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Monoaminergic modulation of the oculomotor neural integrators

Research Project

Project/Area Number 21K06424
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 46030:Function of nervous system-related
Research InstitutionNara Medical University

Principal Investigator

Saito Yasuhiko  奈良県立医科大学, 医学部, 教授 (70290913)

Co-Investigator(Kenkyū-buntansha) 杉村 岳俊  奈良県立医科大学, 医学部, 助教 (60812526)
Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2023: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2022: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords視線制御 / セロトニン / ノルアドレナリン / 舌下神経前位核 / カハール間質核 / パッチクランプ法 / 神経積分器 / 脳スライス / ホールセル記録 / スライス標本
Outline of Research at the Start

脳幹の舌下神経前位核(PHN)やカハール間質核(INC)は視線制御に関与し、セロトニン(5-HT)やノルアドレナリン(NA)などのモノアミン作動性神経投射がみられる。本研究では、PHNやINCの機能に対する5-HTやNAの作用を明らかにするため、脳幹スライス標本においてPHMやINCのニューロンからホールセル記録を行い、初めの2年間で5-HTやNA投与に対する電流応答を詳細に調べ、3年目に興奮性ネットワーク活性に対する5-HTやNAの作用を調べる。

Outline of Final Research Achievements

The prepositus hypoglossi nucleus (PHN) and the interstitial nucleus of Cajal (INC), which are involved in the control of horizontal and vertical gaze, respectively. Because the effects of serotonin (5-HT) and noradrenaline (NA) on PHN and INC neurons and networks have not been fully clarified, we investigated current responses to 5-HT and NA in different neuron types using slice patch clamp methods. Local application of 5-HT and NA induced three and two different current types, which were caused by activation of different receptors. Furthermore, by examining current responses to 5-HT and NA in different neuron types classified based on the expression of different neurotransmitters, we found that each neuron type showed different current responses. These results clarified the mode of modulations by 5-HT and NA to the PHN and the INC.

Academic Significance and Societal Importance of the Research Achievements

視線制御のメカニズムの一つがPHNやINCでの神経修飾機構であり、それを担うものがセロトニンやノルアドレナリンなどのモノアミン作動性入力である。今回の研究により、モノアミンによるPHNやINCニューロンでの電流応答が明らかになり、特にこれまで報告が無かった5-HT3受容体を介する応答が明らかになった。今回の成果により、PHNやINCのニューロンでのモノアミンによる修飾様式が明らかになり、視線制御のメカニズムの解明が大きく進展するものと考えられる。さらに、脳で汎用的に用いられているモノアミンを介した情報変換機構への波及効果も期待される。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (16 results)

All 2024 2023 2022 2021 Other

All Journal Article (9 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 9 results,  Open Access: 4 results) Presentation (5 results) (of which Invited: 1 results) Remarks (2 results)

  • [Journal Article] Mitochondrial Calcium Uniporter (MCU) is Involved in an Ischemic Postconditioning Effect Against Ischemic Reperfusion Brain Injury in Mice2024

    • Author(s)
      Sasaki Hiromitsu、Nakagawa Ichiro、Furuta Takanori、Yokoyama Shohei、Morisaki Yudai、Saito Yasuhiko、Nakase Hiroyuki
    • Journal Title

      Cellular and Molecular Neurobiology

      Volume: 44 Issue: 1 Pages: 1-12

    • DOI

      10.1007/s10571-024-01464-7

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Serotonergic current responses of neurons in rat oculomotor neural integrators2023

    • Author(s)
      Saito Yasuhiko、Sugimura Taketoshi
    • Journal Title

      Journal of Neurophysiology

      Volume: 129 Issue: 2 Pages: 399-409

    • DOI

      10.1152/jn.00452.2022

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Behavioral and Immunohistochemical Evidence for Suppressive Effects of Goshajinkigan on Salicylate-Induced Tinnitus in Rats2022

    • Author(s)
      Kitano Koichi、Yamashita Akinori、Sugimura Taketoshi、Okayasu Tadao、Sakagami Masaharu、Osaki Daisuke、Kitahara Tadashi、Saito Yasuhiko
    • Journal Title

      Brain Sciences

      Volume: 12 Issue: 5 Pages: 587-587

    • DOI

      10.3390/brainsci12050587

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Neural Subtype-dependent Cholinergic Modulation of Neural Activities by Activation of Muscarinic 2 Receptors and G Protein-activated Inwardly Rectifying Potassium Channel in Rat Periaqueductal Gray Neurons2022

    • Author(s)
      Sugawara Shiori、Nakaya Yuka、Matsumura Sachie、Hirose Kensuke、Saito Yasuhiko、Kaneko Ryosuke、Kobayashi Masayuki
    • Journal Title

      Neuroscience

      Volume: 506 Pages: 1-13

    • DOI

      10.1016/j.neuroscience.2022.10.012

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Juvenile social isolation immediately affects the synaptic activity and firing property of fast-spiking parvalbumin-expressing interneuron subtype in mouse medial prefrontal cortex2022

    • Author(s)
      Okamura Kazuya、Yoshino Hiroki、Ogawa Yoichi、Yamamuro Kazuhiko、Kimoto Sohei、Yamaguchi Yasunari、Nishihata Yosuke、Ikehara Minobu、Makinodan Manabu、Saito Yasuhiko、Kishimoto Toshifumi
    • Journal Title

      Cerebral Cortex

      Volume: 33 Issue: 7 Pages: 3591-3606

    • DOI

      10.1093/cercor/bhac294

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Melatonin-Induced Postconditioning Suppresses NMDA Receptor through Opening of the Mitochondrial Permeability Transition Pore via Melatonin Receptor in Mouse Neurons2022

    • Author(s)
      Furuta Takanori、Nakagawa Ichiro、Yokoyama Shohei、Morisaki Yudai、Saito Yasuhiko、Nakase Hiroyuki
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 23 Issue: 7 Pages: 3822-3822

    • DOI

      10.3390/ijms23073822

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Distinct purinergic receptor-mediated currents of rat oculomotor integrator neurons characterized by different firing patterns2021

    • Author(s)
      Saito Yasuhiko、Sugimura Taketoshi
    • Journal Title

      Journal of Neurophysiology

      Volume: 126 Issue: 4 Pages: 1045-1054

    • DOI

      10.1152/jn.00209.2021

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Developmental dysregulation of excitatory-to-inhibitory GABA-polarity switch may underlie schizophrenia pathology: A monozygotic-twin discordant case analysis in human iPS cell-derived neurons2021

    • Author(s)
      Toritsuka Michihiro、Yoshino Hiroki、Makinodan Manabu、Ikawa Daisuke、Kimoto Sohei、Yamamuro Kazuhiko、Okamura Kazuya、Akamatsu Wado、Okada Yohei、Matsumoto Takuya、Hashimoto Kazumichi、Ogawa Yoichi、Saito Yasuhiko et al.
    • Journal Title

      Neurochemistry International

      Volume: 150 Pages: 105179-105179

    • DOI

      10.1016/j.neuint.2021.105179

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Differential regulation of medium spiny and cholinergic neurons in the nucleus accumbens core by the insular and medial prefrontal cortices in the rat2021

    • Author(s)
      Hirose Kensuke、Nakaya Yuka、Kitano Kohei、Saito Yasuhiko、Kaneko Ryosuke、Yanagawa Yuchio、Yamamoto Kiyofumi、Shirakawa Tetsuo、Kobayashi Masayuki
    • Journal Title

      Pfl?gers Archiv - European Journal of Physiology

      Volume: 473 Issue: 12 Pages: 1911-1924

    • DOI

      10.1007/s00424-021-02634-y

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Presentation] 小脳虫部または半球部に投射するラット舌下神経前位核の特徴的なニューロン群2023

    • Author(s)
      杉村岳俊、齋藤康彦
    • Organizer
      第46回日本神経科学大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Indirect pathway from the rat interstitial nucleus of Cajal to the vestibulo-cerebellum2023

    • Author(s)
      Taketoshi Sugimura, Yasuhiko Saito
    • Organizer
      日本生理学会100回大会
    • Related Report
      2022 Research-status Report
  • [Presentation] Effects of neuromodulation on local prefrontal circuits and the relevant behaviors in mice.2023

    • Author(s)
      Minobu Ikehara, Kazuhiko Yamamuro, Yuki Noriyama, Yasuhiko Saito, Manabu Makinodan
    • Organizer
      日本生理学会100回大会
    • Related Report
      2022 Research-status Report
  • [Presentation] 「視線保持のメカニズムからめまいの原因を紐解く」-神経積分器における興奮性回路の活性化機構について-2022

    • Author(s)
      齋藤康彦
    • Organizer
      第81回日本めまい平衡医学会総会・学術講演会
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] Indirect connection from the rat interstitial nucleus of Cajal to the vestibulo-cerebellum revealed by trans-synaptic tracing2021

    • Author(s)
      Taketoshi Sugimura, Yasuhiko Saito
    • Organizer
      第44回日本神経科学大会
    • Related Report
      2021 Research-status Report
  • [Remarks] 奈良県立医科大学第一生理学ホームページ

    • URL

      https://1phy.naramed-u.ac.jp/index.html

    • Related Report
      2023 Annual Research Report
  • [Remarks] 奈良県立医科大学 生理学第一講座ホームページ 業績欄

    • URL

      https://www.naramed-u.ac.jp/~1phy/company.html

    • Related Report
      2021 Research-status Report

URL: 

Published: 2021-04-28   Modified: 2025-01-30  

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