| Project/Area Number |
21J14077
|
|---|
| Research Category |
Grant-in-Aid for JSPS Fellows
|
| Allocation Type | Single-year Grants |
|---|
| Section | 国内 |
|---|
| Review Section |
Basic Section 38010:Plant nutrition and soil science-related
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
卓 梦娜 東京大学, 農学生命科学研究科, 特別研究員(PD)
|
|---|
| Project Period (FY) |
2021-04-28 – 2023-03-31
|
| Project Status |
Completed (Fiscal Year 2022)
|
| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2022: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2021: ¥800,000 (Direct Cost: ¥800,000)
|
|---|
| Keywords | 窒素欠乏 / Dof転写因子 |
|---|
| Outline of Research at the Start |
The Dof1.7 encoding plant-specific Dof transcription factor was previously suggested to be involved in NIGT1-mediated nitrogen (N) starvation responses. The aim of this study is to elucidate the physiological functions of Dof1.7 in N starvation responses and related regulatory mechanisms.
|
| Outline of Annual Research Achievements |
In the first year, I have revealed that in model plant Arabidopsis, NIGT1 transcription factors, key regulators for N deficiency responses, directly repressed transcription of Dof1.7, and also revealed that knockout mutation of NIGT1 and Dof1.7 differentially affected the expression of three NRT2 genes encoding high-affinity nitrate transporters.
In the second year, I examined the transcriptional relationship between Dof1.7 and the three NRT2 genes by ChIP and co-transfection assay and revealed that Dof1.7 directly activated the NRT2.5 promoter and indirectly activated the NRT2.4 promoter with the presence of NIGT1.1. I have completed a manuscript of this series of research and am preparing it for publication in a high-rank international journal.
|
| Research Progress Status |
令和4年度が最終年度であるため、記入しない。
|
| Strategy for Future Research Activity |
令和4年度が最終年度であるため、記入しない。
|
