
      大学医工連携オンラインセミナー ~光×超音波×近赤外蛍光による医工連携イメージングと健康長寿への道~
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] コヒーレント反ストークスラマン散乱分光顕微鏡の楕円スポットによる信号増強効果の実験的検証2021

    • Author(s)
      本間宗一郎,橋本守
    • Organizer
      レーザ顕微鏡研究会第46回講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Ybファイバーレーザーを用いた第二高調波発生関節鏡の開発2021

    • Author(s)
      松田陸, 橋本守
    • Organizer
      Optics & Photonics Japan 2021
    • Related Report
      2021 Annual Research Report
  • [Presentation] 非線形ラマン散乱硬性内視鏡と深層学習による実時間神経抽出2021

    • Author(s)
      大和尚記,松谷真奈,新岡宏彦,三宅淳,橋本守
    • Organizer
      第60回日本生体医工学会大会・第36回日本生体磁気学会大会
    • Related Report
      2021 Annual Research Report

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Published: 2021-04-28   Modified: 2025-01-30  

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