Plasticity of mineral element transport system in response to soil environmental fluctuations in plants

• Project/Area Number: 21H05034
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Review Section: Broad Section F
• Research Institution: Okayama University
• Principal Investigator: 馬 建鋒 岡山大学, 資源植物科学研究所, 教授 (80260389)
• Co-Investigator(Kenkyū-buntansha): 山地 直樹 岡山大学, 資源植物科学研究所, 准教授 (00444646) ; 宮地 孝明 岡山大学, 自然生命科学研究支援センター, 研究教授 (40550314) ; 三谷 奈見季 岡山大学, 資源植物科学研究所, 准教授 (40581020) ; 菅 倫寛 岡山大学, 異分野基礎科学研究所, 教授 (60634920)
• Project Period (FY): 2021-07-05 – 2026-03-31
• Project Status: Granted (Fiscal Year 2024)
• Budget Amount: ¥189,280,000 (Direct Cost: ¥145,600,000、Indirect Cost: ¥43,680,000); Fiscal Year 2025: ¥34,840,000 (Direct Cost: ¥26,800,000、Indirect Cost: ¥8,040,000); Fiscal Year 2024: ¥35,750,000 (Direct Cost: ¥27,500,000、Indirect Cost: ¥8,250,000); Fiscal Year 2023: ¥36,270,000 (Direct Cost: ¥27,900,000、Indirect Cost: ¥8,370,000); Fiscal Year 2022: ¥37,310,000 (Direct Cost: ¥28,700,000、Indirect Cost: ¥8,610,000); Fiscal Year 2021: ¥45,110,000 (Direct Cost: ¥34,700,000、Indirect Cost: ¥10,410,000)
• Keywords: 環境変動 / ミネラル輸送 / イネ / 可塑性 / トランスポーター / 制御 / 根

Outline of Research at the Start

地球規模の気候変動により、土壌の水分状態が大きく変動するため、作物の生育に欠かせない土壌中の養分濃度や化学形態が激しく変化している。また過度の施肥により、土壌の酸性化が進み、作物の生育を阻害する有害金属が溶出してしまい、作物の生産性や安全性において大きな問題となっている。本研究は主に”水陸両用”のイネを用いて、土壌ミネラル環境変動に可塑的に対応する植物の輸送システムを分子レベルで解明し、将来環境変動に適応できる環境応答型作物の作出に寄与する。

Outline of Annual Research Achievements

SIET4は葉の表面やケイ化機動細胞へとケイ素を排出する役割を持つことを解明した。SIET4はケイ素が蓄積する葉の表皮細胞とケイ化機動細胞の隣の細胞に局在する。SIET4 遺伝子を破壊すると、ケイ素ある条件下で栽培した場合は、生育が著しく抑制され、最後は死に至った。またOsOPT7が、OsOPT7は節や葉鞘において導管から二価鉄イオンをアンローディングする役割を明らかにした。プロテオリポソームを用いた解析により、OsOPT7は二価鉄イオン(Fe2+)をプロトンとの共輸送によって細胞内に取り込む輸送活性がみられた。OsOPT7は節の肥大維管束木部柔細胞や葉鞘の維管束柔細胞などに局在し、鉄欠乏条件下で発現が増加した。遺伝子発現抑制株では、新葉や穂への鉄分配が減少し、また葉鞘組織への鉄の分配も減少した。そのほか、イネ葉鞘と葉身間のマンガン分配に必要な輸送体OsNramp5、種子へのカルシム集積に関与する輸送体K5.2等の機能を解明した。
ミネラル環境応答シグナル因子を同定しるために、レーザー彫刻機とデジタルマイクロスコープを用いてDIYインセクトレーザー装置を開発し、イネの篩管液の採取が可能になった。ウエスタン解析やプロテオーム解析によって、ケイ素欠乏条件のイネ篩管液から、ケイ素吸収を制御する長距離シグナルタンパク質であるSSSを検出した。またCo-IPの手法を用いてこのタンパク質と複合体を形成するパートナーの探索を試みた。その結果、転写活性があり、一過性発現の実験系においてはケイ酸輸送体Lsi1の転写を負に制御する因子を単離した。
クライオ電子顕微鏡を用いてマンガン輸送体の立体構造を高分解能で解析した。マンガン輸送体について、基質の結合していない状態、マンガン基質が結合した状態、カドミウム基質が結合した状態の３つの立体構造を比較して、基質の選択に重要なアミノ酸残基の候補を明らかにした。

Current Status of Research Progress

1: Research has progressed more than it was originally planned.

Reason

予定より多くの輸送体を同定し、いくつかの発見をトップジャーナルに発表することができた。

Strategy for Future Research Activity

共同研究者と緊密に連携して、引き続き新規植物ミネラル輸送体の同定と機能解析、環境応答機構の解明、シグナル因子の同定、結晶構造解析などを行う。

Assessment Rating

Interim Assessment Comments (Rating)

A+: In light of the aim of introducing the research area into the research categories, more progress has been made in research than expected.

Research Products

• [Journal Article] Transport of mineral elements in plants and me (2024)
  • Author(s): 馬 建鋒
  • Journal Title: Fertilizer Science Volume: 45 Issue: 45 Pages: 109-139
  • DOI: 10.57411/fertilizerscience.45.45_109
  • ISSN: 0387-2718, 2758-321X
  • Year and Date: 2024-02-20
  • Peer Reviewed / Open Access
• [Journal Article] Knockout of a rice <i>K5.2</i> gene increases Ca accumulation in the grain (2024)
  • Author(s): Wang Peitong、Yamaji Naoki、Mitani‐Ueno Namiki、Ge Jun、Ma Jian Feng
  • Journal Title: Journal of Integrative Plant Biology Volume: 66 Issue: 2 Pages: 252-264
  • DOI: 10.1111/jipb.13587
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A silicon transporter gene required for healthy growth of rice on land (2023)
  • Author(s): Mitani-Ueno Namiki、Yamaji Naoki、Huang Sheng、Yoshioka Yuma、Miyaji Takaaki、Ma Jian Feng
  • Journal Title: Nature Communications Volume: 14 Issue: 1 Pages: 6522-6522
  • DOI: 10.1038/s41467-023-42180-y
  • Peer Reviewed / Open Access
• [Journal Article] Dissecting the promotional effect of zinc on cadmium translocation from roots to shoots in rice (2023)
  • Author(s): Chang Jia-Dong、Huang Sheng、Wiseno Indi、Sui Fu-Qing、Feng Fan、Zheng Luqing、Ma Jian Feng、Zhao Fang-Jie
  • Journal Title: Journal of Experimental Botany Volume: 74 Issue: 21 Pages: 6790-6803
  • DOI: 10.1093/jxb/erad330
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Local distribution of manganese to leaf sheath is mediated by OsNramp5 in rice (2023)
  • Author(s): Huang Sheng、Konishi Noriyuki、Yamaji Naoki、Ma Jian Feng
  • Journal Title: New Phytologist Volume: 241 Issue: 4 Pages: 1708-1719
  • DOI: 10.1111/nph.19454
  • Peer Reviewed / Open Access
• [Journal Article] Polar localization of a rice silicon transporter requires isoleucine at both C- and N-termini as well as positively charged residues (2023)
  • Author(s): Konishi Noriyuki、Mitani-Ueno Namiki、Yamaji Naoki、Ma Jian Feng
  • Journal Title: The Plant Cell Volume: 35 Issue: 6 Pages: 2232-2250
  • DOI: 10.1093/plcell/koad073
  • Peer Reviewed / Open Access
• [Journal Article] A vacuolar transporter plays important roles in zinc and cadmium accumulation in rice grain (2023)
  • Author(s): Ning Min、Liu Shi Jia、Deng Fenglin、Huang Liyu、Li Hu、Che Jing、Yamaji Naoki、Hu Fengyi、Lei Gui Jie
  • Journal Title: New Phytologist Volume: 239 Issue: 5 Pages: 1919-1934
  • DOI: 10.1111/nph.19070
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cell-Type-Dependent but CME-Independent Polar Localization of Silicon Transporters in Rice (2022)
  • Author(s): Konishi Noriyuki、Huang Sheng、Yamaji Naoki、Ma Jian Feng
  • Journal Title: Plant and Cell Physiology Volume: - Issue: 5 Pages: 699-712
  • DOI: 10.1093/pcp/pcac032
  • Peer Reviewed
• [Journal Article] <scp>NRAMP6</scp> and <scp>NRAMP1</scp> cooperatively regulate root growth and manganese translocation under manganese deficiency in Arabidopsis (2022)
  • Author(s): Li Lun、Zhu Zongzheng、Liao Yonghui、Yang Changhong、Fan Ni、Zhang Jie、Yamaji Naoki、Dirick L?on、Ma Jian Feng、Curie Catherine、Huang Chao‐Feng
  • Journal Title: The Plant Journal Volume: 110 Issue: 6 Pages: 1564-1577
  • DOI: 10.1111/tpj.15754
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Duplication of a manganese/cadmium transporter gene reduces cadmium accumulation in rice grain (2022)
  • Author(s): Yu En、Wang Wenguang、Yamaji Naoki、Fukuoka Shuichi、Che Jing、Ueno Daisei、Ando Tsuyu、Deng Fenglin、Hori Kiyosumi、Yano Masahiro、Shen Ren Fang、Ma Jian Feng
  • Journal Title: Nature Food Volume: 3 Issue: 8 Pages: 597-607
  • DOI: 10.1038/s43016-022-00569-w
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A novel kinase subverts aluminium resistance by boosting ornithine decarboxylase‐dependent putrescine biosynthesis (2022)
  • Author(s): Liu Xiang P.、Gao Li J.、She Ben T.、Li Gui X.、Wu Yun R.、Xu Ji M.、Ding Zhong J.、Ma Jian F.、Zheng Shao J.
  • Journal Title: Plant, Cell &amp; Environment Volume: 45 Issue: 8 Pages: 2520-2532
  • DOI: 10.1111/pce.14371
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A Golgi‐localized glycosyltransferase, <scp>OsGT14;1</scp> , is required for growth of both roots and shoots in rice (2022)
  • Author(s): Wang Peitong、Yamaji Naoki、Ma Jian Feng
  • Journal Title: The Plant Journal Volume: 111 Issue: 4 Pages: 923-935
  • DOI: 10.1111/tpj.15897
  • Peer Reviewed
• [Journal Article] A tonoplast‐localized magnesium transporter is crucial for stomatal opening in Arabidopsis under high Mg ²⁺ conditions (2022)
  • Author(s): Inoue Shin‐ichiro、Hayashi Maki、Huang Sheng、Yokosho Kengo、Gotoh Eiji、Ikematsu Shuka、Okumura Masaki、Suzuki Takamasa、Kamura Takumi、Kinoshita Toshinori、Ma Jian Feng
  • Journal Title: New Phytologist Volume: 236 Issue: 3 Pages: 864-877
  • DOI: 10.1111/nph.18410
  • Peer Reviewed / Open Access
• [Journal Article] FE UPTAKE‐INDUCING PEPTIDE1 maintains Fe translocation by controlling Fe deficiency response genes in the vascular tissue of <i>Arabidopsis</i> (2022)
  • Author(s): Okada Satoshi、Lei Gui J.、Yamaji Naoki、Huang Sheng、Ma Jian F.、Mochida Keiichi、Hirayama Takashi
  • Journal Title: Plant, Cell &amp; Environment Volume: 45 Issue: 11 Pages: 3322-3337
  • DOI: 10.1111/pce.14424
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Linking root morphology and anatomy with transporters for mineral element uptake in plants (2022)
  • Author(s): En Yu、Yamaji Naoki、Ma Jian Feng
  • Journal Title: Plant and Soil Volume: 484 Issue: 1-2 Pages: 1-12
  • DOI: 10.1007/s11104-022-05692-y
  • Peer Reviewed
• [Journal Article] Plant Hormone and Inorganic Ion Concentrations in the Xylem Exudate of Grafted Plants Depend on the Scion?Rootstock Combination (2022)
  • Author(s): Kawaguchi Kohei、Nakaune Makoto、Ma Jian Feng、Kojima Mikiko、Takebayashi Yumiko、Sakakibara Hitoshi、Otagaki Shungo、Matsumoto Shogo、Shiratake Katsuhiro
  • Journal Title: Plants Volume: 11 Issue: 19 Pages: 2594-2594
  • DOI: 10.3390/plants11192594
  • Peer Reviewed
• [Journal Article] The ferroxidases LPR1 and LPR2 control iron translocation in the xylem of Arabidopsis plants (2022)
  • Author(s): Xu Zhong-Rui、Cai Mei-Ling、Yang Ying、You Ting-Ting、Ma Jian Feng、Wang Peng、Zhao Fang-Jie
  • Journal Title: Molecular Plant Volume: 15 Issue: 12 Pages: 1962-1975
  • DOI: 10.1016/j.molp.2022.11.003
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Role of calcium signaling in aluminum tolerance in Arabidopsis (2022)
  • Author(s): Huang Sheng、Ma Jian Feng
  • Journal Title: New Phytologist Volume: 233 Issue: 6 Pages: 2327-2329
  • DOI: 10.1111/nph.17953
  • Peer Reviewed
• [Journal Article] A pericycle‐localized silicon transporter for efficient xylem loading in rice (2022)
  • Author(s): Huang Sheng、Yamaji Naoki、Sakurai Gen、Mitani‐Ueno Namiki、Konishi Noriyuki、Ma Jian Feng
  • Journal Title: New Phytologist Volume: 234 Issue: 1 Pages: 197-208
  • DOI: 10.1111/nph.17959
  • Peer Reviewed
• [Journal Article] A crucial role for a node‐localized transporter, HvSPDT, in?loading phosphorus into barley grains (2022)
  • Author(s): Gu Mian、Huang Hengliang、Hisano Hiroshi、Ding Guangda、Huang Sheng、Mitani‐Ueno Namiki、Yokosho Kengo、Sato Kazuhiro、Yamaji Naoki、Ma Jian Feng
  • Journal Title: New Phytologist Volume: 234 Issue: 4 Pages: 1249-1261
  • DOI: 10.1111/nph.18057
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Lateral roots but not root hairs contribute to high uptake of manganese and cadmium in rice (2021)
  • Author(s): Yu En、Yamaji Naoki、Mao Chuanzao、Wang Hua、Ma Jian Feng
  • Journal Title: Journal of Experimental Botany Volume: - Issue: 20 Pages: 7219-7228
  • DOI: 10.1093/jxb/erab329
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Structural basis for high selectivity of a rice silicon channel Lsi1 (2021)
  • Author(s): Saitoh Yasunori、Mitani-Ueno Namiki、Saito Keisuke、Matsuki Kengo、Huang Sheng、Yang Lingli、Yamaji Naoki、Ishikita Hiroshi、Shen Jian-Ren、Ma Jian Feng、Suga Michihiro
  • Journal Title: Nature Communications Volume: 12 Issue: 1 Pages: 6236-6245
  • DOI: 10.1038/s41467-021-26535-x
  • NAID: 120007170514
  • Peer Reviewed / Open Access
• [Journal Article] Metalloid transporters and their regulation in plants (2021)
  • Author(s): Yamaji Naoki、Ma Jian Feng
  • Journal Title: Plant Physiology Volume: - Issue: 4 Pages: 1929-1939
  • DOI: 10.1093/plphys/kiab326
  • Peer Reviewed
• [Journal Article] Zinc transport in rice: how to balance optimal plant requirements and human nutrition (2021)
  • Author(s): Huang Sheng、Yamaji Naoki、Feng Ma Jian
  • Journal Title: Journal of Experimental Botany Volume: 73 Issue: 6 Pages: 1800-1808
  • DOI: 10.1093/jxb/erab478
  • Peer Reviewed
• [Journal Article] Three polarly localized ammonium transporter 1 members are cooperatively responsible for ammonium uptake in rice under low ammonium condition (2021)
  • Author(s): Konishi Noriyuki、Ma Jian Feng
  • Journal Title: New Phytologist Volume: 232 Issue: 4 Pages: 1778-1792
  • DOI: 10.1111/nph.17679
  • Peer Reviewed
• [Journal Article] Boron uptake in rice is regulated post-translationally via a clathrin-independent pathway (2021)
  • Author(s): Huang Sheng、Konishi Noriyuki、Yamaji Naoki、Shao Ji Feng、Mitani-Ueno Namiki、Ma Jian Feng
  • Journal Title: Plant Physiology Volume: 188 Issue: 3 Pages: 1649-1664
  • DOI: 10.1093/plphys/kiab575
  • Peer Reviewed
• [Presentation] イネマンガン輸送体OsNramp5の偏在と膜輸送に関わる領域の同定 (2024)
  • Author(s): 小西範幸・馬 建鋒
  • Organizer: 第65回日本植物生理学会年会
• [Presentation] DIYインセクトレーザー装置によるイネの篩管液採集法の開発 (2024)
  • Author(s): 山地直樹・新屋友規・三谷奈見季・Ivan Galis・馬 建鋒
  • Organizer: 第65回日本植物生理学会年会
• [Presentation] Exploring mineral transporter genes for better and safe barley production (2023)
  • Author(s): Ma, J. F.
  • Organizer: 3rd Barley Mutant Conference
  • Int'l Joint Research / Invited
• [Presentation] Molecular mechanisms of crops for high tolerance to mineral stresses in acid soils (2023)
  • Author(s): Ma, J. F.
  • Organizer: The 11th International Symposium on Plant-Soil Interactions at Low pH (PSILPH)
  • Int'l Joint Research / Invited
• [Presentation] Posttranslational regulation of ART1 in rice (2023)
  • Author(s): Konishi, N., Ma, J. F.
  • Organizer: The 11th International Symposium on Plant-Soil Interactions at Low pH (PSILPH)
  • Int'l Joint Research
• [Presentation] OsOPT7 mediates xylem unloading of Fe for its preferential distribution in rice. (2023)
  • Author(s): Yamaji, N., Yoshioka, Y., Huang, S., Miyaji, T., Sasaki, A., Ma, J. F.
  • Organizer: The 11th International Symposium on Plant-Soil Interactions at Low pH (PSILPH)
  • Int'l Joint Research
• [Presentation] イネのケイ酸チャネルLsi6の変異体を用いたケイ素吸収・分配制御の詳細解析 (2023)
  • Author(s): 山地直樹・櫻井 玄・三谷奈見季・黄 勝・馬 建鋒
  • Organizer: 日本土壌肥料学会2023年度愛媛大会
• [Presentation] ケイ素応答性長距離シグナルの同定 (2023)
  • Author(s): 三谷奈見季・山地直樹・小西範幸・馬 建鋒
  • Organizer: 日本土壌肥料学会2023年度愛媛大会
• [Presentation] アルミニウム処理によるART1のリン酸化と核蓄積 (2023)
  • Author(s): 小西範幸・馬 建鋒
  • Organizer: 日本土壌肥料学会2023年度愛媛大会
• [Presentation] 作物のミネラル輸送機構 (2023)
  • Author(s): 馬 建鋒
  • Organizer: 第119回日本土壌肥料学会関西支部講演会
  • Int'l Joint Research
• [Presentation] Mechanisms controlling polar localization of Si transporter in rice (2023)
  • Author(s): Ma, J. F. and Konishi, N.
  • Organizer: 19th International Workshop on Plant Membrane Biology
  • Int'l Joint Research / Invited
• [Presentation] Local distribution of manganese to leaf sheath is mediated by OsNramp5 in rice (2023)
  • Author(s): Huang, S., Yamaji, N., Konishi, N. and Ma, J. F.
  • Organizer: 19th International Workshop on Plant Membrane Biology
  • Int'l Joint Research
• [Presentation] イネのケイ素吸収を制御する長距離シグナルタンパク質の同定 (2023)
  • Author(s): 山地直樹・三谷奈見季・小西範幸・馬 建鋒
  • Organizer: 第64回日本植物生理学会年会
• [Presentation] イネケイ酸輸送体Lsi1の偏在制御に対するホスファチジン酸の関与の検討 (2023)
  • Author(s): 小西範幸・馬 建鋒
  • Organizer: 第64回日本植物生理学会年会
• [Presentation] OsMGT2 mediates the translocation and preferential distribution of magnesium in rice (2023)
  • Author(s): Huang, S., Yamaji, N. and Ma, J. F.
  • Organizer: 第64回日本植物生理学会年会
• [Presentation] Cadmium accumulation in cereal crops; from molecular mechanisms to breeding (2022)
  • Author(s): Ma, J. F.
  • Organizer: 54th Annual Conference of the German Society of Plant Nutrition
  • Int'l Joint Research / Invited
• [Presentation] OsZIP2 mediates xylem loading and preferential distribution of Zn in rice (2022)
  • Author(s): 黄 勝・山地直樹・馬 建鋒
  • Organizer: 日本土壌肥料学会2022年度東京大会
• [Presentation] Identification and functional characterization of a gene responsible for differential As accumulation in rice roots (2022)
  • Author(s): 王 珮同・馬 建鋒
  • Organizer: 日本土壌肥料学会2022年度東京大会
• [Presentation] イネケイ酸輸送体Lsi1の偏在は複数領域によって制御される (2022)
  • Author(s): 小西範幸・馬 建鋒
  • Organizer: 第63回日本植物生理学会年会
• [Presentation] イネのSIET4輸送体は細胞特異的ケイ素蓄積に関与する (2022)
  • Author(s): 三谷奈見季・山地直樹・吉岡佑真・宮地孝明・馬 建鋒
  • Organizer: 第63回日本植物生理学会年会
• [Presentation] 作物のミネラル輸送機構に関する研究 (2022)
  • Author(s): 馬 建鋒
  • Organizer: 第63回日本植物生理学会年会
  • Invited
• [Presentation] Node-localized transporters of Phosphorus Essential for Seed Development in Rice (2022)
  • Author(s): Che, J., Yamaji, N., Miyaji, T., Mitani-Ueno, N., Kato, Y., Shen, R. F. and Ma, J. F.
  • Organizer: 第63回日本植物生理学会年会
• [Presentation] イネにおけるホウ素吸収の制御機構 (2021)
  • Author(s): 黄 勝・山地直樹・小西範幸・邵 継鋒・馬 建鋒
  • Organizer: 日本土壌肥料学会2021年
• [Presentation] イネ由来ケイ酸チャネルによるケイ酸透過機構の構造基盤 (2021)
  • Author(s): 齊藤 恭紀, 三谷(上野) 奈見季, 斉藤 圭亮, 松木 謙悟, 黄 勝, 楊 霊麗, 山地 直樹, 石北 央, 沈 建仁, 馬 建鋒, 菅 倫寛
  • Organizer: 日本分子生物学会年会
• [Book] Marschner's Mineral Nutrition of Plants (Fourth Edition) (2023)
  • Author(s): Ma, J. F., Zhao, F-J., Rengel, Z., Cakmak, I.
  • Total Pages: 31
  • Publisher: Academic Press
• [Book] 植物のケイ酸チャネルの構造と機能 (2022)
  • Author(s): 齊藤 恭紀, 菅 倫寛
  • Total Pages: 2
  • Publisher: 日本結晶学会誌
• [Remarks]
  • URL: http://www.rib.okayama-u.ac.jp/plant.stress/index-j.html
• [Remarks]
  • URL: https://scholar.google.com/citations?user=kDZcBhkAAAAJ&hl=ja&oi=ao
• [Remarks]
  • URL: http://www.rib.okayama-u.ac.jp/plant.stress/index-j.html
• [Remarks]
  • URL: https://scholar.google.com/citations?user=kDZcBhkAAAAJ&hl=ja&oi=ao
• [Remarks]
  • URL: http://www.rib.okayama-u.ac.jp/plant.stress/index-j.html
• [Remarks]
  • URL: https://scholar.google.com/citations?user=kDZcBhkAAAAJ&hl=ja&oi=ao
• [Funded Workshop] International workshop on plant mineral transport (2024)