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Effect of Japanese green tea manufacturing process on structural attributes of tea leaves and biochemical properties of tea infusions

Research Project

Project/Area Number 21J10500
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section国内
Review Section Basic Section 41040:Agricultural environmental engineering and agricultural information engineering-related
Research InstitutionChiba University

Principal Investigator

秦 イ  千葉大学, 大学院園芸学研究科, 特別研究員(PD)

Project Period (FY) 2021-04-28 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2022: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2021: ¥800,000 (Direct Cost: ¥800,000)
Keywords製茶工程 / 緑茶 / 生物活性化合物 / 抗酸化活性
Outline of Research at the Start

Coarse kneading is the first process to destroy the structure of tea leaf at a certain temperature. Previous studies have shown that and coarse kneading and crumpling have the greatest impact on green tea quality during tea processing. However, there is no clear research on the impact of these two processing processes on tea quality and tea digestibility. This study aimed to investigate the effects of coarse kneading and crumpling conditions on bioactive compounds and antioxidant activities of tea infusions during in vitro digestion.

Outline of Annual Research Achievements

緑茶は抗酸化物質が豊富,慢性疾患の予防に重要な役割を果たしており, 多くの国で最も人気のある飲料の 1 つになっている. 機械製茶による煎茶 の製造工程は,基本的に,蒸熱,粗揉,揉捻,中揉,精揉,および乾燥の 6 操作で構成される.それら操作の条件を精密に調整することで,最終製品となる煎茶茶葉の品質向上が図られている.緑茶製品間の化学組成の違いは,一般的に茶葉の主産地と加工の異なる条件に起因する.
本研究は,加工条件(揉捻時間)が日本緑茶の機能品質に及ぼす影響を調査することを目的としている.茶葉の形態と微細構造を観察し,総ポリフェノール含有量 (TPC)と総抗酸化活性抗酸化活性(DPPH-ラジカル捕捉能と鉄還元抗 酸化力(FRAP)を茶葉と抽出液で評価した.茶葉を揉捻時間(15、30、60 分間)によって加工された.生産加工された茶葉の生物活性化合物(TPC)および抗酸化活性 (DPPH,FRAP)を評価し,そして,in vitro 人工消化実験における緑茶の生物活性化合物および抗酸化性の変化も調べた.異なる消化処理時間で消化液を抽出し,生物活性化合物の含有量抗酸化性を測定した.
強化された製造プロセスが,サンプルからの生物活性化合物の溶解および抗酸化活性の増加をもたらしたことを示した.茶浸出液における生物活性化合物と抗酸化活性は,人工体外消化を通して減少した.一方ですべてのサンプル の抗酸化性は胃と小腸の消化段階を通して安定しており,pH と消化酵素 の変化により小腸の消化が始まる間に明らかな値の変化を示した. したがって,加工条件がサンプル中の抗酸化物質の消化に耐える能力に影響を与えることを示唆している.

Research Progress Status

令和4年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和4年度が最終年度であるため、記入しない。

Report

(1 results)
  • 2022 Annual Research Report
  • Research Products

    (7 results)

All 2022

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (5 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Journal Article] Influence of manufacturing system scale for commercial Japanese green tea (Sencha) production on biochemical characteristics of tea leaf and infusion2022

    • Author(s)
      Qin Wei、Yamada Ryutaro、Araki Takuya、Ogawa Yukiharu
    • Journal Title

      Journal of Food Process Engineering

      Volume: 46 Issue: 1

    • DOI

      10.1111/jfpe.14185

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of digestive enzymes and pH on variation of bioavailability of green tea during simulated in vitro gastrointestinal digestion2022

    • Author(s)
      Qin Wei、Ketnawa Sunantha、Ogawa Yukiharu
    • Journal Title

      Food Science and Human Wellness

      Volume: 11 Issue: 3 Pages: 669-675

    • DOI

      10.1016/j.fshw.2021.12.024

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 製茶時の粗揉条件が緑茶の生理活性物質および抗酸化活性に及ぼす影響2022

    • Author(s)
      秦イ, 山田龍太郎, 荒木琢也, 小川幸春
    • Organizer
      美味技術学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Evaluation of Bioactive Compounds and Antioxidant Activity of Japanese Roasted Green Tea Infusion (Hojicha) During In-Vitro Gastrointestinal Digestion2022

    • Author(s)
      Wei Qin, Sunantha Ketnawa, Yukiharu Ogawa
    • Organizer
      The 4th International Conference on Agricultural and Agro-Industry ICAAI2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effect of rolling process on bioactive compounds and antioxidant activity of Japanese green tea infusion2022

    • Author(s)
      Wei Qin, Ryutaro Yamada, Takuya Araki, Yukiharu Ogawa
    • Organizer
      2022年度農業施設学会大会 (オンライン)
    • Related Report
      2022 Annual Research Report
  • [Presentation] Impact of processing conditions on bioactive compounds and antioxidant activity of Japanese green tea infusion2022

    • Author(s)
      Wei Qin, Ryutaro Yamada, Takuya Araki, Yukiharu Ogawa
    • Organizer
      第80回農業食料工学会年次大会 (オンライン)
    • Related Report
      2022 Annual Research Report
  • [Presentation] Study on processing condition and functional properties of Japanese green tea2022

    • Author(s)
      Wei Qin, Yukiharu Ogawa
    • Organizer
      International Symposium on Tea Science and Traditional Medicine Research (Online)
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited

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Published: 2021-05-27   Modified: 2024-03-26  

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