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Characterization of the causative gene of Csc1, an self-compatible locus of Chrysanthemum seticuspe

Research Project

Project/Area Number 21K05564
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 39030:Horticultural science-related
Research InstitutionKochi University

Principal Investigator

NAKANO Michiharu  高知大学, 教育研究部自然科学系農学部門, 准教授 (40705159)

Co-Investigator(Kenkyū-buntansha) 草場 信  広島大学, 統合生命科学研究科(理), 教授 (20370653)
Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2023: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2022: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2021: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords野生ギク / 自家不和合性 / ポジショナルクローニング / 自家和合性 / キクタニギク / ゲノム解析 / キク
Outline of Research at the Start

キクタニギクに見出された自家和合性遺伝子座Csc1のポジショナルクローニングを進める。これまでの解析で候補とされたSLPK遺伝子を中心に研究を進めることで自家和合性の機構解明を目指し、栽培ギク等を用いた機能検定を行うことで応用の可能性を探る。本研究により、アブラナ科以外では詳細な解析が進んでいない胞子型自家不和合性について、キク科で初めてのメカニズム解明につながることが期待される。

Outline of Final Research Achievements

Genetic analysis of important traits in Chrysanthemum has not progressed due to polyploidy and self-incompatibility. Our group isolated the self-compatible mutant line AEV02 from the genetic resources of Chrysanthemum seticuspe. To determine the molecular function of Csc1, a single recessive locus that determines self-compatibility in AEV02, we performed genetic analysis using the recently determined genome sequence of Gojo-0. As a result of genetic analysis using segregating populations, the candidate genomic region was redefined but 862 kb of the previously narrowed genomic region was excluded. In the redefined genomic region, an LTR-type retrotransposon was inserted into an LRK gene, which was highly expressed in the style. We considered it a candidate for an important causative gene and are undergoing transformation experiments. In addition, genetic analysis of whether the Csc1 gene is an S gene or a modifier gene revealed that Csc1 acts as an S gene.

Academic Significance and Societal Importance of the Research Achievements

Csc1遺伝子座はキク属において初めての自家和合性遺伝子であり、その解明はキク育種への貢献だけでなくキク科の自家不和合性機構の解明につながると期待される。本研究でCsc1の理解が進んだが、特にCsc1がS遺伝子として作用することが明らかとなったことは、植物最大の科であるキク科における初めての自家不和合性メカニズムの解明に向けて重要な知見となることが期待される。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (1 results)

All 2021

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results)

  • [Journal Article] A chromosome-level genome sequence of Chrysanthemum seticuspe, a model species for hexaploid cultivated chrysanthemum2021

    • Author(s)
      Nakano Michiharu、Hirakawa Hideki、Fukai Eigo、Toyoda Atsushi、Kajitani Rei、Minakuchi Yohei、Itoh Takehiko、Higuchi Yohei、Kozuka Toshiaki、Bono Hidemasa、Shirasawa Kenta、Shiraiwa Ippei、Sumitomo Katsuhiko、Hisamatsu Tamotsu、Shibata Michio、Isobe Sachiko、Taniguchi Kenji、Kusaba Makoto
    • Journal Title

      Communications Biology

      Volume: 4 Issue: 1 Pages: 1167-1167

    • DOI

      10.1038/s42003-021-02704-y

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access

URL: 

Published: 2021-04-28   Modified: 2025-01-30  

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