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Physiological role of chloroplast DNA degradation during leaf senescence

Research Project

Project/Area Number 21K06230
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionOkayama University

Principal Investigator

Takami Tsuneaki  岡山大学, 総合技術部, 技術専門職員 (70614254)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2023: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2022: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2021: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
KeywordsオルガネラDNA / 葉緑体 / 光合成 / 老化
Outline of Research at the Start

本研究では、①葉の老化における葉緑体DNA分解によって生じるシグナル因子の特定と、②そのシグナル因子により引き起こされる老化遅延などの現象の詳細を明らかにし、研究代表者が提案するオルガネラDNA分解産物由来のシグナル因子による葉緑体と核の新たなクロストーク機構を検証する。加えてこれまでにゲノム編集で作出したイネ DPD1破壊株を新たな実験対象として検証実験を行う。

Outline of Final Research Achievements

Chloroplasts contain own DNA, and the amount of chloroplast DNA (cpDNA) is regulated in a tissue-specific manner and during leaf snescence. However, the physiological role of cpDNA degradation remains unclear. In addition, organelle exonuclease, DPD1, is widely conserved in seed plants, and it’s function may be diversified. In this study, we conducted several experiments using model plants Arabidopsis thaliana (dicot) and rice (monocot) for investigating the physiological role of cpDNA degradation. Our results suggested that organelle DNA degradation affects process of leaf senescence through regulating gene expression. Based on our results, the contribution of organelle DNA degradation for plant fitness is varied in plant species.

Academic Significance and Societal Importance of the Research Achievements

オルガネラDNA研究は酵母や藻類を用いて主に複製、安定性、修復などに関する知見が報告されているが、分解に焦点を当てた研究は少ない。このような研究動向の中で、本研究によってオルガネラDNA分解と植物の老化との関係性が明らかとなった。また単子葉類のイネを用いて研究を行った結果、オルガネラDNA分解酵素DPD1は広く種子植物に保存されているが、その寄与度は植物種により異なっていることが明らかとなった。これらの成果を基盤とすることで作物の育種・品種改良への応用が期待される。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (7 results)

All 2024 2023 2022

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (5 results)

  • [Journal Article] Plastid Inheritance Revisited: Emerging Role of Organelle DNA Degradation in Angiosperms2023

    • Author(s)
      Sakamoto Wataru、Takami Tsuneaki
    • Journal Title

      Plant and Cell Physiology

      Volume: - Issue: 4 Pages: 484-492

    • DOI

      10.1093/pcp/pcad104

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Maternal plastid inheritance: two abating factors identified2023

    • Author(s)
      Sakamoto Wataru、Takami Tsuneaki
    • Journal Title

      Trends in Genetics

      Volume: 39 Issue: 5 Pages: 342-343

    • DOI

      10.1016/j.tig.2023.03.002

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] オートファジー変異株早枯れ表現型のdpd1 変異による抑制に関するトランスクリプトーム解析2024

    • Author(s)
      高見常明,坂本亘
    • Organizer
      第65回日本植物生理学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Phenotypic and Transcriptomic characterization of the rice mutant defective in organelle exonuclease DPD12024

    • Author(s)
      Md Faridul Islam, Hiroshi Yamatani, Tsuneaki Takami, Makoto Kusaba, Wataru Sakamoto
    • Organizer
      第65回日本植物生理学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] オルガネラDNA分解異常が引き起こすオートファジー変異株早枯れ表現型の抑制機構について2023

    • Author(s)
      高見 常明、坂本 亘
    • Organizer
      第64回日本植物生理学会年会
    • Related Report
      2022 Research-status Report
  • [Presentation] Characterization of rice mutants lacking organelle exonuclease DPD12023

    • Author(s)
      Md. Faridul Islam, Hiroshi Yamatani, Tsuneaki Takami, Makoto Kusaba and Wataru Sakamoto
    • Organizer
      第64回日本植物生理学会年会
    • Related Report
      2022 Research-status Report
  • [Presentation] オルガネラDNA分解の異常はオートファジー変異体の早枯れ表現型を抑制する2022

    • Author(s)
      高見 常明
    • Organizer
      日本植物生理学会
    • Related Report
      2021 Research-status Report

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Published: 2021-04-28   Modified: 2025-01-30  

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