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How does supercooling capability of grape buds change during a deacclimation process?

Research Project

Project/Area Number 21K05555
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 39030:Horticultural science-related
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

Kasuga Jun  帯広畜産大学, 畜産学部, 准教授 (40451421)

Co-Investigator(Kenkyū-buntansha) 鈴木 卓  北海道大学, 農学研究院, 教授 (30196836)
高橋 大輔  埼玉大学, 理工学研究科, 助教 (20784961)
Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2023: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsブドウ / 耐寒性 / 脱馴化 / 冬芽 / 過冷却 / 組織構造 / 再馴化
Outline of Research at the Start

越冬性の植物が、厳冬期以降の気温の上昇によって耐寒性を低下させることを脱馴化と言う。寒冷地における果樹栽培では、この脱馴化の時期にしばしば凍霜害が発生する。本研究では、ブドウを用いて、最も凍霜害を受けやすい器官である冬芽が脱馴化過程で耐寒性を失うメカニズムの解明を目指す。ブドウの冬芽は、内部の水分を過冷却させて凍結を回避し、この過冷却した水分の凍結が起こる温度によりその耐寒性が決まっている。そこで、脱馴化過程で起こる冬芽内部の水分の融点(内部での氷核形成温度に影響)と冬芽の基部の組織の構造変化(外部からの凍結の侵入防止に関与)を調べ、冬芽の過冷却能力を低下させる因子の特定を試みる。

Outline of Final Research Achievements

Winter buds of grapevines adapt subfreezing temperatures by supercooling. In this study, we attempted to elucidate the mechanism by which grape buds lose their freezing resistance in early spring. Winter buds of 'Yamasachi' variety grown in the Tokachi region gradually declined their freezing resistance from late February. Then, a temporal increase of the decline rate was observed in early April, and the buds lost their freezing resistance in early May at the same time with budburst. We observed that the initiation of xylem water transport from twigs to bud primordia and the formation of new vessels with larger diameter in buds occurred in early April and during budburst, respectively. These results suggest that enlargement of the ice propagation pathway from the outside to the inside of winter buds led to a stepwise decrease in freezing resistance of grape buds in early spring.

Academic Significance and Societal Importance of the Research Achievements

ブドウの冬芽が芽吹きの時期に耐寒性を失うことは良く知られており、寒冷地域で栽培されるブドウでは、しばしば晩霜害が発生する。ブドウの冬芽が晩霜害を受ける際は、枝から冬芽へと凍結が伝播することは過去に報告されていたが、芽吹き前後で冬芽の耐寒性が劇的に変化する機構については未解明であった。本研究の結果は、道管を通る冬芽内への凍結の伝播しやすさの変化が冬芽の耐寒性喪失に関与することを強く示した。さらに、冬芽と隣接する枝の組織に、未同定ではあるが、凍結の伝播しやすさを決める構造物が存在することを確認した。この構造物を同定し、その特性を変えることができれば、ブドウの晩霜害の低減につながる可能性がある。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (20 results)

All 2024 2023 2022 2021 Other

All Int'l Joint Research (3 results) Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 1 results) Presentation (8 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Remarks (4 results)

  • [Int'l Joint Research] ウィスコンシン大学マディソン校(米国)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] ウィスコンシン大学マディソン校(米国)

    • Related Report
      2022 Research-status Report
  • [Int'l Joint Research] ウィスコンシン大学マディソン校(米国)

    • Related Report
      2021 Research-status Report
  • [Journal Article] Changes in Freezing Resistance of Grape Buds under The Snow after Mid-Winter2023

    • Author(s)
      春日 純、西 龍一郎、廻渕 凌匠
    • Journal Title

      Cryobiology and Cryotechnology

      Volume: 69 Issue: 2 Pages: 57-60

    • DOI

      10.20585/cryobolcryotechnol.69.2_57

    • ISSN
      1340-7902, 2424-1555
    • Year and Date
      2023-11-15
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vessel development in compound buds of interspecific hybrid grape induced by artificial deacclimation treatments2023

    • Author(s)
      Kasuga Jun、Kawase Ayumi、Kamigaki Rika、Mihara Yuka、Kondoh Daisuke
    • Journal Title

      Physiologia Plantarum

      Volume: 175 Issue: 3

    • DOI

      10.1111/ppl.13940

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Freezing resistance and behavior of winter buds and canes of wine grapes cultivated in northern Japan2021

    • Author(s)
      Horiuchi Reiko、Arakawa Keita、Kasuga Jun、Suzuki Takashi、Jitsuyama Yutaka
    • Journal Title

      Cryobiology

      Volume: 101 Pages: 44-51

    • DOI

      10.1016/j.cryobiol.2021.06.004

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] 冬季の不織布資材による被覆がブドウの周囲温度と休眠芽の凍結抵抗性に与える影響2021

    • Author(s)
      春日純、兼谷颯斗、東億、金谷真稀
    • Journal Title

      日本ブドウ・ワイン学会誌

      Volume: 32 Pages: 3-14

    • NAID

      40022706320

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Responses of the Plant Cell Wall to Sub-Zero Temperatures: A Brief Update2021

    • Author(s)
      Takahashi Daisuke、Willick Ian R、Kasuga Jun、Livingston III David P
    • Journal Title

      Plant and Cell Physiology

      Volume: 62 Issue: 12 Pages: 1858-1866

    • DOI

      10.1093/pcp/pcab103

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 高耐寒性種間交雑ブドウの冬芽の凍結環境下における脱水特性2024

    • Author(s)
      春日純、坂原詩織
    • Organizer
      第65回日本植物生理学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] ブドウ冬芽の初春の耐寒性決定に関与する道管内バリア構造2023

    • Author(s)
      廻渕凌匠、春日純
    • Organizer
      北海道園芸研究談話会2023年度研究発表会
    • Related Report
      2023 Annual Research Report
  • [Presentation] ブドウの冬芽における脱馴化の進行による再馴化能力の変化2023

    • Author(s)
      村上未葉、松田愉愼、西龍一郎、春日純
    • Organizer
      北海道園芸研究談話会2023年度研究発表会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 厳冬期以降のブドウの冬芽の雪中での耐寒性変化2023

    • Author(s)
      春日純、西龍一郎、廻渕凌匠
    • Organizer
      第68回低温生物工学会大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 北海道で栽培される醸造用ブドウの冬芽の凍結環境下における脱水特性2022

    • Author(s)
      坂原詩織、Amaya Atucha、春日純
    • Organizer
      北海道園芸研究談話会2022年度研究発表会(オンライン)
    • Related Report
      2022 Research-status Report
  • [Presentation] 摘葉処理がブドウ品種‘山幸’の果汁品質に与える影響2022

    • Author(s)
      春日純、河瀬肖美、神垣里夏、近藤大輔
    • Organizer
      第67回低温生物工学会大会(オンライン)
    • Related Report
      2022 Research-status Report
  • [Presentation] Effects of unwoven fabric sheet cover on freezing resistance of grape buds during winter2021

    • Author(s)
      Kaneya Hayato、Azuma Hakaru、Kanaya Maki、Kasuga Jun
    • Organizer
      12th International Plant Cold Hardiness Seminar
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] LECTURE 6: Deep supercooling: freezing avoidance mechanisms in woody plants2021

    • Author(s)
      Kasuga Jun
    • Organizer
      12th IPCHS Course (Pre-recorded online lecture)
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Remarks] 帯広畜産大学植物生理学研究室ホームページ

    • URL

      https://univ.obihiro.ac.jp/~plantphysiol/index.html

    • Related Report
      2023 Annual Research Report
  • [Remarks] 帯広畜産大学植物生理学研究室北方ぶどう情報室

    • URL

      https://univ.obihiro.ac.jp/~plantphysiol/grape/index.html

    • Related Report
      2023 Annual Research Report
  • [Remarks] 帯広畜産大学植物生理学研究室ホームページ

    • URL

      http://univ.obihiro.ac.jp/~plantphysiol/index.html

    • Related Report
      2022 Research-status Report 2021 Research-status Report
  • [Remarks] 帯広畜産大学植物生理学研究室北方ぶどう情報室

    • URL

      http://univ.obihiro.ac.jp/~plantphysiol/grape/index.html

    • Related Report
      2022 Research-status Report 2021 Research-status Report

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Published: 2021-04-28   Modified: 2025-01-30  

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