| Project/Area Number |
21K05044
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 33010:Structural organic chemistry and physical organic chemistry-related
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| Research Institution | Shibaura Institute of Technology |
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Principal Investigator |
RICHARDS GARY 芝浦工業大学, 工学部, 准教授 (00708620)
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| Co-Investigator(Kenkyū-buntansha) |
堀 顕子 芝浦工業大学, 工学部, 教授 (90433713)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2023: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2022: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | Pyrazinacenes / Near Infrared / Fluorescence / Cellular Imaging / Bioimaging / Acenes / Azaacenes / Near Infrared Emitters / REDOX / Cellular imaging / Near infrared absorption |
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| Outline of Research at the Start |
Chromophore-based bioimaging techniques rely on the availability of dyes that are emissive in regions of the electromagnetic spectrum not obscured by absorptive processes of biomolecules or water. Thus, dyes emitting in the near-infrared transparency window region of tissues from 650 to 900 nm are useful for imaging. This project aims to develop a new class of highly efficient near infra-red emitting materials based on extended pyrazinacenes - molecules built with linearly fused pyrazine units.
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| Outline of Final Research Achievements |
An extended pyrazinacenes consisting of 7 rectilinearly fused pyrazine units appended with hydrophilic triethylene glycol groups was synthesised. The synthesis of the compound and its purification was optimized. The compound was used in cellular imaging studies in the near-infrared region. Interestingly, this new fluorescent dye can distinguish DNA and RNA cellular structures by simply changing excitation and observation wavelengths.
Redox-active pyrazinacenes consisting of four or five fused pyrazine rings appended with electron-donating triphenylamine groups were synthesized. These compounds exhibit redox-coupled fluorescence switching between visible and near infrared or shortwave infrared regions. We are now developing related compounds as new types of probes for biological and cellular redox states.
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| Academic Significance and Societal Importance of the Research Achievements |
細胞イメージングは、生物学、医学、薬学研究において非常に重要な分野である。近赤外領域に活性を持つ蛍光分子を用いることで、細胞を傷つけることなく、長期間にわたって細胞のプロセスを研究することが可能になる。近赤外領域に活性を持つ蛍光色素の多くは、蛍光量子収率の低さや光退色という問題を抱えている。本研究では、これらの問題を克服する新しいタイプの近赤外蛍光分子の開発を目指す。
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