| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2023: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2022: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
In this study, upstream and downstream studies were conducted to elucidate the fucoxanthin biosynthesis pathway in brown algae. In the analysis of the upstream pathway, we found genes homologous to known plant carotenoid biosynthesis genes, PSY1,2, PDS1, ZISO1, ZDS1, CRTISO1,2, LCYb1, CYP97B1,2, ZEP1,2. For the downstream pathway, we searched for genes that might be involved in the biosynthetic reaction, but could not find any candidate genes. Functional analysis of a gene homologous to VDL1 in diatoms was also performed, but activity could not be detected. However, in the course of this analysis, it became possible for the first time to produce neoxanthin in E. coli.
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