| Project/Area Number |
21K06612
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 47050:Environmental and natural pharmaceutical resources-related
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2023: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2022: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2021: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | long-lived animals / chitons / potential bacteria / metagenome / metagenomic analyses / sponge-derived compounds / Saccharomyces cerevisiae / proteasome activity / beneficial microbiomes / microbiomes / aquatic animals / biosynhetic genes / Bacterial cultivation / aquatic vertebrates / cultivation / biosynthesis / 3-D cultivation |
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| Outline of Research at the Start |
The important role of gut microbiomes in the prolonged lifespan of eukaryotic hosts has recently been recognized. Our proposed research aims to identify key bacteria, metabolites/natural products, and biosynthetic genes responsible for longevity and healthy lifespan in long-lived aquatic vertebrates. We believe that this has great scientific prospect, because natural products with anti-ageing properties can be developed as pharmaceutical agents that slow ageing or treat age-related diseases, thereby contributing to human healthy lifespan.
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| Outline of Final Research Achievements |
To identify key bacteria, metabolites and biosynthetic genes that may contribute to the longevity of aquatic animals, we initially obtained bacterial isolates from long-lived animals, such as chitons (lifespan ~40 years). We isolated ~430 bacterial strains from different parts of individual chitons. Strains showing resistance to certain antibiotics were subjected to PCR-cloning to detect those with biosynthetic potential. Subsequent metagenomic analyses led to the identification of biosynthetically promising bacteria in two of six studied chiton samples. This is in collaboration with Piel Lab (ETH Zurich) to complement their work on bacterial chemistry (Chem 2023, 9, 12: 3696-713). Using known sponge-derived compounds, we optimized the screening protocol for testing the ability of such compounds to stimulate lifespan in the organism model Saccharomyces cerevisiae. This was based on monitoring proteasome activity of compound-treated yeast lysates.
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| Academic Significance and Societal Importance of the Research Achievements |
Through metagenomic analyses, we identified biosynthetically promising chiton-associated acidobacteria with the ability to produce bioactive sponge-type natural products. This could be a basis for exploring the biosynthetic potential of acidobacterial members from diverse environmental types.
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