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Analysis of the Unique Photoprotective Mechanism of Green Algae Mesostigma - From Freshwater Green Algae to Land Plants

Research Project

Project/Area Number 21K06224
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionHokkaido University

Principal Investigator

Takabayashi Atsushi  北海道大学, 低温科学研究所, 助教 (90546417)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2023: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords光合成 / 陸上植物への進化 / 光防御機構 / 熱放散機構 / PsbS / 緑藻 / 光化学系 / 光化学系の分離 / Native-PAGE / メソスティグマ
Outline of Research at the Start

陸上植物の誕生は地球の生命史における重要なイベントであり、淡水性緑藻であるストレプト藻の1系統が陸上植物に進化したと考えられている。そして、水中に生息する緑藻から陸上植物への進化においては光防御機構の獲得が極めて重要なプロセスであった。光防御機構の中で最も重要な機構は「熱放散機構」である。この機構は厳密に制御されており、吸収した光エネルギーが光合成活性よりも過剰な時には強く誘導される。本研究では、ストレプト藻のメソスティグマ(Mesostigma viride)の光防御機構、とりわけ熱放散機構を明らかにし、その陸上化におけるダイナミックな変化を明らかにすることを目的とする

Outline of Final Research Achievements

For plants, land is a harsh environment where they are exposed to environmental stresses such as drought, UV, and intense light. Therefore, it was necessary for plants to acquire photoprotective mechanisms as they evolved from freshwater green algae to land plants. It has been reported that the function and role of PsbS differs between green algae and land plants, but it is unclear at what point this changed. In this study, we introduced the PsbS genes from some streptophytes, the ancestors of land plants, into Arabidopsis thaliana. We found that its PsbS functions similarly to that of land plants. Interestingly, the stability of PsbS from green algae was remarkably low, suggesting that increased stability of PsbS may have been the key to their evolution into land plants.

Academic Significance and Societal Importance of the Research Achievements

植物は光に対する防御機構を持たなければ、強い太陽光を受けると直ちに枯死してしまう。
そのため、水中に生息する緑藻から陸上植物への進化においては光防御機構の獲得が極めて重要なプロセスであった。本研究ではその光防御機構の制御を司るPsbSタンパク質に着目し、陸上植物への進化においてはその安定性の向上が鍵であっただろうことを見出した。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (10 results)

All 2023 2022 Other

All Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (4 results) (of which Invited: 1 results) Remarks (2 results)

  • [Journal Article] Letter to the Editor: Weak-Acidic Clear-Native Polyacrylamide Gel Electrophoresis for the Separation of the Intact Forms of Thylakoid Protein Complexes2022

    • Author(s)
      Matsumae Renon、Kameo Shinsa、Tanaka Ryouichi、Takabayashi Atsushi
    • Journal Title

      Plant and Cell Physiology

      Volume: 63 Issue: 7 Pages: 883-885

    • DOI

      10.1093/pcp/pcac070

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Reversible down-regulation of photosystems I and II leads to fast photosynthesis recovery after long-term drought in Jatropha curcas2022

    • Author(s)
      Sapeta Helena、Yokono Makio、Takabayashi Atsushi、Ueno Yoshifumi、Cordeiro Andre M、Hara Toshihiko、Tanaka Ayumi、Akimoto Seiji、Oliveira M Margarida、Tanaka Ryouichi
    • Journal Title

      Journal of Experimental Botany

      Volume: 74 Issue: 1 Pages: 336-351

    • DOI

      10.1093/jxb/erac423

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Exposure to strong irradiance exacerbates photoinhibition and suppresses N resorption during leaf senescence in shade-grown seedlings of fullmoon maple (Acer japonicum)2022

    • Author(s)
      Kitao Mitsutoshi、Yazaki Kenichi、Tobita Hiroyuki、Agathokleous Evgenios、Kishimoto Junko、Takabayashi Atsushi、Tanaka Ryouichi
    • Journal Title

      Frontiers in Plant Science

      Volume: 13 Pages: 1006413-1006413

    • DOI

      10.3389/fpls.2022.1006413

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Characterization of photosystem II assembly complexes containing ONE-HELIX PROTEIN1 in Arabidopsis thaliana2022

    • Author(s)
      Maeda Hanaki、Takahashi Koharu、Ueno Yoshifumi、Sakata Kei、Yokoyama Akari、Yarimizu Kozue、Myouga Fumiyoshi、Shinozaki Kazuo、Ozawa Shin-Ichiro、Takahashi Yuichiro、Tanaka Ayumi、Ito Hisashi、Akimoto Seiji、Takabayashi Atsushi、Tanaka Ryouichi
    • Journal Title

      Journal of Plant Research

      Volume: 135 Issue: 2 Pages: 361-376

    • DOI

      10.1007/s10265-022-01376-x

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Presentation] 非モデル植物のシークエンス解析・インフォマティクス解析のコツ2023

    • Author(s)
      高林厚史
    • Organizer
      日本植物学会第87回大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 光捕集系の進化と光環境適応2022

    • Author(s)
      高林厚史
    • Organizer
      第12回日本光合成学会年会
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] 光化学系タンパク質複合体の安定な分離の ための CN-PAGE の改変2022

    • Author(s)
      亀尾辰砂,松前れのん,田中亮一,高林厚史
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Research-status Report
  • [Presentation] ツルマサキにおける α-carotene および lactucaxanthin の光化学系複合体への結合2022

    • Author(s)
      下原かのこ,高林厚史,田中亮一
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Research-status Report
  • [Remarks] 生物適応研究室ウエブページ

    • URL

      http://www.lowtem.hokudai.ac.jp/plantadapt/top-j.html

    • Related Report
      2023 Annual Research Report
  • [Remarks] 生物適応研究室

    • URL

      http://www.lowtem.hokudai.ac.jp/plantadapt/top-j.html

    • Related Report
      2022 Research-status Report 2021 Research-status Report

URL: 

Published: 2021-04-28   Modified: 2025-01-30  

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