Studies on Nanometer-sized Artificial Basement Membranes for Cell Compartmentalization in Three-dimensional Tissues
| Project/Area Number |
21J12833
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 国内 |
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| Review Section |
Basic Section 90120:Biomaterials-related
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| Research Institution | Osaka University |
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Principal Investigator |
Zeng Jinfeng 大阪大学, 工学研究科, 特別研究員(PD)
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| Project Period (FY) |
2021-04-28 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2022: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2021: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | Artificial BMs / Compartmentalization / cation-dipole / surface charge / organized 3D tissue / ECM proteins / Cell migration / Tissue engineering |
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| Outline of Research at the Start |
In this study, A customized patterned artificial basement membrane based on a nanometer-sized multilayered film will be fabricated by the incorporation of layer-by layer assembly and bioprinting for organized 3D tissue construction.
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| Outline of Annual Research Achievements |
In this year, we evaluated the barrier effects of poly-L-lysine/dextran nanofilms (PLL/D NFs) constructed via the cation-dipole interaction to maintain compartmentalized cell co-culture of NHDF and HUVEC. PLL/D NFs exhibited comparable abilities to Col-IV/LM NFs in terms of cell adhesion, proliferation, and differentiation. However, it is worth noting that PLL/D NFs maintained compartmentalized cell co-culture for over two weeks, much longer than assembled nanofilms based on ECM proteins. Furthermore, the secretion of type IV collagen was observed under the confluent HUVEC monolayer, suggesting the re-construction of natural BMs in the in-vitro cultured tissues. The efficient barrier effects of PLL/D NFs provided a powerful support for constructing organized 3D tissues in vitro.
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| Research Progress Status |
令和4年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和4年度が最終年度であるため、記入しない。
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Report
(2 results)
Research Products
(3 results)
