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Identification of female-attractive substance secreted from male kidney in nest-spawning teleosts

Research Project

Project/Area Number 21K05780
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 40040:Aquatic life science-related
Research InstitutionGifu University

Principal Investigator

Koya Yasunori  岐阜大学, 教育学部, 教授 (30273113)

Co-Investigator(Kenkyū-buntansha) 山家 秀信  東京農業大学, 生物産業学部, 准教授 (40423743)
Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2023: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2021: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords性フェロモン / 営巣繁殖 / 腎臓 / 雌誘引 / 多糖類 / トゲウオ科 / ハゼ科 / スピギン / 尿 / 排卵 / 精子形成 / トミヨ属 / 誘引 / 営巣 / 輸尿管 / カワヨシノボリ / フェロモン / 硬骨魚類
Outline of Research at the Start

雄が繁殖のための巣を作る魚(営巣繁殖魚)では、雄は自分の巣やそこに産み付けられた卵を守るため、巣から離れることができない。従って、雄が自分の子孫をたくさん残すためには、産卵可能な雌に巣まで来てもらう必要がある。すなわち、巣にいる雄は雌を化学的に誘引する性フェロモンを放出していると考えられる。これまでの研究からトゲウオ類とカジカ類では雄の腎臓で作られた物質が尿ととともに体外に放出されて雌を誘引している可能性が示されている。本研究では、この点を他の営巣繁殖魚も含めて明らかにするとともに、雌を誘引する成分がどのような物質であるのかを分析機器を用いて明らかにする。

Outline of Final Research Achievements

In male Rhinogobius, kidneys were enlarged during the breeding season and the production of polysaccharide-containing components (molecular weights 74 and 33 kDa) in the renal tubule epithelium is stimulated. Kidney extracts from males were shown to contain components that attract ovulating females.
During the breeding season, male Pungitius enlarged his kidney, and activated the production of spiggin, a substance that adheres plant fragments, in the renal tubular epithelium. Kidney extracts from breeding males contain a substance that attracts ovulated females, with a molecular weight of 3 kDa or more. Nests built by males were shown to contain a substance that attracts females, strongly suggesting that spiggin is a female attractant. It was thought that spiggin production in the kidney is stimulated by androgen.

Academic Significance and Societal Importance of the Research Achievements

本研究および先行研究での知見から、営巣繁殖する魚類には雄が腎臓で産生する物質によって雌を巣まで誘引するという普遍性が存在することを示すことができた。これは営巣繁殖魚において、繁殖巣を防衛しつつ繁殖成功を最大にする上で理にかなった仕組みであり、今後も多くの営巣繁殖魚の繁殖生理生態を理解する上で基礎となる概念になり得る。
トゲウオ科魚類に見られる特異な造巣生態に関しては、巣材を接着する成分が雌誘引物質に由来する可能性を示し、単純な縄張り繁殖から、営巣繁殖、さらには造巣繁殖へと進化する過程で、雌誘引物質が巣材接着物質へと機能を進化させてきたと言う新たな概念を示すことができた。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (25 results)

All 2024 2023 2022 2021

All Journal Article (11 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 11 results,  Open Access: 2 results) Presentation (14 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Process of gametogenesis in golden venus chub, Hemigrammocypris neglecta (Cyprinidae), with special regard to the comparison in initiation pattern of gametogenesis with other cyprinids2024

    • Author(s)
      Koya Yasunori、Morishita Ryotaro、Ito Gen
    • Journal Title

      Ichthyological Research

      Volume: n/a~ Issue: 3 Pages: 410-421

    • DOI

      10.1007/s10228-024-00949-1

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sperm allocation in relation to male?male aggression and courtship in an externally fertilizing fish, the medaka2023

    • Author(s)
      Kondo Yuki、Kohda Masanori、Koya Yasunori、Awata Satoshi
    • Journal Title

      Animal Behaviour

      Volume: 202 Pages: 9-19

    • DOI

      10.1016/j.anbehav.2023.05.011

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rhamphocottus nagaakii (Cottoidea: Rhamphocottidae), a new species of grunt sculpin from the Northwestern Pacific, with notes on the phylogeography of the genus Rhamphocottus.2023

    • Author(s)
      Munehara H., Togashi K, Yamada S, Higashimura T, Yamazaki A, Suzuki S, Abe T, Awata S, Koya Y, Tsuruoka O.
    • Journal Title

      Icthyological Research

      Volume: 70 Pages: 268-285

    • DOI

      10.1007/s10228-022-00885-y

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Phylogeographic structure of an endemic lineage of the eight-barbel loach Lefua echigonia around the Suzuka and Yoro Mountains, central Honshu, Japan2022

    • Author(s)
      Gen Ito, Yasunori Koya
    • Journal Title

      Biogeography

      Volume: 24 Issue: 0 Pages: 39-45

    • DOI

      10.11358/biogeo.24.39

    • ISSN
      1345-0662, 1880-8085
    • Year and Date
      2022-09-20
    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Records and genetic characteristics of the bitterling <i>Tanakia lanceolata</i> established in the Naruse River system, Miyagi Prefecture, Japan2022

    • Author(s)
      伊藤 玄、旗 薫、北村 淳一、古屋 康則
    • Journal Title

      Japanese Journal of Ichthyology

      Volume: 69 Issue: 1 Pages: 57-62

    • DOI

      10.11369/jji.20-24

    • NAID

      130008101511

    • ISSN
      0021-5090, 1884-7374
    • Year and Date
      2022-04-25
    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] 大阪府淀川水系における国内外来ミナミアカヒレタビラの確認と遺伝的特徴2022

    • Author(s)
      伊藤玄・小山直人・川瀬成吾・古屋康則
    • Journal Title

      地域自然史と保全

      Volume: 44 Pages: 45-50

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Fertilization modes and the evolution of sperm characteristics in marine fishes: Paired comparisons of externally and internally fertilizing species.2022

    • Author(s)
      Takeshi I., Morita M., Okuno S., Inaba H., Shiba K., Munehara H., Koya Y., Homma M., Awata S.
    • Journal Title

      Ecology and Evolution

      Volume: 12 Issue: 12 Pages: 1-8

    • DOI

      10.1002/ece3.9562

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Records and genetic characteristics of exotic bitterling, <i>Acleilognathus tabira</i> subspp., from northern Mie Prefecture, Japan2021

    • Author(s)
      伊藤 玄、北村 淳一、野口 亮太、長太 伸章、古屋 康則
    • Journal Title

      Japanese Journal of Ichthyology

      Volume: 68 Issue: 1 Pages: 47-52

    • DOI

      10.11369/jji.20-034

    • NAID

      130008036499

    • ISSN
      0021-5090, 1884-7374
    • Year and Date
      2021-04-25
    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] The first record of egg masses in tunicates deposited by the snubnose sculpin, Orthonopias triacis, from the Northeastern Pacific : evidence for convergent evolution of an unusual reproductive strategy2021

    • Author(s)
      Awata Satoshi、Ito Takeshi、Crow Karen D.、Koya Yasunori、Munehara Hiroyuki
    • Journal Title

      Journal of Fish Biology

      Volume: 100 Issue: 1 Pages: 82-91

    • DOI

      10.1111/jfb.14920

    • URL

      https://ocu-omu.repo.nii.ac.jp/records/2019970

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 三重県加茂川水系におけるやりタナゴ<i>Tanakia lanceolata</i>の初記録2021

    • Author(s)
      久保敦暉、伊藤 玄、野口亮太、北村淳一、古屋康則
    • Journal Title

      生物地理学会会報

      Volume: 76 Pages: 70-74

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Chemical cues for intraspecific chemical communication and interspecific interactions in aquatic environments: applications for fisheries and aquaculture2021

    • Author(s)
      Kamio Michiya、Yambe Hidenobu、Fusetani Nobuhiro
    • Journal Title

      Fisheries Science

      Volume: 88 Issue: 2 Pages: 203-239

    • DOI

      10.1007/s12562-021-01563-0

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Presentation] Does nest-building glue secreted by the kidney of male stickleback attract females?2023

    • Author(s)
      Miki NAGAYA; Hidenobu YASMBE; Yasunori KOYA
    • Organizer
      11th Indo-Pacific Fish Conference and Annual Conference of the Australian Society for Fish Biology
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Males of nest-spawn fish lure females using attractants produced by their kidney2023

    • Author(s)
      Yasunori KOYA; Miki NAGAYA
    • Organizer
      11th Indo-Pacific Fish Conference and Annual Conference of the Australian Society for Fish Biology
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 営巣しているカワヨシノボリの雄は雌を巣まで化学的に誘引するか?2023

    • Author(s)
      長屋美希・中西陽人・舟崎大翔・古屋康則
    • Organizer
      第57回日本魚類学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] ゲウオ科のオスが分泌する巣材接着物質はメスを誘引するか?2023

    • Author(s)
      長屋美希・山家秀信・古屋康則
    • Organizer
      令和5年度日本動物学会中部支部大会(三重大会)
    • Related Report
      2023 Annual Research Report
  • [Presentation] 繁殖期のカワヨシノボリの雄は腎臓で産生される物質で排卵雌を誘引する2023

    • Author(s)
      舟﨑大翔・長屋美希・古屋康則
    • Organizer
      令和5年度日本動物学会中部支部大会(三重大会)
    • Related Report
      2023 Annual Research Report
  • [Presentation] トゲウオ科の特異な精子形成過程2023

    • Author(s)
      長屋美希・山家秀信・古屋康則
    • Organizer
      令和5年度日本水産学会春季大会
    • Related Report
      2022 Research-status Report
  • [Presentation] 鈴鹿山脈の低標高地帯に着目した揖斐川・琵琶湖水系間における河川争奪の検証2022

    • Author(s)
      伊藤玄・古屋康則
    • Organizer
      第56回日本魚類学会年会
    • Related Report
      2022 Research-status Report
  • [Presentation] ミナミメダカの雌は雄の配偶行動を誘発するフェロモンを放出しているのか?2022

    • Author(s)
      近藤湧生・児玉恵弥・安房田智司・古屋康則
    • Organizer
      第56回日本魚類学会年会
    • Related Report
      2022 Research-status Report
  • [Presentation] トミヨ類の雄の腎臓から分泌・放出される巣材接着成分による雌の誘引性について2022

    • Author(s)
      長屋美希・山家秀信・古屋康則
    • Organizer
      第56回日本魚類学会年会
    • Related Report
      2022 Research-status Report
  • [Presentation] ミナミメダカの繁殖で同時放精スニーキングを行う雄の行動の特徴2022

    • Author(s)
      若園彩花・古屋康則
    • Organizer
      第56回日本魚類学会年会
    • Related Report
      2022 Research-status Report
  • [Presentation] シロヒレタビラの遺伝的集団構造2021

    • Author(s)
      伊藤玄、小山直人、野口亮太、田畑諒一、川瀬成吾、北村淳一、古屋康則
    • Organizer
      日本魚類学会
    • Related Report
      2021 Research-status Report
  • [Presentation] アブラボテの雌の産卵生態-産卵周期に着目して2021

    • Author(s)
      草留大岳、古屋康則
    • Organizer
      日本魚類学会
    • Related Report
      2021 Research-status Report
  • [Presentation] 耳石Sr/Ca比分析によるクルメサヨリの回遊履歴推定2021

    • Author(s)
      阿部勝徳、鬼倉徳雄、加納光樹、桑原正樹、古屋康則、杉山秀樹、東信行、曽我部篤
    • Organizer
      日本魚類学会
    • Related Report
      2021 Research-status Report
  • [Presentation] ミナミメダカにおける雄間の優劣と交配をめぐる立場の関係2021

    • Author(s)
      若園 彩花、古屋 康則
    • Organizer
      日本動物学会
    • Related Report
      2021 Research-status Report

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Published: 2021-04-28   Modified: 2025-01-30  

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