• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Functional characterization of Myc-depedent ribosomes by Ribo Mega-SEC coupled to mass spectrometry-based proteomics

Research Project

Project/Area Number 21K06138
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 43060:System genome science-related
Research InstitutionThe University of Tokushima

Principal Investigator

YOSHIKAWA Harunori  徳島大学, 先端酵素学研究所, 助教 (60709567)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2023: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2022: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords特殊化リボソーム / サイズ排除クロマトグラフィー / プロテオミクス / リボソーム / 翻訳 / がん / プロテオーム解析 / Myc
Outline of Research at the Start

およそ7割の腫瘍で発現量が増加している転写因子Mycは、細胞内タンパク質合成装置であるリボソームの構成成分とそのリボソームの合成に必須な因子群の発現量を増加させることが知られている。しかし産生されたリボソームの質的変化とその翻訳能の変化については十分な知見が得られていない。本研究では、申請者独自のリボソームの分離法「Ribo Mega-SEC」とプロテオミクス技術の融合による簡便なリボソームのインタラクトーム解析法を活用して、Myc依存的なリボソームとの相互作用タンパク質の変化を系統的・網羅的に解析する。

Outline of Final Research Achievements

The ribosome is an intracellular machinery to translate mRNAs to proteins. In cancer cells, ribosome biogenesis which occurs in nucleoli is highly activated to increase in the ribosomes, leading to elevated protein synthesis enabling cell growth and proliferation. Myc, a transcription factor, increases in the ribosomes by upregulating transcription of ribosomal protein genes and ribosomal RNA. In this study, we hypothesized that Myc can alter ribosome biogenesis and generate Myc-dependent specialised ribosomes, resulting in changes in mRNA translation leading to tumorigenesis. Therefore, I aimed to identify changes in protein composition in the ribosomes between normal cells and high Myc-expressing cells.

Academic Significance and Societal Importance of the Research Achievements

細菌感染症の治癒などに大きく貢献してきた抗生物質は、現代の医療においてはなくてはならないものである。その抗生物質のほとんどはリボソームによるタンパク質合成を阻害することで細菌の増殖を抑制する。つまりリボソームの機能阻害は有用な薬剤標的の一つである。Mycが高発現している腫瘍は全体の約7割に及ぶ。本研究で明らかにされたMyc発現依存的かつ特異的リボソームはがん細胞特異的な翻訳メカニズムについての理解を深め、Mycを高発現する腫瘍において新しい治療戦略の構築にも展開できると期待される。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (13 results)

All 2024 2023 2022 Other

All Int'l Joint Research (2 results) Journal Article (5 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 5 results,  Open Access: 3 results) Presentation (5 results) (of which Invited: 3 results) Book (1 results)

  • [Int'l Joint Research] University of Dundee/University of York/Queen Mary University of London(英国)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] The Blizard Institute/School of Medicine and Dentistry/Queen Mary University of London(英国)

    • Related Report
      2021 Research-status Report
  • [Journal Article] Proteomic and functional comparison between human induced and embryonic stem cells2024

    • Author(s)
      Brenes Alejandro J.、Griesser Eva、Sinclair Linda V.、Davidson Lindsay、Prescott Alan R.、Singh Francois、Hogg Elizabeth K.J.、Espejo-Serrano Carmen、Jiang Hao、Yoshikawa Harunori、Platani Melpomeni、Swedlow Jason、Findlay Greg M.、Cantrell Doreen A.、Lamond Angus I.
    • Journal Title

      eLife

      Volume: -

    • DOI

      10.7554/elife.92025.1

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] An allele-selective inter-chromosomal protein bridge supports monogenic antigen expression in the African trypanosome2023

    • Author(s)
      Faria Joana R. C.、Tinti Michele、Marques Catarina A.、Zoltner Martin、Yoshikawa Harunori、Field Mark C.、Horn David
    • Journal Title

      Nature Communications

      Volume: 14; 8200 Issue: 1

    • DOI

      10.1038/s41467-023-44043-y

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Optimized Workflow for Enrichment and Identification of Biotinylated Peptides Using Tamavidin 2-REV for BioID and Cell Surface Proteomics2022

    • Author(s)
      Nishino Kohei、Yoshikawa Harunori、Motani Kou、Kosako Hidetaka
    • Journal Title

      Journal of Proteome Research

      Volume: 21 Issue: 9 Pages: 2094-2103

    • DOI

      10.1021/acs.jproteome.2c00130

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification and validation of new ERK substrates by phosphoproteomic technologies including Phos-tag SDS-PAGE2022

    • Author(s)
      Yoshikawa Harunori、Nishino Kohei、Kosako Hidetaka
    • Journal Title

      Journal of Proteomics

      Volume: 258 Pages: 104543-104543

    • DOI

      10.1016/j.jprot.2022.104543

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Genetic variation at mouse and human ribosomal DNA influences associated epigenetic states2022

    • Author(s)
      Rodriguez-Algarra Francisco、Seaborne Robert A. E.、Danson Amy F.、Yildizoglu Selin、Yoshikawa Harunori、Law Pui Pik、Ahmad Zakaryya、Maudsley Victoria A.、Brew Ama、Holmes Nadine、Ochoa Mateus、Hodgkinson Alan、Marzi Sarah J.、Pradeepa Madapura M.、Loose Matthew、Holland Michelle L.、Rakyan Vardhman K.
    • Journal Title

      Genome Biology

      Volume: 23 Issue: 1 Pages: 54-54

    • DOI

      10.1186/s13059-022-02617-x

    • NAID

      120007193750

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Co-Fractionation MSによる細胞内巨大タンパク質複合体の解析2023

    • Author(s)
      吉川治孝
    • Organizer
      第71回質量分析総合討論会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] Co-Fractionation MS(CF-MS)による細胞内巨大タンパク質複合体の解析2023

    • Author(s)
      吉川治孝
    • Organizer
      第23回日本蛋白質科学会年会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] 定量プロテオミクスによる核小体リボソーム生合成過程の解明2023

    • Author(s)
      吉川治孝
    • Organizer
      日本プロテオーム学会2023年大会 JPrOS2023 (21st JHUPO)
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] Protein Correlation Profilingによる細胞内巨大タンパク質複合体の解析2022

    • Author(s)
      吉川治孝
    • Organizer
      日本プロテオーム学会2022年大会
    • Related Report
      2022 Research-status Report
  • [Presentation] 核小体ヒトプレリボソームの新規分離法が切り拓く リボソーム合成因子の網羅的解析2022

    • Author(s)
      吉川治孝
    • Organizer
      第45回日本分子生物学会年会
    • Related Report
      2022 Research-status Report
  • [Book] 決定版質量分析活用スタンダード 発展編2 co-fractionation MS 新たな網羅的タンパク質複合体解析法2023

    • Author(s)
      馬場健史、松本雅記、松田史生、山本敦史、吉川治孝、及川彰、三浦大典、谷水雅治、 松井誠一、小林 大樹、増田豪、川島祐介、幡野敦、大槻純男、津曲和哉、小形公亮、石濱泰、今見考志、足達俊吾、足立 淳、...
    • Total Pages
      363
    • Publisher
      羊土社
    • Related Report
      2023 Annual Research Report

URL: 

Published: 2021-04-28   Modified: 2025-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi