アニオン性ヘテロキレート配位子を用いた複製可能な金属錯体型人工DNA塩基対の開発
| Project/Area Number |
21J11332
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 国内 |
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| Review Section |
Basic Section 37010:Bio-related chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
HU Lingyun 東京大学, 理学系研究科, 特別研究員(DC2)
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| Project Period (FY) |
2021-04-28 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2022: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2021: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | DNA / Artificial DNA / Metal-mediated base pair / Cu(II) / DNA technology / Triazole-4-carboxylate / Artificial base pair / DNA nanotechnology |
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| Outline of Research at the Start |
Metal-mediated base pairs, consisting of two ligand-type artificial nucleobases bridged with a metal ion, have been widely used for constructing metal-responsive functional DNAs. This study aims to develop novel metal-mediated base pairs capable of efficient replication by DNA polymerases. After rational design and synthesis of ligand-type nucleobases, their base paring capability in the presence of metal ions will be studied and the enzymatic recognition capability will be examined with various polymerases. The selected base pair will be applied to SELEX for deriving unique functional DNAs.
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| Outline of Annual Research Achievements |
This study aims to develop suitable metal-mediated base pairs that can be enzymatically incorporated into the DNA. During my last year’s research, I have made several progresses as listed below:
1.Designed and synthesized several novel nucleobases based on the criteria concluded from the previous research and obtained oligonucleotides containing one or more such base pairs. Apart from the ImOC, which was reported previously, the TazC is a novel candidate.
2.Thermal melting analysis of DNA duplexes containing artificial nucleobases were conducted in the presence and absence of various metal ions, in which the TazC was found to form stable TazC-Cu(II)-TazC base pair.
3.ESI-TOF MS measurements were conducted on the TazC base pair, and the formation of the Cu(II)-mediated TazC base pair was confirmed.
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| Research Progress Status |
令和4年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和4年度が最終年度であるため、記入しない。
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Report
(2 results)
Research Products
(4 results)
