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Functional evolution of pyrenoids in chlorarachniophytes

Research Project

Project/Area Number 21K06285
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 45020:Evolutionary biology-related
Research InstitutionUniversity of Tsukuba

Principal Investigator

Hirakawa Yoshihisa  筑波大学, 生命環境系, 助教 (40647319)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2023: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2022: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2021: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords藻類 / 光合成 / 葉緑体 / 二酸化炭素 / ピレノイド / 収斂進化 / 二酸化炭素濃縮機構 / 液-液相分離 / オルガネラ進化 / 微細藻類
Outline of Research at the Start

ピレノイドは炭素固定酵素であるルビスコが集合した構造で、水中に生息する様々な藻類の葉緑体内で観察される(一般的に陸上植物には存在しない)。藻類はルビスコと二酸化炭素をピレノイドに集めることで、効率的に炭素固定を行っている。本研究では、海産の単細胞藻類を用いて、ピレノイドにルビスコが集まるメカニズムや二酸化炭素の濃縮機構を明らかにし、ピレノイドで機能する分子機構とその進化を解明する。

Outline of Final Research Achievements

Approximately one-third of the global carbon assimilation is estimated to occur within pyrenoids, which are widely present in eukaryotic algae. Pyrenoids are involved in CO2-concentrating mechanisms (CCMs) supplying CO2 to the carbon-fixing enzyme Rubisco. Although Rubisco is the predominant component of pyrenoids, other pyrenoid-associated proteins remain to be elucidated in most algae. To understand the diversity and evolution of pyrenoid-based CCMs among algae, we here investigated the pyrenoid proteome of a marine chlorarachniophyte alga. Our localization experiments demonstrated the specific targeting of eight proteins to pyrenoids. These included a putative Rubisco-binding linker, carbonic anhydrase, membrane transporter, and uncharacterized GTPase proteins. Notably, most of these proteins were unique to this algal lineage. This study provides insights into the convergent evolution of CO2-concentrating organelles at the molecular level.

Academic Significance and Societal Importance of the Research Achievements

海で固定化された二酸化炭素の一部は長期的に蓄積される「ブルーカーボン」と呼ばれ、大気中の二酸化炭素の削減に貢献するものとして注目されている。本研究では、海産の微細藻類が効率的に二酸化炭素を固定化するメカニズムの一端を明らかにした。これは、藻類に普遍的に存在するピレノイドの進化の謎に迫るだけでなく、ブルーカーボンの潜在的な可能性の理解に繋がる内容である。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (16 results)

All 2024 2023 2022 2021 Other

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (11 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Remarks (1 results)

  • [Journal Article] Pyrenoid proteomics reveals independent evolution of the CO2-concentrating organelle in chlorarachniophytes2024

    • Author(s)
      Moromizato Rena、Fukuda Kodai、Suzuki Shigekatsu、Motomura Taizo、Nagasato Chikako、Hirakawa Yoshihisa
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 121 Issue: 10

    • DOI

      10.1073/pnas.2318542121

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evolution of a chimeric mitochondrial carbonic anhydrase through gene fusion in a haptophyte alga2022

    • Author(s)
      Hirakawa Yoshihisa、Hanawa Yutaka、Yoneda Kohei、Suzuki Iwane
    • Journal Title

      FEBS Letters

      Volume: 596 Issue: 23 Pages: 3051-3059

    • DOI

      10.1002/1873-3468.14475

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] A membrane-bound cAMP receptor protein, SyCRP1 mediates inorganic carbon response in Synechocystis sp. PCC 68032022

    • Author(s)
      Bantu Lingaswamy、Chauhan Suraj、Srikumar Afshan、Hirakawa Yoshihisa、Suzuki Iwane、Hagemann Martin、Prakash Jogadhenu S.S.
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms

      Volume: 1865 Issue: 3 Pages: 194803-194803

    • DOI

      10.1016/j.bbagrm.2022.194803

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Characterization of a novel type of carbonic anhydrase that acts without metal cofactors2021

    • Author(s)
      Hirakawa Yoshihisa、Senda Miki、Fukuda Kodai、Yu Hong Yang、Ishida Masaki、Taira Masafumi、Kinbara Kazushi、Senda Toshiya
    • Journal Title

      BMC Biology

      Volume: 19 Issue: 1 Pages: 105-105

    • DOI

      10.1186/s12915-021-01039-8

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] クロララクニオン藻の葉緑体へのタンパク質輸送装置の探索2024

    • Author(s)
      山本健太,平川泰久
    • Organizer
      日本藻類学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] クロララクニオン藻Amorphochlora amoebiformisのゲノム解読2024

    • Author(s)
      青木大地,鈴木重勝,平川 泰久
    • Organizer
      日本藻類学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Proteomics reveals the evolution of chlorarachniophyte pyrenoids2023

    • Author(s)
      Yoshihisa Hirakawa
    • Organizer
      IX European Congress of Protistology & Annual Congress of the International Society of Protistologists
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 藻類から学ぶ二酸化炭素固定のライフハック2023

    • Author(s)
      平川泰久
    • Organizer
      日本植物学会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] クロララクニオン藻の葉緑体タンパク質輸送装置Tic20の局在2023

    • Author(s)
      山本健太,平川泰久
    • Organizer
      日本植物学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] クロララクニオン藻Amorphochlora amoebiformisのゲノム解読2023

    • Author(s)
      青木大地,鈴木重勝,平川 泰久
    • Organizer
      日本植物学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] クロララクニオン藻のプロテオームで紐解くピレノイドの進化2022

    • Author(s)
      平川泰久
    • Organizer
      日本植物学会
    • Related Report
      2022 Research-status Report
  • [Presentation] PI3P分子を指標とした藻類細胞のストレス動態と多様性2022

    • Author(s)
      大田修平、平川泰久、河地正伸
    • Organizer
      日本藻類学会
    • Related Report
      2021 Research-status Report
  • [Presentation] クロララクニオン藻におけるT7ファージ型DNAヘリカーゼの局在と進化2022

    • Author(s)
      青木大地,平川泰久
    • Organizer
      日本藻類学会
    • Related Report
      2021 Research-status Report
  • [Presentation] プロテオームで紐解くピレノイドの分子進化2022

    • Author(s)
      諸見里怜奈,平川泰久
    • Organizer
      日本藻類学会
    • Related Report
      2021 Research-status Report
  • [Presentation] クロララクニオン藻のピレノイド構築に関与するタンパク質の探索2021

    • Author(s)
      諸見里玲奈、 平川泰久
    • Organizer
      日本植物学会
    • Related Report
      2021 Research-status Report
  • [Remarks] 平川研究室HP

    • URL

      https://yhirakawa.weebly.com/

    • Related Report
      2023 Annual Research Report 2022 Research-status Report 2021 Research-status Report

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Published: 2021-04-28   Modified: 2025-01-30  

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