Spatiotemporal mapping of gene expression to reveal mechanisms of cellular differentiation in E. coli biofilms
| Project/Area Number |
21K05341
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 38020:Applied microbiology-related
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| Research Institution | Kyoto University |
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Principal Investigator |
Robert Martin 京都大学, 薬学研究科, 特定准教授 (90365487)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2023: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2022: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2021: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | biofilm / E. coli / imaging / gene expression / proteome analysis / cell motility / acid fermentation / imaging system / proteome / surface properties / nutrients / differentiation / Escherichia coli |
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| Outline of Research at the Start |
Bacteria can form biofilms having unique and emergent biological properties. To explore these properties we will quantify gene expression and metabolic activity in E. coli biofilms using time-lapse fluorescence imaging of gene expression, and spatio-temporal mapping of the cellular proteome.
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| Outline of Annual Research Achievements |
1) We completed the spatial analysis of protein expression. The results revealed specific enrichment of biological functions into different parts of the biofilm and hints of functional differentiation
2) We designed, developed and benched-marked a new automated and multi-mode imaging system to monitor the growth of biofilms, their cell density, surface morphology, and fluorescence in two different colors
3) During growth of biofilms on LB containing glucose we revealed a spatio-temporal metabolic shift that influences growth rate and three-dimensional structure.
4) We found that an expression of an uncharacterized cell-death related gene can affect biofilm 3D structure. This phenomenon seems to occur in a calcium-dependent process and affects cell density and biofilm morphology.
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Report
(3 results)
Research Products
(13 results)
