| Project/Area Number |
21K05847
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 41040:Agricultural environmental engineering and agricultural information engineering-related
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| Research Institution | Gifu University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2023: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | Circadian rthytm / Fresh produce / Quality control / Circadian rhythm / Quality / Molecular timetable |
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| Outline of Research at the Start |
Circadian clock (CC) system produces a circadian rhythm (CR) of about 24 h a day. However, the mechanism of CR in fresh produce is not clear and the detection tool of CR is not available. This study aims to propose the innovative method for CR state detection in fresh produce from CC gene expression with the following procedures;
1) Expression of CC genes of fresh produce stored under various effective storage conditions
2) Detection of CR state (body time) by molecular timetable method (MTM)
3) Validating the probability of using MTM and predictive model for quality control during storage.
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| Outline of Final Research Achievements |
The molecular timetable of soybean sprouts was proposed. The effect of temperature and CA storage on the clock genes and quality attributes and their related genes were examined. By fitting the gene expression level with the cosine curve equation, it was successfully found that the circadian rhythm existed under constant dark storage conditions of soybean sprouts. A significant rhythm in clock gene expression was observed in control. In contrast, low temperature storage diminished the cyclic expression of clock gene expression. Additionally, high CO2 concentrations during storage disturbed the circadian clock by affecting the phase and amplitude of each gene; for low O2, it was only affected by amplitude. Interestingly, low temperature, low O2, and high CO2 maintained postharvest quality, including reduced respiration, weight loss and browning incidence. The expression behaviors of postharvest quality attribute-related genes were also influenced by the storage treatments.
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| Academic Significance and Societal Importance of the Research Achievements |
The link between clock disruption and quality maintenance of soybean sprouts was first reported. The knowledge about the existence of the circadian clock and its response behavior during storage will help to develop a new postharvest preservation technology by controlling circadian rhythm.
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