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Identification of the dopaminergic projection pathways responsible for courtship vocalizations and sexual motivation in male mice

Research Project

Project/Area Number 21K03144
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 10040:Experimental psychology-related
Research InstitutionKagoshima University

Principal Investigator

Kanno Kouta  鹿児島大学, 法文教育学域法文学系, 准教授 (80722470)

Co-Investigator(Kenkyū-buntansha) 奥野 浩行  鹿児島大学, 医歯学域医学系, 教授 (80272417)
Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2023: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2022: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords超音波発声 / 音声コミュニケーション / マウス / 性行動 / 求愛 / 社会行動 / 性的不応期
Outline of Research at the Start

雄マウスが超音波で発する求愛発声に対して、[超音波録音]・[ビデオ録画]・[神経活動の記録]と操作を同期して行うことで「求愛発声の多さと音節の複雑さには、性的動機づけの強さとドーパミン(DA)神経活動が表出されている」ことを一括・統合して示す。さらに、射精後の性的不応期には求愛発声も見られなくなることに着目し、性的不応期に関与するDA神経投射のうち、どの回路が求愛発声を制御しているかを同定する。この不応期のDAシグナルは、性的動機づけのみならず性的身体反応も担うが、本研究により、これらDA神経系のどの回路が発声と動機づけを機能分担しているか、明らかにする。

Outline of Final Research Achievements

The purpose of this study was to clarify the relationship between the emotional state expressed in ultrasonic courtship vocalizations of mice and dopaminergic activity. We hypothesized that males, which are highly sexually motivated, frequently exhibit characteristic vocalizations when they encounter females and that dopaminergic neurons are activated. We attempted to test these hypotheses by manipulating and imaging neural activity, but were unable to establish such experimental systems and could not test the hypotheses. In contrast, the behavioral analysis was successful. We developed a method to quantify the acoustic features that can be described as the "rise" of the vocalizations when sexual motivation is heightened. Furthermore, we demonstrated the possibility of observing such a rise among multiple individuals.

Academic Significance and Societal Importance of the Research Achievements

私はこれまでに一貫してマウス超音波発声の内容が示す生物学的意義を研究してきた。このことにより、近年、生物医学・神経科学分野では、コミュニケーションに障害が見られる発達障害のモデルマウスにおいて超音波発声が頻繁に解析されている。本研究の成果も、そのような病態モデルマウスの行動表現型に対する解析・解釈に今後貢献しうる。高精度な実験・解析手法が蓄積されることにより、動物実験心理学や神経科学分野の研究者に広く技術を提供することにもつながる。また、本研究は、感情表現にも繋がる情動の表出とそのメカニズムを探るもので、そのような基礎研究の進展に貢献する意義を持つ。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (21 results)

All 2024 2023 2022 2021 Other

All Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 5 results) Presentation (8 results) (of which Int'l Joint Research: 4 results,  Invited: 2 results) Remarks (6 results)

  • [Journal Article] 特集 脳と個性 Ⅲ."個性"の実験的理解 動物の鳴き声の"個性"2024

    • Author(s)
      菅野 康太
    • Journal Title

      生体の科学

      Volume: 75 Issue: 1 Pages: 69-73

    • DOI

      10.11477/mf.2425201817

    • ISSN
      0370-9531, 1883-5503
    • Year and Date
      2024-02-15
    • Related Report
      2023 Annual Research Report
  • [Journal Article] Chronic exposure of female mice to selective serotonin reuptake inhibitors during lactation induces vocal behavior deficits in pre-weaned offspring2023

    • Author(s)
      Lan Ziguo、Tachibana Ryosuke O.、Kanno Kouta
    • Journal Title

      Pharmacology Biochemistry and Behavior

      Volume: 230 Pages: 173606-173606

    • DOI

      10.1016/j.pbb.2023.173606

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Acoustic Properties and Biological Significance of Ultrasonic Vocalizations in Rodents: Emotional Expressions.2023

    • Author(s)
      Okabe Shota、Kanno Kouta
    • Journal Title

      In: Seki, Y. (eds) Acoustic Communication in Animals (Chapter 10, pp153-173). Springer, Singapore.

      Volume: - Pages: 153-173

    • DOI

      10.1007/978-981-99-0831-8_10

    • ISBN
      9789819908301, 9789819908318
    • Related Report
      2023 Annual Research Report
  • [Journal Article] Rodent ultrasonic vocalizations as emotional expressions: Vocal communication in mice and rats2022

    • Author(s)
      岡部 祥太、菅野 康太
    • Journal Title

      THE JOURNAL OF THE ACOUSTICAL SOCIETY OF JAPAN

      Volume: 79 Issue: 1 Pages: 41-48

    • DOI

      10.20697/jasj.79.1_41

    • ISSN
      0369-4232, 2432-2040
    • Year and Date
      2022-12-25
    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Advanced paternal age diversifies individual trajectories of vocalization patterns in neonatal mice2022

    • Author(s)
      Mai Lingling、Inada Hitoshi、Kimura Ryuichi、Kanno Kouta、Matsuda Takeru、Tachibana Ryosuke O.、Tucci Valter、Komaki Fumiyasu、Hiroi Noboru、Osumi Noriko
    • Journal Title

      iScience

      Volume: 25 Issue: 8 Pages: 104834-104834

    • DOI

      10.1016/j.isci.2022.104834

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Acoustic camera system for measuring ultrasound communication in mice2022

    • Author(s)
      Matsumoto Jumpei、Kanno Kouta、Kato Masahiro、Nishimaru Hiroshi、Setogawa Tsuyoshi、Chinzorig Choijiljav、Shibata Tomohiro、Nishijo Hisao
    • Journal Title

      iScience

      Volume: 25 Issue: 8 Pages: 104812-104812

    • DOI

      10.1016/j.isci.2022.104812

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Acoustic camera system for measuring ultrasound communication in mice2021

    • Author(s)
      Matsumoto Jumpei、Kanno Kouta、Kato Masahiro、Nishimaru Hiroshi、Setogawa Tsuyoshi、Chinzorig Choijiljav、Shibata Tomohiro、Nishijo Hisao
    • Journal Title

      bioRxiv(プレプリント)

      Volume: -

    • DOI

      10.1101/2021.12.12.468927

    • Related Report
      2021 Research-status Report
    • Open Access
  • [Presentation] 性特異行動としてマウス超音波発声2023

    • Author(s)
      菅野康太
    • Organizer
      第46回日本神経科学大会シンポジウム「性差に着目した神経科学研究最前線」
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 情動表出としてのマウス超音波発声 -その発声の種類と個体差-2023

    • Author(s)
      菅野康太
    • Organizer
      日本音響学会第149回(2023年春季)研究発表会「スペシャルセッション(ヒトと動物の情動発声と声の社会性)」
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] Mice emit ultrasonic vocalizations in the context with a running wheel2022

    • Author(s)
      栗脇開世、菅野康太
    • Organizer
      日本動物心理学会 第82回大会
    • Related Report
      2022 Research-status Report
  • [Presentation] Chronic maternal SSRI exposure during the prenatal and/or postanal period induced vocal behavior deficits and affected serotonergic neurogenesis in the mouse offspring2022

    • Author(s)
      Ziguo Lan, Noriko Osumi, Kouta Kanno
    • Organizer
      FENS Forum 2022, The Federation of European Neuroscience Societies
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] USVCAM: acoustic camera system for measuring ultrasound communication in mice(USVCAM:マウス超音波発声を計測する超音波カメラ)2022

    • Author(s)
      Kouta Kanno, Jumpei Matsumoto, Masahiro Kato, Hiroshi Nishimaru, Tsuyoshi Setogawa, Choijiljav Chinzorig, Tomohiro Shibata, Hisao Nishijo
    • Organizer
      NEURO2022(第45回日本神経科学大会・第65回日本神経化学会大会・第32回日本神経回路学会大会)
    • Related Report
      2022 Research-status Report
  • [Presentation] マウス超音波発声による情動状態とその個体差の評価2022

    • Author(s)
      菅野康太
    • Organizer
      シンポジウム2「感情の評価:多彩な方法を極める」:第40回日本生理心理学会大会・日本感情心理学会第30回大会 合同大会2022
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] マウスの快情動を定量化する方法2021

    • Author(s)
      桑木共之、菅野康太
    • Organizer
      第44回神経科学大会(日本神経科学学会)
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] 母胎内における妊娠期・授乳期の長期的SSRI曝露は仔マウスの音声行動およびセロトニンニュー ロン形成に影響する2021

    • Author(s)
      蘭子国、大隅典子、菅野康太
    • Organizer
      第44回神経科学大会(日本神経科学学会)
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Remarks] 研究室ウェブサイト

    • URL

      https://cannonolab.com

    • Related Report
      2023 Annual Research Report
  • [Remarks] 鹿児島大学研究者総覧

    • URL

      https://ris.kuas.kagoshima-u.ac.jp/html/100006503_ja.html#item_ronbn_2

    • Related Report
      2023 Annual Research Report
  • [Remarks] Researchmap

    • URL

      https://researchmap.jp/can-no/?lang=ja

    • Related Report
      2023 Annual Research Report 2022 Research-status Report 2021 Research-status Report
  • [Remarks] Vocal Communication Japan

    • URL

      https://sites.google.com/view/vocalcommuj/home

    • Related Report
      2023 Annual Research Report 2022 Research-status Report
  • [Remarks] 菅野研究室HP

    • URL

      https://cannonolab.com

    • Related Report
      2022 Research-status Report 2021 Research-status Report
  • [Remarks] 鹿児島大学研究者総覧

    • URL

      http://ris.kuas.kagoshima-u.ac.jp/html/100006503_ja.html

    • Related Report
      2022 Research-status Report 2021 Research-status Report

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Published: 2021-04-28   Modified: 2025-01-30  

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