Development of male infertile model mice by genome editing and comprehensive study of fertilization

• Project/Area Number: 21H05033
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Review Section: Broad Section F
• Research Institution: The University of Osaka
• Principal Investigator: 伊川 正人 大阪大学, 微生物病研究所, 教授 (20304066)
• Co-Investigator(Kenkyū-buntansha): 新岡 宏彦 九州大学, データ駆動イノベーション推進本部, 教授 (70552074) ; 小沢 学 東京大学, 医科学研究所, 准教授 (80608787)
• Project Period (FY): 2021-07-05 – 2026-03-31
• Project Status: Granted (Fiscal Year 2025)
• Budget Amount: ¥188,500,000 (Direct Cost: ¥145,000,000、Indirect Cost: ¥43,500,000); Fiscal Year 2025: ¥35,620,000 (Direct Cost: ¥27,400,000、Indirect Cost: ¥8,220,000); Fiscal Year 2024: ¥38,740,000 (Direct Cost: ¥29,800,000、Indirect Cost: ¥8,940,000); Fiscal Year 2023: ¥38,350,000 (Direct Cost: ¥29,500,000、Indirect Cost: ¥8,850,000); Fiscal Year 2022: ¥37,830,000 (Direct Cost: ¥29,100,000、Indirect Cost: ¥8,730,000); Fiscal Year 2021: ¥37,960,000 (Direct Cost: ¥29,200,000、Indirect Cost: ¥8,760,000)
• Keywords: 実験動物 / ゲノム編集 / 不妊 / 精子 / 受精

Outline of Research at the Start

ポストゲノムプロジェクト時代の生命科学研究には、ゲノムに秘められた無数の遺伝情報を生理機能と結びつける、個体レベルで遺伝子機能を解析する手法や、遺伝子組換え動物そのものが必要不可欠である。本研究では、CRISPR/Cas9ゲノム編集に、ウイルスベクター、ES細胞キメラ解析、生殖工学などを組み合わせることで、ゲノム編集マウス作製・解析プラットフォームを完成させる。さらにその応用として、精巣特異的に発現する約400遺伝子をノックアウトし、雄性不妊モデルマウスの開発・解析を通して哺乳類の精子・受精のバイオロジーを究める。

Outline of Annual Research Achievements

本研究課題では、ゲノム編集マウス作製・解析プラットフォームを完成させて応用し、哺乳類の精子・受精のバイオロジーを究める研究を同時に進めている。
1)ゲノム編集マウス作製・解析プラットフォーム：23年度も、Lipid NanoParticle（LNP）を用いたmRNA導入によるゲノム編集技術開発に取り組んだ。LNPは透明帯を除去することなく受精卵にmRNAを導入できることを利用して、Floxマウスの受精卵にCre mRNAを導入し、ほぼ100％の割合でFlox領域を抜き取ることに成功した。ウイルスベクターや顕微操作を必要としない簡便な受精卵mRNA注入法を確立した。またCas9を全身で発現する遺伝子改変マウスを用いてゲノム編集マウス作製を試みたが、Cas9発現量が少ないためか、実用化には至らなかった。一方で、アデノ随伴ウイルスベクター（AAV）を用いた遺伝子改変マウス作製に取り組み、特定のセロタイプを有するAAVが受精卵への遺伝子導入に適していることを見出した。
2)哺乳類の生殖生物学：精巣特異的に発現する104遺伝子について、KOマウスを作製して交配試験を実施した。その結果、2系統で胚性致死を示し、残る102系統のうち、60系統では妊孕性に問題がなく、8系統で妊孕性が低下、34系統で雄性不妊が認められた。雄性不妊系統については、さらに解析を進め、①CCDC183がないと精子鞭毛形成の際の細胞質陥入が上手くいかないこと、②TEX46がないと精子頭部の形態異常を起こし透明帯を通過できずに雄性不妊となること、③NELL2と共同して精巣上体の分化誘導に働くNICOの同定、などを論文報告した。また国際共同研究により、ZP2の切断による透明帯硬化メカニズムや精子CaチャネルであるCATSPER集合体形成メカニズムに関する論文を報告した。

Current Status of Research Progress

1: Research has progressed more than it was originally planned.

Reason

2023年度は当初予定を上回る104遺伝子のKOマウスを作製し、表現型解析を実施することができた。代表者が責任著者としてだけでなく、高インパクトを有する国際共著論文も多数報告できており、当初の計画以上と判断した。

Strategy for Future Research Activity

研究は順調に進展しており、当初予定通り推進することができると考えている。KOマウス作製による生殖関連遺伝子の機能解析は、競争が激しくなってきているが、国際連携を高めることで、競争力を維持したい。

Assessment Rating

Interim Assessment Comments (Rating)

A: In light of the aim of introducing the research area into the research categories, the expected progress has been made in research.

Research Products

• [Int'l Joint Research] Karolinska Institutet(スウェーデン)
• [Int'l Joint Research] Baylor College of Medicine/Yale University(米国)
• [Int'l Joint Research] Weitzman Institute(イスラエル)
• [Int'l Joint Research] Baylor College of Medicine/Stanford University/Yale University(米国)
• [Int'l Joint Research] Institute of Molecular Pathology(オーストリア)
• [Int'l Joint Research] Utrecht university(オランダ)
• [Int'l Joint Research] Karolinska Institutet(スウェーデン)
• [Int'l Joint Research] Baylor College of Medecine/University of Hawai(米国)
• [Int'l Joint Research] Seoul National University(韓国)
• [Int'l Joint Research] Fudan University(中国)
• [Journal Article] ZP2 cleavage blocks polyspermy by modulating the architecture of the egg coat (2024)
  • Author(s): Nishio S、Emori C、Wiseman B、Fahrenkamp D、Dioguardi E、Zamora-Caballero S、Bokhove M、Han L、Stsiapanava A、Algarra B、Lu Y、Kodani M、Bainbridge RE、Komondor KM、CA、Landreh M、de Sanctis D、Yasumasu S、Ikawa M、Jovine L
  • Journal Title: Cell Volume: 187 Issue: 6 Pages: 1440-1459
  • DOI: 10.1016/j.cell.2024.02.013
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Affinity-tagged SMAD1 and SMAD5 mouse lines reveal transcriptional reprogramming mechanisms during early pregnancy (2024)
  • Author(s): Liao Zian、Tang Suni、Nozawa Kaori、Shimada Keisuke、Ikawa Masahito、Monsivais Diana、Matzuk Martin
  • Journal Title: eLife Volume: 12
  • DOI: 10.7554/elife.91434
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Tex46 knockout male mice are sterile secondary to sperm head malformations and failure to penetrate through the zona pellucida (2024)
  • Author(s): Fujihara Y、Miyata H、Abbasi F、Larasati T、Nozawa K、Yu Z、Ikawa M、Matzuk MM.
  • Journal Title: PNAS Nexus Volume: 3 Issue: 3 Pages: 108-108
  • DOI: 10.1093/pnasnexus/pgae108
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Disruption of testis-enriched cytochrome c oxidase subunit COX6B2 but not COX8C leads to subfertility (2024)
  • Author(s): Shimada Keisuke、Lu Yonggang、Ikawa Masahito
  • Journal Title: Experimental Animals Volume: 73 Issue: 1 Pages: 1-10
  • DOI: 10.1538/expanim.23-0055
  • ISSN: 0007-5124, 1341-1357, 1881-7122
  • Peer Reviewed / Open Access
• [Journal Article] PABPN1L is required for maternal mRNA degradation after meiosis resumption (2024)
  • Author(s): EMORI Chihiro、KODANI Mayo、ABBASI Ferheen、MORI Masashi、IKAWA Masahito
  • Journal Title: Journal of Reproduction and Development Volume: 70 Issue: 1 Pages: 10-17
  • DOI: 10.1262/jrd.2023-077
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed / Open Access
• [Journal Article] Multiple ageing effects on testicular/epididymal germ cells lead to decreased male fertility in mice (2024)
  • Author(s): Tsutomu Endo, Kiyonori Kobayashi, Takafumi Matsumura, Chihiro Emori, Manabu Ozawa, Shimpei Kawamoto, Daisuke Okuzaki, Keisuke Shimada, Haruhiko Miyata, Kentaro Shimada, Mayo Kodani, Yu Ishikawa-Yamauchi, Daisuke Motooka, Eiji Hara, Masahito Ikawa
  • Journal Title: Communications Biology Volume: 7(1) Issue: 1 Pages: 1-6
  • DOI: 10.1038/s42003-023-05685-2
  • Peer Reviewed / Open Access
• [Journal Article] CRISPR/Cas9‐mediated genome editing reveals seven testis‐enriched transmembrane glycoproteins dispensable for male fertility in mice (2023)
  • Author(s): Ogawa Yo、Lu Yonggang、Kiyozumi Daiji、Chang Hsin‐Yi、Ikawa Masahito
  • Journal Title: Andrology Volume: -
  • DOI: 10.1111/andr.13564
  • Peer Reviewed / Open Access
• [Journal Article] Age-related decline in spermatogenic activity accompanied with endothelial cell senescence in male mice_ (2023)
  • Author(s): Manabu Ozawa, Hideto Mori, Tsutomu Endo, Yu Ishikawa-Yamauchi, Daisuke Motooka, Chihiro Emori, Masahiro Ikawa
  • Journal Title: iScience_ Volume: 26(12) Issue: 12 Pages: 108456-108456
  • DOI: 10.1016/j.isci.2023.108456
  • Peer Reviewed / Open Access
• [Journal Article] Adhesion G protein-coupled receptor G2 is dispensable for lumicrine signaling regulating epididymal initial segment differentiation and gene expression (2023)
  • Author(s): Kiyozumi Daiji、Ikawa Masahito
  • Journal Title: Biology of Reproduction Volume: 109 Issue: 4 Pages: 474-481
  • DOI: 10.1093/biolre/ioad087
  • Peer Reviewed / Open Access
• [Journal Article] A CUG-initiated CATSPERθ functions in the CatSper channel assembly and serves as a checkpoint for flagellar trafficking (2023)
  • Author(s): Huang Xiaofang、Miyata Haruhiko、Wang Huafeng、Mori Giulia、Iida-Norita Rie、Ikawa Masahito、Percudani Riccardo、Chung Jean-Ju
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 120 Issue: 39
  • DOI: 10.1073/pnas.2304409120
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] CUB domains are not required for OVCH2 function in sperm maturation in the mouse epididymis (2023)
  • Author(s): Kent Katarzyna、Nozawa Kaori、Sutton Courtney、Daniel Frey、Ikawa Masahito、Garcia Thomas X.、Matzuk Martin M.
  • Journal Title: Andrology Volume: 12 Issue: 3 Pages: 682-697
  • DOI: 10.1111/andr.13508
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Oocyte-specific Wee1-like protein kinase 2 is dispensable for fertility in mice (2023)
  • Author(s): Nozawa Kaori、Liao Zian、Satouh Yuhkoh、Geng Ting、Ikawa Masahito、Monsivais Diana、Matzuk Martin M.
  • Journal Title: PLOS ONE Volume: 18 Issue: 8 Pages: e0289083-e0289083
  • DOI: 10.1371/journal.pone.0289083
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular dissection and testing of PRSS37 function through LC?MS/MS and the generation of a PRSS37 humanized mouse model (2023)
  • Author(s): Sutton Courtney、Nozawa Kaori、Kent Katarzyna、Saltzman Alexander、Leng Mei、Nagarajan Sureshbabu、Malovannaya Anna、Ikawa Masahito、Garcia Thomas X.、Matzuk Martin M.
  • Journal Title: Scientific Reports Volume: 13 Issue: 1 Pages: 11374-11374
  • DOI: 10.1038/s41598-023-37700-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Involvement of linker histone variant H1a in the regulation of early preimplantation development in mice (2023)
  • Author(s): FUNAYA Satoshi、WANG Yuan、SUZUKI Masataka G.、IKAWA Masahito、AOKI Fugaku
  • Journal Title: Journal of Reproduction and Development Volume: 69 Issue: 3 Pages: 178-182
  • DOI: 10.1262/jrd.2023-013
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed / Open Access
• [Journal Article] A small secreted protein NICOL regulates lumicrine-mediated sperm maturation and male fertility (2023)
  • Author(s): Kiyozumi Daiji、Shimada Kentaro、Chalick Michael、Emori Chihiro、Kodani Mayo、Oura Seiya、Noda Taichi、Endo Tsutomu、Matzuk Martin M.、Wreschner Daniel H.、Ikawa Masahito
  • Journal Title: Nature Communications Volume: 14 Issue: 1 Pages: 2354-2354
  • DOI: 10.1038/s41467-023-37984-x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] TSKS localizes to nuage in spermatids and regulates cytoplasmic elimination during spermiation (2023)
  • Author(s): Shimada Keisuke、Park Soojin、Oura Seiya、Noda Taichi、Morohoshi Akane、Matzuk Martin M.、Ikawa Masahito
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 120 Issue: 11 Pages: 1-10
  • DOI: 10.1073/pnas.2221762120
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 1700029I15Rik orchestrates the biosynthesis of acrosomal membrane proteins required for sperm-egg interaction (2023)
  • Author(s): Lu Yonggang、Shimada Kentaro、Tang Shaogeng、Zhang Jingjing、Ogawa Yo、Noda Taichi、Shibuya Hiroki、Ikawa Masahito
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 120 Issue: 8 Pages: 1-11
  • DOI: 10.1073/pnas.2207263120
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] ADAD2 functions in spermiogenesis and piRNA biogenesis in mice (2023)
  • Author(s): Lu Yonggang、Nagamori Ippei、Kobayashi Hisato、Kojima‐Kita Kanako、Shirane Kenjiro、Chang Hsin‐Yi、Nishimura Toru、Koyano Takayuki、Yu Zhifeng、Castaneda Julio M.、Matsuyama Makoto、Kuramochi‐Miyagawa Satomi、Matzuk Martin M.、Ikawa Masahito
  • Journal Title: Andrology Volume: 11 Issue: 4 Pages: 698-709
  • DOI: 10.1111/andr.13400
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Testis-enriched ferlin, FER1L5, is required for Ca ²⁺ -activated acrosome reaction and male fertility (2023)
  • Author(s): Morohoshi Akane、Miyata Haruhiko、Tokuhiro Keizo、Iida-Norita Rie、Noda Taichi、Fujihara Yoshitaka、Ikawa Masahito
  • Journal Title: Science Advances Volume: 9 Issue: 4
  • DOI: 10.1126/sciadv.ade7607
  • Peer Reviewed / Open Access
• [Journal Article] Testis‐specific proteins, TSNAXIP1 and 1700010I14RIK, are important for sperm motility and male fertility in mice (2023)
  • Author(s): Kaneda Yuki、Miyata Haruhiko、Shimada Keisuke、Oura Seiya、Ikawa Masahito
  • Journal Title: Andrology Volume: - Issue: 5 Pages: 1-9
  • DOI: 10.1111/andr.13378
  • Peer Reviewed / Open Access
• [Journal Article] Efficient simultaneous double DNA knock-in in murine embryonic stem cells by CRISPR/Cas9 ribonucleoprotein-mediated circular plasmid targeting for generating gene-manipulated mice (2022)
  • Author(s): Ozawa Manabu、Taguchi Jumpei、Katsuma Kento、Ishikawa-Yamauchi Yu、Kikuchi Mio、Sakamoto Reiko、Yamada Yasuhiro、Ikawa Masahito
  • Journal Title: Scientific Reports Volume: 12 Issue: 1 Pages: 21558-21558
  • DOI: 10.1038/s41598-022-26107-z
  • Peer Reviewed / Open Access
• [Journal Article] PDCL2 is essential for sperm acrosome formation and male fertility in mice (2022)
  • Author(s): Fujihara Y、Kobayashi K、Abbasi F、Endo T、Yu Z、Ikawa M、Matzuk MM.
  • Journal Title: Andrology Volume: - Issue: 5 Pages: 789-798
  • DOI: 10.1111/andr.13329
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Generation of humanized LDHC knock‐in mice as a tool to assess human LDHC‐targeting contraceptive drugs (2022)
  • Author(s): Iida‐Norita Rie、Miyata Haruhiko、Kaneda Yuki、Emori Chihiro、Noda Taichi、Nakagawa Tatsuya、Matzuk Martin M.、Ikawa Masahito
  • Journal Title: Andrology Volume: - Issue: 5 Pages: 1-9
  • DOI: 10.1111/andr.13359
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] CRISPR/Cas9‐mediated disruption of <i>lipocalins</i> , <i>Ly6g5b</i> , and <i>Ly6g5c</i> causes male subfertility in mice (2022)
  • Author(s): Sakurai N、Fujihara Y、Kobayashi K、Ikawa M.
  • Journal Title: Andrology Volume: - Issue: 5 Pages: 981-990
  • DOI: 10.1111/andr.13350
  • Peer Reviewed / Open Access
• [Journal Article] Eukaryotic fertilization and gamete fusion at a glance (2022)
  • Author(s): Lu Yonggang、Ikawa Masahito
  • Journal Title: Journal of Cell Science Volume: 135 Issue: 22
  • DOI: 10.1242/jcs.260296
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Human sperm TMEM95 binds eggs and facilitates membrane fusion (2022)
  • Author(s): Tang Shaogeng、Lu Yonggang、Skinner Will M.、Sanyal Mrinmoy、Lishko Polina V.、Ikawa Masahito、Kim Peter S.
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 119 Issue: 40
  • DOI: 10.1073/pnas.2207805119
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models (2022)
  • Author(s): Ozawa Manabu、Emori Chihiro、Ikawa Masahito
  • Journal Title: Journal of Visualized Experiments Volume: 186 Issue: 186 Pages: 1-13
  • DOI: 10.3791/64385
  • Peer Reviewed / Open Access
• [Journal Article] Proteolysis in Reproduction: Lessons From Gene-Modified Organism Studies (2022)
  • Author(s): Kiyozumi Daiji、Ikawa Masahito
  • Journal Title: Frontiers in Endocrinology Volume: 13 Pages: 876370-876370
  • DOI: 10.3389/fendo.2022.876370
  • Peer Reviewed / Open Access
• [Journal Article] IRGC1, a testis-enriched immunity related GTPase, is important for fibrous sheath integrity and sperm motility in mice (2022)
  • Author(s): Kaneda Yuki、Miyata Haruhiko、Shimada Keisuke、Oyama Yuki、Iida-Norita Rie、Ikawa Masahito
  • Journal Title: Developmental Biology Volume: 488 Pages: 104-113
  • DOI: 10.1016/j.ydbio.2022.05.011
  • Peer Reviewed / Open Access
• [Journal Article] TULP2 deletion mice exhibit abnormal outer dense fiber structure and male infertility (2022)
  • Author(s): Oyama Yuki、Miyata Haruhiko、Shimada Keisuke、Larasati Tamara、Fujihara Yoshitaka、Ikawa Masahito
  • Journal Title: Reproductive Medicine and Biology Volume: 21 Issue: 1 Pages: 1-8
  • DOI: 10.1002/rmb2.12467
  • Peer Reviewed / Open Access
• [Journal Article] Sperm membrane proteins DCST1 and DCST2 are required for sperm-egg interaction in mice and fish (2022)
  • Author(s): Noda Taichi、Blaha Andreas、Fujihara Yoshitaka、Gert Krista R.、Emori Chihiro、Deneke Victoria E.、Oura Seiya、Panser Karin、Lu Yonggang、Berent Sara、Kodani Mayo、Cabrera-Quio Luis Enrique、Pauli Andrea、Ikawa Masahito
  • Journal Title: Communications Biology Volume: 5 Issue: 1 Pages: 1-11
  • DOI: 10.1038/s42003-022-03289-w
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Sperm IZUMO1 Is Required for Binding Preceding Fusion With Oolemma in Mice and Rats (2022)
  • Author(s): Matsumura Takafumi、Noda Taichi、Satouh Yuhkoh、Morohoshi Akane、Yuri Shunsuke、Ogawa Masaki、Lu Yonggang、Isotani Ayako、Ikawa Masahito
  • Journal Title: Frontiers in Cell and Developmental Biology Volume: 9 Pages: 1-10
  • DOI: 10.3389/fcell.2021.810118
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] C2cd6-encoded CatSperτ targets sperm calcium channel to Ca2+ signaling domains in the flagellar membrane (2022)
  • Author(s): Hwang Jae Yeon、Wang Huafeng、Lu Yonggang、Ikawa Masahito、Chung Jean-Ju
  • Journal Title: Cell Reports Volume: 38 Issue: 3 Pages: 110226-110226
  • DOI: 10.1016/j.celrep.2021.110226
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The motor domain of testis-enriched kinesin KIF9 is essential for its localization in the mouse flagellum (2022)
  • Author(s): Miyata Haruhiko、Oyama Yuki、Kaneda Yuki、Ikawa Masahito
  • Journal Title: Experimental Animals Volume: 71 Issue: 1 Pages: 46-52
  • DOI: 10.1538/expanim.21-0082
  • NAID: 130008158678
  • ISSN: 0007-5124, 1341-1357, 1881-7122
  • Peer Reviewed / Open Access
• [Journal Article] CRISPR/Cas9-mediated genome editing reveals 12 testis-enriched genes dispensable for male fertility in mice (2022)
  • Author(s): Oyama Yuki、Miyata Haruhiko、Shimada Keisuke、Fujihara Yoshitaka、Tokuhiro Keizo、Garcia ThomasX、Matzuk MartinM、Ikawa Masahito
  • Journal Title: Asian Journal of Andrology Volume: in press Issue: 3 Pages: 0-0
  • DOI: 10.4103/aja.aja_63_21
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] FAM71F1 binds to RAB2A and RAB2B and is essential for acrosome formation and male fertility in mice (2021)
  • Author(s): Morohoshi Akane、Miyata Haruhiko、Oyama Yuki、Oura Seiya、Noda Taichi、Ikawa Masahito
  • Journal Title: Development Volume: 148 Issue: 21
  • DOI: 10.1242/dev.199644
  • Peer Reviewed / Open Access
• [Journal Article] The conserved fertility factor SPACA4/Bouncer has divergent modes of action in vertebrate fertilization (2021)
  • Author(s): Fujihara Yoshitaka、Herberg Sarah、Blaha Andreas、Panser Karin、Kobayashi Kiyonori、Larasati Tamara、Novatchkova Maria、Theussl Hans-Christian、Olszanska Olga、Ikawa Masahito、Pauli Andrea
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 118 Issue: 39
  • DOI: 10.1073/pnas.2108777118
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice (2021)
  • Author(s): Miyata Haruhiko、Oura Seiya、Morohoshi Akane、Shimada Keisuke、Mashiko Daisuke、Oyama Yuki、Kaneda Yuki、Matsumura Takafumi、Abbasi Ferheen、Ikawa Masahito
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 118 Issue: 35 Pages: 1-9
  • DOI: 10.1073/pnas.2106673118
  • Peer Reviewed / Open Access
• [Presentation] Testis-enriched genes and their functions during spermatogenesis and sperm function (2023)
  • Author(s): Masahito Ikawa
  • Organizer: Spermatogenesis Conference (Fusion)1st Spermatogenesis Conference
  • Int'l Joint Research / Invited
• [Presentation] ゲノム編集マウスを通してみる精子成熟と受精 (2023)
  • Author(s): 伊川正人
  • Organizer: 第116回日本繁殖生物学会大会
  • Invited
• [Presentation] CRISPR/Cas9 mediated-KO screening identified essential genes for fertilization and oocyte activation (2023)
  • Author(s): Masahito Ikawa
  • Organizer: ASPIRE (Asia Pacific Initiative on Reproduction)
  • Int'l Joint Research / Invited
• [Presentation] Gene Manipulated Animals and the Study of Reproduction (2023)
  • Author(s): Masahito Ikawa
  • Organizer: 第2回非ゲノム情報複製国際シンポジウム
  • Invited
• [Presentation] ゲノム編集マウスを通して見る受精 (2023)
  • Author(s): 伊川正人
  • Organizer: 第64回日本卵子学会
  • Invited
• [Presentation] Testis-enriched genes and their function (2023)
  • Author(s): Masahito Ikawa
  • Organizer: 30 years of (Re) Productive Biology - A Symposium Honoring Professor Martin M. MATZUK at BCM(Baylor College of Medicine)
  • Int'l Joint Research / Invited
• [Presentation] Testis-enriched genes and their functions for spermatogenesis and fertilization (2023)
  • Author(s): Masahito Ikawa
  • Organizer: The first Asian Congress for Reproductive Immunology (ACRI 2023)
  • Int'l Joint Research / Invited
• [Presentation] ゲノム編集を通して見る受精メカニズム (2023)
  • Author(s): 伊川正人
  • Organizer: 第28回日本臨床エンブリオロジスト学会
  • Invited
• [Presentation] Lumicrine Factors in the Regulation of Spermatogenesis (2022)
  • Author(s): Masahito Ikawa
  • Organizer: North American Testis workshop 2022
  • Int'l Joint Research / Invited
• [Presentation] Lumicrine Factors in the Regulation of Spermiogenesis (2022)
  • Author(s): Masahito Ikawa
  • Organizer: 第55回日本発生生物学会
  • Invited
• [Presentation] ゲノム編集マウスとアンドロロジー研究 (2022)
  • Author(s): 伊川正人
  • Organizer: 日本アンドロロジー学会 第41回学術大会
  • Invited
• [Presentation] Lumicrine factors in the regulation of spermatogenesis (2022)
  • Author(s): Masahito Ikawa
  • Organizer: Von Behring-Rontgen-Symposium 2022 "The Epididymis"
  • Int'l Joint Research / Invited
• [Presentation] ゲノム編集マウスと精子研究への応用 (2022)
  • Author(s): 伊川正人
  • Organizer: 第10回実験動物科学シンポジウム
  • Invited
• [Presentation] Testis-enriched genes and their functions for spermatogenesis andfertilization (2022)
  • Author(s): Masahito Ikawa
  • Organizer: The International Symposium “Totipotency and Germ Cell Development”
  • Int'l Joint Research / Invited
• [Presentation] CRISPR/Cas9GenomeEdinganditsApplicaonintheStudyofFerlizaon (2021)
  • Author(s): Masahito Ikawa
  • Organizer: ASA 2021 Annual Meeting
  • Int'l Joint Research / Invited
• [Presentation] 遺伝子改変マウス作製技術の開発と生殖生物学研究への応用 (2021)
  • Author(s): 伊川 正人
  • Organizer: 第68回日本実験動物学会総会・受賞講演
  • Invited
• [Presentation] ゲノム編集を用いた遺伝子改変マウス作製～最新の方法論と将来展望 (2021)
  • Author(s): 伊川 正人
  • Organizer: 第57回日本小児循環器学会・学術集会
  • Invited
• [Presentation] Mammalian sperm-oocyte fusion, ever more puzzling (2021)
  • Author(s): Masahito Ikawa
  • Organizer: Gametes Meet in the time of COVID-19
  • Int'l Joint Research / Invited
• [Presentation] 遺伝子改変マウスと生殖生物学研究への応用 (2021)
  • Author(s): 伊川 正人
  • Organizer: 日本畜産学会第129回大会
  • Invited
• [Presentation] トランスジェニック、ノックアウトからゲノム編集へ：遺伝子改変マウスと生殖生物学研究への応用 (2021)
  • Author(s): 伊川 正人
  • Organizer: 理研BRC 20周年記念シンポジウム
  • Invited
• [Presentation] 卵が間違って精子核を捨てないしくみ (2021)
  • Author(s): 伊川 正人
  • Organizer: 新学術・全能性プログラム 非公開シンポジウム
  • Invited
• [Remarks] ラボHP
  • URL: https://egr.biken.osaka-u.ac.jp/
• [Remarks] Google Scholar
  • URL: https://scholar.google.com/citations?user=AM4gdngAAAAJ&hl=en
• [Remarks] 研究室HP
  • URL: https://egr.biken.osaka-u.ac.jp/
• [Remarks] 研究室ホームページ
  • URL: https://egr.biken.osaka-u.ac.jp/
• [Patent(Industrial Property Rights)] 培養装置、培養対象物観察法、及び培養チップ (2022)
  • Inventor(s): 木村、白井、中村、伊川、藤原、小川、山内、永田
  • Industrial Property Rights Holder: 木村、白井、中村、伊川、藤原、小川、山内、永田
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2022-177449
  • Filing Date: 2022
• [Funded Workshop] The International Symposium “Totipotency and Germ Cell Development” (2022)