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Establishment of Experimental Infection of Marine Philometrid Nematodes Using Surrogate Copepod Intermediate Host

Research Project

Project/Area Number 21K05735
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 40030:Aquatic bioproduction science-related
Research InstitutionMeguro Parasitological Museum

Principal Investigator

Ogawa Kazuo  公益財団法人目黒寄生虫館, その他部局等, 名誉館長 (20092174)

Co-Investigator(Kenkyū-buntansha) 白樫 正  近畿大学, 水産研究所, 准教授 (70565936)
Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2023: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsブリ筋肉線虫 / 代理宿主 / シオダマリミジンコ / 生活環 / フィロメトラ類 / フィロメトロイデス / フィロメトラ科線虫 / 中間宿主
Outline of Research at the Start

海産魚に寄生するフィロメトラ科線虫は宿主魚の商品価値を喪失させ、水産業上問題となる。フィロメトラ類はカイアシ類を中間宿主、魚類を終宿主とする生活環を持つ。10種以上が知られているが、中間宿主は不明である。そこで、本来の中間宿主ではないが、飼育が容易で、実験的に種々の線虫の中間宿主になることが報告されているシオダマリミジンコを代理中間宿主として用いて、線虫のふ化幼生を感染させ、さらにそれを魚類終宿主に投して生活環を完結させる。このことによって、天然の生活環が不明であっても、終宿主体内の幼生の移動、成長、成熟、生殖生態や感染サイクルの季節性などの基礎的知見を得ることができる。

Outline of Final Research Achievements

We used the copepod Tigriopus japonicus as a surrogate host to experimentally complete the life cycle of Philometoides seriolae, a nematode infecting the skeletal muscle of Japanese amberjack Seriola quinqueradiata. P. seriolae developed to the third stage larvae which are infective to the final host, within the copepod fed with freshly hatched larvae. Eleven months after given infected copepods, two out of seven amberjacks were found infected with a mature female nematode in the skeletal muscle. It would take more than a year for P. seriolae females to produce and disseminate larvae, as those recovered from the experimental fish only contained developing eggs in the uterus. Most larvae retrieved from fully mature females had an exuvium, suggesting that they were already at the second stage. In addition, we found a hitherto undescribed male worm in the skeletal muscle of wild Japanese amberjack.

Academic Significance and Societal Importance of the Research Achievements

ブリ筋肉線虫は宿主のブリに対して強い害作用は示さず、人体寄生虫でもない。しかし体側筋に寄生している雌成虫は最大体長が50 cmにも達する大型寄生虫のため、目につきやすく、ブリの商品価値を落とす水産上重要な寄生虫である。一方、本線虫の生物学的知見はきわめて乏しく、生活環も解明されていない。本研究によってシオダマリミジンコを代理の中間宿主とすることで実験的にふ化幼生を成虫にまで発育させることに成功し、本線虫の生活環が回るのに少なくとも1年を要することが明らかになった。この実験系は本線虫のみならず、他のフィロメトラ科線虫の寄生生態解明の研究に有用な手段といえる

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (9 results)

All 2024 2023 2022

All Journal Article (5 results) (of which Peer Reviewed: 4 results) Presentation (4 results)

  • [Journal Article] Infections of Philometra madai (Nematoda: Philometridae) in the gonads of a high-growth broodstock population of red seabream Pagrus major2024

    • Author(s)
      Yuki Tomita, Keitaro Kato, Youhei Washio, Sho Shirakashi
    • Journal Title

      Fish Pathology

      Volume: 59

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] <i>Microsporidium seriolae</i> and Probable <i>Philometroides seriolae</i> Infection in Yellowtail Amberjack <i>Seriola aureovittata</i>2023

    • Author(s)
      Yanagi Soetsu、Imaoka Yoshiaki、Ogawa Kazuo
    • Journal Title

      Fish Pathology

      Volume: 58 Issue: 4 Pages: 180-183

    • DOI

      10.3147/jsfp.58.180

    • ISSN
      0388-788X, 1881-7335
    • Year and Date
      2023-12-15
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Parasites in the Skeletal Muscle of Amberjacks <i>Seriola</i> spp. in Japan: A Review2023

    • Author(s)
      Ogawa Kazuo
    • Journal Title

      Fish Pathology

      Volume: 58 Issue: 4 Pages: 127-145

    • DOI

      10.3147/jsfp.58.127

    • ISSN
      0388-788X, 1881-7335
    • Year and Date
      2023-12-15
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] <i>Philometroides seriolae</i> Recovered from the Swimbladder of Japanese Amberjack <i>Seriola quinqueradiata</i>2023

    • Author(s)
      Ogawa Kazuo、Abe Masaaki、Hara Satoko、Ueno Mutsuki、Shirakashi Sho
    • Journal Title

      Fish Pathology

      Volume: 58 Issue: 2 Pages: 71-73

    • DOI

      10.3147/jsfp.58.71

    • ISSN
      0388-788X, 1881-7335
    • Year and Date
      2023-06-15
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of experimental life cycle of Philometroides seriolae (Nematoda: Philometridae) using surrogate copepod intermediate host.2023

    • Author(s)
      Ogawa, K., N. Sata, Y. Sugihara, H. Miyazaki, M. Ueno, S. Kuramochi. and S. Shirakashi
    • Journal Title

      Fish Pathology

      Volume: 58

    • Related Report
      2022 Research-status Report
  • [Presentation] シオダマリミジンコを代理宿主とした海産フィロメトラ科線虫 3 種の実験感染2023

    • Author(s)
      上野睦生・白樫 正・佐田直也・小川和夫
    • Organizer
      日本魚病学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] マダイ親魚における生殖腺線虫の寄生実態2023

    • Author(s)
      冨田雄貴・白樫 正・家戸敬太郎・鷲尾洋平
    • Organizer
      日本魚病学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] ブリ筋肉線虫の生活環に関する研究:ブリへの感染実験の成立2022

    • Author(s)
      小川和夫・佐田直也・杉原志貴・宮﨑悠暉・白樫 正・上野睦生・倉持奏良・山本 剛
    • Organizer
      令和 4 年度日本魚病学会秋季大会
    • Related Report
      2022 Research-status Report
  • [Presentation] ブリ筋肉線虫の生活環に関する研究:雄成虫発見と代理宿主内の発育2022

    • Author(s)
      小川和夫・佐田直也・杉原志貴・白樫 正・上野睦生・倉持奏良
    • Organizer
      令和 4 年度日本魚病学会春季大会
    • Related Report
      2021 Research-status Report

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Published: 2021-04-28   Modified: 2025-01-30  

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