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Fabrication of artificial muscles from biomolecular motors and DNA origami nanostructures

Research Project

Project/Area Number 21K04846
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 28040:Nanobioscience-related
Research InstitutionHokkaido University

Principal Investigator

コビル アリフ  北海道大学, 理学研究院, 特任講師 (10724867)

Project Period (FY) 2021-04-01 – 2023-03-31
Project Status Discontinued (Fiscal Year 2022)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2023: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2022: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsArtificial muscles / Biomolecular motors
Outline of Research at the Start

The purpose is mimic nature’s strategy and create artificial muscles in a biomimetic approach. Recently, I have created artificial sarcomere that exhibited dynamic global contraction. Here, I will expand my research to create an artificial muscle. I will demonstrate dynamic relaxation of the artificial sarcomere after contraction, control the kinetics of contraction and relaxation of artificial sarcomere and control the hierarchical organization of the sarcomere networks in three dimensions to create artificial muscles.

Outline of Annual Research Achievements

This project was aimed at fabricating artificial muscles through fusion of biomolecular motors (microtubules, kinesin) and DNA origami nanostructures. The construction of artificial muscles consists of two phases: dynamic contraction of a global microtubule network and subsequent relaxation of the contracted network. The dynamic contraction of a global microtubule network has been achieved successfully by using microtubules, six helix bundle DNA origami nanostructures, and tetrameric kinesin linkers. Moreover, by reducing the number of kinesin motors interacting with microtubules from four to three, we are also able to slow down the kinetics of contraction of the global microtubule network significantly. However, relaxation of the contracted microtubule network has still remained a challenge. Use of a photocleavable linker DNA has facilitated relaxation of the contracted microtubule network to some extent. In future, further attempts are required to accelerate this relaxation process with an ability to repeatedly contract and relax similar to that of muscles in living organisms.

Report

(2 results)
  • 2022 Annual Research Report
  • 2021 Research-status Report
  • Research Products

    (15 results)

All 2023 2022 2021

All Journal Article (9 results) (of which Int'l Joint Research: 9 results,  Peer Reviewed: 9 results,  Open Access: 7 results) Presentation (6 results) (of which Int'l Joint Research: 3 results,  Invited: 4 results)

  • [Journal Article] Effects of three microtubule-associated proteins (MAP2, MAP4, and Tau) on microtubules’ physical properties and neurite morphology2023

    • Author(s)
      Kohei Nishida, Kosuke Matsumura, Miki Tamura, Takuto Nakamichi, Keiya Shimamori, Masahiro Kuragano, Arif Md. Rashedul Kabir, Akira Kakugo, Susumu Kotani, Naoki Nishishita, and Kiyotaka Tokuraku
    • Journal Title

      Scientific Reports

      Volume: 13 Issue: 1 Pages: 1-12

    • DOI

      10.1038/s41598-023-36073-9

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 3D Structure of Ring-shaped Microtubule Swarms Revealed by High-speed Atomic Force Microscopy2023

    • Author(s)
      Rashid Mst. Rubaya、Ganser Christian、Akter Mousumi、Nasrin Syeda Rubaiya、Kabir Arif Md. Rashedul、Sada Kazuki、Uchihashi Takayuki、Kakugo Akira
    • Journal Title

      Chemistry Letters

      Volume: 52 Issue: 2 Pages: 100-104

    • DOI

      10.1246/cl.220491

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Controlling the Rigidity of Kinesin-Propelled Microtubules in an In Vitro Gliding Assay Using the Deep-Sea Osmolyte Trimethylamine N-Oxide2022

    • Author(s)
      Kabir Arif Md. Rashedul、Munmun Tasrina、Hayashi Tomohiko、Yasuda Satoshi、Kimura Atsushi P.、Kinoshita Masahiro、Murata Takeshi、Sada Kazuki、Kakugo Akira
    • Journal Title

      ACS Omega

      Volume: 7 Issue: 4 Pages: 3796-3803

    • DOI

      10.1021/acsomega.1c06699

    • Related Report
      2022 Annual Research Report 2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cooperative cargo transportation by a swarm of molecular machines2022

    • Author(s)
      Akter M.、Keya J. J.、Kayano K.、Kabir A. M. R.、Inoue D.、Hess H.、Sada K.、Kuzuya A.、Asanuma H.、Kakugo A.
    • Journal Title

      Science Robotics

      Volume: 7 Issue: 65

    • DOI

      10.1126/scirobotics.abm0677

    • Related Report
      2022 Annual Research Report 2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Fluctuation in the Sliding Movement of Kinesin-Driven Microtubules is Regulated Using the Deep-Sea Osmolyte Trimethylamine N-Oxide2022

    • Author(s)
      Arif Md. Rashedul Kabir, Tasrina Munmun, Kazuki Sada, and Akira Kakugo
    • Journal Title

      ACS Omega

      Volume: 7 Issue: 22 Pages: 18597-18604

    • DOI

      10.1021/acsomega.2c01228

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Kinesin motors driven microtubule swarming triggered by UV light2022

    • Author(s)
      Ishii Satsuki、Akter Mousumi、Murayama Keiji、Kabir Arif Md. Rashedul、Asanuma Hiroyuki、Sada Kazuki、Kakugo Akira
    • Journal Title

      Polymer Journal

      Volume: 54 Issue: 12 Pages: 1501-1507

    • DOI

      10.1038/s41428-022-00693-1

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Collision-induced torque mediates the transition of chiral dynamic patterns formed by active particles2022

    • Author(s)
      Hiraiwa Tetsuya、Akiyama Ryo、Inoue Daisuke、Kabir Arif Md. Rashedul、Kakugo Akira
    • Journal Title

      Physical Chemistry Chemical Physics

      Volume: 24 Issue: 47 Pages: 28782-28787

    • DOI

      10.1039/d2cp03879j

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Generation of stable microtubule superstructures by binding of peptide-fused tetrameric proteins to inside and outside2022

    • Author(s)
      HIROSHI INABA, YURINA SUEKI, MUNEYOSHI ICHIKAWA, ARIF MD. RASHEDUL KABIR, TAKASHI IWASAKI, HIDEKI SHIGEMATSU, AKIRA KAKUGO, KAZUKI SADA, TOMOYA TSUKAZAKI, and KAZUNORI MATSUURA
    • Journal Title

      Science Advances

      Volume: 8

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Controlling Collective Motion of Kinesin-Driven Microtubules via Patterning of Topographic Landscapes2021

    • Author(s)
      Araki Shunya、Beppu Kazusa、Kabir Arif Md. Rashedul、Kakugo Akira、Maeda Yusuke T.
    • Journal Title

      Nano Letters

      Volume: 21 Issue: 24 Pages: 10478-10485

    • DOI

      10.1021/acs.nanolett.1c03952

    • Related Report
      2022 Annual Research Report 2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Scaling-up biomolecular motors using DNA nanotechnology2022

    • Author(s)
      Arif Md. Rashedul Kabir
    • Organizer
      45th Annual Meeting of the Australian Society for Biophysics
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Utilization of biomolecular motors as actuators for a minimal artificial brain2022

    • Author(s)
      Arif Md. Rashedul Kabir
    • Organizer
      Cell Synth14.0 meeting
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Scaling up motor proteins using DNA technology for utilizing as molecular actuators2022

    • Author(s)
      Arif Md. Rashedul Kabir
    • Organizer
      Public Symposium on Molecular Cybernetics
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] SCALING-UP BIOMOLECULAR MOTORS USING DNA NANOTECHNOLOGY2021

    • Author(s)
      Arif Md. Rashedul Kabir, Akinori Kuzuya, Akira Kakugo
    • Organizer
      45th Annual Meeting 2021, Australian Society for Biophysics
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] Utility of biomolecular motors for a minimal artificial brain2021

    • Author(s)
      Arif Md. Rashedul Kabir
    • Organizer
      Cell Synth14.0 meeting, 2021
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] Sensing surface mechanical deformation of soft materials using biomolecular motors2021

    • Author(s)
      Arif Md. Rashedul Kabir
    • Organizer
      2021 MIRAI2.0 Workshop in Materials Science
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2021-04-28   Modified: 2023-12-25  

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