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Development of conservation methods using seed-sawing of endangered orchid species

Research Project

Project/Area Number 21K05645
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 39060:Conservation of biological resources-related
Research InstitutionFukushima University

Principal Investigator

Yumi Yamashita  福島大学, 共生システム理工学類, 客員准教授 (30792543)

Co-Investigator(Kenkyū-buntansha) 遊川 知久  独立行政法人国立科学博物館, 植物研究部, グループ長 (50280524)
辻田 有紀  佐賀大学, 農学部, 准教授 (80522523)
山下 俊之  奥羽大学, 薬学部, 教授 (90192400)
Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2023: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2022: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2021: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsラン科 / 共生菌 / 自生地播種試験 / 保全 / 発芽生態 / ラン科植物 / 自生地播種 / 種子発芽 / 絶滅危惧種 / 生息域内保全
Outline of Research at the Start

日本産ラン科植物の約60%は絶滅危惧種であり、保全技術の確立が急務である。しかし、散布された微細種子や幼若個体の地中での観察が困難で、保全に不可欠な種子繁殖特性や幼若期の共生菌多様性がほとんど明らかになっていない。この「ブラックボックス」を解明するために本研究では、ラン科の主要な生育立地である林床、草地、湿地、樹上に自生する種を選抜し、それぞれの立地に適した播種および幼若個体調査の技術体系を構築する。さらに、微小な幼若個体からターゲットとする共生菌を高精度で同定する手法を確立する。一連の試験により複数種の絶滅危惧種の発芽生態を解明し、生息域内保全・野生復帰の成功率を格段に向上させる。

Outline of Final Research Achievements

About 60% of Japanese orchid species are listed as endangered, and there is an urgent need to establish conservation techniques. However, because orchid seeds are minute, it is difficult to observe dispersed seeds and juvenile individuals in the ground, and seed reproductive characteristics and juvenile symbiotic fungal diversity, which are essential for conservation, have not been clarified for most species. To elucidate these “black boxes,” it is necessary to establish methods for continuous observation of early growth in a variety of locations. As a result of conducting authigenic seeding tests using bags of seeds in the following representative orchid locations: 1. forest floor, 2. wetlands, and 3. tree tops, we were able to establish a method for observing the initial growth of orchid plants in representative locations and analyze the diversity of symbiotic fungi.

Academic Significance and Societal Importance of the Research Achievements

本研究の成果は、ラン科のみならず微細種子・胞子を散布体とする植物(ツツジ科、リン ドウ科、シダ植物等)の生物多様性、生態学、保全に以下のように貢献する。1.微細種子・胞子をもつ植物でこれまで「ブラックボックス」だった発芽、初期成長に適した光、温度、水、栄養などの特性が解明でき、植物体(特に地下部)の形態、成長過程が可視化できる。2.自生地での種ごとの種子寿命、埋土種子特性の解明が一挙に進む。 また、自生地播種によって親個体からの距離と発芽率の相関が検証でき、実生のセーフサイ トが明らかになる。3.自生地播種試験法を応用することにより、費用対効果の高い野生復帰が実現する。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (28 results)

All 2024 2023 2022 2021

All Journal Article (9 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 5 results) Presentation (18 results) (of which Int'l Joint Research: 6 results,  Invited: 7 results) Book (1 results)

  • [Journal Article] Symbiotic Fungi for Germination of Cypripedium japonicum (Orchidaceae) Revealed by in situ Seed-sowing2024

    • Author(s)
      Y. Yamashita, K. Fujii, Y. Ikeyama, Y. Ogura-Tsujita, T. Kurosawa, T. Yukawa
    • Journal Title

      The Proceedings of the 23rd World Orchid Conference.

      Volume: 23 Pages: 68-69

    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Journal Article] Biodiversity and Conservation of Japanese Native Orchids.2024

    • Author(s)
      T. Yukawa, C. Tsutsumi, Y. Yamashita, K. Suzuki, Y. Ogura-Tsujita.
    • Journal Title

      The Proceedings of the 23rd World Orchid Conference.

      Volume: 23 Pages: 49-50

    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Journal Article] Genetic Diversity and Structure of the Endangered Lady’s Slipper Orchid Cypripedium japonicum Thunb. (Orchidaceae) in Japan.2023

    • Author(s)
      Yamashita, Y., N. Satoh, T. Kurosawa and S. Kaneko.
    • Journal Title

      Population Ecology.

      Volume: 65(1) Issue: 1 Pages: 54-63

    • DOI

      10.1002/1438-390x.12134

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 4種の菌従属栄養植物(アオキラン、ヒメノヤガラ、シロテンマ、ホクリクムヨウラン)の新産地報告.2023

    • Author(s)
      山下由美・山下俊之.
    • Journal Title

      フロラ福島

      Volume: 35 Pages: 1-4

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] 福島県昭和村矢の原湿原北湿原の植物相と植生.2023

    • Author(s)
      高橋俊哉・山下由美・山ノ内崇志・黒沢高秀.
    • Journal Title

      福島大学地域創造

      Volume: 34(2) Pages: 135-154

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Molecular identification of seed‐feeding flies dissected from herbarium specimens clarifies the 100‐year history of parasitism by Japanagromyza tokunagai in Japan.2022

    • Author(s)
      Yamashita, Y., Ogura‐Tsujita, Y., Nagata, N., Kurosawa, T., & Yukawa, T.
    • Journal Title

      Ecological Research

      Volume: 37 Issue: 2 Pages: 240-256

    • DOI

      10.1111/1440-1703.12283

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] New host plant species of <i>Japanagromyza tokunagai</i> and its distribution including northern and southern limits in Japan2021

    • Author(s)
      辻田有紀・山下由美・村田美空・首藤光太郎・天野正晴・遊川知久.
    • Journal Title

      Japanese Journal of Entomology (New Series)

      Volume: 24 Issue: 3 Pages: 55-63

    • DOI

      10.20848/kontyu.24.3_55

    • NAID

      130008106693

    • ISSN
      1343-8794, 2432-0269
    • Year and Date
      2021-09-25
    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genetic Diversification of <i>Oberonia</i> (Orchidaceae) in Japan, with Implications for the Species Taxonomy2021

    • Author(s)
      Chie Tsutsumi, Ayako Maeda, Atsushi Abe, Yumi Yamashita, Tomohisa Yukawa and Satoshi Kakishima.
    • Journal Title

      Bulletin of the National Museum of Nature and Science. Series B, Botany

      Volume: 47 Issue: 3 Pages: 131-141

    • DOI

      10.50826/bnmnsbot.47.3_131

    • NAID

      130008081076

    • ISSN
      1881-9060, 2434-0936
    • Year and Date
      2021-08-20
    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Differing Life-History Strategies of Two Mycoheterotrophic Orchid Species Associated with Leaf Litter- and Wood-Decaying Fungi2021

    • Author(s)
      Ogura-Tsujita Yuki、Tetsuka Kenshi、Tagane Shuichiro、Kubota Miho、Anan Shuichiro、Yamashita Yumi、Tone Koichi、Yukawa Tomohisa
    • Journal Title

      Diversity

      Volume: 13 Issue: 4 Pages: 161-161

    • DOI

      10.3390/d13040161

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Symbiotic Fungi for Germination of Cypripedium japonicum (Orchidaceae) Revealed by in situ Seed-sowing2024

    • Author(s)
      Y. Yamashita, K. Fujii, Y. Ikeyama, Y. Ogura-Tsujita, T. Kurosawa, T. Yukawa
    • Organizer
      The 23rd World Orchid Conference
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ランはなぜ木に登ったか?ラン科の生活形進化に及ぼした菌類のインパクト2024

    • Author(s)
      遊川知久・蘭光健人・堤千絵・山下由美・辻田有紀
    • Organizer
      日本生態学会第71回全国大会 (2024年3月、横浜) シンポジウム
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 茨城県におけるオオウメガサソウの種子発芽に関与する菌類の分子同定2024

    • Author(s)
      伊藤彩乃・庄司顕則・大和政秀・糟谷大河・山下由美・遊川知久
    • Organizer
      日本菌学会第68回大会(八戸)
    • Related Report
      2023 Annual Research Report
  • [Presentation] 菌従属栄養植物タカツルランの菌根菌の多様性把握と経年変化2024

    • Author(s)
      堀川真子・手塚賢至・久保田美帆・阿南壮一郎・刀禰浩一・山下由美・辻田有紀
    • Organizer
      日本菌学会第68回大会(八戸)
    • Related Report
      2023 Annual Research Report
  • [Presentation] ヒメノヤガラの分類学的再検討と日本新産種 Chamaegastrodia inverta の発見2024

    • Author(s)
      佐々木晴大・山ノ内崇志・黒沢高秀・ 遊川知久・山下由美
    • Organizer
      第23回日本植物分類学会大会(仙台)
    • Related Report
      2023 Annual Research Report
  • [Presentation] Biodiversity and Conservation of Japanese Native Orchids.2024

    • Author(s)
      T. Yukawa, C. Tsutsumi, Y. Yamashita, K. Suzuki, Y. Ogura-Tsujita
    • Organizer
      The 23rd World Orchid Conference
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 野外播種試験で明らかになったクマガイソウ(ラン科)の種子発芽特性2022

    • Author(s)
      山下由美・藤井健太・池山裕一郎・藤森祥平・辻田有紀・黒沢高秀・遊川知久
    • Organizer
      東北植物学会第12回大会
    • Related Report
      2022 Research-status Report
  • [Presentation] Three epiphytic orchids harbor distinct mycorrhizal communities. even in co-existing individuals: The link to in vitro symbiotic compatibility.2022

    • Author(s)
      Kento Rammitsu, Masaru Goto, Yumi Yamashita, Tomohisa Yukawa, Yuki Ogura-Tsujita.
    • Organizer
      The 11th International Conference on Mycorrhiza (ICOM11) and the 5th International Molecular Mycorrhiza Meeting (iMMM5).
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] 野外播種試験で明らかになったクマガイソウ(ラン科)の発芽・繁殖特性.2022

    • Author(s)
      山下由美・藤井健太・池山裕一・藤森祥平・辻田有紀・黒沢高秀・遊川知久.
    • Organizer
      日本菌学会第66回大会
    • Related Report
      2022 Research-status Report
  • [Presentation] 日本のジガバチソウ(ラン科) から単離された菌根菌.2022

    • Author(s)
      堤 千絵・細矢 剛・山下 由美・遊川 知久.
    • Organizer
      日本植物学会第86回大会.
    • Related Report
      2022 Research-status Report
  • [Presentation] さく葉標本に残された寄生バエの非破壊サンプリング法開発と分子同定-ランミモグリバエによる食害の歴史を押し葉標本から探る-2022

    • Author(s)
      山下由美
    • Organizer
      九州大学比較社会文化研究院 生物多様性講座総合演習
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] シードパケットを使った野外播種試験2022

    • Author(s)
      山下由美
    • Organizer
      種子・胞子・組織培養を使った保全フォーラム-小笠原の絶滅危惧種に注目して
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] ヒメノヤガラ(ラン科ヒメノヤガラ属)集団間の花形質と繁殖特性の検討.2022

    • Author(s)
      佐々木晴大・山下由美・山ノ内崇志・黒沢高秀・遊川知久
    • Organizer
      日本植物分類学会第22回大会
    • Related Report
      2022 Research-status Report
  • [Presentation] モイワラン、葉っぱ無し生活の秘密2022

    • Author(s)
      山下由美
    • Organizer
      企画展・学芸員は見た!札幌の絶滅危惧植物展・ミュージアムトーク
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] 野外播種試験で明らかになったクマガイソウ(ラン科)の発芽・繁殖特性2022

    • Author(s)
      山下由美・藤井健太・池山裕一郎・藤森祥平・辻田有紀・黒沢高秀・遊川知久
    • Organizer
      日本植物分類学会第21回大会
    • Related Report
      2021 Research-status Report
  • [Presentation] Clonal Structure of the largest wild and ex situ conserved populations of Cypripedium japonicum Thunb. (Orchidaceae) in Japan.2021

    • Author(s)
      Yumi YAMASHITA, Natsuki SATOH, Takahide KUROSAWA, Shingo KANEKO, and Tomohisa YUKAWA
    • Organizer
      23rd World Orchid Congress
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] 緑色菌従属栄養植物カイサカネランの色素体構造.2021

    • Author(s)
      山下由美・高木大輔・池田健一・末次健司・黒沢高秀・遊川知久.
    • Organizer
      東北植物学会第11回大会シンポジウム
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] Molecular identification of seed-feeding flies dissected from herbarium specimens clarifies the 100-year history of parasitism by Japanagromyza tokunagai in Japan.2021

    • Author(s)
      Yumi Yamashita, Yuki Ogura-Tsujita, Nobuaki Nagata, Takahide Kurosawa, Tomohisa Yukawa.
    • Organizer
      The 9th East Asian Plant Diversity and Conservation Virtual Symposium 2021
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Book] 福島スミレ図鑑2022

    • Author(s)
      山下俊之・山下由美・遠藤雄一
    • Total Pages
      121
    • Publisher
      歴史春秋社
    • Related Report
      2022 Research-status Report

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Published: 2021-04-28   Modified: 2025-01-30  

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