• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Elucidation of cancer resistance mechanisms in elephants

Research Project

Project/Area Number 21K05960
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 42020:Veterinary medical science-related
Research InstitutionKitasato University

Principal Investigator

Orino Koichi  北里大学, 獣医学部, 教授 (60214235)

Co-Investigator(Kenkyū-buntansha) 吉川 泰永  北里大学, 獣医学部, 准教授 (00552043)
Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2023: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2022: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywordsゾウ / 発癌防御機構 / FANCL / FANCB / Fanconi/BRCA経路 / DNA損傷修復 / Fanconi anemia / 繊維芽細胞 / FANCC / 腫瘍化 / ファンコニ貧血
Outline of Research at the Start

癌になりにくい動物種として、ハダカデバネズミ、メクラネズミ、ゾウ、クジラなどが報告されているが、本研究では細胞の癌化防御機構が不明なゾウに注目して研究を行い、ゾウの発癌防御機構を解明し、この知識を最終的に獣医療や医療へ応用することを目的とした。すなわち、本研究計画では①正常なゾウ繊維芽細胞を癌化させて、元の正常な線維芽細胞と比較することで、ゾウの癌化に必要な遺伝子、タンパク質を特定すること、および、②ゾウ独自に進化したと考えられる染色体安定性機構の中で、最も進化速度が速い遺伝子であるDNA損傷修復タンパク質FANCLとFANCLが関わるDNA損傷修復経路のFanconi/BRCA経路の機能解析を行う。

Outline of Final Research Achievements

Some animals, such as the naked mole rats, blind mole rats, elephants and the whales have been reported as cancer resistant animals. In this study, we focused on the elephants, whose cellular resistant mechanisms against tumorigenesis are unknown, with the aim of elucidating its mechanisms. This research aims to (1) attempt to generate immortalized cells by expressing the T antigen of african elephant polyomavirus in normal elephant fibroblasts, and (2) investigate the one of DNA damage repair protein FANCL, the most acerated region in the elephants’ genome, and the Fanconi/BRCA DNA damage repair pathway, in which FANCL is involved.

Academic Significance and Societal Importance of the Research Achievements

癌研究は、培養細胞ならヒトやマウスの細胞株、実験動物ならマウスを使った研究が盛んに行われてきた。これらの研究は、癌がどのように発症するのかを解明することに多大な貢献をしてきた。近年、分子細胞学的手技の発展により、一般的なモデル生物ではない癌になりにくい動物種が発癌防御機構を解析するのに有用であることが示唆されるようになってきた。癌になりにくい動物種として、ハダカデバネズミ、メクラネズミ、ゾウ、クジラなどが報告されているが、本研究では細胞の癌化防御機構が不明なゾウに注目して研究を行い、ゾウの発癌防御機構を解明し、この知識を最終的に獣医療や医療へ応用することを目的とした。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (11 results)

All 2023 Other

All Int'l Joint Research (2 results) Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (6 results)

  • [Int'l Joint Research] Fox Chase Cancer Center(米国)

    • Related Report
      2022 Research-status Report
  • [Int'l Joint Research] Fox Chase Cancer Center(米国)

    • Related Report
      2021 Research-status Report
  • [Journal Article] Phosphorylation by ATR triggers FANCD2 chromatin loading and activates the Fanconi anemia pathway2023

    • Author(s)
      Kupculak Marian、Bai Fengxiang、Luo Qiang、Yoshikawa Yasunaga、Lopez-Martinez David、Xu Hannan、Uphoff Stephan、Cohn Martin A.
    • Journal Title

      Cell Reports

      Volume: 42 Issue: 7 Pages: 112721-112721

    • DOI

      10.1016/j.celrep.2023.112721

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Formation of free oocysts in <i>Anopheles</i> mosquitoes injected with <i>Plasmodium</i> ookinetes2023

    • Author(s)
      HARAGUCHI Asako、TAKANO Makoto、HAKOZAKI Jun、NAKAYAMA Kazuhiko、NAKAMURA Sakure、YOSHIKAWA Yasunaga、FUKUMOTO Shinya、KUSAKISAKO Kodai、IKADAI Hiromi
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 85 Issue: 9 Pages: 921-928

    • DOI

      10.1292/jvms.23-0099

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comparisons of beta2-microglobulin, apolipoprotein A1, and immunoglobulins (IgG and IgM) detected in the serum and urine from individual cats2023

    • Author(s)
      Katagai Michio、Imatomi Haruka、Tshuchida Yashuhiko、Katayama Kinya、Yoshikawa Yasunaga、Orino Koichi
    • Journal Title

      Austral Journal of Veterinary Sciences

      Volume: 55 Issue: 3 Pages: 197-202

    • DOI

      10.4206/ajvs.553.06

    • Related Report
      2023 Annual Research Report
  • [Presentation] Highly conserved region in BRCA2 suppresses the RAD51-interaction activity of BRC repeats2023

    • Author(s)
      Zida Zhu, Taisuke Kitano, Masami Morimatsu, Kazuhiko Ochiai, Toshina Ishiguro-Oonuma, Xianghui Lin, Koichi Orino, Yasunaga Yoshikawa
    • Organizer
      Eukaryotic DNA Replication & Genome Maintenance
    • Related Report
      2023 Annual Research Report
  • [Presentation] Reduced translation efficiency due to novel splicing variants in 5′ untranslated region and identification of novel cis-regulatory elements in canine and human BRCA22023

    • Author(s)
      Yasunaga Yoshikawa, Hajime Kozuma, Chihiro Mochizuki, Zida Zhu, Masami Morimatsu, Koichi Orino
    • Organizer
      Eukaryotic DNA Replication & Genome Maintenance
    • Related Report
      2023 Annual Research Report
  • [Presentation] ゾウFANCL-FANCB間の相互作用はヒトホモログより強い2023

    • Author(s)
      日置七海, 北野泰佑, 朱子達, 武田一貴, 折野宏一, 吉川泰永
    • Organizer
      第166回日本獣医学会学術集会講演要旨集
    • Related Report
      2023 Annual Research Report
  • [Presentation] がん抑制遺伝子BRCA2の新規転写調節機構の解明2023

    • Author(s)
      望月千尋, 上妻創, 朱子達, 北野泰佑, 折野宏一, 森松正美, 吉川泰永
    • Organizer
      第166回日本獣医学会学術集会講演要旨集
    • Related Report
      2023 Annual Research Report
  • [Presentation] ハマダラカのマラリア原虫感染に対する新たな応答分子の探索2023

    • Author(s)
      原口麻子, 高野真, 箱崎純, 中山和彦, 中村咲蓮, 吉川泰永, 福本晋也, 草木迫浩大, 筏井宏実
    • Organizer
      第46回日本分子生物学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] がん抑制遺伝子BRCA2の5’非翻訳領域と新規シスエレメントによる発現調節2023

    • Author(s)
      吉川泰永, 上妻創, 望月千尋, ZHU Zida, 北野泰佑, 森松正美, 折野宏一
    • Organizer
      第46回日本分子生物学会年会
    • Related Report
      2023 Annual Research Report

URL: 

Published: 2021-04-28   Modified: 2025-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi